Open Close
General Information
Symbol
Dmel\RhoLN25.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0061655
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-RhoLN25
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of RhoL that carries the amino acid replacement T25N. This is expected to result in a dominant-negative protein (the analagous change in Ras causes preferential binding of GDP compared to GTP).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of RhoLN25.Scer\UAS under the control of Scer\GAL4elav.PU (using tub-Gal80[ts] to limit the expression to the adult stage) does not significantly alter 3 hr memory of adult flies in an aversive olfactory assay compared to controls.

Expression of RhoLN25.Scer\UAS in border cells, driven by Scer\GAL4slbo.2.6, causes a border cell cluster migration defect in 30% of egg chambers.

Expression of RhoLN25.Scer\UAS under the control of Scer\GAL4gcm-rA87.P or Scer\GAL4da.G32 does not interfere with macrophage migration in the embryo.

RhoLN25.Scer\UAS when driven by Scer\GAL4nos.UTR.T:Hsim\VP16 has no effect on transepithelial migration of germ cells in the embryo.

Scer\GAL4-mediated expression fails to cause any border cell migration defects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of RhoLN25.Scer\UAS does not suppress the rough eye phenotype seen when pblDH-PH.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Generates a dominant negative protein.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
RhoLN25.Scer\UAS
RhoLN25.UAS
Name Synonyms
Secondary FlyBase IDs
    References (7)