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General Information
Symbol
Dmel\ci76.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0062923
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Ci-75, UAS-Cirep, UAS-ci76
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of ci that lacks amino acid residues C-terminal to Asn-703.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

ci94 embryos expressing ci76.Scer\UAS under the control of Scer\GAL4ptc-559.1 have a cuticular phenotype close to that of ciCe-2 embryos. Denticle diversity is still seen. Type 4 denticles are still present, although they are sometimes reduced in some segments.

When driven by Scer\GAL4c591 a phenotype is seen that is very similar to that of hh loss of function mutations. The ocelli are eliminated and ectopic frons cuticle appears in the medial region.

Expression of ci76.Scer\UAS driven by Scer\GAL4dpp.blk1 causes the partial loss of L3-L4 intervein.

Cells expressing ci76.Scer\UAS under the control of Scer\GAL4αTub84B.PP at the posterior margin of the lamina furrow fail to enter S phase. Where clones of cells expressing ci76.Scer\UAS under the control of Scer\GAL4αTub84B.PP cross the lamina furrow, S phase lamina neuron precursors (LPCs) are absent, while S phase LPCs are seen immediately outside of the clone. No defects are seen in other proliferation zones such as the outer proliferation centre or inner proliferation centre when they contain clones of cells expressing ci76.Scer\UAS under the control of Scer\GAL4αTub84B.PP.

Scer\GAL4ptc-559.1-mediated expression at 16oC allows recovery of some escapers, these exhibit an abnormal wing phenotype. Scer\GAL4en-e16E-mediated expression at 16oC also allows recovery of some escapers that exhibit almost complete loss of wings, severely disrupted legs and reduced thorax.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT suppressed by
Statement
Reference
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of ci76.Scer\UAS or significantly suppresses the withdrawal responses to a 38[o]C probe (without pre-exposure to UV) seen in early third instar larvae with TkR99DScer\UAS.T:Avic\GFP-EGFP over-expressed by Scer\GAL4ppk.1.9.

ci76.Scer\UAS enhances the eye overgrowth phenotype seen when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PH. Eye overgrowth is seen with 75% penetrance.

hydK7.19 clones in the eye disc which are also expressing ci76.Scer\UAS under the control of Scer\GAL4αTub84B.PL sometimes result in ectopic photoreceptor differentiation in the tissue surrounding the clone. hydK7.19 hhrJ413 double mutant clones in the eye which are also expressing ci76.Scer\UAS under the control of Scer\GAL4αTub84B.PL do not show overgrowth.

The wing vein phenotype caused by expression of ci76.Scer\UAS driven by Scer\GAL4dpp.blk1 cannot be rescued by Scer\GAL4 driven expression of knScer\UAS.cVa.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Carried in plasmid "Ci76", coexpressed in S2 cells with Scer\GAL4 and localisation of the protein is assayed.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
ci76.Scer\UAS
ci76.UAS
Name Synonyms
Secondary FlyBase IDs
    References (14)