Allele Dmel\Cdc42²

General Information
Symbol	Dmel\Cdc42²	Species	D. melanogaster
Name		FlyBase ID	FBal0062065
Feature type	allele	Associated gene	Dmel\Cdc42

Map ( GBrowse )
Allele class	hypomorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Fehon et al., 1997)
Mutagen	ethyl methanesulfonate (Fehon et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation X:19,592,280..19,592,280 evidence=experimental reported_na_change=T311A na_change=T19592280A (Fehon et al., 1997, Genova et al., 2000)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AF153425 AAD43789

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Nucleotide substitution: T311A. Mutation in splice donor consensus site. (Genova et al., 2000) Mutation in the splice donor sequence of the small internal intron. (Fehon et al., 1997)
Cytology
Phenotypic Data
Phenotypic Class
	immune response defective \| larval stage (Howell et al., 2012, Sampson et al., 2012) lethal \| embryonic stage \| non-rescuable maternal effect (Leibfried et al., 2013) neuroanatomy defective (Nahm et al., 2010) neuroanatomy defective \| third instar larval stage (Rodal et al., 2008) size defective \| third instar larval stage (Rodal et al., 2008) viable (Fehon et al., 1997) visible (Genova et al., 2000, Fehon et al., 1997)
Phenotype Manifest In
	actin filament & rhabdomere \| somatic clone (Zelhof and Hardy, 2004) bouton (Nahm et al., 2010) embryonic/larval neuromuscular junction \| third instar larval stage (Rodal et al., 2008) neuromuscular junction (Nahm et al., 2010) NMJ bouton \| third instar larval stage (Rodal et al., 2008)
Detailed Description
	Statement Reference The hatching rate of eggs derived from homozygous females is reduced compared to wild type, regardless of the zygotic state. (Leibfried et al., 2013) Mutant larvae fail to encapsulate eggs when parasitised by the avirulent L. boulardi wasp strain G486 (no melanisation is seen). Plasmatocytes and lamellocytes fully cover the egg. (Howell et al., 2012) Homozygous larvae fail to encapsulate eggs of the avirulent wasp strain L. boulardi G486. Isolated plasmatocytes from homozygous larvae show a reduced ability to phagocytose E. coli and S. aureus in a phagocytosis assay. (Sampson et al., 2012) Cdc42[2] heterozygous larvae exhibit wild-type neuromuscular junction length and total bouton number, but satellite bouton formation is slightly elevated. (Nahm et al., 2010) Cdc42[2] third instar larvae exhibit synaptic overgrowth and an increase in bouton number in segment A3, muscle 6/7 compared to controls. (Rodal et al., 2008) Cdc42[4]/Cdc42[2] flies show normal resistance to infection with P.aeruginosa by septic injury. (Avet-Rochex et al., 2007) Cdc42² mutants exhibit a delay in F-actin enrichment and accumulation in rhabdomeres. (Zelhof and Hardy, 2004)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Cdc42² has neuroanatomy defective \| third instar larval stage phenotype, enhanceable by nwk¹ (Rodal et al., 2008) Cdc42² has neuroanatomy defective \| third instar larval stage phenotype, enhanceable by nwk¹/nwk² (Rodal et al., 2008) Cdc42² has neuroanatomy defective \| third instar larval stage phenotype, enhanceable by nwk² (Rodal et al., 2008) Cdc42² has neuroanatomy defective phenotype, enhanceable by Cip4¹/Cip4[+] (Nahm et al., 2010) Cdc42² has neuroanatomy defective phenotype, enhanceable by Cip4¹/WASp[+]/WASp¹/Cip4[+] (Nahm et al., 2010) Cdc42² has size defective \| third instar larval stage phenotype, enhanceable by nwk¹ (Rodal et al., 2008) Cdc42² has size defective \| third instar larval stage phenotype, enhanceable by nwk² (Rodal et al., 2008)
Suppressed by
Statement Reference Cdc42², nwk² has size defective \| third instar larval stage phenotype, suppressible by Cdc42^{Ubi-p63E.T:Hsap\MYC} (Rodal et al., 2008)
Enhancer of
Statement Reference Cdc42[+], Cip4¹, Cip4[+], Cdc42² is an enhancer of neuroanatomy defective phenotype of WASp¹ (Nahm et al., 2010) Cdc42[+], WASp[+], Cdc42², WASp¹ is an enhancer of neuroanatomy defective phenotype of WASp¹ (Nahm et al., 2010) Cdc42[+], WASp[+], WASp¹, Cdc42² is an enhancer of neuroanatomy defective phenotype of Cip4¹ (Nahm et al., 2010) Cdc42[+]/Cdc42² is an enhancer of neuroanatomy defective phenotype of Cip4¹ (Nahm et al., 2010) Cdc42² is an enhancer of neuroanatomy defective \| third instar larval stage phenotype of nwk¹ (Rodal et al., 2008) Cdc42² is an enhancer of size defective \| third instar larval stage phenotype of nwk¹ (Rodal et al., 2008) Cdc42² is an enhancer of size defective \| third instar larval stage phenotype of nwk² (Rodal et al., 2008)
Other
Statement Reference Cdc42[+]/Cdc42², Zir^BG00267 has immune response defective \| dominant \| larval stage phenotype (Sampson et al., 2012)
Phenotype Manifest In
Enhanced by
Statement Reference Cdc42² has embryonic/larval neuromuscular junction \| third instar larval stage phenotype, enhanceable by nwk¹ (Rodal et al., 2008) Cdc42² has embryonic/larval neuromuscular junction \| third instar larval stage phenotype, enhanceable by nwk¹/nwk² (Rodal et al., 2008) Cdc42² has embryonic/larval neuromuscular junction \| third instar larval stage phenotype, enhanceable by nwk² (Rodal et al., 2008) Cdc42² has neuromuscular junction phenotype, enhanceable by Cip4¹/Cip4[+] (Nahm et al., 2010) Cdc42² has neuromuscular junction phenotype, enhanceable by Cip4¹/WASp[+]/WASp¹/Cip4[+] (Nahm et al., 2010) Cdc42² has NMJ bouton \| third instar larval stage phenotype, enhanceable by nwk¹ (Rodal et al., 2008) Cdc42² has NMJ bouton \| third instar larval stage phenotype, enhanceable by nwk¹/nwk² (Rodal et al., 2008) Cdc42² has NMJ bouton \| third instar larval stage phenotype, enhanceable by nwk² (Rodal et al., 2008) Cdc42² has NMJ bouton phenotype, enhanceable by Cip4¹/Cip4[+] (Nahm et al., 2010) Cdc42² has NMJ bouton phenotype, enhanceable by Cip4¹/WASp[+]/WASp¹/Cip4[+] (Nahm et al., 2010)
Suppressed by
Statement Reference Cdc42², nwk² has embryonic/larval neuromuscular junction \| third instar larval stage phenotype, suppressible by Cdc42^{Ubi-p63E.T:Hsap\MYC} (Rodal et al., 2008) Cdc42², nwk² has NMJ bouton \| third instar larval stage phenotype, suppressible by Cdc42^{Ubi-p63E.T:Hsap\MYC} (Rodal et al., 2008)
NOT suppressed by
Statement Reference Cdc42² has phenotype, non-suppressible by Mer^hs.PM (Fehon et al., 1997)
Enhancer of
Statement Reference Cdc42[+], Cip4¹, Cip4[+], Cdc42² is an enhancer of neuromuscular junction phenotype of WASp¹ (Nahm et al., 2010) Cdc42[+], Cip4¹, Cip4[+], Cdc42² is an enhancer of NMJ bouton phenotype of WASp¹ (Nahm et al., 2010) Cdc42[+], WASp[+], WASp¹, Cdc42² is an enhancer of neuromuscular junction phenotype of Cip4¹ (Nahm et al., 2010) Cdc42[+], WASp[+], WASp¹, Cdc42² is an enhancer of NMJ bouton phenotype of Cip4¹ (Nahm et al., 2010) Cdc42[+]/Cdc42² is an enhancer of neuromuscular junction phenotype of Cip4¹ (Nahm et al., 2010) Cdc42[+]/Cdc42² is an enhancer of NMJ bouton phenotype of Cip4¹ (Nahm et al., 2010) Cdc42[+]/Cdc42² is an enhancer of wing hair \| supernumerary phenotype of Scer\GAL4^ap-md544, trc^{T453A.Scer\UAS} (He et al., 2005) Cdc42² is an enhancer of embryonic/larval neuromuscular junction \| third instar larval stage phenotype of nwk¹ (Rodal et al., 2008) Cdc42² is an enhancer of embryonic/larval neuromuscular junction \| third instar larval stage phenotype of nwk² (Rodal et al., 2008) Cdc42² is an enhancer of NMJ bouton \| third instar larval stage phenotype of nwk² (Rodal et al., 2008)
Suppressor of
Statement Reference Cdc42[+]/Cdc42² is a suppressor of cycle 14 embryo phenotype of CycB^+t10 (Ji et al., 2002) Cdc42² is a suppressor of NMJ bouton \| third instar larval stage phenotype of nwk¹ (Rodal et al., 2008)
NOT Suppressor of
Statement Reference Cdc42[+]/Cdc42² is a non-suppressor of aster \| embryonic cycle 5 phenotype of CycB^+t10 (Ji et al., 2002) Cdc42[+]/Cdc42² is a non-suppressor of aster \| embryonic cycle 6 phenotype of CycB^+t10 (Ji et al., 2002) Cdc42[+]/Cdc42² is a non-suppressor of aster \| embryonic cycle 7 phenotype of CycB^+t10 (Ji et al., 2002) Cdc42[+]/Cdc42² is a non-suppressor of cycle 10 embryo phenotype of CycB^+t10 (Ji et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference Zir[BG00267]/+ ; Cdc42[2]/+ double heterozygous larvae have a reduced rate of encapsulation (37%) of eggs of the avirulent wasp strain L. boulardi G486 compared to the rate seen in either single heterozygote. (Sampson et al., 2012) Cdc42[2]; Cip4[1] double heterozygotes exhibit an increase in total bouton number, satellite bouton formation and neuromuscular junction length. Cdc42[2]/+; Cip4[1]/+; WASp[1]/+ trans-heterozygous larvae exhibit an increase in total bouton number, neuromuscular junction length and in particular satellite bouton formation. (Nahm et al., 2010) Cdc42[2] nwk[1] double mutants exhibit more severe synaptic overgrowth and an increased number of boutons and satellite boutons compared to either single mutant. Cdc42[2] nwk[2] double mutants exhibit more severe synaptic overgrowth and an increased number of boutons and satellite boutons compared to either single mutant. Cdc42[2] nwk[2]/nwk[1] double mutants exhibit an increased number of satellite boutons in segment A2-A3, muscle 4 compared to either Cdc42[2] or nwk[2] homozygous single mutants. Expression of Cdc42[Ubi-p63E.T:Hsap\MYC] rescues the synaptic overgrowth and increased bouton and satellite bouton number phenotypes seen in nwk[2] Cdc42[2] double mutant larvae. (Rodal et al., 2008) The Cdc42² mutation fails to modify the cv-c^M62 phenotype in the Malpighian tubules. (Denholm et al., 2005) A Cdc42²/+ background enhances the splitting wing hair phenotype of flies that express trc^{T453A.Scer\UAS} under the control of Scer\GAL4^ap-md544. (He et al., 2005) Not rescued by Mer^hs.PM. (Fehon et al., 1997)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Cdc42² is rescued by Cdc42^Ubi-p63E.PF (Fehon et al., 1997)
Comments
Stocks ( 1 )
Bloomington	9105 y¹ w^* Cdc42² P{neoFRT}19A
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Cdc42 2 (Howell et al., 2012) cdc42² (Denholm et al., 2005, Avet-Rochex et al., 2007, Nahm et al., 2010, Rodal et al., 2008) Cdc42² (Sampson et al., 2012, Frank et al., 2009, Leibfried et al., 2013) Dcdc42² (He et al., 2005)
Name Synonym
Secondary FlyBase IDs

References ( 13 )
Research paper	Leibfried et al., 2013, Development 140(2): 362--371 A Cdc42-regulated actin cytoskeleton mediates Drosophila oocyte polarization. [FBrf0220348] Howell et al., 2012, Immunogenetics 64(2): 155--161 A directed miniscreen for genes involved in the Drosophila anti-parasitoid immune response. [FBrf0217123] Sampson et al., 2012, Dev. Comp. Immunol. 38(1): 160--168 The RhoGEF Zizimin-related acts in the Drosophila cellular immune response via the Rho GTPases Rac2 and Cdc42. [FBrf0219019] Nahm et al., 2010, J. Neurosci. 30(24): 8138--8150 dCIP4 (Drosophila Cdc42-interacting protein 4) restrains synaptic growth by inhibiting the secretion of the retrograde Glass bottom boat signal. [FBrf0211075] Frank et al., 2009, Neuron 61(4): 556--569 A presynaptic homeostatic signaling system composed of the Eph receptor, ephexin, Cdc42, and CaV2.1 calcium channels. [FBrf0207531] Rodal et al., 2008, J. Neurosci. 28(33): 8316--8325 Nervous wreck and Cdc42 cooperate to regulate endocytic actin assembly during synaptic growth. [FBrf0205746] Avet-Rochex et al., 2007, Genes Cells 12(10): 1193--1204 Rac2 is a major actor of Drosophila resistance to Pseudomonas aeruginosa acting in phagocytic cells. [FBrf0200813] Denholm et al., 2005, Development 132(10): 2389--2400 crossveinless-c is a RhoGAP required for actin reorganisation during morphogenesis. [FBrf0187455] He et al., 2005, Mol. Biol. Cell 16(2): 689--700 The tricornered Ser/Thr protein kinase is regulated by phosphorylation and interacts with furry during Drosophila wing hair development. [FBrf0184141] Zelhof and Hardy, 2004, J. Cell Biol. 164(3): 417--426 WASp is required for the correct temporal morphogenesis of rhabdomere microvilli. [FBrf0167774] Ji et al., 2002, Genetics 162(3): 1179--1195 A genetic screen for suppressors and enhancers of the Drosophila cdk1-cyclin B identifies maternal factors that regulate microtubule and microfilament stability. [FBrf0155492] Genova et al., 2000, Dev. Biol. 221(1): 181--194 Functional analysis of cdc42 in actin filament assembly, epithelial morphogenesis, and cell signaling during Drosophila development. [FBrf0127090] Fehon et al., 1997, Genetics 146(1): 245--252 Isolation of mutations in the Drosophila homologues of the human Neurofibromatosis 2 and yeast CDC42 genes using a simple and efficient reverse-genetic method. [FBrf0093443]

Allele Dmel\Cdc422

Allele Dmel\Cdc42²