Allele Dmel\Hsp70Bc^+t14

General Information
Symbol	Dmel\Hsp70Bc^+t14	Species	D. melanogaster
Name		FlyBase ID	FBal0063183
Feature type	allele	Associated gene	Dmel\Hsp70Bc

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - genomic fragment
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - genomic fragment (Welte et al., 1993, Krebs et al., 1998, Krebs and Feder, 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References rescue fragment 3R:8,325,423..8,339,292 comment=Position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=HindIII-HindIII_rfrag (Welte et al., 1993)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: 14kb HindIII fragment of plasmid G3 carrying three Hsp70 genes is cloned between two direct FRT sites. (Welte et al., 1993)
Carried in construct	P{FRT(w^hs,3Xwt70).A} (Silbermann and Tatar, 2000, Bettencourt et al., 1999, Tatar et al., 1997, Bettencourt and Feder, 2001, Minois et al., 2001, Krebs et al., 1998, Krebs and Feder, 1998, Minois, 2001, Welte et al., 1993, Krebs and Feder, 1997, Feder et al., 1996, Klose et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	aging defective \| adult stage \| male limited \| heat sensitive (Minois et al., 2001)
Phenotype Manifest In

Detailed Description
	Statement Reference Overexpression of Hsp70Bb[+t14] and Hsp70Bc[+t14] after heat shock (in animals carrying P{FRT(w[hs],3Xwt70).A}) does not confer enhanced protection against hyperthermia, but has deleterious effects on parameters of larval locomotory behaviour. (Klose et al., 2005) A 30 minute exposure at 36^oC in females carrying extra copies of Hsp70 (female carrying P{>w^hs,3Xwt70>A}) results in a decrease in the proportion of eggs that hatch compared to control heat shocked flies. A 60 minute exposure to 36^oC results in a decrease in the proportion of eggs that hatch both in control heat shocked flies and in flies carrying extra copies of Hsp70 (female carrying P{>w^hs,3Xwt70>A}). (Silbermann and Tatar, 2000) First instar larvae carrying P{>w^hs,3Xwt70>A} are more thermotolerant than control larvae in the absence of pretreatment or with a 10 minute pretreatment at 36^oC before heat shock. Control larvae are more thermotolerant than larvae carrying P{>w^hs,3Xwt70>A} if they receive a 30 minute pretreatment before heat shock. (Krebs and Feder, 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Hsp70Bb^+t14, Hsp70Bc^+t14 has chemical resistant phenotype (Minois, 2001, Minois, 2001)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Flies carrying P{>w^hs,3Xwt70>A} are more resistant to paraquat than control lines. Flies carrying P{>w^hs,3Xwt70>A} and subjected to heat shock do not show enhanced stress resistance compared to control lines subjected to heat shock. (Minois, 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsp70Bc^+t14
Name Synonym
Secondary FlyBase IDs

References ( 12 )
Research paper	Klose et al., 2005, J. Neurophysiol. 94(5): 3563--3572 Heat shock-mediated thermoprotection of larval locomotion compromised by ubiquitous overexpression of Hsp70 in Drosophila melanogaster. [FBrf0191037] Bettencourt and Feder, 2001, Mol. Biol. Evol. 18(7): 1272--1282 Hsp70 duplication in the Drosophila melanogaster species group: how and when did two become five? [FBrf0137034] Minois, 2001, J. Insect Physiol. 47(9): 1007--1012 Resistance to stress as a function of age in transgenic Drosophila melanogaster overexpressing Hsp70. [FBrf0137277] Minois et al., 2001, Exp. Gerontol. 36(7): 1137--1153 Locomotor activity as a function of age and life span in Drosophila melanogaster overexpressing hsp70. [FBrf0136881] Silbermann and Tatar, 2000, Evolution 54(6): 2038--2045 Reproductive costs of heat shock protein in transgenic Drosophila melanogaster. [FBrf0134612] Bettencourt et al., 1999, Evolution 53(2): 484--492 Experimental evolution of Hsp70 expression and thermotolerance in Drosophila melanogaster. [FBrf0108450] Krebs and Feder, 1998, J. Insect Physiol. 44(11): 1091--1101 Hsp70 and larval thermotolerance in Drosophila melanogaster: How much is enough and when is more too much? [FBrf0105401] Krebs et al., 1998, Evolution 52(3): 841--847 Heritability of expression of the 70KD heat-shock protein in Drosophila melanogaster and its relevance to the evolution of thermotolerance. [FBrf0103438] Krebs and Feder, 1997, Cell Stress Chaperones 2(1): 60--71 Deleterious consequences of Hsp70 overexpression in Drosophila melanogaster larvae. [FBrf0093550] Feder et al., 1996, J. Exp. Biol. 199(8): 1837--1844 Effect of engineering Hsp70 copy number on Hsp70 expression and tolerance of ecologically relevant heat shock in larvae and pupae of Drosophila melanogaster. [FBrf0089647] Welte et al., 1993, Curr. Biol. 3(12): 842--853 A new method for manipulating transgenes: engineering heat tolerance in a complex, multicellular organism. [FBrf0097782]
Letter	Tatar et al., 1997, Nature 390(6655): 30 Chaperoning extended life. [FBrf0099657]

Allele Dmel\Hsp70Bc+t14

Allele Dmel\Hsp70Bc^+t14