Dpa1/Df(2R)vg56 follicle cells show increased ploidy compared to control cells by FACS analysis.
The ovaries of Dpa1/Df(2R)vg56 females are smaller than wild-type but have the normal complement of nurse and follicle cells. Occasionally, degenerating early egg chambers (stage 8 and earlier) are seen. The predominant defects in Dpa1/Df(2R)vg56 females are a failure of nurse cells to deposit their contents into the oocyte and degenerate and an increased amount of heterochromatic DNA in the nurse cell nuclei. In homozygous Dpa1 egg chambers, unlike wild-type (where nurse cells undergo apoptosis during stages 12 to 13 and have degenerated by stage 14), degeneration of nurse cells is abnormal and occurs late if at all. Dpa1/Df(2R)vg56 mutant mothers lay few eggs. In the rare cases in which mature eggs are laid most are unfertilized, apparently due to defects in chorion structure. The few fertilized embryos arrest in early division cycles. In addition, the morphology of the polar bodies are affected. In embryos from these females meiosis is completed, but polar body chromosomes are either condensed, improperly arranged or dispersed in the embryo.
Eggs laid by hemizygous females have thin eggshells. Hemizygous ovaries show abnormal patterns of BrdU incorporation during stage 10B and later stages. The follicle cell nuclei fail to initiate amplification in most egg chambers, while in some stage 10B egg chambers genomic replication rather than amplification is seen.
Disrupt G[]-S transcription late in embryogenesis, DNA replication still occurs.
Hemizygotes exhibit rough eyes, incomplete wing vein, thin and short bristles and etched tergites. Females lay eggs with thin chorions. More females than males in progeny.
Dpa1/Dpa2 flies exhibit reduced eyes, missing and disorganised ommatidia, stunted, missing and disorganised bristles. Daily heat shocks of Dphs.PD rescued proliferation defects to normal looking eyes.
Heat induced expression of E2f1hs.PD at 25[o]C provides significant rescue of the mutant phenotype, but higher levels of expression dampen the rescue.