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General Information
Symbol
Dmel\Cdc422
Species
D. melanogaster
Name
FlyBase ID
FBal0062065
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

T19698247A

Reported nucleotide change:

T311A

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: T311A. Mutation in splice donor consensus site.

Mutation in the splice donor sequence of the small internal intron.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Cdc422/Y males have mushroom body axon growth and guidance defects.

The hatching rate of eggs derived from homozygous females is reduced compared to wild type, regardless of the zygotic state.

Mutant larvae fail to encapsulate eggs when parasitised by the avirulent L. boulardi wasp strain G486 (no melanisation is seen). Plasmatocytes and lamellocytes fully cover the egg.

Homozygous larvae fail to encapsulate eggs of the avirulent wasp strain L. boulardi G486.

Isolated plasmatocytes from homozygous larvae show a reduced ability to phagocytose E. coli and S. aureus in a phagocytosis assay.

Cdc422 heterozygous larvae exhibit wild-type neuromuscular junction length and total bouton number, but satellite bouton formation is slightly elevated.

Cdc422 third instar larvae exhibit synaptic overgrowth and an increase in bouton number in segment A3, muscle 6/7 compared to controls.

Cdc424/Cdc422 flies show normal resistance to infection with P.aeruginosa by septic injury.

Cdc422 mutants exhibit a delay in F-actin enrichment and accumulation in rhabdomeres.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

Cdc42[+], WASp[+], Cdc422, WASp1 is an enhancer of abnormal neuroanatomy phenotype of WASp1

Cdc42[+]/Cdc422 is an enhancer of abnormal neuroanatomy phenotype of Cip41

Cdc42[+], WASp[+], WASp1, Cdc422 is an enhancer of abnormal neuroanatomy phenotype of Cip41

Cdc42[+], Cip41, Cip4[+], Cdc422 is an enhancer of abnormal neuroanatomy phenotype of WASp1

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Cdc422 has axon | adult stage phenotype, enhanceable by Frl[+]/FrlEx83

Cdc422 has axon | adult stage phenotype, enhanceable by Frl[+]/FrlEx88

Cdc422 has axon | adult stage phenotype, enhanceable by Frl[+]/FrlExK62

Cdc422 has NMJ bouton phenotype, enhanceable by Cip41/Cip4[+]

Cdc422 has neuromuscular junction phenotype, enhanceable by Cip41/Cip4[+]

Cdc422 has NMJ bouton phenotype, enhanceable by Cip41/WASp[+]/WASp1/Cip4[+]

Cdc422 has neuromuscular junction phenotype, enhanceable by Cip41/WASp[+]/WASp1/Cip4[+]

Suppressed by
NOT suppressed by
Statement
Reference

Cdc422 has phenotype, non-suppressible by Merhs.PM

Enhancer of
Statement
Reference

Cdc42[+]/Cdc422 is an enhancer of NMJ bouton phenotype of Cip41

Cdc42[+]/Cdc422 is an enhancer of neuromuscular junction phenotype of Cip41

Cdc42[+], WASp[+], WASp1, Cdc422 is an enhancer of NMJ bouton phenotype of Cip41

Cdc42[+], WASp[+], WASp1, Cdc422 is an enhancer of neuromuscular junction phenotype of Cip41

Cdc42[+], Cip41, Cip4[+], Cdc422 is an enhancer of NMJ bouton phenotype of WASp1

Cdc42[+], Cip41, Cip4[+], Cdc422 is an enhancer of neuromuscular junction phenotype of WASp1

Suppressor of
Statement
Reference

Cdc42[+]/Cdc422 is a suppressor of mitotic domain 1 | embryonic cycle 14 phenotype of CycB+t10

NOT Suppressor of
Statement
Reference

Cdc42[+]/Cdc422 is a non-suppressor of aster | embryonic cycle 5 phenotype of CycB+t10

Cdc42[+]/Cdc422 is a non-suppressor of aster | embryonic cycle 6 phenotype of CycB+t10

Cdc42[+]/Cdc422 is a non-suppressor of aster | embryonic cycle 7 phenotype of CycB+t10

Cdc42[+]/Cdc422 is a non-suppressor of mitotic domain 1 | embryonic cycle 10 phenotype of CycB+t10

Additional Comments
Genetic Interactions
Statement
Reference

Presence of FrlEx83/+, FrlEx88/+ or FrlExK62/+ enhances the mushroom body axon growth defects seen in Cdc422/Y flies.

ZirBG00267/+ ; Cdc422/+ double heterozygous larvae have a reduced rate of encapsulation (37%) of eggs of the avirulent wasp strain L. boulardi G486 compared to the rate seen in either single heterozygote.

Cdc422; Cip41 double heterozygotes exhibit an increase in total bouton number, satellite bouton formation and neuromuscular junction length.

Cdc422/+; Cip41/+; WASp1/+ trans-heterozygous larvae exhibit an increase in total bouton number, neuromuscular junction length and in particular satellite bouton formation.

Cdc422 nwk1 double mutants exhibit more severe synaptic overgrowth and an increased number of boutons and satellite boutons compared to either single mutant.

Cdc422 nwk2 double mutants exhibit more severe synaptic overgrowth and an increased number of boutons and satellite boutons compared to either single mutant.

Cdc422 nwk2/nwk1 double mutants exhibit an increased number of satellite boutons in segment A2-A3, muscle 4 compared to either Cdc422 or nwk2 homozygous single mutants.

Expression of Cdc42Ubi-p63E.T:Hsap\MYC rescues the synaptic overgrowth and increased bouton and satellite bouton number phenotypes seen in nwk2 Cdc422 double mutant larvae.

The Cdc422 mutation fails to modify the cv-cM62 phenotype in the Malpighian tubules.

A Cdc422/+ background enhances the splitting wing hair phenotype of flies that express trcT453A.Scer\UAS under the control of Scer\GAL4ap-md544.

Not rescued by Merhs.PM.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 2 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
Synonyms and Secondary IDs (4)
References (15)