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General Information
Symbol
BacA\p35UAS.cHa
Species
N. Autographa californica nucleopolyhedrovirus
Name
Saccharomyces cerevisiae UAS construct a of Hay
FlyBase ID
FBal0062158
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-P35, UASP35, UAS::p35, p35, UAS p35, P[UAS-p35], UAS-p35.H, UAS:p35, P{w+mC=UAS-p35.H}, UAS-P53
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS regulatory sequences drive expression of BacA\p35.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
glial cell | ectopic & antennal disc, with Scer\GAL4Dll-981
glial cell | ectopic & antennal disc, with Scer\GAL4lz-gal4
glial cell | ectopic & antennal disc, with Scer\GAL4pros.PMG
glial cell | ectopic | conditional ts & antennal disc, with Scer\GAL4hs.PB
macrochaeta | ectopic & scutellum, with Scer\GAL4pnr-MD237
macrochaeta | supernumerary & larva, with Scer\GAL4hs.PB
neuron & ventral nerve cord, with Scer\GAL4Crz.PC
Detailed Description
Statement
Reference
Expressing BacA\p35UAS.cHa under the control of Scer\GAL4GMR.PU does not have an obvious effect on eye size.
The expression of BacA\p35UAS.cHa from the second instar larval stage under the combined control of Scer\GAL4ap-md544 and Gal80[ts] does not significantly affect the size of the third instar larval wing disc dorsal compartment, nor their average centrosome number per cell, as compared to controls.
Adults expressing BacA\p35UAS.cHa under the control of Scer\GAL4elav.PLu do not exhibit climbing defects compared to controls.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en-e16E does not affect the size of the adult wing posterior compartment, as compared to controls.
The expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4pnt-14-94 does not lead a significant change in the number of type II neuroblasts per third instar larval brain lobe, as compared to controls.
The expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4ey.PU does not affect the mitotic index in the third instar larval eye disc, as compared to controls.
Third instar larval brain expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4insc-Mz1407 do not exhibit significant volume changes, as compared to controls.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4rn.PU results in a small but significant reduction in area of the adult wing. Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en.PU results in a small but significant reduction in area of the posterior compartment of the adult wing.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4elav-C155, but not Scer\GAL4repo-M1B, results in a decrease of the number of apoptotic cells in the optic lobe from 0h APF to 48h APF as compared to wild type.
Upon exposure to ionizing radiation, individuals expressing BacA\p35UAS.cHa under the control of Scer\GAL4en.PU display wing disc posterior compartments that are significantly enlarged, present basal cell delamination and present BacA\p35UAS.cHa-expressing cell far away from the domain of expression; these phenotypes are not observed under non-irradiated conditions.
Expression of BacA\p35Scer\UAS.cHa driven by Scer\GAL4bi.PU does not significantly affect wing discs.
Expression via Scer\GAL4wor.PA and Scer\GAL80ase.PN does not prevent the normal disappearance of pupal neuroblasts.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Sgs3.PD does not block the formation of the large acidic structures which normally occurs in salivary gland cells approximately 1.5 hours after head eversion, but caspase activation at 2 hours after head eversion is blocked. This does not prevent breakdown of the salivary gland tissue, as no persistent salivary gland phenotype is seen at 24 hours after puparium formation.
Expression of five copies of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4VGlut-OK371 does not alter the rate of axon degeneration in the L1 wing vein neurons following axotomy compared to wild type.
Expression of BacA\p35Scer\UAS.cHa in the developing eye, under the control of Scer\GAL4GMR.PS does not affect compound eye morphology.
Expression of BacA\p35Scer\UAS.cHa under the simultaneous control of both Scer\GAL4VGlut-OK371 and Scer\GAL4tub.PU results in an increase in the number of neurons derived from LinA neuroblast clones.
Egg chambers expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4os.cXa have an increased number of polar cells per cluster compared to wild type; all wild type egg chambers have two polar cells per cluster, whereas 35% of polar cell clusters contain more than two cells.
11.1 +/- 1.3 surviving EW3-sib cells are seen in larvae expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en.PU (these cells die during embryogenesis in wild type).
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4CCAP.PP prevents the programmed cell death of CCAP neurons in the adult ventral nerve cord after eclosion.
Expression of BacA\p35UAS.cHa under the control of Scer\GAL4NP5130 has no obvious effect on the proportion of Esg-positive progenitors in the adult posterior midgut.
Caspase activity is significantly inhibited by BacA\p35Scer\UAS.cHa overexpression (under the control of Scer\GAL4Lsp2.PH).
Expression of BacA\p35Scer\UAS.cHa in larval wing disc cells, under the control of Scer\GAL4ptc-559.1, do not display invasive cell behaviour, but contain occasional cells with processes extended towards the posterior compartment.
Blocking caspase activity in neurons through expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4elav.PU results in embryos with a reduced volume of apoptotic particles and reduced engulfment of apoptotic neurons compared to controls.
Male flies expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 exhibit slightly increased levels of spermatogonial cyst death compared to controls.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en-e16E has no deleterious effect on wing disc development.
Expression of BacA\p35Scer\UAS.cHa in the mushroom bodies under the control of Scer\GAL4c739 does not have any effect on response to heat shock stress.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4GMR.PF has no effect on eye pigmentation.
Males expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en.PU display mis-oriented adult genitalia. During pupal development, the genital disc does not undergo full rotation. Rotation commences as normal and the initial average velocity is comparable to control flies. However rotation stops before it is complete and the acceleration-stage of rotation is lower compared to controls. The inner ring of the disc rotates normally in these animals, but the rotation of the outer ring is impaired.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Crz.504 blocks developmental programmed cell death in vCrz neurons.
Adult brains of animals containing clones expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub.PU do not show cell accumulation in the medulla cortex or alteration in the medulla neuropil lamination.
Overexpression of BacA\p35Scer\UAS.cHa in neuroblasts under the control of Scer\GAL4insc-Mz1407 does not affect neuroblast number.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4elav.PU does not result in a significant change in fly survival or climbing ability, as compared to wild type.
Class III neurons overexpressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL419-12 do not undergo apoptosis and are still present by 18 hours after puparium formation.
Blocking apoptosis through expression of BacA\p35Scer\UAS.cHa in the wing margin cells under the control of Scer\GAL4wg-IS650 (with Scer\GAL80ts.αTub84B blocking expression at other times), results in survival of the flat cells and no ziz-zag alignment of the wing margin cells, as found in wild-type. Expression of BacA\p35Scer\UAS.cHa in the pupal wing, under the control of Scer\GAL4wg-IS650 (with Scer\GAL80ts.αTub84B blocking expression at other times), results in misalignment of hair shafts, with some aligned in an almost side-by-side manner by chance. Consistent with this, in the adult wing expressing BacA\p35Scer\UAS.cHa, the alternate projection of the hairs is disturbed, and hairs from the dorsal and ventral compartments overlap. Blocking apoptosis also affects the position of the sensory bristles in the anterior wing margin, leading to overlap of the double-row hairs, although the phenotypic relationship between the anterior and posterior wing margins is unclear. Expression of BacA\p35Scer\UAS.cHa in the pupal wing, under the control of Scer\GAL4sd-SG29.1 results in the loss of TUNEL-positive nuclei at 24h after pupal formation.
Pruning of ddaC dendrites is delayed upon expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4ppk.1.9. The degeneration of dendrites after severing is unaffected however.
The A7 spiracle, which in wild-type males is associated with the A6 tergite, remains posterior to A6 in adult males expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4dsx-GAL4. No A7 tergite develops in these mutants. However, there is excess pleural tissue posterior to the A6 tergite. Furthermore, the A6 tergite of the mutants is significantly narrower than A6 of control males.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4GMR.PU does not affect photoreceptor and cone cell differentiation.
Overexpression of BacA\p35Scer\UAS.cHa in presynaptic neurons under the control of Scer\GAL4OK6 has no obvious effect on synapse elimination. Overexpression of BacA\p35Scer\UAS.cHa in postsynaptic neurons under the control of Scer\GAL4C57 results in strong arrest of synapse elimination at 7 hours after puparium formation.
The number of SELK neurons is normal in animals expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4elav-C155.
Scer\GAL4Switch1.PC-driven expression of BacA\p35Scer\UAS.cHa delays the developmental disappearance of midgut peripheral cells and differentiation of adult midgut progenitors.
Animals expressing BacA\p35Scer\UAS.cHa in the salivary glands under the control of Scer\GAL4fkh.PH display persistent condensed salivary gland cell fragments in 94% of pupae, and persistent salivary gland fragments in 6% of pupae.
dsx-SN and dsx-TN2 cells are protected from programmed cell death in adult females expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4dsx.KI as the number of these neurons is not significantly different from that of wild-type males in these females (these neurons are normally present in both sexes in 48 hour pupae but are specific to males in the adult). Additional neurons are observed in dsx-pC2 and dsx-pC3 clusters in females expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4dsx.KI and cells of the TN1 cluster are also present (these cells are never observed in wild-type females). TN1 and TN2 neurons present in these females have contralateral projections that are ordinarily seen only in wild-type males.
BacA\p35Scer\UAS.cHa expression driven by Scer\GAL4Abd-B-LDN has no effect on the dorsal fusion of the abdominal A8 segment during male genitalia morphogenesis.
Expression of BacA\p35Scer\UAS.cHa in the C4da neurons (using the Scer\GAL4ppk.PG line) leads to a reduction in dendritic branch pruning at 18 hours after puparium formation.
Mushroom body neuroblasts are seen in the brains of young adults expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4wor.PA, although their persistence is only transient (mushroom body neuroblasts are not seen in wild-type adults).
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Abd-B-LDN results in a high proportion of males having a half-rotated genitalia (180[o] rotation) phenotype. Time-lapse imaging reveals that the A8a ring (which normally rotates) in the genital disc stays mostly still during the whole process, whereas the A8p ring in the genital disc rotates normally. Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4hh-Gal4 results in a high proportion of males having a half-rotated genitalia (180[o] rotation) phenotype. Expression of BacA\p35Scer\UAS.cHa simultaneously under the control of both Scer\GAL4Abd-B-LDN and Scer\GAL4hh-Gal4 results in 40% of males showing non-rotated genitalia, while the remainder show 90[o] rotation.
In contrast to controls, the amnioserosa of Scer\GAL4da.G32 BacA\p35Scer\UAS.cHa embryos persists as an intact coherent tissue beyond the 4-lobe midgut stage and beyond the onset of somatic musculature innervation. This phenotype is frequently observed in mutant embryos that are greater than 24 hours old and still alive, indicating that the amnioserosa can persist at least 8 hours beyond its normal time of degeneration. Scer\GAL4da.G32 BacA\p35Scer\UAS.cHa embryos show no TUNEL-positive cells in the amnioserosa or other tissues. Scer\GAL4Orct2-cald-GAL4 BacA\p35Scer\UAS.cHa embryos exhibit a strong, persistent amnioserosa phenotype. Autophagy still occurs in embryos expressing BacA\p35Scer\UAS.cHa using Scer\GAL4da.G32 or Scer\GAL4Orct2-cald-GAL4.
Expression of BacA\p35Scer\UAS.cHa in adults under the control of Scer\GAL4Act5C.Switch.PR does not significantly decrease life span. Expression of BacA\p35Scer\UAS.cHa during larval development under the control of Scer\GAL4Act5C.Switch.PR significantly reduces the mean life span of adults. A subset of female flies show unchanged or increased lifespan. Expression of BacA\p35Scer\UAS.cHa in the adult nervous system under the control of Scer\GAL4elav.Switch.PO does not effect lifespan. Expression of BacA\p35Scer\UAS.cHa in larvae under the control of Scer\GAL4elav.Switch.PO is associated with significant decreases in life span in both male and female flies. A significant reduction in the number of male flies is observed.
Flies expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4GMR.PF show an increase in the size of the eye and also in the size of the ommatidia within the eye compared to control flies.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4pnr-MD237 has no affect on the adult notum.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4109-53 results in an abnormal stalk between egg chambers. The stalk contains more cells than normal and the cells are not properly arranged into a one-cell wide stalk.
At 24 hours after puparium formation, animals expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4fkh.PH still contain salivary gland tissue, in contrast to wild-type animals, where the salivary glands have completely degraded by this stage.
Hemolymph clots from third instar larvae expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4He.PZ show much reduced melanization levels.
Scer\GAL430A-driven expression of BacA\p35Scer\UAS.cHa prevents migration of most cells and blocks bonding of the wing surfaces but does not appear to affect delamination from the cuticle. The cells remain within the wing suspended in haemolymph in a round state. In flies heterozygous for BacA\p35Scer\UAS.cHa, many fewer cells remain within the wing. These cells are round and tightly bound within the wing due to bonding of the wing surfaces; they eventually found brown spots in the wing as the fly ages.
Stage 16 embryos expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tey-5053A have normal somatic muscle morphology.
Blocking apoptosis in larval epidermal cells by clonal expression of BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4Act5C.PI, results in impaired extrusion of these cells from the epidermis, and failure of haemocyte recruitment and the engulfment process. The few larval epidermal cells that are able to undergo extrusion remain as viable cells under the epithelial layer. Concomitantly, there is a significant decrease in the progression of histoblast nest spreading and in the average number of histoblasts. Some of these animals survive to pharate adults, but survivors show abdominal cuticle clefts with many larval epidermal cells still present.
The number of Rh5 photoreceptors in the Bolwig's organ at the end of embryogenesis is unchanged relative to wild type in animals expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4so-7.GAL4. However, the number of Rh6 photoreceptors is dramatically increased to 20-25 instead of the normal number of 8-10.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Tab2-201Y has no effect on the pruning of the axon branches or on the engulfing action of glia in the dorsal lobes of the mushroom body during metamorphosis.
Flies expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Mhc.PW show normal wing positions.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Crz rescues apoptosis of Crz-expressing neurons in the ventral nerve cord during pupation.
The overall morphology of wing discs expressing BacA\p35Scer\UAS.cHa in the posterior compartment under the control of Scer\GAL4hh-Gal4 is normal except for occasional local tissue overgrowth.
Expression of BacA\p35Scer\UAS.cHa in the salivary glands under the control of Scer\GAL434B delays salivary gland histolysis during pupal development.
Expression of BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4en-e16E leads to vein loss in the adult wing.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PI does not affect S2 cell morphology.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4fkh.PH does not affect salivary gland morphogenesis in embryos.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4ey.PH in wild-type eyes does not affect eye size.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4dpp.blk1 in the wing disc has no effect on cell morphology.
Overexpression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4ppk.1.9 suppresses dendritic branch removal.
Expression of BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4elav-C155, does not increase lifespan in flies.
Inhibition of caspases in the scutellum, achieved by expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4sca-P309, results in the formation of ectopic macrochaetae on this body part in 39% of cases. Ectopic macrochaetae also appear in 23% of cases when BacA\p35Scer\UAS.cHa is expressed under the control of Scer\GAL4dpp.blk1. In contrast, the ectopic expression of BacA\p35Scer\UAS.cHa in SOP cells, driven by Scer\GAL4neur-GAL4-A101, does not result in extra macrochaetae. No significant increase in macrochaetae number is seen when BacA\p35Scer\UAS.cHa is expressed under the control of Scer\GAL4hs.PB following a heat shock at late third-instar (6-12 h APF), which is during SOP cell formation, or after it (24-30 h APF). At the stage prior to the appearance SOP cells, however, BacA\p35Scer\UAS.cHa expression leads to extra macrochaetae formation.
Expression of BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4en-e16E, results in misorientation of male external genitalia, while Scer\GAL4ptc-559.1-driven expression leads to an increased number of bristles. Over 96% of wings in Scer\GAL4en-e16E>BacA\p35Scer\UAS.cHa flies appear normal.
Expression of BacA\p35Scer\UAS.cHa in the posterior of the wing disc, driven by Scer\GAL4en-e16E, has little effect on the patterning of adult wings.
X-ray irradiated wing disc clones expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub contain a mixture of two types of cell: `live' cells, which have not initiated apoptosis due to the irradiation, and `undead' cells (between 20 and 70% of the clone), which have initiated apoptosis, but have failed to complete programmed cell death. No such cells persist outside the clones in which BacA\p35Scer\UAS.cHa is expressed. The `live' cells in the X-ray irradiated clones display increased proliferation, while the `undead' cells exhibit an abnormally slow rate of proliferation. X-ray irradiated wing disc clones expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub are also associated with local enhancements of growth, with approximately 43% of wing discs exhibiting local deformations and small outpouchings of extra tissues associated with the clones.
Expression of BacA\p35Scer\UAS.cHa in the primordial germ cells (PGCs) in the embryo under the control of Scer\GAL4nos.PG does not affect PGC death.
Cell proliferation is not increased in the posterior compartment of wing discs expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en-e16E. Wings appear normal in adults expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en-e16E.
In the wings of BacA\p35Scer\UAS.cHa; Scer\GAL4en-e16E flies many cells persist between the dorsal and ventral cuticular sheets.
Apoptosis is eliminated and extra photoreceptor clusters are formed at the edge of the developing eye in BacA\p35Scer\UAS.cHa; Scer\GAL4GMR.PF animals 42 hours after puparium formation. These clusters survive to form ommatidia in adult flies, but are often incomplete.
Males show rotated terminalia and a gap in the dorsal midline.
BacA\p35Scer\UAS.cHa; Scer\GAL4nub-AC-62 suppresses basal levels of apoptosis in the wing pouch.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Vap.P0201 efficiently suppresses the cell death that is normally seen in anterior dMP2 neurons in late stage embryos, such that these neurons are present in late stage embryos.
Flies expressing BacA\p35Scer\UAS.cHa under the control of one of Scer\GAL4nub-AC-62, Scer\GAL4ap-md544, Scer\GAL4hh-Gal4 or Scer\GAL4en-e16E have virtually normal wings. Wing vein L5 is slightly shortened in animals expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4hh-Gal4. Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4ap-md544 prevents wing death in the dorsal, but not the ventral, compartment of the wing disc. Animals expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4hh-Gal4 which have been irradiated during the first larval instar show defects in the wing disc; the posterior compartment is abnormal in shape and increased in size. The few adults that do hatch differentiate wings with very abnormal and overgrown posterior compartments. Wing discs of animals expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4hh-Gal4 which have been irradiated during the first or second larval instar show an increase in the ratio of the size of the posterior compartment/anterior compartment compared to control discs and show greater BrdU incorporation in the posterior compartment after irradiation. In addition, cells in the anterior compartment close to the anterior-posterior (AP) border also show an increase in BrdU incorporation. The AP border becomes highly irregular in these irradiated discs and cells of posterior provenance often penetrate into the anterior compartment. These invading posterior cells do not readily mix with anterior ones, but tend to stay in separate groups. Clones in the wing disc expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PI which have been induced during the first or second larval instar and then irradiated 24 hours later, show heterogeneous incorporation of BrdU inside the clone. There is often an increase of BrdU levels in the vicinity of these clones.
When BacA\p35Scer\UAS.cHa is driven by Scer\GAL4Dll-981 in the antennal disc >300 glial cells are formed as opposed to the ~100 in wild-type. The organisation of sensory afferent fascicles from the antenna is unaffected. When BacA\p35Scer\UAS.cHa is driven by Scer\GAL4lz-gal4 in the antennal disc a significant increase in glial cell number is seen. When BacA\p35Scer\UAS.cHa is driven by Scer\GAL4pros.PMG in the antennal disc a significant increase in glial cell number is seen. When BacA\p35Scer\UAS.cHa is driven by Scer\GAL4ato.3.6 in the antennal disc no alteration is seen in the number of glial cells. When BacA\p35Scer\UAS.cHa is driven by Scer\GAL4hs.PB and heat pulsed up to 6-8 hours after puparium formation (AFP) no significant alterations in glial number are seen. While pulsing during the 14 to 18 AFP period results in a very large increase in glial cell numbers.
Expression of BacA\p35Scer\UAS.cHa, driven by Scer\GAL4Ddc.PL, results in a statistically significant increase in the number of both serotonergic and dopaminergic neurons after metamorphosis.
When BacA\p35Scer\UAS.cHa is driven in the wing disc by Scer\GAL4C10, no effect on the average size of the wing disc is seen. However significantly variability is in the size of the wing disc.
BacA\p35Scer\UAS.cHa; Scer\GAL4Hsp83.PA males are infertile (males carrying either construct alone are not). Although the initial assembly of the "individualisation complex" at the head of the cyst appears normal, there is a failure of cytoplasm to collect into a normal cystic bulge.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Cg.PA does not cause an appreciable increase in hemocyte number in third instar larvae.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4neur-P72 produces supernumerary polar cells in advanced egg chambers. Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PP in clones in the egg chamber results in the production of extra polar cells. No obvious defects are seen in the early development of these egg chambers. 80% of stage 9 egg chambers expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PP have round anterior follicle cells, a constant thickness of epithelial cells along the anterior-posterior axis and a homogeneous distribution of anterior follicle cell nuclei (in contrast to wild-type stage 9 egg chambers which have elongated anterior follicle cells, thinning of the epithelium at the anterior end and a reduction in density of follicle cell nuclei at the anterior pole). 91% of stage 10 egg chambers expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PP have defects in delamination and migration of border cells; the border cells are either still stuck at the anterior pole or are only partway between the anterior pole and the oocyte. These clusters often contain supernumerary border cells.
When BacA\p35Scer\UAS.cHa is driven by Scer\GAL4αTub84B.PL in scrib1 clones in the eye, the amount of mutant tissue surviving to adulthood increases compared with scrib1 clones alone.
In stage 16 female gonads, where expression of BacA\p35Scer\UAS.cHa is driven by both Scer\GAL4how-24B and Scer\GAL4twi.PB, these gonads appear masculinized; male-specific somatic gonadal precursors persist and join the posterior of female gonads, as in wild-type male embryos. The presence of such cells does not appear to affect ovary formation or oogenesis, since embryos develop into fertile adult females.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4neur-P72 suppresses the fragmentation of the glial cell which is normally seen in the thoracic microchaete lineage in the developing notum; at 24 hours after puparium formation (APF), 86% of the clusters contain glial cells in these animals (compared to 8% of clusters in control animals). The ectopically surviving glial cells are tightly associated with axonal processes in 72% of sensory organs studied at 24 hours APF (in 55% of cases the glial cells are located at the growth cone, while in the remaining 17.5% they are associated with the axon), while in 27.5% of the sensory organs studied the glial cells remain within the proximity of the cluster. At 27 hours APF, the axonal network of the notum and its orientation shows no major difference between wild-type pupae and pupae expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4neur-P72. Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4neur-P72 promotes precocious axonogenesis; axonal growth cones of notal microchaetae are observed 23.5 hours APF in these flies, compared to 25 hours APF in wild type. The cleaning reflex is normal in adults expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4neur-P72.
When BacA\p35Scer\UAS.cHa is expressed under the control of Scer\GAL4sim.PS an increase in the number of midline glial cells is seen. About 6 cells are seen per neuromere.
When driven by Scer\GAL4OK107 or Scer\GAL4Tab2-201Y no defects in axon pruning or the mushroom body are seen.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4GMR.PF results in extra interommatidial cells in the eye disc.
Embryos expressing BacA\p35 using Scer\GAL469B with BacA\p35Scer\UAS.cHa have severe head defects, while denticle patterning appears fairly normal. Cell death is completely blocked in these embryos.
BacA\p35Scer\UAS.cHa; Scer\GAL4C-765 flies have no discernible wing phenotype. BacA\p35Scer\UAS.cHa; Scer\GAL4GMR.PF flies have subtle ommatidial disorganisation.
In BacA\p35Scer\UAS.cHa; Scer\GAL4arm.PS embryos, an ectopic external sensory organ forms near the abdominal ventral multidendritic neuron a1 neuron in many abdominal segments.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4pnr-MD237 results in extra macrochaetae in the scutellum in 30% of animals.
When expression is driven by Scer\GAL4fkh.PH salivary glands survive the cell death that would have been their normal fate. DNA fragmentation is suppressed, though they have progressed to a late stage of autophagy (i.e. vacuoles and associated plasma membranes are often absent from the cytosol).
Expression of BacA\p35Scer\UAS.cHa in the scutellum under the control of Scer\GAL4ptc-559.1, Scer\GAL4dpp.blk1 or Scer\GAL4sca-537.4 results in the formation of ectopic bristles.
When expression is driven by Scer\GAL4cCa.T:Hsim\VP16 in mitotic domain 20 at gastrulation an expansion of the dorsal surface epithelium occurs, though cell death still occurs. Defects are mild compared to those of Df(3L)H99 embryos.
Scer\GAL4D59-mediated expression causes failure of salivary gland histolysis in 15 hour prepupae, the salivary glands can still be detected 22 hours after puparium formation. The salivary glands at this stage are abnormal in morphology and appear to be partially degraded.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
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NOT Suppressor of
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Phenotype Manifest In
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BacA\p35UAS.cHa, Scer\GAL4Dll-981 has glial cell | ectopic & antennal disc phenotype, suppressible by lz3
BacA\p35UAS.cHa, Scer\GAL4Dll-981 has glial cell | ectopic & antennal disc phenotype, suppressible | partially by ato1/Df(3R)p13
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Scer\GAL4elav-C155/BacA\p35UAS.cHa is a suppressor | partially of larval thorax & neuroblast | somatic clone phenotype of grhB37
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Scer\GAL4Tub.PU/BacA\p35UAS.cHa is a non-suppressor of cell & larval salivary gland | somatic clone phenotype of miΔEY22
Scer\GAL4Tub.PU/BacA\p35UAS.cHa is a non-suppressor of nucleus & cell & larval salivary gland | somatic clone phenotype of miΔEY22
BacA\p35UAS.cHa, Scer\GAL4en-e16E is a non-suppressor of wing | posterior | heat sensitive phenotype of Scer\GAL4en-e16E, brkUAS.cMa
Scer\GAL4ey.PH/BacA\p35UAS.cHa is a non-suppressor of eye phenotype of eyg1/eygM3-12
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Additional Comments
Genetic Interactions
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The co-expression of BacA\p35Scer\UAS.cHa partially suppresses the severe decrease in size induced in the third instar wing disc posterior compartment by the expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4en-e16E.
Overexpression of BacA\p35Scer\UAS.cHa rescues the fusion of retinal cone cells induced by cazKK107486 expression. Co-expression of BacA\p35Scer\UAS.cHa results in partial suppression of the severe rough eye phenotype seen in flies expressing cazKK107486 under the control of Scer\GAL4GMR.PS. Apoptotic cells detected in caz knockdown retinae are significantly reduced in flies expressing BacA\p35Scer\UAS.cHa.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en.PU or Scer\GAL4hh.PU, in a mad2GD16427 mutant background, results in little cell death. There is a significant reduction in chromosome instability tolerance induced by mad2GD16427 expression, when mbtGD9608 is expressed in imaginal wing discs under the control of Scer\GAL4en.PU or Scer\GAL4hh.PU. There is also a concomitant increase in cell death in these double mutants. This cell death is inhibited by the addition of BacA\p35Scer\UAS.cHa. There is a significant reduction in chromosome instability tolerance induced by mad2GD16427 expression, when Tak1KK108611 is expressed in imaginal wing discs under the control of Scer\GAL4en.PU or Scer\GAL4hh.PU. This is concomitant with an increase in the level of cell death. Consistent with high levels of cell death and consequent loss of tissue, adult wings show notching when JNK signalling is reduced in a chromosome instability background (through expression of mad2GD16427). This cell death is inhibited by the addition of BacA\p35Scer\UAS.cHa. Expression of bskKK108156 in the posterior wing disc compartment of flies expressing mad2GD16427 (with both lines under the control of either Scer\GAL4en.PU or Scer\GAL4hh.PU) results in both chromosome instability and high levels of cell death. This cell death is inhibited by the addition of BacA\p35Scer\UAS.cHa.
Overexpression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not affect cell death caused by expression of lokScer\UAS.P\T.T:Ivir\HA1. Overexpression of BacA\p35Scer\UAS.cHa with p53Scer\UAS.P\T.cBa, during oogenesis under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not suppress the p53Scer\UAS.P\T.cBa-dependent stem cell loss.
Expression of BacA\p35Scer\UAS.cHa in the developing retina under the control of Scer\GAL4ey.PU suppresses the retinal degeneration phenotype (and associated loss of eyesight) found in NnaDPL90 mutants, indicating that altered apoptotic pathway activity is contributing to the NnaDPL90 escaper male phenotype.
Expression of BacA\p35Scer\UAS.cHa suppresses the rough eye phenotype seen when Dcp-1Scer\UAS.cKa (insertion line P{UAS-Dcp-1.K}19-2) is expressed under the control of Scer\GAL4GMR.PF.
Ectopic caspase activation in the posterior compartment of the developing wing discs resulting from expression of hppyScer\UAS.cLa under the control of Scer\GAL4e22c is completely suppressed through co-expression of BacA\p35Scer\UAS.cHa.
Suppression of apoptosis in grhB37 clones, achieved by expression of BacA\p35Scer\UAS.cHa driven by Scer\GAL4elav-C155, results in the continued presence of the neuroblast at 96 hours in over 40% of cases. However, this rescued neuroblast is often larger in size than wild-type clones.
Expression of BacA\p35Scer\UAS.cHa in the posterior compartment of pixL35/pixL17 wing discs, driven by Scer\GAL4en-e16E, increases the area of the posterior compartment relative to control wings that also express BacA\p35Scer\UAS.cHa in the posterior. Expression of BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4en-e16E, in pixL17 wing disc clones increases the frequency of the clones but does not increase their size.
BacA\p35Scer\UAS.cHa does not suppress the reduction of wing pouch size seen in brkScer\UAS.cJa; Scer\GAL4nub-AC-62 wing discs - even when 2 copies of BacA\p35Scer\UAS.cHa are present.
Xenogenetic Interactions
Statement
Reference
Individuals co-expressing BacA\p35UAS.cHa and HipkUAS.Tag:HA under the control of Scer\GAL4dpp.blk1 arrest at the third larval stage for an extended period of time (beyond 10 days) and eventually die as larvae. BacA\p35UAS.cHa co-expression enhances the wing disc tumorigenesis of the expression of HipkUAS.Tag:HA expression (the Dpp expression domain cells expand substantially and occupy almost the entire disc) and does not suppress the melanotic masses.
The smaller third instar larval wing disc dorsal compartment and associated polyploidy/aneuploidy induced by the expression of pnutGD1512 from the second instar larval stage under the combined control of Scer\GAL4ap-md544 and Gal80[ts] are respectively suppressed and enhanced by the co-expression of BacA\p35UAS.cHa; this pnutGD1512, BacA\p35UAS.cHa co-expression leads to a severe decrease in the number of cells undergoing DNA replication (assessed by EdU labelling and phospho-H3 immunostaining), as compared to controls. The expression of BacA\p35UAS.cHa in combination with chbHMS01146, diaHMS00308, pblJF02979 or IncenpGD7511 from the second instar larval stage under the combined control of Scer\GAL4ap-md544 and Gal80[ts] leads to an increase in the average centrosome number per cell in the third instar larval wing disc dorsal compartment, as compared to controls.
The decreased numbers of cardioblasts (namely generic cardioblasts) in SB0453 mutant embryos are partially suppressed by the additional expression of BacA\p35UAS.cHa under the control of Scer\GAL4how-24B.
Co-expression of BacA\p35Scer\UAS.cHa leads to enhancement of the wing posterior compartment size reduction phenotype seen in adult wings of flies expressing p115KK100066 under the control of Scer\GAL4en-e16E. Co-expression of BacA\p35Scer\UAS.cHa suppresses the increase cell death, but does not suppress the increased numbers of cells in S phase and mitosis seen in third instar wing discs expressing p115KK100066 under the control of Scer\GAL4hh.PU, and additionally leads to an average increase in cell size and an overall increase in the DNA content in the cells in the posterior wing disc, as compared to controls.
The co-expression of BacA\p35Scer\UAS.cHa does not suppress the decreased number of type II neuroblasts in third instar larval brains expressing Su(Tpl)HMS00277 under the control of Scer\GAL4insc-Mz1407.
The eye degeneration and the associated rough eye appearance, ommatidial fusion and pigmentation loss characteristic for adult flies expressing Hsap\FUSScer\UAS.cIa under the control of Scer\GAL4GMR.PS cannot be rescued by co-expression of BacA\p35Scer\UAS.cHa.
The co-expression of BacA\p35Scer\UAS.cHa enhances the retinal differentiation inhibition, but not the increased mitotic index, induced by the expression of Cdc16GD12028 under the control of Scer\GAL4ey.PU.
The co-expression of BacA\p35Scer\UAS.cHa rescues the decreased lifespan, the decreased food intake, the decreased gut contractions, the dilated intestine, and the visceral muscle structure defects (including in actin filaments) observed in adults expressing mstJF03280 under the control of Scer\GAL4Mef2.PU.
The loss of multidendritic ddaC neurons in third instar larvae induced by Ect4ΔARM.Scer\UAS.T:Hsap\MYC expression (with the Scer\GAL4ppk.PG driver) in third instar larvae as well as neurite degeneration in Pdf-positive neurons in adult flies (under the Scer\GAL4P2.4.Pdf driver combined with tub-Gal80[ts] to restrict the expression to adulthood) cannot be rescued by co-expression of BacA\p35Scer\UAS.cHa.
The expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4insc-Mz1407 partially suppresses the decreased brain volume of mad2G6595, Sas-4s2214 double homozygous third instar larvae.
The shortening of the dendritic arbor in class IV ddaC neurons in third instar larvae expressing prelScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4109(2)80 is only slightly mitigated by co-expression of BacA\p35Scer\UAS.cHa.
Co-expression of aptScer\UAS.cEa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4ap-md544 leads to overgrowth of the wing disc, as compared to controls.
The reduced number of ensheathing glia in the developing antennal lobes at late pupal stages induced by Scer\GAL4SPARC-MI00329-GAL4-controlled expression of htlGD14457 is suppressed by co-expression of BacA\p35Scer\UAS.cHa, this however does not rescue either the altered wrapping pattern of the ensheathing glial cells or the disrupted glomerular compartmentalization.
Co-expression of any of the following: rprScer\UAS.C, brmHMS00050 and osaHMS01738 together with BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act.PU in somatic (flip-out) clones in third instar larval wing discs does not cause tumorigenic overgrowth. Co-expression of Snr1GD4140 and BacA\p35Scer\UAS.cHa driven by Scer\GAL4Act.PU in somatic (flip-out) clones or controlled by Scer\GAL4ptc.PU in the wing disc of third instar larvae leads to increased cell proliferation and massive tissue overgrowth. Co-expression of Snr1HMS00363 and BacA\p35Scer\UAS.cHa driven by Scer\GAL4Act.PU in somatic (flip-out) clones in either wing or eye discs of third instar larvae leads to aggressive tissue overgrowth. This overgrowth is (in the wing discs) significantly suppressed by co-expression of any of the following: Stat92EdsRNA.Scer\UAS, NJF02959, pucScer\UAS.cMa or bskDN.Scer\UAS.cUa but not by co-expression of either Ilp8GD1670 or Mmp1JF01336.
Co-expression of BacA\p35Scer\UAS.cHa restores the number of mushroom body neuroblasts, and partially restores the number of Kenyon cells to normal in flies expressing RxGD4076 under the control of Scer\GAL4insc-Mz1407 at 72 h APF.
The increased frequency of aneuploid metaphases observed in third instar larval wing discs expressing vtdGL00522 under the control of Scer\GAL4en-e16E is increased further by co-expression of BacA\p35Scer\UAS.cHa.
The additional co-expression of BacA\p35Scer\UAS.cHa rescues the increased apoptosis in the dorsal compartment of third instar larval wing discs co-expressing HPV18\E6Scer\UAS.T:Hsap\MYC and Hsap\UBE3AScer\UAS.cRa under the control of Scer\GAL4ap.PU and induces basal delamination of high-expressing cell clusters, but does not lead to disc overgrowth, as compared to controls.
The enlarged wing disc posterior compartment observed upon exposure to ionizing radiation of individuals expressing BacA\p35UAS.cHa under the control of Scer\GAL4en.PU is enhanced by the co-expression of either okrKK107487, grpGD4189, grpKK108548, mei-41GD4258 or mei-41KK101792; the phenotype observed upon the expression BacA\p35UAS.cHa alone or in combination with either okrKK107487 or mei-41GD4258 is also suppressed by the additional co-expression of bskK53R.UAS; the phenotype observed upon the expression BacA\p35UAS.cHa alone is not significantly affected by DNAlig4169 homozygosity. The Scer\GAL4en.PU-driven co-expression of BacA\p35UAS.cHa in combination with okrKK107487, spn-AGD5050, grpGD4189 or dapUAS.cdNa, but not the Scer\GAL4en.PU-driven expression of BacA\p35UAS.cHa in combination with trblScer\UAS.cGa or in a DNAlig4169 homozygous background, leads to a longer sustained DNA damage response to ionizing radiation (assessed by phospho-H2Av immunostaining) in the wing disc posterior compartment, as compared to the BacA\p35UAS.cHa single expression or wild-type backgrounds; the longer DNA damage response in both the BacA\p35UAS.cHa, okrKK107487 and BacA\p35UAS.cHa, mei-41GD4258 co-expression backgrounds is suppressed by the additional co-expression of bskK53R.UAS. The basal cell delamination in the wing disc posterior compartment observed upon exposure to ionizing radiation of individuals expressing BacA\p35UAS.cHa under the control of Scer\GAL4en.PU is not suppressed by the co-expression of α-Cat::shgshg.ΔCyt.UASp.
Expression of vtdGL00522 under the control of Scer\GAL4en.PU (together with Dicer-2, for efficient RNAi) leads to a significant increase in aneuploidy in the posterior compartment of the third instar larval wing disc, which is enhanced by co-expressing BacA\p35Scer\UAS.cHa.
Co-expression of BacA\p35Scer\UAS.cHa enhances wing disc phenotypes, with 'undead' cells secreting high levels of wgSH1281 along the A/P margin, in Scer\GAL4bi.PU>Nop60BGL00555 third instar larval wing discs; co-expression of BacA\p35Scer\UAS.cHa also leads to increases in mitotic activity. Co-expression of BacA\p35Scer\UAS.cHa in Scer\GAL4bi.PU>Nop60BGD16822 flies leads to lethality, with rare escapers showing a hyperplastic wing phenotype.
Expression of BacA\p35Scer\UAS.cHa attenuates the protective effect on surrounding cells seen when cell death is initiated through expression of E2f1dsRNA.Scer\UAS.cJa under the control of Scer\GAL4ptc-559.1.
Rare and extremely small homozygous ecdl(3)23 clones can be recovered if cell death is prevented in the clones by expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PI.
Expression of BacA\p35Scer\UAS.cHa strongly suppresses the eye roughness seen when SRm160GE25979 is expressed under the control of Scer\GAL4GMR.PU.
Expression of BacA\p35Scer\UAS.cHa does not suppress the neuroblast loss seen in larval brains expressing Tak1Scer\UAS.cTa under the control of Scer\GAL4wor.PA.
Co-expression of BacA\p35Scer\UAS.cHa with Scer\GAL4phm.PO>Zzzz\ank1Scer\UAS.P\T.cDa using Scer\GAL80ts.αTub84B fails to suppress the developmental arrest phenotype.
Co-expression of BacA\p35Scer\UAS.cHa suppresses the ectopic induction of apoptotic signals and partially suppresses the rough eye phenotype of Scer\GAL4GMR.PU Mcm10dsRNA.Scer\UAS.633-700 animals. However, the inhibition of R7 differentiation resulting from Mcm10dsRNA.Scer\UAS.633-700 expression is not suppressed.
Co-expression of BacA\p35Scer\UAS.cHa suppresses the eye defects caused by expression of DUBAIGD14040 under the control of Scer\GAL4GMR.PFa.
Expression of BacA\p35Scer\UAS.cHa under the simultaneous control of both Scer\GAL4VGlut-OK371 and Scer\GAL4tub.PU rescues the reduced number of LinA-derived neurons seen in ScrC1 AntpNs-rvC3 UbxMX12 triple mutant clones examined at the late third instar larval stage. The reduction in number of glia derived from the triple mutant LinA neuroblast clones is not rescued. Expression of BacA\p35Scer\UAS.cHa under the simultaneous control of both Scer\GAL4VGlut-OK371 and Scer\GAL4tub.PU rescues the reduced number of LinA-derived neurons seen in hthMeis1-P2 mutant clones examined at the late third instar larval stage. The reduction in number of glia derived from the triple mutant LinA neuroblast clones is not rescued. Expression of BacA\p35Scer\UAS.cHa under the simultaneous control of both Scer\GAL4VGlut-OK371 and Scer\GAL4tub.PU partially rescues the defects seen in adult motor neurons of the leg derived from hthMeis1-P2 LinA neuroblast clones. Expression of BacA\p35Scer\UAS.cHa under the simultaneous control of both Scer\GAL4VGlut-OK371 and Scer\GAL4tub.PU partially rescues the defects seen in adult motor neurons of the leg derived from ScrC1 AntpNs-rvC3 UbxMX12 triple mutant LinA neuroblast clones.
Pupal wings containing MARCM clones expressing Hsp83NIG.1242R under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C (co-expressing Dcr-2Scer\UAS.cDa, and BacA\p35Scer\UAS.cHa to inhibit apoptosis) have an increased mitotic index compared to controls. One copy of Rca1IX does not significantly suppress the increased mitotic index seen in pupal wing clones expressing Hsp83NIG.1242R under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C (co-expressed with Dcr-2Scer\UAS.cDa and BacA\p35Scer\UAS.cHa). One copy of Rca12 suppresses the increased mitotic index seen in pupal wing clones expressing Hsp83NIG.1242R under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C (co-expressed with Dcr-2Scer\UAS.cDa and BacA\p35Scer\UAS.cHa).
Animals with lig1 mutant eyes over-expressing BacA\p35Scer\UAS.cHa via eyFLP, Actin-Flp out-Gal4/FRT-mediated mitotic recombination, die as pharate adults except a few escapers that display massively overgrown eye structures. Animals with rin2 Fmr1Δ113M double mutant eyes over-expressing BacA\p35Scer\UAS.cHa via eyFLP, Actin-Flp out-Gal4/FRT-mediated mitotic recombination, die as pharate adults except a few escapers that display massively overgrown eye structures.
Flies co-expressing dmScer\UAS.cZa and BacA\p35Scer\UAS.cHa at 25[o]C under the control of Scer\GAL4Act.PU and Scer\GAL80ts.αTub84B have a longer median lifespan than flies expressing dmScer\UAS.cZa alone.
Co-expression of BacA\p35Scer\UAS.cHa does not suppress the reduction in distance between wing veins 3 and 4 caused by expression of PrpkdsRNA.Scer\UAS.462 under the control of Scer\GAL4salm.EPv. Co-expression of BacA\p35Scer\UAS.cHa does not rescue the small size of clones in the third larval instar wing disc expressing PrpkdsRNA.Scer\UAS.462 under the control of Scer\GAL4Act5C.PU (clones induced at 60 +/- 12 hours after egg laying), although the clones are no longer disaggregated.
Co-expression of BacA\p35Scer\UAS.cHa completely rescues the Scer\GAL4GMR.PS Nf-YBKK107815 rough eye phenotype and the increased eye imaginal disc apoptosis. Co-expression of BacA\p35Scer\UAS.cHa significantly reduces the ectopic/delayed BrdU incorporation and cell division seen in Scer\GAL4GMR.PS Nf-YBKK107815 eye discs. Co-expression of BacA\p35Scer\UAS.cHa does not rescue the lack of R7 photoreceptor differentiation in Scer\GAL4GMR.PS Nf-YBKK107815 eye discs.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2 rescues the axon scaffold phenotypes seen in Df(1)NetABΔ mutant stage 16 embryos. The increased apoptosis seen from stage 14 onwards is also suppressed when BacA\p35Scer\UAS.cHa is expressed under the control of Scer\GAL4AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2. Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4eg-Mz360 significantly suppresses the EW neuron midline crossing phenotypes seen in Df(1)NetABΔ mutant embryos; defects are only seen in 16% of segments, compared to 60% of segments in Df(1)NetABΔ alone. The defects seen in the EG neurons are not rescued. The missing EW neuron phenotype is also unable to be rescued. EG cell death was increased upon expression of BacA\p35Scer\UAS.cHa, but the amount seen is not significantly different from Df(1)NetABΔ. Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2 rescues the negative geotaxis defects seen in Df(1)NetABΔ mutant flies. No rescue is seen when BacA\p35Scer\UAS.cHa is expressed under the control of either Scer\GAL4sim.P3.7 or Scer\GAL4rho.NEE. The mechanical startle-induced locomotor reactivity phenotype is not rescued by expression under the control of any of the three drivers.
BacA\p35Scer\UAS.cHa fails to suppress the semi-lethality seen in Df(1)NetABΔ when expressed under the control of any of Scer\GAL4AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2, Scer\GAL4sca-109-68 or Scer\GAL4sim.P3.7 or Scer\GAL4rho.NEE. Expressing BacA\p35Scer\UAS.cHa using either Scer\GAL4AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2 or Scer\GAL4rho.NEE also fails to suppress the male courtship defects, and expression under the control of Scer\GAL4AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2 is unable to rescue the fertility defects. Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2 is able to partially rescue the flightlessness seen in Df(1)NetABΔ mutant flies.
Expression of BacA\p35Scer\UAS.cHa in Pten117 eye clones (using the MARCM system) efficiently blocks apoptosis and enhanced the overgrowth phenotype. Expression of BacA\p35Scer\UAS.cHa in the dorsal half of the eye under the control of Scer\GAL4mirr-DE blocks apoptosis, results in Pten117 clones overgrowing even more in the dorsal compartment. Blocking apoptosis, through expression of BacA\p35Scer\UAS.cHa in Pten117 clones with diminished slif function (through expression of slifUY681), delays the collapse of the clones but they are absent from adult eyes.
Co-expression of BacA\p35Scer\UAS.cHa rescues the viability of clones in the wing disc expressing P{UAS-DsRed-mir-310+mir-311+mir-312+mir-313.L} (carries mir-310Scer\UAS.cluster.T:Disc\RFP, mir-311Scer\UAS.cluster.T:Disc\RFP, mir-312Scer\UAS.cluster.T:Disc\RFP and mir-313Scer\UAS.cluster.T:Disc\RFP) under the control of Scer\GAL4unspecified. The recovered clones are small, consisting of just one to three cells and appear to be delaminating from the epithelial plane.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4crq.PO partially rescues the embryonic lethality of Df(2L)Pvf2-3/Df(2L)Pvf2-3 mutants as well as the defects in posterior migration of ventrally located hemocytes but does not restore the disrupted passage of hemocytes across the ventral nerve cord or their failure to invade the extended germband of Df(2L)Pvf2-3 mutant embryos.
Larval wing disc cells expressing BacA\p35Scer\UAS.cHa and WScer\UAS.cUa under the control of Scer\GAL4ptc-559.1 display invading cells exclusively in basal planes of the tissue; these cells have cleanly detached and migrate several cell diameters away from the posterior edge of the ptc domain. They display a robust, rounded morphology indicative of healthy cells. The BacA\p35Scer\UAS.cHa-WScer\UAS.cUa invasive cell phenotype is completely suppressed in the presence of msn06946. The BacA\p35Scer\UAS.cHa-WScer\UAS.cUa invasive cell phenotype is completely suppressed by co-expression of bskK53R.Scer\UAS. Knockdown of Nc, through expression of NcNIG.8091R completely suppresses the invasive cell phenotype found upon co-expression of BacA\p35Scer\UAS.cHa-WScer\UAS.cUa under the control of Scer\GAL4ptc-559.1. Reducing Ice levels, through expression of IceNIG.7788R, suppresses the invasive cell phenotype found upon co-expression of BacA\p35Scer\UAS.cHa-WScer\UAS.cUa under the control of Scer\GAL4ptc-559.1.
Scer\GAL4C855a-mediated expression of BacA\p35Scer\UAS.cHa rescues the increase apoptosis and decreased brain size phenotype of aspE3 larvae, but a high degree of brain disorganization is still observed.
Despite suppression of apoptosis through expression of BacA\p35Scer\UAS.cHa, a rough, impaired adult eye phenotype is found in BacA\p35Scer\UAS.cHa/Scer\GAL4GMR.PF; Tak1Scer\UAS.cTa flies, indicating that the Tak1-induced impaired eye phenotype is mainly due to autophagy.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4elav.PU does not rescue the climbing defects of Syx17LL06330/Df(3L)Exel8098 flies.
Expression of BacA\p35Scer\UAS.cHa suppresses the rough eye phenotype and third instar larval eye disc cell death seen when Atg9Scer\UAS.T:Zzzz\FLAG is expressed under the control of Scer\GAL4GMR.PU.
Expression of BacA\p35Scer\UAS.cHa suppresses the reduction in CCAP/bursicon neuron number seen in newly eclosed flies expressing TBPHKK108354 under the control of Scer\GAL4CCAP.PP. The wing inflation defects are also rescued. Expression of BacA\p35Scer\UAS.cHa suppresses the reduction in CCAP/bursicon neuron number seen in newly eclosed flies expressing TBPHScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4CCAP.PP. The wing inflation defects are also rescued.
Co-expression of BacA\p35Scer\UAS.cHa suppresses adult eye phenotypes and apoptosis in third instar eye discs in Scer\GAL4GMR.PU>ClbnScer\UAS.cWa animals.
Expression of BacA\p35Scer\UAS.cHa suppresses the apoptosis seen in wing discs expressing Irk2DN.Scer\UAS under the control of Scer\GAL4Bx-MS1096, although apoptosis is still seen in the periphery of the wing outside of the region of Scer\GAL4Bx-MS1096 expression. The severe wing phenotypes are not suppressed.
Co-expression of E2fScer\UAS.P\T.T:Avic\GFP-EGFP and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en-e16E produces a range of morphological defects in third instar larval wing discs. Whilst some discs appear normal, most display varying degrees of overgrowth in the posterior compartment. Co-expression of E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en-e16E results in a range of morphological defects in third instar larval wing discs, with many discs exhibiting severe overgrowth in the posterior compartment. In approximately 1/3 of discs the posterior compartment is almost absent. Wing discs co-expressing E2fPIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en-e16E appear largely normal.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4sd-SG29.1 at the permissive temperature (25[o]C) induces the development of large tumour-like tissues in the basal region of aPKCts/Df(2R)l4 mutant larval wing discs. Expression of BacA\p35Scer\UAS.cHa does not induce tumors in heterozygous controls.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4c739 suppresses the heat shock stress sensitivity see in Pgam51 mutant flies.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act.PU in homozygous Aos1c06048 clones in the wing disc suppresses the increased cell death seen in these clones, but does not rescue the growth defect, as the double mutant clones are still smaller in size than control clones. The rescued cells appear significantly larger than control cells are are partially extruded from the epithelial layer, leaving only one or two thin membraneous processes attached to the apical epithelial surface.
Co-expression of BacA\p35Scer\UAS.cHa completely suppresses apoptosis in cells in the wing disc expressing HIV-1\VpuScer\UAS.cLa under the control of Scer\GAL4dpp.blk1. The adult wing appears broadly disorganised in these animals, although there is recovery of a full-length L3 vein and partial restoration of issue between veins L2 and L3. An expansion of the space between veins L3 and L4 is seen in the double mutant flies, patches of cells seem to be excluded from the wing epithelium and the overall size of the wing is reduced.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tey-5053A partially suppresses the longitudinal visceral muscle (LVM) founder cell migration defects seen in homozygous binR22 embryos. LVM founder cells regain much of the ability to migrate, however their tracks are less regular. The migrating cell occupy a broader lateral area without being subdivided into a dorsal and a ventral stream and fewer cells reach the very anterior end of the trunk visceral mesoderm (TVM) compared to wild type. Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tey-5053A partially suppresses the longitudinal visceral muscle (LVM) founder cell migration and survival defects seen in homozygous htlAB42 stage 12/13 embryos. LVM founder cells regain much of the ability to migrate, however their tracks towards the anterior of the embryo are less efficient than in wild type with cells seen veering off in dorsal and ventral directions.
The reduced size of Upf126A follicle cell clones is suppressed if the clones are also expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4αTub84B.PL.
Expression of BacA\p35Scer\UAS.cHa partially suppresses the eye pigmentation phenotype seen in 15 day old adults when Hsap\TARDBPScer\UAS.T:Avic\GFP-YFP.cEa is expressed under the control of Scer\GAL4D42 at 25[o]C. Expression of BacA\p35Scer\UAS.cHa partially suppresses the eye pigmentation phenotype seen in 15 day old adults when Hsap\TARDBPA315T.Scer\UAS.T:Avic\GFP-YFP is expressed under the control of Scer\GAL4D42 at 25[o]C.
Co-expression of BacA\p35Scer\UAS.cHa does not rescue the reduced number of cells in the posterior compartment of the wing disc that is seen in animals expressing CycGScer\UAS.P\T.T:Disc\RFP-mRFP under the control of Scer\GAL4en-e16E.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en-e16E in the posterior compartment suppresses the apoptosis seen in Cks85Aex15 third larval instar wing discs.
BacA\p35Scer\UAS.cHa (in the absence of a Scer\GAL4 driver) partially suppresses the lethality and necrotic phenotypes of ade21-6 animals, with suppression being dose-dependent. BacA\p35Scer\UAS.cHa (in the absence of a Scer\GAL4 driver) partially suppresses the lethality and almost completely suppresses the necrotic phenotype of ade24/Df(2L)ED343 and Prat12A19/Df(3R)dsx43 animals.
Expression of BacA\p35Scer\UAS.cHa fails to suppress the dendrite phenotypes seen when Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 is expressed in class IV da neurons under the control of Scer\GAL4ppk.PG.
Co-expression of BacA\p35Scer\UAS.cHa allows recovery of brkScer\UAS.cJa expressing clones under the control of Scer\GAL4Scer\FRT.Act5C in the larval wing disc.
Co-expression of BacA\p35Scer\UAS.cHa does not affect the suppression of ectopic neuroblast formation by p53Scer\UAS.Ex in numbTS4D.Scer\UAS-expressing larval brains (all transgenes under the control of Scer\GAL4insc-Mz1407). Co-expression of BacA\p35Scer\UAS.cHa does not affect the suppression of ectopic neuroblast formation by p53Scer\UAS.Ex (both transgenes under the control of Scer\GAL4insc-Mz1407) in numb15 mutant neuroblast clones.
Co-expression of hpoScer\UAS.cUa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4C855a results in underproliferation of the optic lobe neuroepithelia in larvae.
Co-expression of BacA\p35Scer\UAS.cHa partially rescues the increase in cell death seen in the third larval instar eye disc of animals expressing Hsap\APPAβ42.Scer\UAS.cUa under the control of Scer\GAL4GMR.PU. There is a subtle rescue of the reduced eye size phenotype in the adult, but the neurodegenerative eye phenotype is still present in adult flies.
Constitutive expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4r4 in the fat bodies of Lpine00680/Df(2R)Exel7095 mutants further enhances the lethality observed for Lpine00680/LpinGD14004 animals. The few surviving wandering larvae are characterised by severe lipodystrophy and dramatically oversized and rounded fat body cells. Some of these cells show fragmented nuclei and are indistinguishable from Lpine00680/Df(2R)Exel7095 mutant cells in the absence of BacA\p35Scer\UAS.cHa.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4da.G32 in ctpG0371 mutant animals results in almost complete inhibition of salivary gland degradation, and 96% of animals display intact salivary glands.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4ap-md544 completely rescues the wing notching phenotype of mir-9aJ22/mir-9aE39 animals.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Dll-em212 partially suppresses the growth defects seen in Df(1)btd-Sp1 leg and antennal disc clones generated in second instar larvae.
Expression of BacA\p35Scer\UAS.cHa is unable to suppress the small, rough eye phenotype seen when p53GUS.PB is expressed under the control of Scer\GAL4GMR.PU. However the cell death seen in the posterior half of the p53GUS.PB third instar larval eye disc is strongly inhibited. Co-expression of p53GUS.PB and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4GMR.PU results in loss of all types of photoreceptor neurons.
Expression of BacA\p35Scer\UAS.cHa within qless264 MARCM clones does not prevent mitochondrial stress, but does efficiently rescue the number of cells per qless264 clone.
The abnormal nuclear morphology and apoptosis caused by the overexpression of LamCScer\UAS.cGa under the control of Scer\GAL4ptc-559.1 is suppressed by BacA\p35Scer\UAS.cHa.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4bft-Δ263a-G4 suppresses the loss of interommatidial bristles which is seen in bftΔ263a-G4/bftunspecified flies.
Blocking apoptosis specifically in the caudal visceral mesoderm (along with the presumptive hindgut) through expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4byn-Gal4 suppresses apoptosis in HLH54FΔ598 mutants and restores an almost wild-type size to the caudal visceral mesoderm. However, cells still do not migrate or differentiate into muscle and, instead, remain as unfused cells randomly distributed in the vicinity of the hindgut.
Co-expression of BacA\p35Scer\UAS.cHa causes a clear autonomous reduction in the increase in the number of TUNEL-positive cells seen in the wing discs in larvae expressing Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E at 30[o]C. It also causes a clear non-autonomous rescue of the increase in BrdU incorporation which is seen in the non-expressing compartment in discs expressing Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E at 30[o]C. Co-expression of BacA\p35Scer\UAS.cHa does not rescue the autonomous or the non-autonomous reduction in wing area caused by expression of Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E.
Animals co-expressing BacA\p35Scer\UAS.cHa and Rab5Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4ptc-559.1 show planar polarity defects in the wing.
Expression of BacA\p35Scer\UAS.cHa in the developing eye suppresses the rough eye phenotype induced by expression of Syn2NIG.4905R under the control of Scer\GAL4GMR.PS.
Expression of BacA\p35Scer\UAS.cHa in wing discs expressing GliScer\UAS.cPa under the control of Scer\GAL4ap-md544 blocks cell migration, but overgrowth and ectopic folds in the dorsal wing disc are observed. Expression of BacA\p35Scer\UAS.cHa in wing discs expressing GliFF.Scer\UAS under the control of Scer\GAL4ap-md544 blocks cell migration, and excessive overgrowth and ectopic folds in the dorsal wing disc are observed.
Co-expression of BacA\p35Scer\UAS.cHa rescues eye morphology in flies also expressing rprScer\UAS.T:Scer\GCN4 under the control of Scer\GAL4GMR.PF.
Co-expression of Mmus\wldS.Scer\UAS and BacA\p35Scer\UAS.cHa in ddaC neurons (under the control of Scer\GAL4ppk.PG) results in significantly better protection of dendrites than flies expressing either transgene alone.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub.PU strongly suppresses the apoptosis that is seen in mahj1 or l(2)gl4 clones in the wing disc. Basal extrusion of mutant cells is not fully blocked in these discs.
Co-expression of BacA\p35Scer\UAS.cHa, suppresses the rough eye phenotype induced by the overexpression of TgA.Scer\UAS in the eye imaginal disc under the control of Scer\GAL4GMR.PS.
Co-expression of BacA\p35Scer\UAS.cHa with NcScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 significantly restores bursicon levels in haemolymph and reduces the wing expansion defects regardless of the expression levels of Nc.
The reduction in eye size caused by expression of kibraScer\UAS.P\T.cYa under the control of Scer\GAL4GMR.PF is significantly suppressed by co-expression of BacA\p35Scer\UAS.cHa.
Expression of BacA\p35Scer\UAS.cHa driven by Scer\GAL4hh-Gal4 in posterior wing compartments composed of homozygous wgl-17 mutant cells does not substantially restore normal wing size.
Expression of BacA\p35Scer\UAS.cHa rescues the pupal partial lethality found upon expression of WScer\UAS.cUa under the control of Scer\GAL4Hml.Δ through blocking plasmatocyte apoptosis.
Pupae expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4NP1 undergo midgut histolysis at a similar rate as wild-type animals. Pupae co-expressing decayGD8484 and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4NP1 undergo midgut histolysis at a similar rate as wild-type animals.
Scer\GAL4salm-EPv-driven co-expression of BacA\p35Scer\UAS.cHa with Axud1Scer\UAS.cGa produces epithelial extrusion in the adult wing. The cell cycle abnormalities of the larval wing imaginal disc cells resulting from the Scer\GAL4salm-EPv-driven expression of Axud1Scer\UAS.cGa are not modified by the co-expression of BacA\p35Scer\UAS.cHa.
Co-expression of BacA\p35Scer\UAS.cHa suppresses the increased apoptosis seen in Scer\GAL4nub-AC-62 RYBPScer\UAS.cBa wing discs. Co-expression of BacA\p35Scer\UAS.cHa rescues the small wing size seen in Scer\GAL4unspecified RYBPScer\UAS.cBa wings.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub in clones in the wing disc does not allow the recovery of large homozygous Df(2L)32FP-5 clones in the notum region.
Mutant cells are still extruded from the epithelium in homozygous sds22PB1173 clones in the wing disc that are also expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4toy2 significantly increases the survival of toyhdl homozygous mutants to the adult stage. The head structure defects in these rescued flies, apart from the compound eyes, are also improved. Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4toy2 significantly increases the survival of toyG7.39 homozygous mutants to the adult stage.
Co-expression of BacA\p35Scer\UAS.cHa and ik2dsRNA.Scer\UAS, both under the control of Scer\GAL4sca-P309 generates a short bristle phenotype, as found in Scer\GAL4sca-P309; ik2dsRNA.Scer\UAS mutants.
Normal cell morphology is restored in Cul-4L2-1 MARCM wing disc cell clones that are also expressing BacA\p35Scer\UAS.cHa with the driver Scer\GAL4tub.PU. however, the MARCM clones remain small, with fewer cells than in wild-type twin clones, indicating that cell proliferation is affected by the lack of Cul-4 activity.
The premature amnioserosa dissociation and dorsal closure defects seen in 2X Atg1Scer\UAS.cSa Scer\GAL4Orct2-cald-GAL4 Scer\GAL4Kr.PM embryos are completely suppressed by co-expression of BacA\p35Scer\UAS.cHa.
The increased cell death posterior to the morphogenetic furrow that is seen in Rab11mo homozygous larval eye discs is significantly rescued if the animals are also expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4GMR.PF. The mutant eye phenotype of Rab11mo homozygous adults is partially suppressed by expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4GMR.PF.
Overexpression of BacA\p35Scer\UAS.cHa with Scer\GAL4GMR.PF fails to suppress the cln3Scer\UAS.cTa overexpression phenotype.
48 hours after inducing expression of DadScer\UAS.cTa and BacA\p35Scer\UAS.cHa in the dorsal compartment of the wing disc (under the temperature-regulated control of Scer\GAL4ap-md544 by Scer\GAL80ts.αTub84B), mutant cells are significantly shorter along their apical-basal axis compared to controls. After 56 hours, mutant cells are further shortened, and a deep apical invagination and basal indentation are observed at the border with the dorsal/ventral compartment boundary. Cell volume is conserved in mutant cells. This phenotype is rescued by co-expression of Rho1N19.Scer\UAS, sqhGD1695 or MbsN300.Scer\UAS. Overexpression of DadScer\UAS.cTa and BacA\p35Scer\UAS.cHa in the whole wing disc (under the temperature-regulated control of Scer\GAL4nub-AC-62 by Scer\GAL80ts.αTub84B), results in shorter and apically wider cells. Co-expression of MbsN300.Scer\UAS and BacA\p35Scer\UAS.cHa in the dorsal compartment of the wing disc (under the temperature-regulated control of Scer\GAL4ap-md544 by Scer\GAL80ts.αTub84B) does not alter apical-basal cell length in mutant cells compared with control cells. Co-expression of Rho1N19.Scer\UAS and BacA\p35Scer\UAS.cHa in the dorsal compartment of the wing disc (under the temperature-regulated control of Scer\GAL4ap-md544 by Scer\GAL80ts.αTub84B) results in highly elongated apical-basal cell length in mutant cells compared with control cells. Co-expression of sqhE20E21.Scer\UAS.T:Zzzz\FLAG and BacA\p35Scer\UAS.cHa in the dorsal compartment of the wing disc (under the temperature-regulated control of Scer\GAL4ap-md544 by Scer\GAL80ts.αTub84B) results in shorter mutant cells compared with control cells. Co-expression of sqhGD1695 and BacA\p35Scer\UAS.cHa in the dorsal compartment of the wing disc (under the temperature-regulated control of Scer\GAL4ap-md544 by Scer\GAL80ts.αTub84B) results in highly elongated apical-basal cell length in mutant cells compared with control cells.
Co-expression of BacA\p35Scer\UAS.cHa suppresses the defective eye phenotype caused by expression of Sir2Scer\UAS.cGa under the control of Scer\GAL4GMR.PU.
Co-expression of BacA\p35Scer\UAS.cHa with both lkb1Scer\UAS.cLa and SlnEP2245 considerably suppresses the apoptotic cell death induced by lkb1Scer\UAS.cLa and SlnEP2245 co-expression in the eye (all under the control of Scer\GAL4GMR.PF).
The reduction in eye size caused by expression of AIFΔN.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ey.PH is enhanced by co-expression of BacA\p35Scer\UAS.cHa. The loss of thoracic bristles caused by expression of AIFΔN.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4pnr-MD237 is enhanced by co-expression of BacA\p35Scer\UAS.cHa.
Expression of BacA\p35Scer\UAS.cHa partially suppresses the rough eye phenotype seen in flies expressing Hsap\DEKScer\UAS.cLa under the control of Scer\GAL4GMR.PU.
The reduced recovery of ems3 lateral projection neuron (lPN) clones is rescued to a wild-type recovery rate if the clones are also expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4αTub84B.PL. The average number of cells in these rescued lPN clones corresponds to 71% of the cell number seen in wild-type clones.
Co-expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub in homozygous crolk05205 clones results in more mutant cells being seen in the wing pouch of wing discs from 120 hour larvae (clones induced at 72 hours after egg deposition) compared to homozygous crolk05205 clones induced in an otherwise wild-type background. Wing discs co-expressing both BacA\p35Scer\UAS.cHa and crolScer\UAS.cMa under the control of Scer\GAL4en-e16E show a substantial increase in the size of the posterior compartment in 120 hour larvae. This increase in size is associated with a massive increase in S phase and mitosis. Clones in the wing disc that are co-expressing both BacA\p35Scer\UAS.cHa and crolScer\UAS.cMa under the control of Scer\GAL4Act5C.PP are overgrown and show an increase in BrdU incorporation and in the number of mitotic cells, compared to clones expressing BacA\p35Scer\UAS.cHa alone under the control of Scer\GAL4Act5C.PP. Increased proliferation is also seen in cells adjacent to the clones.
The increased apoptosis that is seen in larval eye discs expressing three copies of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF is suppressed by co-expression of BacA\p35Scer\UAS.cHa. Co-expression of BacA\p35Scer\UAS.cHa and three copies of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF results in eyes and ommatidia that are significantly larger than eyes expressing either dmScer\UAS.cZa or BacA\p35Scer\UAS.cHa alone.
Neurodegeneration in adults with alcΔ12.125 homozygous eyes and antennae (inducing homozygous somatic clones in the eye and antenna with Scer\GAL4ey.PH; Scer\FLP1Scer\UAS.cDa and killing non-mutant cells in the eye with WGMR.PG) is not-suppressed by inhibiting cell death using BacA\p35Scer\UAS.cHa; Scer\GAL4ey.PH.
Co-expression of BacA\p35Scer\UAS.cHa suppresses the reduction in pigment cells and disturbance of the internal architecture of the adult eye seen in animals by expression of three copies of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF.
Co-expression of BacA\p35Scer\UAS.cHa restores the Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 induced loss of rhabdomeres at day 12 post induction. However, the suppressive effect of BacA\p35Scer\UAS.cHa is lost at a later time point.
Co-expression of BacA\p35Scer\UAS.cHa has no effect on the cleft notum phenotype caused by expression of Nf-YAdsRNA.231-399.Scer\UAS.WIZ under the control of Scer\GAL4pnr-MD237.
The persistence of salivary gland tissue that is seen at 24 hours hours after puparium formation when one of BacA\p35Scer\UAS.cHa or Pi3K92EScer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4fkh.PH is significantly increased when both BacA\p35Scer\UAS.cHa and Pi3K92EScer\UAS.T:Hsap\MYC are co-expressed under the control of Scer\GAL4fkh.PH.
Co-expression of BacA\p35Scer\UAS.cHa enhances the proportion of hyperploid cells seen in Scer\GAL4en-e16E, pbldsRNA.Scer\UAS wing discs.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub results in four times more Tctph59 cells being present in clones 72 hours after their induction.
Expression of BacA\p35Scer\UAS.cHa is unable to suppress the eye phenotypes seen in late pupal stage flies expressing HtrA2Scer\UAS.cIa under the control of Scer\GAL4da.G32.
The survival defect of mir-8Δ2/Df(2R)mir-8Δ3 animals is partially suppressed by low-level expression of BacA\p35Scer\UAS.cHa (in the absence of a Scer\GAL4 driver).
Co-expression of BacA\p35Scer\UAS.cHa suppresses the loss of thoracic microchaetae and reduction in scutellum size that is seen in animals expressing one copy of ApplScer\UAS.cTa under the control of Scer\GAL4ap-md544 in a APP-BP1Ex62/+ background.
Cells in the wing disc expressing pzgdsRNA.Scer\UAS under the control of Scer\GAL4en-e16E in a background in which they are also co-expressing BacA\p35Scer\UAS.cHa (to prevent cell death) show strongly reduced DNA replication (as measured by BrdU incorporation) compared to controls, and the number of cells entering mitosis is strongly reduced.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en-e16E increases the contribution of M(3)96Cunspecified/+ cells and decreases the contribution of +/+ cells in the posterior compartment of wing discs containing +/+ clones in a M(3)96Cunspecified/+ background. The more rapid growth of wild-type clones in a M(3)96Cunspecified/+ background (compared to a +/+ background) is eliminated if they are also expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en-e16E.
Blocking cell death in ems3 neuronal clones by expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4αTub84B.PL suppresses the low cell number in these clones.
The border follicle cell migration defects that are seen in flies co-expressing both PvrDN.Scer\UAS and hepAct.Scer\UAS under the control of Scer\GAL4slbo.2.6 are not suppressed by the co-expression of BacA\p35Scer\UAS.cHa.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tey-5053A significantly suppresses the muscle detachment and deterioration seen in stage 16 homozygous mib21 embryos.
Co-expression of BacA\p35Scer\UAS.cHa does not suppress the small, rough eyes with enhanced cell death phenotype of Xbp1Scer\UAS.RB, Scer\GAL4GMR.PF flies.
The partial lethality caused by expression of Atg1Scer\UAS.cSa under the control of Scer\GAL4GMR.PF is rescued by co-expression of BacA\p35Scer\UAS.cHa.
Co-expression of BacA\p35Scer\UAS.cHa delays the death of cells expressing Atg1Scer\UAS.cSa under the control of Scer\GAL4Act5C.PP in clones in the wing disc, although these cells are eventually eliminated from the disc.
The small-eye phenotype is suppressed in flies co-expressing BacA\p35Scer\UAS.cHa with Caf1-180Scer\UAS.cSa under the control of Scer\GAL4ey.PH.
The degradation of the wing disc basement membrane that is seen in animals expressing Tak1Scer\UAS.cMa under the control of Scer\GAL4ptc-559.1 still occurs if BacA\p35Scer\UAS.cHa is co-expressed.
Co-expression of BacA\p35Scer\UAS.cHa suppresses the rough eye phenotype of Scer\GAL4GMR.PF>Hsap\MAPTS396A.S404A.Scer\UAS. Co-expression of BacA\p35Scer\UAS.cHa suppresses the rough eye phenotype of Scer\GAL4GMR.PF>Hsap\MAPTT231A.S235A.Scer\UAS. Co-expression of BacA\p35Scer\UAS.cHa suppresses the rough eye phenotype of Scer\GAL4GMR.PF>Hsap\MAPTS202A.T205A.Scer\UAS. Co-expression of BacA\p35Scer\UAS.cHa suppresses the rough eye phenotype of Scer\GAL4GMR.PF>Hsap\MAPTScer\UAS.cWa.
Co-expression of BacA\p35Scer\UAS.cHa almost completely suppresses the rough eye phenotype caused by expression of ttm50Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF, and in addition, the total number of ommatidia is increased in these flies. Co-expression of BacA\p35Scer\UAS.cHa and ttm50Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4en-e16E results in an increase in nuclear density and an increase in cell proliferation (as assayed by BrdU incorporation) in the imaginal disc.
Co-expression of BacA\p35Scer\UAS.cHa suppresses the male-male courtship seen in males expressing rprScer\UAS.cZa under the control of Scer\GAL4IFa.PT.
Inhibition of cell death, through co-expression of BacA\p35Scer\UAS.cHa and pucScer\UAS.cMa under the control of Scer\GAL4unspecified, only slightly rescues the wing growth defects seen in vgScer\UAS.cKa, Scer\GAL4unspecified flies.
Co-expression of BacA\p35Scer\UAS.cHa suppresses wing notching phenotype which is seen in flies expressing stwlUY823 under the control of Scer\GAL4vg.PM at 21[o]C.
Coexpression of Mkp3GS801 with BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4btl.PS does not suppress the branch integrity and primary branching defects of Mkp3GS801 embryos. Prevention of cell death in Scer\GAL4btl.PS>EgfrDN.Scer\UAS embryos, by coexpression of BacA\p35Scer\UAS.cHa, does not suppress the tracheal branching integrity phenotype.
Coexpression of the BacA\p35Scer\UAS.cHa transgene results in efficient suppression of the wing posture phenotype of Scer\GAL4Mhc.PW>Hsap\PABPN117ala.Scer\UAS flies.
Co-expression of BacA\p35Scer\UAS.cHa allows recovery of larval wing disc clones expressing exScer\UAS.cBa under the control of Scer\GAL4Scer\FRT.Act5C.
Co-expression of BacA\p35Scer\UAS.cHa and casScer\UAS.cKa under the control of Scer\GAL4en-e16E results in loss of U4/U5 neurons (as is seen in embryos expressing casScer\UAS.cKa alone under the control of Scer\GAL4en-e16E).
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4HCH.Hand in Handko mutant embryos prevents ectopic apoptosis. These mutants no longer show reduced lymph gland hematopoietic progenitors and pericardial nephrocytes during the late stage embryonic stage. Larval lethality is not prevented but is delayed. At 18 hours AEL, Scer\GAL4HCH.Hand>BacA\p35Scer\UAS.cHa; Handko larvae start to display an abnormal appearance. At 24 hours AEL, these larvae develop thin hypoplastic hearts and reduced lymph gland hematopoietic progenitors, although this phenotype is less severe than that seen at the same stage in Handko larvae.
Coexpression of BacA\p35Scer\UAS.cHa, which inhibits apoptosis, with kaydsRNA.Scer\UAS under the control of Scer\GAL4GMR.PF results in a more severe phenotype in the eye than when kaydsRNA.Scer\UAS is expressed alone, as ommatidial organization is completely disrupted.
Expressing BacA\p35Scer\UAS.cHa in the wing margin, under the control of Scer\GAL4C96, in a sc10-1 background rescues the stout bristle loss seen in sc10-1 mutants. At the pupal level, BacA\p35Scer\UAS.cHa, expression also rescues the loss of neurons seen in the single mutants. However, this expression also results in the appearance of ectopic neurons at the posterior wing margin, a place where such neurons are not found in wild type. These neurons send out axons which merge with the marginal nerve that runs along the anterior wing margin towards the thorax. Expression of BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4tub, in sensE2 clones fails to rescue the wing margin bristle loss.
The small size of mnmPX1 clones in the eye disc 48 hours after induction is not rescued by expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4hh-Gal4.
Co-expression of BacA\p35Scer\UAS.cHa and rprScer\UAS.cUa in the posterior compartment of the wing disc under the control of Scer\GAL4hh-Gal4 induces dramatic tissue overgrowth. This tissue overgrowth is suppressed by NcΔA8/NcΔA8 but is not suppressed by IceΔ2C8/IceΔ2C8.
Co-expression of E2fScer\UAS.cNa, DpScer\UAS.cDa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4vg.int2.1 results in a wing nicking phenotype, which is partially suppressed by vgnull/+. Co-expression of E2fScer\UAS.cNa, DpScer\UAS.cDa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4vg.int2.1 rescues wing appendage growth in vg83b27 flies; wing blade features such as bristles, wing veins and margin are often seen in the rescued appendages. Co-expression of E2fScer\UAS.cNa, DpScer\UAS.cDa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4vg.int2.1 does not wing blade growth in vgnull flies and only a slight increase in wing hinge growth is seen.
Co-expression of BacA\p35Scer\UAS.cHa with mskScer\UAS.cLa, under the control of Scer\GAL4en-e16E, significantly enhances the adult vein loss observed in BacA\p35Scer\UAS.cHa mutants, resulting in severely distorted wings with enlarged posterior compartments compared with the control anterior compartments (the `J.Lo wing').
Expression of the caspase inhibitor BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4Act5C.PP, in ykiB5 clones only weakly rescues the ykiB5-induced growth defects.
Coexpression of CycEScer\UAS.cLa and BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4en-e16E, in the posterior compartment cells of embryos, blocks the apoptosis of these cells that occurs when CycEScer\UAS.cLa is expressed alone. The posterior compartments of these embryos show a further increase in the number of cells per posterior compartment compared to those expressing only CycEScer\UAS.cLa (77 vs 59). The Scer\GAL4en-e16E>CycEScer\UAS.cLa, BacA\p35Scer\UAS.cHa posterior compartments do not overgrow due to an extreme reduction in average cell size. Coexpression of BacA\p35Scer\UAS.cHa and EgfrDN.Scer\UAS, under the control of Scer\GAL4en-e16E, in the posterior compartment cells of the embryo leads to a 14% reduction in the size of these cells.
Embryos co-expressing BacA\p35Scer\UAS.cHa and Rac1V12.Scer\UAS under the control of Scer\GAL4fkh.PH have abnormally shaped salivary glands that appear to consist of a disorganised cluster of cells, and a salivary gland lumen cannot be detected.
Coexpression of BacA\p35Scer\UAS.cHa with Rab5S43N.Scer\UAS, under the control of Scer\GAL4Act5C.PI in clones of the wing disc show better survival than clones expressing only Rab5S43N.Scer\UAS, but the surviving clones still become extruded from the epithelium, as with clones that only express Rab5S43N.Scer\UAS.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4ey.PH results in a partial suppression of the ventral eye loss in L2/+ flies; 40% of rescued flies have ventral eyes that are more than three-quarters of the wild-type size. The remaining 60% of rescued flies show a range of weaker phenotypes than L2/+ eyes. Coexpression of pucScer\UAS.cMa and BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4ey.PH, does not affect the eye. Coexpression of BacA\p35Scer\UAS.cHa and pucScer\UAS.cMa under the control of Scer\GAL4ey.PH rescues the ventral eye loss of L2/+ flies to a near wild-type eye in 69% of flies and pharate adults; this is a much greater suppression than that seen when either transgene is expressed without the other. Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4ey.PH significantly rescues the loss of the ventral-half of they eye in 32% Scer\GAL4ey.PH>SerBd.Scer\UAS.T:Hsap\MYC flies.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4sca-537.4 partially restores the brain structures that are missing in vnd6 mutant embryos. Notably, descending longitudinal connectives that transverse the tritocerebrum are detectable.
Coexpression of hepAct.Scer\UAS and BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4dpp.blk1, in wing imaginal discs results in the scattering of cells into the adjacent area of the disc from the Scer\GAL4dpp.blk1 expression domain. These cells display extensive actin reorganization and extend actin rich filopodia.
'Undead' cells have been generated in the posterior of the wing disc by simultaneously activating and preventing cell death by coexpression of hidScer\UAS.cYa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4hh-Gal4. At 78 hours AEL these wing discs are similar in size, morphology and the pattern of BrdU incorporation to control discs. However, by 96 hours AEL the discs are significantly smaller than controls; the posterior cells and a subset of anterior cells are arrested in G2 and 'undead' posterior cells are nearly 1.73 larger than anterior cells. By the late wandering stage, the 'undead' posterior cells are similar in size to anterior cells, but their cell cycles have shifted to one similar to younger control wing discs, while arrest in anterior cells is permanent. Cell proliferation of the 'undead' cells continues into pupal development and these cells are unable to differentiate and eventually disintegrate. Neither a tefuatm-6 or lokp6 mutant background can alleviate the overgrowth or anterior cell cycle arrest of wing discs with posterior 'undead' Scer\GAL4hh-Gal4>hidScer\UAS.cYa, BacA\p35Scer\UAS.cHa cells. The cell cycle arrest and undergrowth phenotype of 96 hour wing discs with posterior 'undead' Scer\GAL4hh-Gal4>hidScer\UAS.cYa, BacA\p35Scer\UAS.cHa cells is completely alleviated in both p535A-1-4 and p53-ns backgrounds. 'Undead' Scer\GAL4en-e16E>hidScer\UAS.cYa, BacA\p35Scer\UAS.cHa cells in the posterior of the wing disc show no overgrowth phenotype in a DroncI29 background.
The Pvr1 hemocyte migration phenotype can be suppressed through expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4srp.Hemo. The rescued hemocytes are not only unable to inflitrate the germ band, but also fail to migrate posteriorly from the head along the central nervous system and the dorsal vessel. Pvr1 BacA\p35Scer\UAS.cHa (Scer\GAL4Pxn.PS) mutants show a robust wild-type response to laser ablation, indicating that hemocyte chemotaxis towards wounds is Pvr independent.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4GMR.PF in Nmnat2 photoreceptor clones does not suppress the Nmnat2 phenotype.
Suppression of apoptosis in grhB37 clones, achieved by expression of BacA\p35Scer\UAS.cHa driven by Scer\GAL4elav-C155, results in the continued presence of the neuroblast at 96 hours in over 40% of cases. However, this rescued neuroblast is often larger in size than wild-type clones.
Expression of BacA\p35Scer\UAS.cHa in the posterior compartment of pixL35/pixL17 wing discs, driven by Scer\GAL4en-e16E, increases the area of the posterior compartment relative to control wings that also express BacA\p35Scer\UAS.cHa in the posterior. Expression of BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4en-e16E, in pixL17 wing disc clones increases the frequency of the clones but does not increase their size.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4T155 does not rescue the extrusion of tkvxtr clones from the wing disc.
Co-expression of Su(var)205dsRNA.Sym.Scer\UAS, under the control of Scer\GAL4ey.PH, results in a partial suppression of the reduced eye phenotype seen in Scer\GAL4ey.PH>P{Sym-UAS-Su(var)205} flies.
Expression of BacA\p35Scer\UAS.cHa, driven by Scer\GAL4en-e16E, in a pucA251.1F3/+ background causes tissue overgrowth throughout the posterior compartment of the wing disc in >90% of flies. Disrupted foci within the disc contain excess cells, resulting in abnormal folding of the disc epithelium. Most cells within these foci are not apoptotic. Some discs show extensive areas of overgrowth. In adults, overgrowths in the interior of the wing resemble wing blisters; excess cells appear to be in one compartment as overgrowths project from either the dorsal or the ventral surfaces. When overgrowths arise along the wing margin, excess tissue is readily apparent as an extension of the wing. Similar but less frequent outgrowths occur in posterior regions of adult legs.
The wing defects seen in flies expressing brkScer\UAS.cMa are not due to excessive cell death as preventing cell death by overexpression of BacA\p35Scer\UAS.cHa does not suppress the phenotype. Flies co-expressing brkScer\UAS.cMa and BacA\p35Scer\UAS.cHa, both under the control of Scer\GAL4en-e16E, at 20oC show the same phenotype as flies that express only brkScer\UAS.cMa. When brkScer\UAS.cMa and BacA\p35Scer\UAS.cHa are co-expressed at 250C, again driven by Scer\GAL4en-e16E, there is a slight blistering of the adult wing, indicating that extra wing tissue is present when cell death is prevented. However, the venation patterning defect is still present.
X-ray irradiated wing disc clones expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub.PU in dppd12 mutants contain a mixture of two types of cell: `live' cells, which have not initiated apoptosis due to the irradiation, and `undead' cells (between 20 and 70% of the clone), which have initiated apoptosis, but have failed to complete programmed cell death. No such cells persist outside the clones in which BacA\p35Scer\UAS.cHa is expressed. X-ray irradiated wing disc clones expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub.PU in wgl-17 contain a mixture of two types of cell: `live' cells, which have not initiated apoptosis due to the irradiation, and `undead' cells (between 20 and 70% of the clone), which have initiated apoptosis, but have failed to complete programmed cell death. No such cells persist outside the clones in which BacA\p35Scer\UAS.cHa is expressed. X-ray irradiated wing disc clones expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub.PU in dppd12 wgl-17 mutants contain a mixture of two types of cell: `live' cells, which have not initiated apoptosis due to the irradiation, and `undead' cells (between 20 and 70% of the clone), which have initiated apoptosis, but have failed to complete programmed cell death. No such cells persist outside the clones in which BacA\p35Scer\UAS.cHa is expressed. Unlike BacA\p35Scer\UAS.cHa (Scer\GAL4tub.PU) wing disc clones, neither BacA\p35Scer\UAS.cHa (Scer\GAL4tub.PU) dppd12 nor BacA\p35Scer\UAS.cHa (Scer\GAL4tub.PU) dppd12 wgl-17 clones appear to be associated with extra proliferation or growth after stress (such as X-ray irradiation). Clones expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub.PU in wgl-17 X-ray irradiated wing discs fall into two classes. Clones in the first class do not contain `undead' cells and grow normally. Clones in the second class contain clusters of `undead' cells and overgrow dramatically, forming disorganised masses of tissue that lose the original monolayer organisation characteristic of the wing disc epithelium and instead behave like neoplastic tumors that often bulge out from the main body of the disc. These overproliferating clones tend to fuse, forming large single patches that cover the greater part of the disc, and in many cases (approximately 27%) they seem to constitute the entire disc. BacA\p35Scer\UAS.cHa (Scer\GAL4tub.PU) wgl-17 clones that include `undead' cells proliferate at a much higher rate than the surrounding tissue. However, `undead' cells within these clones divide only rarely, indicating that it is the remaining, live cells within the clones that overgrow. In contrast, no such overgrowth or overproliferation is observed in corresponding control discs containing unstressed clones, or BacA\p35Scer\UAS.cHa (Scer\GAL4tub.PU) dppd12 wgl-17 clones. Co-expression of rprScer\UAS.cZa with BacA\p35Scer\UAS.cHa (both under the control of Scer\GAL4unspecified) in wgl-17 wing disc clones results in most or all of the cells in the clone being `undead'. None of these clones are associated with large, neoplastic outgrowths, and they grow slowly, forming abnormally small clones. FlyBase curator comment: The neoplastic tumours induced by wgl-17 mutant cells blocked for apoptosis (described in FBrf0191458) appear to be due to the loss of l(2)gl function from the mutant chromosome studied and not due to an affect of the wgl-17 mutation (see FBrf0207678).
Expression of BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4ninaE.PHS, partially rescues Arr11 mutant photoreceptor degeneration.
The increased cell death posterior to the morphogenetic furrow that is seen in eye discs expressing marsScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF is suppressed by co-expression of BacA\p35Scer\UAS.cHa.
Expression of inx2Scer\UAS.cBa, under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16, in a BacA\p35Scer\UAS.cHa background (which prevents cell death) results in multilayering and a variation in cell size in the embryos.
Clustering of macrophages, the presence of engulfed apoptotic hemocytes in macrophages and reduction in hemocyte numbers in Pvr1 embryos are all eliminated by BacA\p35Scer\UAS.cHa; Scer\GAL4srp.Hemo. However, hemocyte distribution is still abnormal in these embryos.
Expression of E2fScer\UAS.cNa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4ptc-559.1 in vgnull mutant discs results in an increase in the number of cells in S-phase.
The border follicle cell migration defect seen in egg chambers expressing Rac1N17.Scer\UAS under the control of Scer\GAL4slbo.2.6 is not significantly suppressed by coexpression of BacA\p35Scer\UAS.cHa.
Cell proliferation is increased 2- to 4-fold in the posterior compartment of wing discs coexpressing BacA\p35Scer\UAS.cHa and WScer\UAS.cYa under the control of Scer\GAL4en-e16E. In the adult, this gives rise to wings with an expansion of the posterior compartment associated with several extra tissue folds. Coexpression of thdsRNA.Scer\UAS and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4ptc-559.1 in the third larval instar wing disc does not result in increased cell proliferation in the domain of Scer\GAL4ptc-559.1 expression. The increased cell proliferation seen in the posterior compartment of wing discs and in adult wings coexpressing BacA\p35Scer\UAS.cHa and WScer\UAS.cYa under the control of Scer\GAL4en-e16E is suppressed if they are also coexpressing NcC318S.Scer\UAS. Wing discs coexpressing BacA\p35Scer\UAS.cHa and NcScer\UAS.T:Avic\GFP-EGFP under the control of either Scer\GAL4en-e16E or Scer\GAL4ptc-559.1 show increased cell proliferation in the domain of Scer\GAL4 expression. Wings discs containing clones coexpressing WAla5.Scer\UAS and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PP show increased cell proliferation, with most of the increased proliferation occurring in cells outside the clones.
Co-expression of BacA\p35Scer\UAS.cHa partially restores hemolymph trehalose levels in larvae expressing rprScer\UAS.cZa under the control of Scer\GAL4Akh.PL. Co-expression of BacA\p35Scer\UAS.cHa partially reduces the resistance to starvation-induced death seen in flies expressing rprScer\UAS.cZa under the control of Scer\GAL4Akh.PL.
When 4xdmyc and 2xdmyc clones are made in wing discs that express pucScer\UAS.cMa or BacA\p35Scer\UAS.cHa (driven by Scer\GAL4hh-Gal4) in the posterior compartment, 2xdmyc clones grow significantly more slowly than 4xdmyc clones in the Anterior (control) compartment. This effect is lessened in the posterior compartment. The 4xdmyc clones also grow less well in the posterior compartment.
In ey2 mutant eye-antennal discs that contain clones of cells expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PI, either no endogenous eyes form, or a partial eye forms (as scored by expression of gl) in the ventral region.
In ey2/ey2 BacA\p35Scer\UAS.cHa; Scer\GAL4ey.PH third instar larvae, the eye portion of the eye-antennal disc is replaced by a duplication of the antennal half of the disc. The resulting adults have an extra antenna in place of each eye. This ectopic antenna phenotype is suppressed by eyScer\UAS.ΔPD, which does not rescue eye development. In contrast eyScer\UAS.ΔHD can both rescue eye development and suppress ectopic antenna formation, although this suppression is incompletely penetrant for both aspects of the phenotype.
When BacA\p35Scer\UAS.cHa is driven by Scer\GAL4Dll-981 in the antennal disc of ato1/Df(3R)p13 mutants, only a partial suppression of the ectopic glial cell phenotype is seen. When BacA\p35Scer\UAS.cHa is driven by Scer\GAL4Dll-981 in the antennal disc of lz3/Y mutants, a complete suppression of the ectopic glial cell phenotype is seen.
The small eye phenotype caused by expression of one copy of DDB1dsRNA.650.Scer\UAS under the control of one copy of Scer\GAL4ey.PH is strongly enhanced by co-expression of BacA\p35Scer\UAS.cHa.
In dibunspecified, BacA\p35Scer\UAS.cHa, Scer\GAL4sim.PS embryos, about 10 midline glial cells are seen per neuromere.
BacA\p35Scer\UAS.cHa; Scer\GAL4αTub84B.PL only partially rescues arr2 homozygous somatic clone size in the wing disc: these clones are on average 56% of twin-spot clone size, compared to 17% in the absence of BacA\p35Scer\UAS.cHa. Many mutant clone cells sort out below the disc epithelium. Flow cytometry analysis of these mutant clone cells shows an increase in the fraction of cells in G2 at the expense of cells in G1 compared to wild-type control cells.
Co-expression of BacA\p35Scer\UAS.cHa rescues the reduction in wing size seen in Mmus\Cul1Scer\UAS.cHa Scer\GAL4Bx-MS1096, indicating that Mmus\Cul1Scer\UAS.cHa induces elevated apoptosis. BacA\p35Scer\UAS.cHa expression also suppresses the multiple wing hair phenotype.
When slikScer\UAS.cHa and BacA\p35Scer\UAS.cHa are co-expressed under the control of Scer\GAL4ptc-559.1 an enhancement of the slikScer\UAS.cHa, Scer\GAL4ptc-559.1 overgrowth phenotype is seen. An increase in the number of cells with normal nuclear appearances are found in abnormal outgrowths below the epithelial layer of the wing disc. Adult escapers show overgrowth in the 1st posterior cell of the wing. When slikkd.Scer\UAS and BacA\p35Scer\UAS.cHa are co-expressed under the control of Scer\GAL4ptc-559.1 an enhancement of the slikScer\UAS.cHa, Scer\GAL4ptc-559.1 overgrowth phenotype is seen. An increase in the number of cells with normal nuclear appearances are found in abnormal outgrowths below the epithelial layer of the wing disc. Adult escapers show overgrowth in the 1st posterior cell of the wing.
When slikScer\UAS.cHa and BacA\p35Scer\UAS.cHa are co-expressed under the control of Scer\GAL4ptc-559.1 an enhancement of the slikScer\UAS.cHa, Scer\GAL4ptc-559.1 overgrowth phenotype is seen. An increase in the number of cells with normal nuclear appearances are found in abnormal outgrowths below the epithelial layer of the wing disc. Adult escapers show overgrowth in the 1st posterior cell of the wing. When slikkd.Scer\UAS and BacA\p35Scer\UAS.cHa are co-expressed under the control of Scer\GAL4ptc-559.1 an increase in cell proliferation is seen in the wing disc epithelium and also in the peripodial layer that overlies the disc.
Clone size is substantially increased in fzunspecified fz2unspecified double mutant clones if they are also expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4αTub84B.PL. Clone size is substantially increased in arr2 mutant clones in the wing disc if they are also expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4αTub84B.PL. The size of clones in the wing disc expressing panΔN.Scer\UAS under the control of Scer\GAL4Act5C.PP is increased if they are coexpressing BacA\p35Scer\UAS.cHa.
The addition of BacA\p35Scer\UAS.cHa driven by BacA\p35Scer\UAS.cHa to Nspl-1 somatic clones does not rescue all the eye phenotypes seen in Nspl-1 cells.
Marked clones in the eye disc which are homozygous for scrib1 and are expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PI do not show metastatic behaviour. Marked clones in the eye disc which are homozygous for scrib1 and are co-expressing DpScer\UAS.cDa, E2fScer\UAS.cNa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PI do not show metastatic behaviour.
Coexpression of dltdsRNA.Scer\UAS, driven by Scer\GAL4ptc-559.1, and BacA\p35Scer\UAS.cHa does not alter the number of surviving cells in the wing. However, the dorsal and ventral wing blades are often detached in these mutants and the L3 vein appears broader.
The small head and wing phenotypes, caused by an increase in apoptosis, seen in flies that express hpoScer\UAS.cUa under the control of Scer\GAL4C5 is partially rescued by coexpression of BacA\p35Scer\UAS.cHa.
Co-expression of BacA\p35Scer\UAS.cHa does not rescue the eye phenotype caused by expression of Mmus\Mdm2Scer\UAS.cFa under the control of Scer\GAL4GMR.PF.
The cell death seen in S2 cells expressing rprScer\UAS.cIa under the control of Scer\GAL4Act5C.PHb is suppressed by coexpression of BacA\p35Scer\UAS.cHa. The cell death seen in S2 cells treated with th dsRNA (produced by annealing thcIa and tha.cIa RNA) is slightly suppressed by expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PHb.
BacA\p35Scer\UAS.cHa suppresses the loss of laterally located somatic clones of salmScer\UAS.cdCa; Scer\GAL4Act5C.PP cells in late third instar wing discs. BacA\p35Scer\UAS.cHa suppresses the loss of ventrally located somatic clones of apScer\UAS.cMa; Scer\GAL4Act5C.PP cells in late third instar wing discs. BacA\p35Scer\UAS.cHa suppresses the loss of dorsally located somatic clones of BxScer\UAS.T:Ivir\HA1; Scer\GAL4Act5C.PP cells in late third instar wing discs.
The increase in cell death seen in Scer\GAL469B with nmoc5-1.Scer\UAS embryos is supressed by co-expression of BacA\p35 from BacA\p35Scer\UAS.cHa. The cuticles of these embryos are indistinguishable from those due to expression of BacA\p35 alone (BacA\p35Scer\UAS.cHa; Scer\GAL469B).
The presence of BacA\p35Scer\UAS.cHa supresses the wing ablation phenotype seen in egrScer\UAS.cMa; Scer\GAL4C-765 flies. The presence of BacA\p35Scer\UAS.cHa partially supresses the eye ablation phenotype seen in egrScer\UAS.cMa; Scer\GAL4GMR.PF flies. This supression is dose dependent: 2 copies suppress more effectivley than 1. The presence of BacA\p35Scer\UAS.cHa impedes the elimination of somatic clones expressing egrScer\UAS.cMa under the control of Scer\GAL4Ubx.PdC in the 3rd instar wing disc.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4hh-Gal4 can reduce the cell competition which eliminates Ts(1Lt;2Lt)scS2/+ clones in wild-type wing discs. Co-expression of BacA\p35Scer\UAS.cHa allows the survival of clones expressing brkScer\UAS.cJa under the control of Scer\GAL4Ubx.PdC in the centre of the wing disc, although their proliferation is impaired.
Survival of Df(1)G1 somatic clones in the wing is rescued by BacA\p35 expression in those clones using the 'MARCM' system (clone cells are BacA\p35Scer\UAS.cHa; Scer\GAL4αTub84B.PL, whereas cells outside the clone also carry Scer\GAL80αTub84B.PL). Rescued clones are smaller than wild-type control clones.
Co-expression of BacA\p35Scer\UAS.cHa completely blocks cell death caused by expression of rprScer\UAS.cZa under the control of Scer\GAL4en-e16E. Animals expressing both BacA\p35Scer\UAS.cHa and rprScer\UAS.cZa under the control of Scer\GAL4en-e16E survive to adulthood with largely normal wings.
Homozygous th5 embryos that express BacA\p35Scer\UAS.cHa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 undergo normal morphogenesis until stage 11, after which massive cell death resumes.
The decrease in interface glial cell numbers seen in vnγ4/Df(3L)γ3 is partially suppressed by BacA\p35Scer\UAS.cHa; Scer\GAL4htl.POS, but is unaffected by BacA\p35Scer\UAS.cHa; Scer\GAL4elav.PLu.
Co-expression of BacA\p35Scer\UAS.cHa in clones expressing both capsScer\UAS.cSa and trnScer\UAS.cMa under the control of Scer\GAL4Act5C.PP suppresses the overall loss of ventral clones but ventral clones are still overrepresented approximately 2-fold at the dorsal/ventral boundary.
Co-expression of BacA\p35Scer\UAS.cHa partially rescues the reduced size of wing discs in animals expressing ThorLL.Scer\UAS under the control of Scer\GAL4Bx-MS1096 using the P{UAS-Thor.LL}s transgene. The number of cells in clones in the wing disc expressing ThorLL.Scer\UAS under the control of Scer\GAL4Act5C.PP using the P{UAS-Thor.LL}w is not affected by co-expression of BacA\p35Scer\UAS.cHa. Co-expression of BacA\p35Scer\UAS.cHa greatly increases the number of clones recovered in the wing disc expressing ThorLL.Scer\UAS under the control of Scer\GAL4Act5C.PP using the P{UAS-Thor.LL}s transgene, but only marginally increases the number of cells per clone.
Expression of both Tsc1Scer\UAS.cTa and gigScer\UAS.cTa in the eye under the control of Scer\GAL4ey.PH results in a much smaller eye than normal. Coexpression of BacA\p35Scer\UAS.cHa does not alter this phenotype. Expression of both Tsc1Scer\UAS.cTa and gigScer\UAS.cTa in the wing imaginal disc under the control of Scer\GAL4Act5C.PP results in clones that are smaller than control clones. Coexpression of BacA\p35Scer\UAS.cHa does not alter this phenotype.
Addition of BacA\p35Scer\UAS.cHa to exScer\UAS.cBa, Scer\GAL4hs.2sev flies rescues the phenotypes seen in exScer\UAS.cBa, Scer\GAL4hs.2sev flies alone. Externally the eye is much smoother and in sections most of the cell loss is rescued, although occasional photoreceptor and pigment cell loss is evident. Defects in planar polarity are still prominent.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en-e16E increases the survival of homozygous Ras85DΔC40B clones in the imaginal disc from 2% to 86% (at 72 hours after induction of the clone). The size of the clones is also increased compared to their wild-type sister clones, although there is still a large size difference between them. Co-expressing BacA\p35Scer\UAS.cHa with Ras85DN17.Scer\UAS under the control of Scer\GAL4Act5C.PP completely suppresses the Ras85DN17.Scer\UAS cell death phenotype and increases the number of cells per clone. The doubling time defect is only partially rescued. 40 hours after induction, clones in imaginal discs co-expressing Ras85DN17.Scer\UAS and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PP are 23% smaller than clones expressing BacA\p35Scer\UAS.cHa alone under the control of Scer\GAL4Act5C.PP.
The wing phenotype caused by expression of ppanScer\UAS.cMa under the control of Scer\GAL4Bx-MS1096 is rescued by co-expression of BacA\p35Scer\UAS.cHa.
The locomotor activity defects of flies expressing rprScer\UAS.cZa or WScer\UAS.cZa under the control of Scer\GAL4P2.4.Pdf are partially suppressed by coexpression of BacA\p35Scer\UAS.cHa.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4vg.PU partially suppresses the vg1 wing phenotype. Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4vg.PU does not suppress the vg83b27 wing phenotype.
Scer\GAL4dpp.blk1-mediated expression of Ras85DV12.Scer\UAS causes non-autonomous cell death. Coexpression with BacA\p35Scer\UAS.cHa greatly reduces cell death but animals still die as young adults with deletion of structures, presumably as a consequence of non-autonomous cell death.
77 hour old clones expressing RbfScer\UAS.cDa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en-e16E (induced early in wing disc development) are roughly half the size of control clones.
Co-expression of BacA\p35Scer\UAS.cHa in flies expressing Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF partially restores eye pigmentation.
Cell death caused by Scer\GAL4sca-537.4-mediated expression of Cele\ced-3Scer\UAS.cSa or Hsap\CASP1Scer\UAS.cSa can be blocked by coexpression of BacA\p35Scer\UAS.cHa.
Complementation and Rescue Data
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Mutant
Wild-type
Stocks (8)
Notes on Origin
Discoverer
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Comments
Carried in a plasmid transfected into S2 cells to study its effect on debcl induced apoptosis.
Carried in plasmid "pUAS-p35" and transfected into S2 cells.
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Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
BacA\p35Scer\UAS.cHa
BacA\p35UAS.cHa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Hay
Secondary FlyBase IDs
    References (450)