FlyBase Allele Report: Dmel\Cdc42[3]

General Information

Symbol

Dmel\Cdc42³

Species

D. melanogaster

Name

FlyBase ID

FBal0062064

Feature type

allele

Associated gene

Dmel\Cdc42

Associated Insertion(s)

Carried in Construct

Also Known As

cdc42-3

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Stramer et al., 2005, Scott et al., 2003)

Mutagen

ethyl methanesulfonate

(Fehon et al., 1997)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G423A. Amino acid replacement: G114D.

(Genova et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:19,698,359..19,698,359 [+]

Nucleotide change:

G19698359A

Reported nucleotide change:

G423A

Amino acid change:

G114D | Cdc42-PA; G114D | Cdc42-PC; G114D | Cdc42-PD

Reported amino acid change:

G114D

(Genova et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryo | anterior | maternal effect (with Cdc42⁶)

(Genova et al., 2000)

embryo | dorsal closure stage | maternal effect (with Cdc42⁶)

(Genova et al., 2000)

embryo | maternal effect (with Cdc42⁶)

(Genova et al., 2000)

embryonic/first instar larval cuticle | maternal effect | ventral (with Cdc42⁶)

(Genova et al., 2000)

embryonic/larval hemocyte (with Cdc42⁶)

(Stramer et al., 2005)

embryonic epidermis | maternal effect | ventral (with Cdc42⁶)

(Genova et al., 2000)

embryonic proventriculus

(Fuss et al., 2004)

ganglionic tracheal branch primordium

(Lundstrom et al., 2004)

larval longitudinal connective | maternal effect (with Cdc42⁶)

(Genova et al., 2000)

neuroblast | third instar larval stage

(Atwood et al., 2007)

nurse cell & actin filament | germ-line clone | maternal effect

(Genova et al., 2000)

ommatidium | somatic clone

(Genova et al., 2000)

oocyte | germline clone

(Leibfried et al., 2013)

presumptive embryonic/larval central nervous system | maternal effect (with Cdc42⁶)

(Genova et al., 2000)

proventriculus inner layer primordium

(Fuss et al., 2004)

proventriculus intermediate layer primordium

(Fuss et al., 2004)

wing hair | somatic clone | increased number

(Fricke et al., 2009)

Detailed Description

Statement

Reference

Homozygous germline clones result in the arrest of oogenesis and mutant egg chambers develop only until stage 5. From stage 4 onwards, the mutant egg chambers contain 16 polyploid nurse cells instead of the normal 15 nurse cells plus one oocyte. Marker analysis indicates that the oocyte is correctly specified during early stages, but that its fate is not maintained.

(Leibfried et al., 2013)

Cdc42³/+ flies do not exhibit a significantly increased pacing-induced heart failure rate or arrhythmia, do not have significantly different diastolic or systolic intervals, nor any significant disruption in cellular architecture of cardiomyocytes as compared to controls.

(Qian et al., 2011)

Homozygous clones in the wing result in a multiple wing hair phenotype.

(Fricke et al., 2009)

Cdc42³ mutants have normal embryonic and early larval neuroblast polarity. Third instar larval central brain neuroblasts display polarity defects.

(Atwood et al., 2007)

Homozygous Cdc42³ mutant embryos do not exhibit a myoblast fusion phenotype.

(Schafer et al., 2007)

Hemocytes in embryos dervied from Cdc42³/Cdc42⁶ mothers show normal developmental dispersal and normal recruitment to sites of laser-induced tissue damage. However, during the migratory phase and after arrival at the wound site, the mutant hemocytes often possess several leading edges suggesting that they cannot maintain a persistent polarity.

The failure of hemocytes to maintain polarity in embryos dervied from Cdc42³/Cdc42⁶ mothers leads the hemocytes to adopt a haphazard migratory route. This defect is countered by the mutant hemocytes migrating at approximately twice the normal speed so that they reach the wound as rapidly as in wild type embryos. On reaching the wound, the mutant hemocytes appear to remain more active than wild type hemocytes, and exhibit exuberant protrusive activity.

(Stramer et al., 2005)

In Cdc42³/Cdc42³ embryos the ectodermal keyhole cells fail to invaginate into the proventricular endoderm.

(Fuss et al., 2004)

Mutant embryos exhibit arrested ganglionic branches (GBs), and GBs turning prematurely away from the midline.

(Lundstrom et al., 2004)

Cdc42³/Cdc42⁶ combinations produce over 70% embryonic lethality, even when outcrossed to wild-type males. Embryos produced by Cdc42³/Cdc42⁶ mothers display disruptions in epidermal development, including incomplete germband retraction, ventral holes in the epidermis, ventral holes in cuticle produced by epidermal cells, and anterior open phenotypes. Ventral holes range in size from isolated disruptions to openings in the entire ventral surface of the embryo. Patterns of neuronal differentiation are largely normal, even in areas where the integrity of the overlying epidermis is disrupted. In certain embryos defects in the central nervous system, such as incomplete formation and midline fusion of the longitudinal connectives are observed. However, in all embryos the axons of the peripheral nervous system appear to extend properly. Cdc42³ somatic clones generated early in larval development do not survive until the wandering third instar larval stage, those generated later (72h AEL) are smaller than sister control clones. No clones survive to adulthood. Even in a Minute background clones in the eye are only visible as scars, not producing any adult ommatidia. Clones in the eye disc do initiate differentiation as photoreceptor cells in the larval eye imaginal disc. In Cdc42³ germ-line clones, the cytoplasmic actin filaments that normally form in the nurse cells, although still present are reduced in number compared to wild-type.

(Genova et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Cdc42[+]/Cdc42³ is an enhancer of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

NOT Enhancer of

Statement

Reference

Cdc42[+]/Cdc42³ is a non-enhancer of abnormal neuroanatomy phenotype of DAAM^Ex1

(Gombos et al., 2015)

Cdc42³ is a non-enhancer of abnormal neuroanatomy phenotype of SNF4Aγ^loe

(Cook et al., 2012)

Cdc42[+]/Cdc42³ is a non-enhancer of abnormal neurophysiology | third instar larval stage | recessive phenotype of GluRIIA^SP16

(Frank et al., 2009)

Cdc42[+]/Cdc42³ is a non-enhancer of visible | dominant phenotype of Sb⁷⁰

(Chen et al., 2004)

Cdc42[+]/Cdc42³ is a non-enhancer of visible | dominant phenotype of Sb^63b

(Chen et al., 2004)

Cdc42³ is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, pbl^UAS.cPa

(Prokopenko et al., 1999)

Suppressor of

Statement

Reference

Cdc42³ is a suppressor of visible | adult stage phenotype of Hsap\TTR^{V30M.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Oliveira da Silva et al., 2020)

Cdc42[+]/Cdc42³ is a suppressor of abnormal planar polarity phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

Cdc42[+]/Cdc42³ is a suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Cdc42³ is a suppressor of visible phenotype of Scer\GAL4^en-e16E, tum^ΔE1E.UAS

(Sotillos and Campuzano, 2000)

NOT Suppressor of

Statement

Reference

Cdc42³ is a non-suppressor of abnormal neuroanatomy phenotype of SNF4Aγ^loe

(Cook et al., 2012)

Cdc42[+]/Cdc42³ is a non-suppressor of abnormal neurophysiology | recessive | third instar larval stage phenotype of GluRIIA^SP16

(Frank et al., 2009)

Cdc42³ is a non-suppressor of visible phenotype of LIMK1^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2004)

Cdc42³ is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, pbl^UAS.cPa

(Prokopenko et al., 1999)

Other

Statement

Reference

Cdc42[+]/Cdc42³, Exn^EY01953/Exn[+], GluRIIA^SP16 has abnormal neurophysiology | recessive | third instar larval stage phenotype

(Frank et al., 2009)

Cdc42[+]/Cdc42³, GluRIIA^SP16, cac^S/cac[+] has abnormal neurophysiology | recessive | third instar larval stage phenotype

(Frank et al., 2009)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Cdc42³ has ganglionic tracheal branch primordium phenotype, non-suppressible by RhoGAP93B¹

(Lundstrom et al., 2004)

Enhancer of

Statement

Reference

Cdc42[+]/Cdc42³ is an enhancer of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

NOT Enhancer of

Statement

Reference

Cdc42[+]/Cdc42³ is a non-enhancer of adult mushroom body alpha-lobe phenotype of DAAM^Ex1

(Gombos et al., 2015)

Cdc42[+]/Cdc42³ is a non-enhancer of adult mushroom body beta-lobe phenotype of DAAM^Ex1

(Gombos et al., 2015)

Cdc42[+]/Cdc42³ is a non-enhancer of embryonic/larval neuromuscular junction phenotype of GluRIIA^SP16

(Frank et al., 2009)

Cdc42³ is a non-enhancer of photoreceptor cell | precursor & nucleus phenotype of Scer\GAL4^unspecified, msn^DN.UAS

(Houalla et al., 2005)

Cdc42[+]/Cdc42³ is a non-enhancer of leg phenotype of Sb⁷⁰

(Chen et al., 2004)

Cdc42[+]/Cdc42³ is a non-enhancer of leg phenotype of Sb^63b

(Chen et al., 2004)

Cdc42³ is a non-enhancer of larval VL3/4 motor neuron phenotype of PlexB^UAS.cHa, Scer\GAL4^elav.PLu

(Hu et al., 2001)

Cdc42³ is a non-enhancer of larval intersegmental nerve phenotype of PlexB^UAS.cHa, Scer\GAL4^elav.PLu

(Hu et al., 2001)

Cdc42³ is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, pbl^UAS.cPa

(Prokopenko et al., 1999)

Suppressor of

Statement

Reference

Cdc42³ is a suppressor of eye phenotype of Hsap\TTR^{V30M.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Oliveira da Silva et al., 2020)

Cdc42[+]/Cdc42³ is a suppressor of ommatidium phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

Cdc42[+]/Cdc42³ is a suppressor of adult mushroom body phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Cdc42³ is a suppressor of wing vein phenotype of Scer\GAL4^en-e16E, tum^ΔE1E.UAS

(Sotillos and Campuzano, 2000)

Cdc42³ is a suppressor of wing phenotype of Scer\GAL4^en-e16E, tum^ΔE1E.UAS

(Sotillos and Campuzano, 2000)

NOT Suppressor of

Statement

Reference

Cdc42[+]/Cdc42³ is a non-suppressor of embryonic/larval neuromuscular junction phenotype of GluRIIA^SP16

(Frank et al., 2009)

Cdc42³ is a non-suppressor of wing phenotype of LIMK1^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2004)

Cdc42³ is a non-suppressor of larval VL3/4 motor neuron phenotype of PlexB^UAS.cHa, Scer\GAL4^elav.PLu

(Hu et al., 2001)

Cdc42³ is a non-suppressor of larval intersegmental nerve phenotype of PlexB^UAS.cHa, Scer\GAL4^elav.PLu

(Hu et al., 2001)

Cdc42³ is a non-suppressor of ommatidium phenotype of Rac1^V12.hs.sev

(Fanto et al., 2000)

Cdc42³ is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, pbl^UAS.cPa

(Prokopenko et al., 1999)

Cdc42³ is a non-suppressor of phenotype of dsh^hs.sev.B

(Boutros et al., 1998)

Other

Statement

Reference

Cdc42³, Pak[+]/Pak⁶ has adult heart phenotype

(Qian et al., 2011)

Cdc42³, Pak[+]/Pak⁶ has cardial cell phenotype

(Qian et al., 2011)

Cdc42[+]/Cdc42³, tin³⁴⁶ has adult heart phenotype

(Qian et al., 2011)

Cdc42[+]/Cdc42³, tin³⁴⁶ has cardial cell phenotype

(Qian et al., 2011)

Cdc42[+]/Cdc42³, tin³⁴⁶ has cardiac myofibril phenotype

(Qian et al., 2011)

Cdc42[+]/Cdc42³, tin^EC40 has adult heart phenotype

(Qian et al., 2011)

Cdc42³, Df(2L)Exel9032/+ has adult heart phenotype

(Qian et al., 2011)

Cdc42³, Df(2L)Exel9032/+ has cardial cell phenotype

(Qian et al., 2011)

Cdc42³, Df(3R)BSC397/+ has adult heart phenotype

(Qian et al., 2011)

Cdc42³, Df(3R)BSC397/+ has cardial cell phenotype

(Qian et al., 2011)

Cdc42[+]/Cdc42³, Exn^EY01953/Exn[+], GluRIIA^SP16 has embryonic/larval neuromuscular junction phenotype

(Frank et al., 2009)

Cdc42[+]/Cdc42³, GluRIIA^SP16, cac^S/cac[+] has embryonic/larval neuromuscular junction phenotype

(Frank et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of Cdc42³ does not enhance the axonal growth defects seen in the α and β lobes of DAAM^Ex1 mutant mushroom bodies.

(Gombos et al., 2015)

There is no significant difference in the nervous system degeneration seen in SNF4Aγ^loe mutants in a Cdc42³ heterozygous background.

(Cook et al., 2012)

Cdc42³/+; Pak⁶/+ double heterozygotes display severe cardiomyocyte organization abnormalities and contractile abnormalities as compared with either single heterozygote, but no differences in heart beat defects and overall rhythmicity.

Cdc42³/+; Df(2L)Exel6012/+ double heterozygotes do not display defects in heart rhythm as compared to controls.

Cdc42³/+; Df(2L)Exel9032/+ double heterozygotes and Cdc42³/+; Df(3R)BSC397/+ double heterozygotes show significant defects in cardiomyocyte structure, significantly increased arrhythmia, but no significant differences in the overall length of diastolic and systolic intervals.

tin³⁴⁶/+; Cdc42³/+ double heterozygotes exhibit a significantly increased pacing-induced heart failure rate, significantly increased arrhythmia and a tendency toward longer diastolic intervals, in addition to severe misalignment of cardiac myofibrils, as compared with controls. These flies do not exhibit differences in systolic intervals.

tin^EC40/+; Cdc42³/+ double heterozygotes exhibit a significantly increased pacing-induced heart failure rate.

(Qian et al., 2011)

The Cdc42³/+ heterozygous mutation does not suppress or enhance the GluRIIA^SP16 NMJ synaptic homeostasis phenotype. There is no deficit in synaptic bouton number in Cdc42³/+ , GluRIIA^SP16 double mutants, compared to wild-type or GluRIIA^SP16 controls.

Synaptic homeostasis is completely blocked in larvae that are double heterozygotes for Cdc42³/+ and Exn^EY01953/+ in a GluRIIA^SP16 mutant genetic background. There is no deficit in synaptic bouton number in Cdc42³/+ , Exn^EY01953/+, GluRIIA^SP16 triple mutants, compared to wild-type or GluRIIA^SP16 controls.

The combination of Cdc42³/+ and cac^S/+ heterozygous mutations blocks the synaptic homeostatic compensation at the neuromuscular junction (NMJ) in a homozygous GluRIIA^SP16 genetic background.

(Frank et al., 2009)

Cdc42³/+ has very little effect on the mutant wing phenotype caused by expression of LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E (the % of wings with normal morphology at 18^oC is 10% compared to 9% for control flies expressing LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E in an otherwise wild-type background). The frequency of the malformed leg phenotype seen in Sb^63b/+ heterozygotes (8%) is not increased if the flies are also heterozygous for Cdc42³/+ (3%). The frequency of the malformed leg phenotype seen in Sb⁷⁰/+ heterozygotes (7%) is not increased if the flies are also heterozygous for Cdc42³/+ (7%).

(Chen et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Cdc42⁶

(Genova et al., 2000)

Rescued by

Cdc42³ is rescued by Cdc42^{Ubi-p63E.Tag:MYC}

(Genova et al., 2000)

Cdc42³ is rescued by Cdc42^Ubi-p63E.PF

(Fehon et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7337

w¹ sn³ Cdc42³/FM6

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AF153426

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAD43790

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Name Synonyms

Secondary FlyBase IDs

References (32)

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