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General Information
Symbol
Dmel\osa308
Species
D. melanogaster
Name
FlyBase ID
FBal0062861
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
osaeld308
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

At 50 hours after clone induction osa308 mutant clones contain a single type II NB, indistinguishable from controls. However the number of immature Intermediate Neural Progenitors (imINPs) is significantly increased compared to controls. By seventy five hours after clone induction, multiple type II NB-like cells are seen, compared to only one in control clones, and these are several cell diameters away from the primary NB. The number of imINPs is no longer significantly different from controls and there is no significant difference in the numbers of mature Intermediate Neural Progenitors (mINPs) numbers

Somatic clones of osa308 tissue induced in a non-Minute background proliferate poorly in the wing disc, and can not be recovered in the adult wing. Growth of osa308 cells is rescued in a Minute[+] background. A fraction of these cells localise to the basal side of the wing blade epithelium, are extruded basally, and undergo cell death. These animals die as pupae.

osa308 homozygous mutant clones generated in the entire wing blade result in wings that are very reduced in size, lacking all the veins and most structures of the wing margin. This phenotype is cell-autonomous.

osa308 homozygous mutant clones generated in the central region of the wing blade results in basal extrusion of some wing blade epithelial cells and cell death. Adult wings develop no distal veins, and have ectopic hairs and sensory organs. This phenotype is cell-autonomous.

osa308 homozygous mutant clones generated in the tarsal segments of the developing leg results in fusion of the adult tarsal segments, and loss of the distal-most elements.

In osa308 clones in the eye disc a reduced number of photoreceptors differentiate.

Dominant held out wing phenotype. Clones lead to scars and loss of retinal differentiation.

Homozygous mutant somatic clones can induce leg duplications an the ventral compartment of the leg.

Mosaic clones in the eye disc are rarely recovered, existing clones are associated with scars and within the clone there are fewer than normal photoreceptor clusters and within each cluster there are fewer than normal neuronal cells. Clones that include the posterior margin are frequently much larger than internal clones. Clones induced on the wing disc produced early in development transforms the posterior notum into a partial second wing. The wings have reversed anterior-posterior polarity. Clones induced later in development are associated with ectopic wing margin bristles near the wing margin restricted to the dorsal surface of the anterior compartment. Embryos derived from germline clones show severe defects in the cuticle pattern with many denticle belts either missing, fused or otherwise abnormal. Provision of a paternal wild type copy of osa fails to rescue the mutants.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Enhancer of
Statement
Reference

osa[+]/osa308 is an enhancer of wing margin phenotype of Bx1

osa308 is an enhancer of eye phenotype of dppd-blk

NOT Enhancer of
Statement
Reference

osa[+]/osa308 is a non-enhancer of leg phenotype of e(y)34

Suppressor of
Statement
Reference

osa[+]/osa308 is a suppressor of eye phenotype of Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU

osa[+]/osa308 is a suppressor of eye phenotype of Hsap\MECP2Δ166.UAS, Scer\GAL4GMR.PU

osa[+]/osa308 is a suppressor | partially of wing margin phenotype of BxhdpR590

osa[+]/osa308 is a suppressor of eye phenotype of roDOM

osa308 is a suppressor of eye phenotype of roDOM

NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

The formation of ectopic wing veins observed in adult flies expressing Marcal1Scer\UAS.cBa under the control of Scer\GAL4tub.PU is ameliorated by combination with a single copy of osa308.

One copy of osa308 enhances the supernumerary neuroblast phenotype seen in bratDG19310/brat11 mutants.

Stage 16 osa308/+ ; akirinKG01343/+ double heterozygous embryos show disruption of the somatic muscle pattern (42% of embryos have missing, misattached or duplicated muscles in at least 2 hemisegments).

The ectopic wing vein phenotype observed in homozygous bs2 mutant wings is suppressed in animals doubly heterozygous for osa308 and brm2.

net1, px72 double mutants develop ectopic wing veins in the L2/L3 and L4/L5 intervein regions. This phenotype is suppressed in flies double heterozygous for osa308 and brm2.

The loss of wing vein observed by overexpression of Ras85DN17.UAS under the control of Scer\GAL4MD-638 is enhanced by osa308/+.

The effects on wing shape and size in animals expressing Ras85DV12.UAS under the control of Scer\GAL4MD-638 are partially rescued in a osa308/+ background, although these wings still show ectopic vein differentiation.

The formation of ectopic wing veins in flies expressing rlSem.cUa.UAS under the control of Scer\GAL4salm-459.2 is weakly enhanced in a osa308/+ mutant background.

Bap170Δ65 females with osa308 polybromoΔ86 germ lines clones are able to differentiate oocytes.

The severity of the Bx1/+ wing scalloping phenotype is enhanced by one copy of osa308.

Strong suppressor of roDOM.

groE48/osa308 transheterozygous adults have notum to wing transformations.

The addition of osa308 strongly enhances the wing phenotypes seen in brmK804R.Scer\UAS.T:Ivir\HA1/Scer\GAL4bi-omb-Gal4 flies i.e. loss of the distal wing margin, formation of ectopic campaniform sensillae and wing margin bristles and disruptions of wing vein morphology. osa308 also enhances the wing margin loss phenotype seen in brmK804R.Scer\UAS.T:Ivir\HA1/Scer\GAL4vg.PM flies. The addition of osa308 enhances the ectopic wing margin bristle and wing vein phenotypes caused by Scer\GAL4bi-omb-Gal4 driven expression of en::osaosaRD.Scer\UAS. The growth phenotype seen in mor1 somatic clones is partially rescued by osa308.

Df(3R)Espl22, osa308 double mutant clones in the eye show excess neuronal differentiation.

Xenogenetic Interactions
Statement
Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1Scer\UAS.cBa under the control of Scer\GAL4Bx-MS1096 is ameliorated by combination with a single copy of osa308.

Complementation and Rescue Data
Comments
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Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (19)