A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Allele BacA\p35Scer\UAS.cHa

General Information
SymbolBacA\p35Scer\UAS.cHaSpeciesN. Autographa californica nucleopolyhedrovirus
NameSaccharomyces cerevisiae UAS construct a of HayFlyBase IDFBal0062158
Feature typealleleCreated / Updated2006-08-20/2006-08-20
Associated geneBacA\p35
Allele class
Mutagenin vitro construct | regulatory fusion
hide Nature of the Allele
Allele class
Mutagen
in vitro construct | regulatory fusion
(Zhou et al., 1997, Neufeld et al., 1998)
Mapped Features and Mutations
Type
Symbol & Location
Additional Notes
References
 
 
 
 
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Progenitor genotype
      Nature of the lesion
      Statement
      Reference
      Construct: Expression of BacA\p35 is influenced by Scer\UAS Scer\GAL4 binding sites.
      (Zhou et al., 1997)
      Assay mode
      Drosophila cell culture
      (Igaki et al., 2000, Igaki et al., 2002)
      In transgenic Drosophila (allele of foreign species in genome of drosophilid)
      (Zhou et al., 1997, Mirkovic et al., 2002, Saucedo et al., 2003, Neufeld et al., 1998)
      Carried in construct
      P{UAS-p35.H}
      (Wood et al., 2006, Berry and Baehrecke, 2007, Li and Baker, 2007, Negre et al., 2003, Asha et al., 2003, Olson et al., 2003, Moreno et al., 2002, Lundell et al., 2003, Satoh and Ready, 2005, Kanuka et al., 2005, Sato and Tomlinson, 2007, Kanuka et al., 1999, Moser and Campbell, 2005, Jang et al., 2003, Renn et al., 1999, Karim and Rubin, 1998, Coelho et al., 2005, Marenda et al., 2006, Perrin et al., 2003, Krupp et al., 2005, Kanuka et al., 2005, Arama et al., 2003, Shigenaga et al., 1997, Jiao et al., 2001, Saucedo et al., 2003, Kramer et al., 2003, Mueller et al., 2005, Williams and Truman, 2005, Wing et al., 2002, Yoshida et al., 2001, Martin-Castellanos and Edgar, 2002, Hidalgo et al., 2001, Watts et al., 2003, Georgel et al., 2001, Cenci and Gould, 2005, Xue and Noll, 2002, Li et al., 2003, Frank et al., 2002, Perez-Garijo et al., 2005, Milan et al., 2001, Giesen et al., 2003, Otsuki et al., 2004, Hsu et al., 2007, Sprecher et al., 2006, Milan and Cohen, 2003, Moreno et al., 2002, Besse and Pret, 2003, Bloor and Kiehart, 2002, Milan et al., 2002, Noureddine et al., 2002, Mirkovic et al., 2002, Huh et al., 2004, Geisbrecht and Montell, 2004, Sen et al., 2004, Hipfner and Cohen, 2003, Bauer et al., 2004, Tseng and Hariharan, 2002, Lee and Park, 2004, Prober and Edgar, 2002, Ryoo et al., 2002, Yoo et al., 2002, Baena-Lopez and Garcia-Bellido, 2003, Kango-Singh et al., 2002, Fichelson and Gho, 2003, Niwa et al., 2004, Mergliano and Minden, 2003, Brumby and Richardson, 2003, Igaki et al., 2002, Lin et al., 2004, Kimura et al., 2004, Cox et al., 2000, del Alamo Rodriguez et al., 2004, Prober and Edgar, 2000, Moreno and Basler, 2004, Giraldez and Cohen, 2003, de la Cova et al., 2004, Lee and Baehrecke, 2001, Wolf and Schuh, 2000, Blaumueller and Mlodzik, 2000, Huang et al., 1999, Gao et al., 2000, Namba and Minden, 1999, Wing et al., 2001, Migeon et al., 1999, Gibson and Perrimon, 2005, Grewal et al., 2005, Cullen and McCall, 2004, Macias et al., 2004, Aldaz et al., 2005, Griffiths and Hidalgo, 2004, Jiang et al., 1997, Sotillos and Campuzano, 2000, Sustar and Schubiger, 2005, Takata et al., 2004, Miguel-Aliaga and Thor, 2004, Perez-Garijo et al., 2004, Martin et al., 2004, Shandala et al., 2004, Sykes et al., 2004, Miron et al., 2001, Mihaly et al., 2001, Wing et al., 2001, Neufeld et al., 1998, Tapon et al., 2001, Pielage et al., 2003, Warrick et al., 1998, Johnston and Sanders, 2003, Zhou et al., 1997, Pallavi and Shashidhara, 2003, Chandrasekaran and Beckendorf, 2003, Aldaz et al., 2003, DeFalco et al., 2003, Johnston et al., 1999, Rogulja and Irvine, 2005, Almeida and Bray, 2005, Jones et al., 2006, Wichmann et al., 2006, Choi et al., 2006, Parker, 2006, Huelsmann et al., 2006, Bauer et al., 2005, Bauer et al., 2005, McEwen and Peifer, 2005, Rives et al., 2006, Jafar-Nejad et al., 2006, Wells et al., 2006, Nolo et al., 2006, Baena-Lopez and Garcia-Bellido, 2006, Ryoo et al., 2004, Cela and Llimargas, 2006, Crickmore and Mann, 2006, Oshima et al., 2006, Oshima, 2006, Gregory et al., 2007, Herranz et al., 2006, Han et al., 2006, Chartier et al., 2006, Zhang et al., 2006, Zhai et al., 2006, Legent et al., 2006, Bruckner et al., 2004, Singh et al., 2006, Liu et al., 2005, Huh et al., 2007, Grosskortenhaus et al., 2006, Hyun et al., 2006, Uhlirova and Bohmann, 2006, Renault et al., 2004, Bello et al., 2007, Sugiyama et al., 2007, Lichtneckert et al., 2007, Mathieu et al., 2007, Assa-Kunik et al., 2007, Karres et al., 2007, Kugler and Nagel, 2007)
      Cytology
      hide Phenotypic Data
      hide Phenotypic Class
      viable, with Scer\GAL4en-e16E
      (Perez-Garijo et al., 2004)
      viable, with Scer\GAL4ap-md544
      (Perez-Garijo et al., 2004)
      viable, with Scer\GAL4nub-AC-62
      (Perez-Garijo et al., 2004)
      viable, with Scer\GAL4hh-Gal4
      (Perez-Garijo et al., 2004)
      visible, with Scer\GAL4sca-537.4
      (Kanuka et al., 1999)
      visible, with Scer\GAL4dpp.blk1
      (Kanuka et al., 2005, Kanuka et al., 1999)
      visible, with Scer\GAL4ptc-559.1
      (Kanuka et al., 1999)
      visible, with Scer\GAL4pnr-MD237
      (Ryoo et al., 2002)
      visible, with Scer\GAL4sca-P309
      (Kanuka et al., 2005)
      increased cell number | somatic clone, with Scer\GAL4tub
      (Perez-Garijo et al., 2005)
      male sterile, with Scer\GAL4Hsp83.PA
      (Arama et al., 2003)
      female fertile, with Scer\GAL4how-24B, Scer\GAL4twi.PB
      (DeFalco et al., 2003)
      cell death defective | somatic clone, with Scer\GAL4tub
      (Perez-Garijo et al., 2005)
      cell death defective, with Scer\GAL469B
      (Mirkovic et al., 2002)
      cell death defective, with Scer\GAL4dpp.blk1
      (Karim and Rubin, 1998)
      cell death decrease | pupal stage, with Scer\GAL4GMR.PF
      (Lin et al., 2004)
      cell death decrease, with Scer\GAL4fkh.PH
      (Lee and Baehrecke, 2001)
      cell death decrease, with Scer\GAL4Ddc.PL
      (Sykes et al., 2004)
      cell death decrease, with Scer\GAL4nub-AC-62
      (Martin et al., 2004)
      cell death decrease, with Scer\GAL4Vap.P0201
      (Miguel-Aliaga and Thor, 2004)
      cell death decrease, with Scer\GAL4Crz
      (Choi et al., 2006)
      hide Phenotype Manifest In
      male terminalia, with Scer\GAL4en-e16E
      (Macias et al., 2004)
      adult external abdomen | dorsal, with Scer\GAL4en-e16E
      (Macias et al., 2004)
      wing vein, with Scer\GAL4en-e16E
      (Marenda et al., 2006)
      male genitalia, with Scer\GAL4en-e16E
      (McEwen and Peifer, 2005)
      male terminalia, with Scer\GAL4dpp.blk1
      (Macias et al., 2004)
      adult external abdomen | dorsal, with Scer\GAL4dpp.blk1
      (Macias et al., 2004)
      macrochaeta | supernumerary, with Scer\GAL4dpp.blk1
      (Kanuka et al., 2005)
      scutellar bristle | ectopic, with Scer\GAL4dpp.blk1
      (Kanuka et al., 1999)
      dopamine neuron | supernumerary, with Scer\GAL4Ddc.PL
      (Sykes et al., 2004)
      dorsal mesothoracic disc | somatic clone, with Scer\GAL4tub
      (Perez-Garijo et al., 2005)
      dorsal mesothoracic disc, with Scer\GAL4C10
      (de la Cova et al., 2004)
      gonadal sheath proper primordium | female, with Scer\GAL4how-24B, Scer\GAL4twi.PB
      (DeFalco et al., 2003)
      macrochaeta | ectopic & scutellum, with Scer\GAL4pnr-MD237
      (Ryoo et al., 2002)
      eye disc, with Scer\GAL4GMR.PF
      (Kango-Singh et al., 2002)
      eye, with Scer\GAL4GMR.PF
      (Moreno et al., 2002)
      ommatidium, with Scer\GAL4GMR.PF
      (Moreno et al., 2002)
      eye photoreceptor cell & pupa | ectopic, with Scer\GAL4GMR.PF
      (Lin et al., 2004)
      ommatidium & pupa | ectopic, with Scer\GAL4GMR.PF
      (Lin et al., 2004)
      ommatidium & adult | ectopic, with Scer\GAL4GMR.PF
      (Lin et al., 2004)
      salivary gland, with Scer\GAL4fkh.PH
      (Lee and Baehrecke, 2001)
      larval salivary gland, with Scer\GAL4fkh.PH
      (Berry and Baehrecke, 2007)
      glial cell | ectopic & antennal disc, with Scer\GAL4Dll-981
      (Sen et al., 2004)
      macrochaeta | supernumerary, with Scer\GAL4sca-P309
      (Kanuka et al., 2005)
      macrochaeta | supernumerary & larva, with Scer\GAL4hs.PB
      (Kanuka et al., 2005)
      glial cell | ectopic | conditional ts & antennal disc, with Scer\GAL4hs.PB
      (Sen et al., 2004)
      larval salivary gland, with Scer\GAL4D59
      (Jiang et al., 1997)
      dMP2 neuron & stage 17 embryo, with Scer\GAL4Vap.P0201
      (Miguel-Aliaga and Thor, 2004)
      polar follicle cell | supernumerary, with Scer\GAL4neur-P72
      (Besse and Pret, 2003)
      polar follicle cell | somatic clone | supernumerary, with Scer\GAL4Act5C.PP
      (Besse and Pret, 2003)
      follicle cell | somatic clone, with Scer\GAL4Act5C.PP
      (Besse and Pret, 2003)
      egg chamber | somatic clone, with Scer\GAL4Act5C.PP
      (Besse and Pret, 2003)
      border follicle cell | somatic clone, with Scer\GAL4Act5C.PP
      (Besse and Pret, 2003)
      border follicle cell | somatic clone | supernumerary, with Scer\GAL4Act5C.PP
      (Besse and Pret, 2003)
      scutellar bristle | ectopic, with Scer\GAL4ptc-559.1
      (Kanuka et al., 1999)
      macrochaeta | supernumerary, with Scer\GAL4ptc-559.1
      (McEwen and Peifer, 2005)
      scutellar bristle | ectopic, with Scer\GAL4sca-537.4
      (Kanuka et al., 1999)
      cystic bulge, with Scer\GAL4Hsp83.PA
      (Arama et al., 2003)
      embryonic head, with Scer\GAL469B
      (Mirkovic et al., 2002)
      external sensory organ & embryo | ectopic, with Scer\GAL4arm.PS
      (Orgogozo et al., 2002)
      wing vein L5, with Scer\GAL4hh-Gal4
      (Perez-Garijo et al., 2004)
      glial cell | ectopic & antennal disc, with Scer\GAL4lz-gal4
      (Sen et al., 2004)
      glial cell | ectopic & antennal disc, with Scer\GAL4pros.PMG
      (Sen et al., 2004)
      midline glial cell | ectopic, with Scer\GAL4sim.PS
      (Giesen et al., 2003)
      mitotic domain 20, with Scer\GAL4cCa.T:Hsim\VP16
      (Namba and Minden, 1999)
      neuron & ventral nerve cord, with Scer\GAL4Crz
      (Choi et al., 2006)
      larval epidermis | somatic clone, with Scer\GAL4Act5C.PI
      (Ninov et al., 2007)
      histoblast nest, with Scer\GAL4Act5C.PI
      (Ninov et al., 2007)
      adult abdomen, with Scer\GAL4Act5C.PI
      (Ninov et al., 2007)
      interfollicle cell, with Scer\GAL4109-53
      (Assa-Kunik et al., 2007)
      hide Detailed Description
      Statement
      Reference
      Scer\GAL4D59-mediated expression causes failure of salivary gland histolysis in 15 hour prepupae, the salivary glands can still be detected 22 hours after puparium formation. The salivary glands at this stage are abnormal in morphology and appear to be partially degraded.
      (Jiang et al., 1997)
      When expression is driven by Scer\GAL4cCa.T:Hsim\VP16 in mitotic domain 20 at gastrulation an expansion of the dorsal surface epithelium occurs, though cell death still occurs. Defects are mild compared to those of Df(3L)H99 embryos.
      (Namba and Minden, 1999)
      Expression of BacA\p35Scer\UAS.cHa in the scutellum under the control of Scer\GAL4ptc-559.1, Scer\GAL4dpp.blk1 or Scer\GAL4sca-537.4 results in the formation of ectopic bristles.
      (Kanuka et al., 1999)
      When expression is driven by Scer\GAL4fkh.PH salivary glands survive the cell death that would have been their normal fate. DNA fragmentation is suppressed, though they have progressed to a late stage of autophagy (i.e. vacuoles and associated plasma membranes are often absent from the cytosol).
      (Lee and Baehrecke, 2001)
      Embryos expressing BacA\p35 using Scer\GAL469B with BacA\p35Scer\UAS.cHa have severe head defects, while denticle patterning appears fairly normal. Cell death is completely blocked in these embryos.
      (Mirkovic et al., 2002)
      BacA\p35Scer\UAS.cHa; Scer\GAL4C-765 flies have no discernible wing phenotype. BacA\p35Scer\UAS.cHa; Scer\GAL4GMR.PF flies have subtle ommatidial disorganisation.
      (Moreno et al., 2002)
      In BacA\p35Scer\UAS.cHa; Scer\GAL4arm.PS embryos, an ectopic external sensory organ forms near the abdominal ventral multidendritic neuron a1 neuron in many abdominal segments.
      (Orgogozo et al., 2002)
      Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4GMR.PF results in extra interommatidial cells in the eye disc.
      (Kango-Singh et al., 2002)
      Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4neur-P72 produces supernumerary polar cells in advanced egg chambers. Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PP in clones in the egg chamber results in the production of extra polar cells. No obvious defects are seen in the early development of these egg chambers. 80% of stage 9 egg chambers expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PP have round anterior follicle cells, a constant thickness of epithelial cells along the anterior-posterior axis and a homogeneous distribution of anterior follicle cell nuclei (in contrast to wild-type stage 9 egg chambers which have elongated anterior follicle cells, thinning of the epithelium at the anterior end and a reduction in density of follicle cell nuclei at the anterior pole). 91% of stage 10 egg chambers expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PP have defects in delamination and migration of border cells; the border cells are either still stuck at the anterior pole or are only partway between the anterior pole and the oocyte. These clusters often contain supernumerary border cells.
      (Besse and Pret, 2003)
      Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Cg.PA does not cause an appreciable increase in hemocyte number in third instar larvae.
      (Asha et al., 2003)
      When BacA\p35Scer\UAS.cHa is expressed under the control of Scer\GAL4sim.PS an increase in the number of midline glial cells is seen. About 6 cells are seen per neuromere.
      (Giesen et al., 2003)
      Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4pnr-MD237 results in extra macrochaetae in the scutellum in 30% of animals.
      (Ryoo et al., 2002)
      Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4neur-P72 suppresses the fragmentation of the glial cell which is normally seen in the thoracic microchaete lineage in the developing notum; at 24 hours after puparium formation (APF), 86% of the clusters contain glial cells in these animals (compared to 8% of clusters in control animals). The ectopically surviving glial cells are tightly associated with axonal processes in 72% of sensory organs studied at 24 hours APF (in 55% of cases the glial cells are located at the growth cone, while in the remaining 17.5% they are associated with the axon), while in 27.5% of the sensory organs studied the glial cells remain within the proximity of the cluster. At 27 hours APF, the axonal network of the notum and its orientation shows no major difference between wild-type pupae and pupae expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4neur-P72. Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4neur-P72 promotes precocious axonogenesis; axonal growth cones of notal microchaetae are observed 23.5 hours APF in these flies, compared to 25 hours APF in wild type. The cleaning reflex is normal in adults expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4neur-P72.
      (Fichelson and Gho, 2003)
      When driven by Scer\GAL4OK107 or Scer\GAL4Tab2-201Y no defects in axon pruning or the mushroom body are seen.
      (Watts et al., 2003)
      When BacA\p35Scer\UAS.cHa is driven by Scer\GAL4αTub84B.PL in scrib1 clones in the eye, the amount of mutant tissue surviving to adulthood increases compared with scrib1 clones alone.
      (Brumby and Richardson, 2003)
      When BacA\p35Scer\UAS.cHa is driven by Scer\GAL4Dll-981 in the antennal disc >300 glial cells are formed as opposed to the ~100 in wild-type. The organisation of sensory afferent fascicles from the antenna is unaffected. When BacA\p35Scer\UAS.cHa is driven by Scer\GAL4lz-gal4 in the antennal disc a significant increase in glial cell number is seen. When BacA\p35Scer\UAS.cHa is driven by Scer\GAL4pros.PMG in the antennal disc a significant increase in glial cell number is seen. When BacA\p35Scer\UAS.cHa is driven by Scer\GAL4ato.3.6 in the antennal disc no alteration is seen in the number of glial cells. When BacA\p35Scer\UAS.cHa is driven by Scer\GAL4hs.PB and heat pulsed up to 6-8 hours after puparium formation (AFP) no significant alterations in glial number are seen. While pulsing during the 14 to 18 AFP period results in a very large increase in glial cell numbers.
      (Sen et al., 2004)
      When BacA\p35Scer\UAS.cHa is driven in the wing disc by Scer\GAL4C10, no effect on the average size of the wing disc is seen. However significantly variability is in the size of the wing disc.
      (de la Cova et al., 2004)
      BacA\p35Scer\UAS.cHa; Scer\GAL4Hsp83.PA males are infertile (males carrying either construct alone are not). Although the initial assembly of the "individualisation complex" at the head of the cyst appears normal, there is a failure of cytoplasm to collect into a normal cystic bulge.
      (Arama et al., 2003)
      In the wings of BacA\p35Scer\UAS.cHa; Scer\GAL4en-e16E flies many cells persist between the dorsal and ventral cuticular sheets.
      (Kimura et al., 2004)
      Apoptosis is eliminated and extra photoreceptor clusters are formed at the edge of the developing eye in BacA\p35Scer\UAS.cHa; Scer\GAL4GMR.PF animals 42 hours after puparium formation. These clusters survive to form ommatidia in adult flies, but are often incomplete.
      (Lin et al., 2004)
      Cell proliferation is not increased in the posterior compartment of wing discs expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en-e16E. Wings appear normal in adults expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en-e16E.
      (Huh et al., 2004)
      Expression of BacA\p35Scer\UAS.cHa, driven by Scer\GAL4Ddc.PL, results in a statistically significant increase in the number of both serotonergic and dopaminergic neurons after metamorphosis.
      (Sykes et al., 2004)
      In stage 16 female gonads, where expression of BacA\p35Scer\UAS.cHa is driven by both Scer\GAL4how-24B and Scer\GAL4twi.PB, these gonads appear masculinized; male-specific somatic gonadal precursors persist and join the posterior of female gonads, as in wild-type male embryos. The presence of such cells does not appear to affect ovary formation or oogenesis, since embryos develop into fertile adult females.
      (DeFalco et al., 2003)
      BacA\p35Scer\UAS.cHa; Scer\GAL4nub-AC-62 suppresses basal levels of apoptosis in the wing pouch.
      (Martin et al., 2004)
      Flies expressing BacA\p35Scer\UAS.cHa under the control of one of Scer\GAL4nub-AC-62, Scer\GAL4ap-md544, Scer\GAL4hh-Gal4 or Scer\GAL4en-e16E have virtually normal wings. Wing vein L5 is slightly shortened in animals expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4hh-Gal4. Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4ap-md544 prevents wing death in the dorsal, but not the ventral, compartment of the wing disc. Animals expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4hh-Gal4 which have been irradiated during the first larval instar show defects in the wing disc; the posterior compartment is abnormal in shape and increased in size. The few adults that do hatch differentiate wings with very abnormal and overgrown posterior compartments. Wing discs of animals expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4hh-Gal4 which have been irradiated during the first or second larval instar show an increase in the ratio of the size of the posterior compartment/anterior compartment compared to control discs and show greater BrdU incorporation in the posterior compartment after irradiation. In addition, cells in the anterior compartment close to the anterior-posterior (AP) border also show an increase in BrdU incorporation. The AP border becomes highly irregular in these irradiated discs and cells of posterior provenance often penetrate into the anterior compartment. These invading posterior cells do not readily mix with anterior ones, but tend to stay in separate groups. Clones in the wing disc expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PI which have been induced during the first or second larval instar and then irradiated 24 hours later, show heterogeneous incorporation of BrdU inside the clone. There is often an increase of BrdU levels in the vicinity of these clones.
      (Perez-Garijo et al., 2004)
      Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Vap.P0201 efficiently suppresses the cell death that is normally seen in anterior dMP2 neurons in late stage embryos, such that these neurons are present in late stage embryos.
      (Miguel-Aliaga and Thor, 2004)
      Males show rotated terminalia and a gap in the dorsal midline.
      (Macias et al., 2004)
      X-ray irradiated wing disc clones expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub contain a mixture of two types of cell: `live' cells, which have not initiated apoptosis due to the irradiation, and `undead' cells (between 20 and 70% of the clone), which have initiated apoptosis, but have failed to complete programmed cell death. No such cells persist outside the clones in which BacA\p35Scer\UAS.cHa is expressed. The `live' cells in the X-ray irradiated clones display increased proliferation, while the `undead' cells exhibit an abnormally slow rate of proliferation. X-ray irradiated wing disc clones expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub are also associated with local enhancements of growth, with approximately 43% of wing discs exhibiting local deformations and small outpouchings of extra tissues associated with the clones.
      (Perez-Garijo et al., 2005)
      Expression of BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4en-e16E leads to vein loss in the adult wing.
      (Marenda et al., 2006)
      Expression of BacA\p35Scer\UAS.cHa in the posterior of the wing disc, driven by Scer\GAL4en-e16E, has little effect on the patterning of adult wings.
      (Moser and Campbell, 2005)
      Inhibition of caspases in the scutellum, achieved by expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4sca-P309, results in the formation of ectopic macrochaetae on this body part in 39% of cases. Ectopic macrochaetae also appear in 23% of cases when BacA\p35Scer\UAS.cHa is expressed under the control of Scer\GAL4dpp.blk1. In contrast, the ectopic expression of BacA\p35Scer\UAS.cHa in SOP cells, driven by Scer\GAL4neur-GAL4-A101, does not result in extra macrochaetae. No significant increase in macrochaetae number is seen when BacA\p35Scer\UAS.cHa is expressed under the control of Scer\GAL4hs.PB following a heat shock at late third-instar (6-12 h APF), which is during SOP cell formation, or after it (24-30 h APF). At the stage prior to the appearance SOP cells, however, BacA\p35Scer\UAS.cHa expression leads to extra macrochaetae formation.
      (Kanuka et al., 2005)
      Expression of BacA\p35[Scer\UAS.cHa] under the control of Scer\GAL4[Crz] rescues apoptosis of Crz-expressing neurons in the ventral nerve cord during pupation.
      (Choi et al., 2006)
      Expression of BacA\p35[Scer\UAS.cHa], under the control of Scer\GAL4[elav-C155], does not increase lifespan in flies.
      (Bauer et al., 2005)
      Expression of BacA\p35[Scer\UAS.cHa], under the control of Scer\GAL4[en-e16E], results in misorientation of male external genitalia, while Scer\GAL4[ptc-559.1]-driven expression leads to an increased number of bristles. Over 96% of wings in Scer\GAL4[en-e16E]>BacA\p35[Scer\UAS.cHa] flies appear normal.
      (McEwen and Peifer, 2005)
      Expression of BacA\p35[Scer\UAS.cHa] under the control of Scer\GAL4[Act5C.PI] does not affect S2 cell morphology.
      (Oshima et al., 2006)
      Flies expressing BacA\p35[Scer\UAS.cHa] under the control of Scer\GAL4[Mhc.PW] show normal wing positions.
      (Chartier et al., 2006)
      Expression of BacA\p35[Scer\UAS.cHa] under the control of Scer\GAL4[ey.PH] in wild-type eyes does not affect eye size.
      (Singh et al., 2006)
      Expression of BacA\p35[Scer\UAS.cHa] under the control of Scer\GAL4[dpp.blk1] in the wing disc has no effect on cell morphology.
      (Uhlirova and Bohmann, 2006)
      Blocking apoptosis in larval epidermal cells by clonal expression of BacA\p35[Scer\UAS.cHa], under the control of Scer\GAL4[Act5C.PI], results in impaired extrusion of these cells from the epidermis, and failure of haemocyte recruitment and the engulfment process. The few larval epidermal cells that are able to undergo extrusion remain as viable cells under the epithelial layer. Concomitantly, there is a significant decrease in the progression of histoblast nest spreading and in the average number of histoblasts. Some of these animals survive to pharate adults, but survivors show abdominal cuticle clefts with many larval epidermal cells still present.
      (Ninov et al., 2007)
      Expression of BacA\p35[Scer\UAS.cHa] under the control of Scer\GAL4[109-53] results in an abnormal stalk between egg chambers. The stalk contains more cells than normal and the cells are not properly arranged into a one-cell wide stalk.
      (Assa-Kunik et al., 2007)
      At 24 hours after puparium formation, animals expressing BacA\p35[Scer\UAS.cHa] under the control of Scer\GAL4[fkh.PH] still contain salivary gland tissue, in contrast to wild-type animals, where the salivary glands have completely degraded by this stage.
      (Berry and Baehrecke, 2007)
      hide Interactions
      hide Phenotypic Class
      hideSuppressed by
      Statement
      Reference
      BacA\p35Scer\UAS.cHa, Scer\GAL4en-e16E, WScer\UAS.cYa has mitotic cell cycle defective phenotype, suppressible by NcC318S.Scer\UAS, Scer\GAL4en-e16E
      (Huh et al., 2004)
      BacA\p35Scer\UAS.cHa, Scer\GAL4en-e16E, WScer\UAS.cYa has visible phenotype, suppressible by NcC318S.Scer\UAS, Scer\GAL4en-e16E
      (Huh et al., 2004)
      BacA\p35Scer\UAS.cHa, Scer\GAL4tub has increased cell number | somatic clone phenotype, suppressible by dppd12
      (Perez-Garijo et al., 2005)
      BacA\p35Scer\UAS.cHa, Scer\GAL4tub has increased cell number | somatic clone phenotype, suppressible by wgl-17/dppd12
      (Perez-Garijo et al., 2005)
      BacA\p35Scer\UAS.cHa, Scer\GAL4αTub84B.PZ, rprScer\UAS.cZa has hyperplastic | somatic clone phenotype, suppressible by pucScer\UAS.cMa
      (Ryoo et al., 2004)
      BacA\p35Scer\UAS.cHa, Scer\GAL4en-e16E, WScer\UAS.cZa has mitotic cell cycle defective phenotype, suppressible | partially by panΔN.Scer\UAS
      (Ryoo et al., 2004)
      BacA\p35Scer\UAS.cHa, Scer\GAL4αTub84B.PZ, WScer\UAS.cZa has hyperplastic | somatic clone phenotype, suppressible by pucScer\UAS.cMa
      (Ryoo et al., 2004)
      BacA\p35Scer\UAS.cHa, DpScer\UAS.cDa, E2fScer\UAS.cNa, Scer\GAL4vg.int2.1 has visible phenotype, suppressible | partially by vg[+]/vgnull
      (Legent et al., 2006)
      hideNOT suppressed by
      Statement
      Reference
      BacA\p35Scer\UAS.cHa, Scer\GAL4tub has cell death defective | somatic clone phenotype, non-suppressible by wgl-17
      (Perez-Garijo et al., 2005)
      BacA\p35Scer\UAS.cHa, Scer\GAL4tub has cell death defective | somatic clone phenotype, non-suppressible by dppd12
      (Perez-Garijo et al., 2005)
      BacA\p35Scer\UAS.cHa, Scer\GAL4tub has cell death defective | somatic clone phenotype, non-suppressible by wgl-17/dppd12
      (Perez-Garijo et al., 2005)
      hideEnhancer of
      Statement
      Reference
      BacA\p35Scer\UAS.cHa, Scer\GAL4ey.PH is an enhancer of visible phenotype of DDB1dsRNA.650.Scer\UAS, Scer\GAL4ey.PH
      (Takata et al., 2004)
      BacA\p35Scer\UAS.cHa, Scer\GAL4Act5C.PP is an enhancer of increased cell size | somatic clone phenotype of Scer\GAL4Act5C.PP, bratScer\UAS.cFa
      (Frank et al., 2002)
      BacA\p35Scer\UAS.cHa, Scer\GAL4Act5C.PP is an enhancer of reduced cell number | somatic clone phenotype of Scer\GAL4Act5C.PP, bratScer\UAS.cFa
      (Frank et al., 2002)
      Scer\GAL4en-e16E/BacA\p35Scer\UAS.cHa is an enhancer of increased cell number | embryonic stage phenotype of CycEScer\UAS.cLa
      (Parker, 2006)
      Scer\GAL4en-e16E/BacA\p35Scer\UAS.cHa is an enhancer of reduced cell size | embryonic stage phenotype of CycEScer\UAS.cLa
      (Parker, 2006)
      BacA\p35Scer\UAS.cHa, Scer\GAL4en-e16E is an enhancer of mitotic cell cycle defective | third instar larval stage phenotype of Scer\GAL4en-e16E, pbldsRNA.Scer\UAS
      (Gregory et al., 2007)
      hideNOT Enhancer of
      Statement
      Reference
      BacA\p35Scer\UAS.cHa, Scer\GAL4ptc-559.1 is a non-enhancer of reduced cell number phenotype of Scer\GAL4ptc-559.1, dltdsRNA.Scer\UAS
      (Pielage et al., 2003)
      hideSuppressor of
      Statement
      Reference
      BacA\p35Scer\UAS.cHa, Scer\GAL4Act5C.PP is a suppressor | partially of cell lethal | somatic clone phenotype of DadScer\UAS.cTa, Scer\GAL4Act5C.PP
      (Martin-Castellanos and Edgar, 2002)
      BacA\p35Scer\UAS.cHa, Scer\GAL4hs.2sev is a suppressor of visible phenotype of Scer\GAL4hs.2sev, exScer\UAS.cBa
      (Blaumueller and Mlodzik, 2000)
      BacA\p35Scer\UAS.cHa, Scer\GAL4GMR.PF is a suppressor of cell death increase phenotype of Scer\GAL4GMR.PF, rprGMR.PW, sklScer\UAS.cWa
      (Wing et al., 2002)
      BacA\p35Scer\UAS.cHa, Scer\GAL4GMR.PF is a suppressor of eye color defective phenotype of Scer\GAL4GMR.PF, rprGMR.PW, sklScer\UAS.cWa
      (Wing et al., 2002)
      BacA\p35Scer\UAS.cHa, Scer\GAL4Ubx.PdC is a suppressor of cell lethal phenotype of Scer\GAL4Ubx.PdC, brkScer\UAS.cJa
      (Moreno et al., 2002)
      BacA\p35Scer\UAS.cHa, Scer\GAL4P2.4.Pdf is a suppressor | partially of locomotor rhythm defective phenotype of Scer\GAL4P2.4.Pdf, WScer\UAS.cZa
      (Renn et al., 1999)
      BacA\p35Scer\UAS.cHa, Scer\GAL4GMR.PF is a suppressor | partially of cell death increase phenotype of Scer\GAL4GMR.PF, rprC.Scer\UAS
      (Wing et al., 2001)
      BacA\p35Scer\UAS.cHa, Scer\GAL4GMR.PF is a suppressor | partially of visible phenotype of Scer\GAL4GMR.PF, rprC.Scer\UAS
      (Wing et al., 2001)
      BacA\p35Scer\UAS.cHa, Scer\GAL469B is a suppressor of cell death defective | embryonic stage phenotype of Scer\GAL469B, nmoc5-1.Scer\UAS
      (Mirkovic et al., 2002)
      BacA\p35Scer\UAS.cHa, Scer\GAL4Act5C.PHb is a suppressor of cell death increase phenotype of Scer\GAL4Act5C.PHb, rprScer\UAS.cIa
      (Igaki et al., 2002)
      BacA\p35Scer\UAS.cHa, Scer\GAL4en-e16E is a suppressor of lethal | embryonic stage phenotype of Scer\GAL4en-e16E, rprScer\UAS.cZa
      (Ryoo et al., 2002)
      BacA\p35Scer\UAS.cHa, Scer\GAL4en-e16E is a suppressor of cell death increase phenotype of Scer\GAL4en-e16E, rprScer\UAS.cZa
      (Ryoo et al., 2002)
      BacA\p35Scer\UAS.cHa, Scer\GAL4bi-md653 is a suppressor | partially of visible phenotype of HScer\UAS.cMa, Scer\GAL4bi-md653
      (Mueller et al., 2005)
      BacA\p35Scer\UAS.cHa, Scer\GAL4hs.2sev is a suppressor | partially of cell death increase phenotype of Scer\GAL4hs.2sev, Tak1Scer\UAS.cMa
      (Mihaly et al., 2001)
      BacA\p35Scer\UAS.cHa, Scer\GAL4GMR.PF is a suppressor of lethal phenotype of Scer\GAL4GMR.PF, grim::rprrg.Scer\UAS, sklScer\UAS.cWa
      (Wing et al., 2002)
      BacA\p35Scer\UAS.cHa, Scer\GAL4GMR.PF is a suppressor of eye color defective | conditional - heat sensitive phenotype of Scer\GAL4GMR.PF, grim::rprrg.Scer\UAS, sklScer\UAS.cWa
      (Wing et al., 2002)
      BacA\p35Scer\UAS.cHa, Scer\GAL4GMR.PF is a suppressor of lethal phenotype of Scer\GAL4GMR.PF, grim::rprrg.Scer\UAS
      (Wing et al., 2001)
      BacA\p35Scer\UAS.cHa, Scer\GAL4GMR.PF is a suppressor of visible phenotype of Scer\GAL4GMR.PF, grim::rprrg.Scer\UAS
      (Wing et al., 2001)
      Scer\GAL4Act5C.PHb/BacA\p35Scer\UAS.cHa is a suppressor | partially of cell death increase phenotype of tha.cIa, thcIa
      (Igaki et al., 2002)
      BacA\p35Scer\UAS.cHa, Scer\GAL4en-e16E is a suppressor of cell death increase phenotype of tumΔE1E.Scer\UAS, Scer\GAL4en-e16E
      (Sotillos and Campuzano, 2000)
      BacA\p35Scer\UAS.cHa, Scer\GAL4GMR.PF is a suppressor of visible phenotype of Scer\GAL4GMR.PF, grim::rprgr.Scer\UAS
      (Wing et al., 2001)
      BacA\p35Scer\UAS.cHa, Scer\GAL4GMR.PF is a suppressor of lethal phenotype of Scer\GAL4GMR.PF, grim::rprgr.Scer\UAS
      (Wing et al., 2001)
      BacA\p35Scer\UAS.cHa, Scer\GAL4Ubx.PdC is a suppressor of cell death increase phenotype of Scer\GAL4Ubx.PdC, egrScer\UAS.cMa
      (Moreno et al., 2002)
      BacA\p35Scer\UAS.cHa, Scer\GAL4GMR.PF is a suppressor | partially of cell death increase phenotype of Scer\GAL4GMR.PF, egrScer\UAS.cMa
      (Moreno et al., 2002)
      BacA\p35Scer\UAS.cHa, Scer\GAL4C-765 is a suppressor of cell death increase phenotype of Scer\GAL4C-765, egrScer\UAS.cMa
      (Moreno et al., 2002)
      BacA\p35Scer\UAS.cHa, Scer\GAL4da.G32 is a suppressor of lethal | larval stage phenotype of Scer\GAL4da.G32, imdScer\UAS.cGa
      (Georgel et al., 2001)
      BacA\p35Scer\UAS.cHa, Scer\GAL4hs.PB is a suppressor of cell death increase phenotype of Scer\GAL4hs.PB, imdScer\UAS.cGa
      (Georgel et al., 2001)
      BacA\p35Scer\UAS.cHa, Scer\GAL4en-e16E is a suppressor of visible phenotype of DrefScer\UAS.cSa, Scer\GAL4en-e16E
      (Yoshida et al., 2001)
      BacA\p35Scer\UAS.cHa, Scer\GAL4Bx-MS1096 is a suppressor of visible phenotype of Scer\GAL4Bx-MS1096, ppanScer\UAS.cMa
      (Migeon et al., 1999)
      BacA\p35Scer\UAS.cHa, Scer\GAL4GMR.PF is a suppressor | partially of cell death increase phenotype of Scer\GAL4GMR.PF, grimC.Scer\UAS
      (Wing et al., 2001)
      BacA\p35Scer\UAS.cHa, Scer\GAL4GMR.PF is a suppressor | partially of visible phenotype of Scer\GAL4GMR.PF, grimC.Scer\UAS
      (Wing et al., 2001)
      BacA\p35Scer\UAS.cHa, Scer\GAL4P2.4.Pdf is a suppressor | partially of locomotor rhythm defective phenotype of Scer\GAL4P2.4.Pdf, rprScer\UAS.cZa
      (Renn et al., 1999)
      BacA\p35Scer\UAS.cHa, Scer\GAL4en-e16E is a suppressor of cell death increase phenotype of Scer\GAL4en-e16E, modScer\UAS.cPa
      (Perrin et al., 2003)
      BacA\p35Scer\UAS.cHa, Scer\GAL4GMR.PF is a suppressor of visible phenotype of Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF
      (Warrick et al., 1998)
      Scer\GAL4bbg-C96/BacA\p35Scer\UAS.cHa is a suppressor | partially of visible phenotype of ct6
      (Krupp et al., 2005)
      Scer\GAL4αTub84B.PBb/BacA\p35Scer\UAS.cHa is a suppressor of cell death increase | somatic clone | cell non-autonomous phenotype of dmScer\FRT.αTub84B.PBb
      (Moreno and Basler, 2004)
      Scer\GAL4ninaE.PHS, Scer\GAL4ninaE.PHS, BacA\p35Scer\UAS.cHa is a suppressor | partially of cell death increase phenotype of Arr11