FlyBase Allele Report: Dmel\Cdc42[4]

General Information

Symbol

Dmel\Cdc42⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0062063

Feature type

allele

Associated gene

Dmel\Cdc42

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Scott et al., 2003)

Mutagen

ethyl methanesulfonate

(Fehon et al., 1997)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G370A. Mutation in splice acceptor consensus site.

(Genova et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:19,698,306..19,698,306 [+]

Nucleotide change:

G19698306A

Reported nucleotide change:

G370A

(Genova et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

After single cell wounding (by laser ablation), Cdc42⁴/Cdc42⁶ embryos show a wider actin ring than wild type and less orientated cortical actin flow toward the wound. Wound expansion and actin halo formation are not significantly affected.

(Abreu-Blanco et al., 2014)

Cdc42⁴/Cdc42⁶ embryos show defects in epithelial wound repair. The expansion and coalescence phases are not significantly delayed and by contraction, the wounds have become rounded, consistent with the presence of a functional actomyosin cable. There is a severe reduction of filopodia and lamellipodia protrusions in the leading edge cells throughout the wound repair process. The contraction phase is doubled in length compared to wild type. The wound cannot be sealed and a small hole remains at the end of the process.

(Abreu-Blanco et al., 2012)

26% of Cdc42⁴ mutant R cell clones target normally, whereas 23% select inappropriate target columns and 51% fail to extend. Some photoreceptors display morphological defects in the retina, although this phenotype did not correlate with the axon targeting phenotype.

(Gontang et al., 2011)

Homozygous tracheal terminal cells have very strong defects in branching and lumen formation.

(Jones and Metzstein, 2011)

Heterozygous and Cdc42⁴/Cdc42² flies show normal resistance to infection with P.aeruginosa by septic injury.

(Avet-Rochex et al., 2007)

VS1 neurons that are homozygous for Cdc42³ often have defects in dendrite caliber; distal dendrites can be thicker than proximal dendrites and dendrites can also be thinner near major branches than wild-type. The dendrites of Cdc42³ cells are also often misguided: for example, a dendrite can innervate the ventral region of the dendritic field, even though it has originated from a dorsal branch. Additionally, the most medial branch in the dendritic tree is shifted medially, resulting in a more pronounced split between the dorsal and ventral halves of the dendritic field. The axonal terminus of a Cdc42³ VS1 neuron has an increase in the number of branches, is often tipped with enlarged structures and can have axons that turn laterally away from the midline. Dendrites are significantly longer in mutants than in wild type and there is a 50% reduction in numbers of well-developed dendritic spines.

(Scott et al., 2003)

Cdc42⁴/Cdc42⁶ combinations produce over 70% embryonic lethality, even when outcrossed to wild-type males. In Cdc42⁴/Cdc42⁶ germ-line clones, the specialised actin filaments that project outward from the nurse cell ring canals appear to be enhanced. Although Cdc42⁴ germ-line clones fail to produce functional oocytes, their anterior posterior polarity appears to be unaffected.

(Genova et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Cdc42⁴ has abnormal neuroanatomy | somatic clone phenotype, non-suppressible by Scer\GAL4^elav-C155/gek^UAS.cGa

(Gontang et al., 2011)

Enhancer of

Statement

Reference

Cdc42[+]/Cdc42⁴ is an enhancer of abnormal planar polarity phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

NOT Enhancer of

Statement

Reference

Cdc42[+]/Cdc42⁴ is a non-enhancer of abnormal neurophysiology | third instar larval stage | recessive phenotype of GluRIIA^SP16

(Frank et al., 2009)

Cdc42[+]/Cdc42⁴ is a non-enhancer of abnormal neuroanatomy phenotype of PsGEF^Δ21

(Higuchi et al., 2009)

Cdc42[+]/Cdc42⁴ is a non-enhancer of visible | dominant phenotype of Sb⁷⁰

(Chen et al., 2004)

Cdc42[+]/Cdc42⁴ is a non-enhancer of visible | dominant phenotype of Sb^63b

(Chen et al., 2004)

Cdc42⁴ is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, pbl^UAS.cPa

(Prokopenko et al., 1999)

Suppressor of

Statement

Reference

Cdc42⁴ is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of Dyb¹¹

(Jantrapirom et al., 2019)

Cdc42[+]/Cdc42⁴ is a suppressor of abnormal neuroanatomy phenotype of Pak^UAS.Tag:MYC, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Cdc42[+]/Cdc42⁴ is a suppressor of abnormal neuroanatomy | somatic clone phenotype of ssh^1-11

(Ng and Luo, 2004)

Cdc42[+]/Cdc42⁴ is a suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

NOT Suppressor of

Statement

Reference

Cdc42⁴/Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^eve.RN2 is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^eve.RN2, dock^JF02810

(Kamiyama et al., 2015)

Cdc42⁴/Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^eve.RN2 is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of Dscam1^HMS01859, Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

Cdc42[+]/Cdc42⁴ is a non-suppressor of abnormal neuroanatomy | heat sensitive phenotype of N^l1N-ts1

(Song and Giniger, 2011)

Cdc42[+]/Cdc42⁴ is a non-suppressor of abnormal neurophysiology | recessive | third instar larval stage phenotype of GluRIIA^SP16

(Frank et al., 2009)

Cdc42[+]/Cdc42⁴ is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PU, pbl^{DH-PH.UAS.Tag:HA}

(van Impel et al., 2009)

Cdc42⁴ is a non-suppressor of visible phenotype of LIMK1^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2004)

Cdc42⁴ is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, pbl^UAS.cPa

(Prokopenko et al., 1999)

Other

Statement

Reference

Cdc42[+]/Cdc42⁴, Exn^EY01953/Exn[+], GluRIIA^SP16 has abnormal neurophysiology | recessive | third instar larval stage phenotype

(Frank et al., 2009)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Cdc42⁴ has eye photoreceptor cell | somatic clone phenotype, non-suppressible by Scer\GAL4^elav-C155/gek^UAS.cGa

(Gontang et al., 2011)

Enhancer of

Statement

Reference

Cdc42[+]/Cdc42⁴ is an enhancer of ommatidium phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

Cdc42⁴ is an enhancer of mesonotum phenotype of Pvr^RNAi.UAS, Scer\GAL4^pnr-MD237

(Ishimaru et al., 2004)

Cdc42⁴ is an enhancer of thorax phenotype of Pvr^RNAi.UAS, Scer\GAL4^pnr-MD237

(Ishimaru et al., 2004)

NOT Enhancer of

Statement

Reference

Cdc42[+]/Cdc42⁴ is a non-enhancer of embryonic/larval neuromuscular junction phenotype of GluRIIA^SP16

(Frank et al., 2009)

Cdc42[+]/Cdc42⁴ is a non-enhancer of adult mushroom body alpha-lobe phenotype of PsGEF^Δ21

(Higuchi et al., 2009)

Cdc42[+]/Cdc42⁴ is a non-enhancer of leg phenotype of Sb⁷⁰

(Chen et al., 2004)

Cdc42[+]/Cdc42⁴ is a non-enhancer of leg phenotype of Sb^63b

(Chen et al., 2004)

Cdc42[+]/Cdc42⁴ is a non-enhancer of mitotic domain 1 | embryonic cycle 14 phenotype of CycB^+t10

(Ji et al., 2002)

Cdc42⁴ is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, pbl^UAS.cPa

(Prokopenko et al., 1999)

Suppressor of

Statement

Reference

Cdc42⁴ is a suppressor of A1-7 ventral longitudinal muscle cell | larval stage phenotype of Dyb¹¹

(Jantrapirom et al., 2019)

Cdc42[+]/Cdc42⁴ is a suppressor of adult mushroom body phenotype of Pak^UAS.Tag:MYC, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Cdc42[+]/Cdc42⁴ is a suppressor of adult mushroom body | somatic clone phenotype of ssh^1-11

(Ng and Luo, 2004)

Cdc42[+]/Cdc42⁴ is a suppressor of adult mushroom body phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

NOT Suppressor of

Statement

Reference

Cdc42⁴/Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^eve.RN2 is a non-suppressor of larval DA1 motor neuron | embryonic stage phenotype of Dscam1^HMS01859, Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

Cdc42⁴/Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^eve.RN2 is a non-suppressor of dendrite | embryonic stage phenotype of Dscam1^HMS01859, Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

Cdc42⁴/Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^eve.RN2 is a non-suppressor of larval DA1 motor neuron | embryonic stage phenotype of Scer\GAL4^eve.RN2, dock^JF02810

(Kamiyama et al., 2015)

Cdc42⁴/Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^eve.RN2 is a non-suppressor of dendrite | embryonic stage phenotype of Scer\GAL4^eve.RN2, dock^JF02810

(Kamiyama et al., 2015)

Cdc42[+]/Cdc42⁴ is a non-suppressor of larval intersegmental nerve | heat sensitive phenotype of N^l1N-ts1

(Song and Giniger, 2011)

Cdc42[+]/Cdc42⁴ is a non-suppressor of larval intersegmental nerve branch ISNb of A1-7 | heat sensitive phenotype of N^l1N-ts1

(Song and Giniger, 2011)

Cdc42[+]/Cdc42⁴ is a non-suppressor of larval segmental nerve branch SNa of A1-7 | heat sensitive phenotype of N^l1N-ts1

(Song and Giniger, 2011)

Cdc42[+]/Cdc42⁴ is a non-suppressor of embryonic/larval neuromuscular junction phenotype of GluRIIA^SP16

(Frank et al., 2009)

Cdc42[+]/Cdc42⁴ is a non-suppressor of eye | adult stage phenotype of Scer\GAL4^GMR.PU, pbl^{DH-PH.UAS.Tag:HA}

(van Impel et al., 2009)

Cdc42⁴ is a non-suppressor of wing phenotype of LIMK1^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2004)

Cdc42[+]/Cdc42⁴ is a non-suppressor of mitotic domain 1 | embryonic cycle 14 phenotype of CycB^+t10

(Ji et al., 2002)

Cdc42⁴ is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, pbl^UAS.cPa

(Prokopenko et al., 1999)

Other

Statement

Reference

Cdc42[+]/Cdc42⁴, Exn^EY01953/Exn[+], GluRIIA^SP16 has embryonic/larval neuromuscular junction phenotype

(Frank et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Cdc42⁴ heterozygosity suppresses the quantal content and evoked junctional potential phenotypes of Dyb¹¹ heterozygotes.

(Jantrapirom et al., 2019)

The dendritogenesis defects in the embryonic aCC motoneurons characteristic for embryos expressing either Dscam1^HMS01859 or dock^JF02810 RNAi under the control of Scer\GAL4^eve.RN2 are partially suppressed by co-expression of Pak^{Scer\UAS.T:Myr-Src64B} : the reduced dendritic number is fully restored but the region containing primary dendritic processes is expanded compared to controls. This rescue effect is however blocked in the presence of either Cdc42⁶ or Cdc42⁴ (in hemizygous state). No suppression of the loss of dendritic tips is observed upon co-expression with Pak^{Scer\UAS.T:Lhem\EosFP-m2}.

(Kamiyama et al., 2015)

Expression of gek^Scer\UAS.cGa under the control of Scer\GAL4^elav-C155 fails to suppress the axon targeting phenotypes seen in Cdc42⁴ mutant clones.

(Gontang et al., 2011)

Heterozygosity for Cdc42⁴ in a cv-c¹/+ mutant background restores quantal content (QC) to wild-type levels.

Heterozygosity for Cdc42⁴ in a Dys^E6/+ mutant background restores quantal content (QC) to wild-type levels.

(Pilgram et al., 2011)

Heterozygosity for Cdc42⁴ fails to suppress the axonal defects found in N^l1N-ts1 mutants.

(Song and Giniger, 2011)

The Cdc42⁴/+ heterozygous mutation does not suppress or enhance the GluRIIA^SP16 NMJ synaptic homeostasis phenotype. There is no deficit in synaptic bouton number in Cdc42⁴/+ , GluRIIA^SP16 double mutants, compared to wild-type or GluRIIA^SP16 controls.

Synaptic homeostasis is completely blocked in larvae that are double heterozygotes for Cdc42⁴/+ and Exn^EY01953/+ in a GluRIIA^SP16 mutant genetic background.

(Frank et al., 2009)

Heterozygosity for Cdc42⁴ has no effect on the phenotype in the alpha lobes of the mushroom bodies that is seen in PsGEF^Δ21 animals.

(Higuchi et al., 2009)

Expression of Rho1^{1.dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PI does not affect the adherens junctions between Cdc42⁴ mutant cells.

(Warner and Longmore, 2009)

One copy of Cdc42⁴ fails to suppress the rough eye phenotype seen when pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^GMR.PU.

(van Impel et al., 2009)

The Cdc42⁴ mutation fails to modify the cv-c^M62 phenotype in the Malpighian tubules.

(Denholm et al., 2005)

Cdc42⁴/+ has very little effect on the mutant wing phenotype caused by expression of LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E (the % of wings with normal morphology at 18^oC is 10% compared to 9% for control flies expressing LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E in an otherwise wild-type background). The frequency of the malformed leg phenotype seen in Sb^63b/+ heterozygotes (8%) is not increased if the flies are also heterozygous for Cdc42⁴/+ (6%). The frequency of the malformed leg phenotype seen in Sb⁷⁰/+ heterozygotes (7%) is not increased if the flies are also heterozygous for Cdc42⁴/+ (8%).

(Chen et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Cdc42⁶

(Genova et al., 2000)

Rescued by

Cdc42⁴ is rescued by Cdc42^{Ubi-p63E.Tag:MYC}

(Genova et al., 2000)

Cdc42⁴ is rescued by Cdc42^Ubi-p63E.PF

(Fehon et al., 1997)

Partially rescued by

Cdc42⁴ is partially rescued by Cdc42^UAS.cLa/Scer\GAL4^elav.PLu

(Scott et al., 2003)

Comments

Expression of Cdc42^Scer\UAS.cLa under the control of Scer\GAL4^elav.PLu rescues the dendritic caliber and branching pattern phenotype of Cdc42⁴ mutants in VS1 neurons. However, this expression is unable to rescue many of the VS1 defects such as dendrites being significantly longer than in wild-type and reduction of well developed dendritic spines.

(Scott et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9106

y¹ w^* Cdc42⁴ P{neoFRT}19A/FM6

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AF153427

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAD43791

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Name Synonyms

Secondary FlyBase IDs

References (37)

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