Allele Dmel\inaD^T1

General Information
Symbol	Dmel\inaD^T1	Species	D. melanogaster
Name		FlyBase ID	FBal0062794
Feature type	allele	Associated gene	Dmel\inaD
Also Known As	inaD¹

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Tsunoda et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:18,744,809..18,744,809 evidence=experimental pr_change=Q271@\|inaD-PA reported_na_change=?811? reported_pr_change=?270@ comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. na_change=C18744809T (Tsunoda et al., 1997)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Nucleotide substitution: ?811?. Amino acid replacement: ?270@. Amber nonsense mutation falls in the second PDZ domain. (Tsunoda et al., 1997)
Cytology
Phenotypic Data
Phenotypic Class
	auditory perception defective \| adult stage (Senthilan et al., 2012) neurophysiology defective (Sanxaridis and Tsunoda, 2010) neurophysiology defective, with inaD^C645S.ninaE (Mishra et al., 2007) neurophysiology defective \| adult stage (Senthilan et al., 2012) neurophysiology defective \| adult stage (with inaD^ninaE.T669A) (Liu et al., 2011) visual behavior defective (Tsunoda et al., 1997) visual behavior defective, with inaD^C645S.ninaE (Mishra et al., 2007) visual behavior defective \| recessive (Scott and Zuker, 1998)
Phenotype Manifest In
	eye photoreceptor cell (Meyer et al., 2006) eye photoreceptor cell, with inaD^C645S.ninaE (Mishra et al., 2007) photoreceptor (Scott and Zuker, 1998) photoreceptor \| adult stage (with inaD^ninaE.T669A) (Liu et al., 2011)
Detailed Description
	Statement Reference Compound action potentials can be evoked by sound in the antennal nerve of mutant flies, but the sound particle velocities required to elicit the response is increased compared to wild type. The displacement response of the antenna over a range of sound particle velocities is linearised, indicating loss of mechanical amplification. (Senthilan et al., 2012) The photoreceptors of newly eclosed flies expressing inaD[ninaE.T669A] in a inaD[T1] mutant background show a slower response to brief (1ms) flashes containing ~75 effectively absorbed photons. Peak amplitude is similar to wild type but the time to peak is delayed and the decay time course is slower. Following 1s light steps of increasing intensity wild type responses consist of an initial peak that rapidly declines to minimum (the "notch"); this "notch" is more pronounced in inaD[T1] inaD[ninaE.T669A] mutant flies. Although the intensity dependence of both the peak and plateau in mutants are indistinguishable from wild-type, the "notch" develops at 2-3 fold lower intensities than in controls. (Liu et al., 2011) The light-triggered translocation of trpl[ninaE.T:Avic\GFP-EGFP] is abolished in inaD[T1] flies. (Meyer et al., 2008) Inhibition of trpl[ninaE.T:Avic\GFP-EGFP] internalisation is observed in inaD[T1] mutant photoreceptors. (Meyer et al., 2006) Mutants have single photon responses that are grossly disorganised, with large defects in amplitude, latency and deactivation. Quantum bumps have amplitudes less than one fifth of those of controls, latencies that are roughly six times greater than wild type and responses that fail to terminate normally, with single photons producing several microbumps. Double mutants with of Cam^unspecified with inaD^T1 show a noisy, continuous response to a single photon of light, and do not produce individual bumps. (Scott and Zuker, 1998) inaD^T1 flies have an abnormal electroretinogram (ERG) phenotype, with severe defects in response to light. Loss of responsiveness to light increases with age. (Tsunoda et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement Reference inaD^su100/inaD^T1 is a suppressor of rhabdomere phenotype of rdgA¹ (Georgiev et al., 2005) inaD^T1/inaD^su1 is a suppressor of rhabdomere phenotype of rdgA¹ (Georgiev et al., 2005) inaD^T1 is a suppressor of rhabdomere phenotype of rdgA¹ (Georgiev et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference The retinal degeneration phenotype of rdgA[1] flies is suppressed by inaD[T1], inaD[T1]/inaD[su1] and inaD[T1]/inaD[su100]. (Georgiev et al., 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	inaD^ttd10 (Meyer et al., 2008)
Rescued by	inaD^T1 is rescued by inaD^ninaE.PM (Mishra et al., 2007)
Partially rescued by	inaD^T1 is partially rescued by inaD^C645S.ninaE (Mishra et al., 2007) inaD^T1 is partially rescued by inaD^{GMR.T:Avic\GFP} (Sanxaridis and Tsunoda, 2010)
Comments	inaD[GMR.T:Avic\GFP] rescues the electroretinogram response of inaD[T1] flies to near wild type. (Sanxaridis and Tsunoda, 2010) Light-raised inaD[ttd10] in trans with inaD[T1] shows partial translocation of trpl[ninaE.T:Avic\GFP-EGFP] from the rhabdomere to the cell body. It should be noted that the trpl[ninaE.T:Avic\GFP-EGFP] translocation defect in homozygous inaD[ttd10] mutants is more severe than in trans with inaD[T1]. inaD[ttd10]/inaD[T1] flies exhibit almost normal internalisation in some photoreceptor cells of each ommatidium whereas the internalisation defect is observed in all photoreceptor cells in homozygous inaD[ttd10] mutants. (Meyer et al., 2008)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	inaD¹ (Scott and Zuker, 1998, Tsunoda et al., 1997, Tsunoda et al., 2001, Baehner et al., 2002, Meyer et al., 2006, Mishra et al., 2007, Chorna-Ornan et al., 2005, Popescu et al., 2006, Meyer et al., 2008, Wes et al., 1999, Sanxaridis and Tsunoda, 2010, Venkatachalam et al., 2010, Peng et al., 2008, Sanxaridis et al., 2007, Senthilan et al., 2012) InaD¹ (Montell, 1998, Georgiev et al., 2005) inaD^T1 (Liu et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 18 )
Research paper	Senthilan et al., 2012, Cell 150(5): 1042--1054 Drosophila auditory organ genes and genetic hearing defects. [FBrf0219321] Liu et al., 2011, Cell 145(7): 1088--1101 The INAD Scaffold Is a Dynamic, Redox-Regulated Modulator of Signaling in the Drosophila Eye. [FBrf0214003] Sanxaridis and Tsunoda, 2010, Fly 4(2): 95--103 A forward genetic screen in Drosophila melanogaster to identify mutations affecting INAD localization in photoreceptor cells. [FBrf0211062] Venkatachalam et al., 2010, J. Neurosci. 30(34): 11337--11345 Dependence on a Retinophilin/Myosin Complex for Stability of PKC and INAD and Termination of Phototransduction. [FBrf0211598] Meyer et al., 2008, Fly 2(1): 36--46 An eGFP-based genetic screen for defects in light-triggered subcelluar translocation of the Drosophila photoreceptor channel TRPL. [FBrf0210490] Peng et al., 2008, J. Neurochem. 104(6): 1526--1535 Anchoring TRP to the INAD macromolecular complex requires the last 14 residues in its carboxyl terminus. [FBrf0204232] Mishra et al., 2007, Cell 131(1): 80--92 Dynamic scaffolding in a G protein-coupled signaling system. [FBrf0202291] Sanxaridis et al., 2007, Mol. Cell. Neurosci. 36(1): 36--46 Light-induced recruitment of INAD-signaling complexes to detergent-resistant lipid rafts in Drosophila photoreceptors. [FBrf0201518] Meyer et al., 2006, J. Cell Sci. 119(Pt 12): 2592--2603 Subcellular translocation of the eGFP-tagged TRPL channel in Drosophila photoreceptors requires activation of the phototransduction cascade. [FBrf0193744] Popescu et al., 2006, J. Neurosci. 26(33): 8570--8577 Scaffolding protein INAD regulates deactivation of vision by promoting phosphorylation of transient receptor potential by eye protein kinase C in Drosophila. [FBrf0192668] Chorna-Ornan et al., 2005, J. Cell Biol. 171(1): 143--152 Light-regulated interaction of Dmoesin with TRP and TRPL channels is required for maintenance of photoreceptors. [FBrf0190927] Georgiev et al., 2005, J. Cell Sci. 118(Pt 7): 1373--1384 Functional INAD complexes are required to mediate degeneration in photoreceptors of the Drosophila rdgA mutant. [FBrf0184068] Baehner et al., 2002, Neuron 34(1): 83--93 Light-regulated subcellular translocation of Drosophila TRPL channels induces long-term adaptation and modifies the light-induced current. [FBrf0147176] Tsunoda et al., 2001, J. Neurosci. 21(1): 150--158 Independent anchoring and assembly mechanisms of INAD signaling complexes in Drosophila photoreceptors. [FBrf0132378] Wes et al., 1999, Nat. Neurosci. 2(5): 447--453 Termination of phototransduction requires binding of the NINAC myosin III and the PDZ protein INAD. [FBrf0108298] Scott and Zuker, 1998, Nature 395(6704): 805--808 Assembly of the Drosophila phototransduction cascade into a signalling complex shapes elementary responses. [FBrf0105321] Tsunoda et al., 1997, Nature 388(6639): 243--249 A multivalent PDZ-domain protein assembles signalling complexes in a G-protein-coupled cascade. [FBrf0096480]
Review	Montell, 1998, Curr. Opin. Neurobiol. 8(3): 389--397 TRP trapped in fly signaling web. [FBrf0103331]

Allele Dmel\inaDT1

Allele Dmel\inaD^T1