Allele Dmel\E(spl)m8-HLH^Scer\UAS.cGa

General Information
Symbol	Dmel\E(spl)m8-HLH^Scer\UAS.cGa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Giebel	FlyBase ID	FBal0063456
Feature type	allele	Associated gene	Dmel\E(spl)m8-HLH

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Giebel and Campos-Ortega, 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Full length wild type cDNA is cloned into the pUAST vector. (Giebel and Campos-Ortega, 1997)
Carried in construct	P{UAS-E(spl)} (Giebel, 1999, Nagel et al., 1999, Kumar and Moses, 2001, Kumar and Moses, 2001, Giebel and Campos-Ortega, 1997, Mohit et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	adult abdomen & macrochaeta, with Scer\GAL4^sca-537.4 (Giebel and Campos-Ortega, 1997) adult head & macrochaeta, with Scer\GAL4^sca-537.4 (Giebel and Campos-Ortega, 1997) embryonic/larval somatic muscle, with Scer\GAL4^da.G32 (Giebel, 1999) embryonic/larval somatic muscle, with Scer\GAL4^how-24B (Giebel, 1999) morphogenetic furrow \| ectopic, with Scer\GAL4^dpp.blk1 (Kumar and Moses, 2001) muscle founder cell, with Scer\GAL4^da.G32 (Giebel, 1999) muscle founder cell, with Scer\GAL4^how-24B (Giebel, 1999) peripheral nervous system, with Scer\GAL4^da.G32 (Giebel, 1999) scutum & macrochaeta & adult, with Scer\GAL4^sca-537.4 (Giebel and Campos-Ortega, 1997)
Detailed Description
	Statement Reference Expression of E(spl)^Scer\UAS.cGa under the control of Scer\GAL4^ey.PB has no effect on either eye or antenna disc development. (Kumar and Moses, 2001) Expression of E(spl)^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 results in ectopic furrow formation in the eye disc. (Kumar and Moses, 2001) Expression of E(spl)^Scer\UAS.cGa driven by Scer\GAL4^da.G32 or Scer\GAL4^how-24B have a reduced number of Kr positive muscle founder cells and a corresponding defect in the number of differentiated muscle cells. A suppression of peripheral nervous system structures is also seen when driven by Scer\GAL4^da.G32. (Giebel, 1999) No effect on eye morphogenesis is seen when E(spl)^Scer\UAS.cGa is expressed in the eye under the control of Scer\GAL4^h-1J3. (Nagel et al., 1999) Scer\GAL4^sca-537.4-mediated expression suppresses development of bristles on the notum, abdomen and head. (Giebel and Campos-Ortega, 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement Reference E(spl)m8-HLH^Scer\UAS.cGa/Scer\GAL4^{Ubx-lac1-Gal4} is a non-enhancer of visible \| homeotic phenotype of Ubx^lac1-GAL4 (Mohit et al., 2006)
Phenotype Manifest In
NOT Enhancer of
Statement Reference E(spl)m8-HLH^Scer\UAS.cGa/Scer\GAL4^h-1J3 is a non-enhancer of eye phenotype of N^spl-1 (Nagel et al., 1999) E(spl)m8-HLH^Scer\UAS.cGa/Scer\GAL4^{Ubx-lac1-Gal4} is a non-enhancer of haltere phenotype of Ubx^lac1-GAL4 (Mohit et al., 2006) E(spl)m8-HLH^Scer\UAS.cGa/Scer\GAL4^{Ubx-lac1-Gal4} is a non-enhancer of wing hair \| ectopic phenotype of Ubx^lac1-GAL4 (Mohit et al., 2006) E(spl)m8-HLH^Scer\UAS.cGa/Scer\GAL4^{Ubx-lac1-Gal4} is a non-enhancer of wing margin bristle \| ectopic phenotype of Ubx^lac1-GAL4 (Mohit et al., 2006)
Suppressor of
Statement Reference E(spl)m8-HLH^Scer\UAS.cGa, Scer\GAL4^455.2 is a suppressor of scutellar bristle \| ectopic phenotype of Scer\GAL4^455.2, l(1)sc^Scer\UAS.cHa (Giebel and Campos-Ortega, 1997)
Additional Comments
Genetic Interactions
Statement Reference Expression of E(spl)^Scer\UAS.cGa in a Ubx^lac1-GAL4 background (the transgene expression is driven by the P{GawB} in the insertion that generates the Ubx^lac1-GAL4 allele) does not enhance the haltere phenotype of Ubx^lac1-GAL4 flies. (Mohit et al., 2006) Suppresses the development of ectopic scutellar macrochaetes of strain ZB1.4 (activator insertion Scer\GAL4^455.2 is combined with l(1)sc^Scer\UAS.cHa): no ectopic macrochaetes are present. (Giebel and Campos-Ortega, 1997)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	E(spl)^Scer\UAS.cGa E(spl)^UAS.cGa E(spl)m8-HLH^Scer\UAS.cGa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Giebel (Giebel and Campos-Ortega, 1997)
Secondary FlyBase IDs

References ( 6 )
Research paper	Mohit et al., 2006, Dev. Biol. 291(2): 356--367 Modulation of AP and DV signaling pathways by the homeotic gene Ultrabithorax during haltere development in Drosophila. [FBrf0190145] Kumar and Moses, 2001, Cell 104(5): 687--697 EGF receptor and Notch signaling act upstream of Eyeless/Pax6 to control eye specification. [FBrf0134490] Kumar and Moses, 2001, Development 128(14): 2689--2697 The EGF receptor and notch signaling pathways control the initiation of the morphogenetic furrow during Drosophila eye development. [FBrf0138358] Giebel, 1999, Mech. Dev. 86(1-2): 137--145 The Notch signaling pathway is required to specify muscle progenitor cells in Drosophila. [FBrf0111372] Nagel et al., 1999, Dev. Genet. 25(2): 168--179 Enhancer of split [E(spl)(D)] is a gro-independent, hypermorphic mutation in Drosophila. [FBrf0111441] Giebel and Campos-Ortega, 1997, Proc. Natl. Acad. Sci. U.S.A. 94(12): 6250--6254 Functional dissection of the Drosophila enhancer of split protein, a suppressor of neurogenesis. [FBrf0093462]

Allele Dmel\E(spl)m8-HLHScer\UAS.cGa

Allele Dmel\E(spl)m8-HLH^Scer\UAS.cGa