FlyBase Allele Report: Dmel\ci[76.UAS]

General Information

Symbol

Dmel\ci^76.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0062923

Feature type

allele

Associated gene

Dmel\ci

Associated Insertion(s)

Carried in Construct

P{UAS-ci.76}

Also Known As

UAS-ci⁷⁶, UAS-Ci-75, UAS-Ci^rep

Key Links

Transgenic product class

inframe_deletion

(Aza-Blanc et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Aza-Blanc et al., 1997)

Mutagen

in vitro construct

(Aza-Blanc et al., 1997)

Progenitor genotype

Carried in construct

P{UAS-ci.76}

Cytology

Description

UASt regulatory sequences drive expression of ci that lacks amino acid residues C-terminal to Asn-703.

(Aza-Blanc et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

DOES NOT ameliorate cancer

modeled by Hipk^UAS.Tag:HA

(Blaquiere et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | somatic clone, with Scer\GAL4^αTub84B.PP

(Huang and Kunes, 1998)

lethal, with Scer\GAL4^en-e16E

(Aza-Blanc et al., 1997)

lethal, with Scer\GAL4^ptc-559.1

(Aza-Blanc et al., 1997)

visible, with Scer\GAL4^c591

(Amin et al., 1999)

visible, with Scer\GAL4^dpp.blk1

(Vervoort et al., 1999)

Phenotype Manifest In

adult head capsule | dorsal, with Scer\GAL4^c591

(Amin et al., 1999)

embryonic/first instar larval cuticle, with Scer\GAL4^ptc-559.1, ci⁹⁴

(Gallet et al., 2000)

first posterior wing cell, with Scer\GAL4^dpp.blk1

(Vervoort et al., 1999)

lamina, with Scer\GAL4^αTub84B.PP

(Huang and Kunes, 1998)

lamina & neuron | precursor, with Scer\GAL4^αTub84B.PP

(Huang and Kunes, 1998)

leg, with Scer\GAL4^en-e16E

(Aza-Blanc et al., 1997)

ocellus, with Scer\GAL4^c591

(Amin et al., 1999)

thorax, with Scer\GAL4^en-e16E

(Aza-Blanc et al., 1997)

ventral denticle belt, with Scer\GAL4^ptc-559.1, ci⁹⁴

(Gallet et al., 2000)

wing, with Scer\GAL4^en-e16E

(Aza-Blanc et al., 1997)

wing, with Scer\GAL4^ptc-559.1

(Aza-Blanc et al., 1997)

Detailed Description

Statement

Reference

ci⁹⁴ embryos expressing ci^76.Scer\UAS under the control of Scer\GAL4^ptc-559.1 have a cuticular phenotype close to that of ci^Ce-2 embryos. Denticle diversity is still seen. Type 4 denticles are still present, although they are sometimes reduced in some segments.

(Gallet et al., 2000)

When driven by Scer\GAL4^c591 a phenotype is seen that is very similar to that of hh loss of function mutations. The ocelli are eliminated and ectopic frons cuticle appears in the medial region.

(Amin et al., 1999)

Expression of ci^76.Scer\UAS driven by Scer\GAL4^dpp.blk1 causes the partial loss of L3-L4 intervein.

(Vervoort et al., 1999)

Cells expressing ci^76.Scer\UAS under the control of Scer\GAL4^αTub84B.PP at the posterior margin of the lamina furrow fail to enter S phase. Where clones of cells expressing ci^76.Scer\UAS under the control of Scer\GAL4^αTub84B.PP cross the lamina furrow, S phase lamina neuron precursors (LPCs) are absent, while S phase LPCs are seen immediately outside of the clone. No defects are seen in other proliferation zones such as the outer proliferation centre or inner proliferation centre when they contain clones of cells expressing ci^76.Scer\UAS under the control of Scer\GAL4^αTub84B.PP.

(Huang and Kunes, 1998)

Scer\GAL4^ptc-559.1-mediated expression at 16^oC allows recovery of some escapers, these exhibit an abnormal wing phenotype. Scer\GAL4^en-e16E-mediated expression at 16^oC also allows recovery of some escapers that exhibit almost complete loss of wings, severely disrupted legs and reduced thorax.

(Aza-Blanc et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ci^76.UAS, Scer\GAL4^dpp.blk1 is a suppressor | partially of neoplasia phenotype of Hipk^UAS.Tag:HA, Scer\GAL4^dpp.blk1

(Blaquiere et al., 2018)

ci^76.UAS, Scer\GAL4^dpp.blk1 is a suppressor | partially of abnormal cell migration | larval stage phenotype of Hipk^UAS.Tag:HA, Scer\GAL4^dpp.blk1

(Blaquiere et al., 2018)

ci^76.UAS/Scer\GAL4^ppk.1.9 is a suppressor | partially of abnormal pain response | early third instar larval stage | heat sensitive phenotype of Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP

(Im et al., 2015)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^dpp.blk1, ci^76.UAS has first posterior wing cell phenotype, non-suppressible by kn^UAS.cVa/Scer\GAL4^dpp.blk1

(Vervoort et al., 1999)

Suppressor of

Statement

Reference

ci^76.UAS, Scer\GAL4^dpp.blk1 is a suppressor | partially of wing disc phenotype of Hipk^UAS.Tag:HA, Scer\GAL4^dpp.blk1

(Blaquiere et al., 2018)

Other

Statement

Reference

Scer\GAL4^αTub84B.PL, ci^76.UAS, hyd^K7.19 has eye disc | somatic clone phenotype

(Lee et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of ci^76.Scer\UAS or significantly suppresses the withdrawal responses to a 38[o]C probe (without pre-exposure to UV) seen in early third instar larvae with TkR99D^{Scer\UAS.T:Avic\GFP-EGFP} over-expressed by Scer\GAL4^ppk.1.9.

(Im et al., 2015)

ci^76.Scer\UAS enhances the eye overgrowth phenotype seen when Dl^Scer\UAS.cDa is expressed under the control of Scer\GAL4^ey.PH. Eye overgrowth is seen with 75% penetrance.

(Da Ros et al., 2013)

hyd^K7.19 clones in the eye disc which are also expressing ci^76.Scer\UAS under the control of Scer\GAL4^αTub84B.PL sometimes result in ectopic photoreceptor differentiation in the tissue surrounding the clone. hyd^K7.19 hh^rJ413 double mutant clones in the eye which are also expressing ci^76.Scer\UAS under the control of Scer\GAL4^αTub84B.PL do not show overgrowth.

(Lee et al., 2002)

The wing vein phenotype caused by expression of ci^76.Scer\UAS driven by Scer\GAL4^dpp.blk1 cannot be rescued by Scer\GAL4 driven expression of kn^Scer\UAS.cVa.

(Vervoort et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in plasmid "Ci76", coexpressed in S2 cells with Scer\GAL4 and localisation of the protein is assayed.

(Aza-Blanc et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ci-76

(Vervoort et al., 1999)

DN-Ci

(Huang and Kunes, 1998)

ci^76.Scer\UAS

ci^76.UAS

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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