FlyBase Allele Report: Dmel\pio[2R-16]

General Information

Symbol

Dmel\pio^2R-16

Species

D. melanogaster

Name

FlyBase ID

FBal0063816

Feature type

allele

Associated gene

Dmel\pio

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Prout et al., 1997)

Progenitor genotype

Cytology

Description

Amino acid replacement: V159D.

(Jazwinska et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:24,595,943..24,595,943 [+]

Nucleotide change:

T24595943A

Amino acid change:

V159D | pio-PB; V159D | pio-PD; V159D | pio-PE

Reported amino acid change:

V159D

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Jazwinska et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | recessive

(Prout et al., 1997)

some die during embryonic stage | recessive

(Prout et al., 1997)

visible | somatic clone

(Prout, 1997.3.19, Prout et al., 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous pio^2R-16 embryos have a tracheal disintegration phenotype similar to Df(2R)Px2 homozygotes. All tracheal branches except the dorsal trunk and the dorsal branch of the first tracheal metamere undergo disintegration. The branches that undergo disintegration include the cerebral branch, ventral cephalic, pharyngeal branch, dorsal branch, visceral branch, transverse connective, lateral trunk anterior, lateral trunk posterior and ganglionic branch. Instead of fine tracheal tubes, in homozygous pio^2R-16 embryos these are transformed into hollow cysts. However, the dorsal trunk is not affected, indicating that pio^2R-16 embryos exhibit an absence of tubes with autocellular adherens junctions. The subsequent differentiation of the dorsal branches does not seem to be affected in pio^2R-16 homozygous embryos. Terminal branch formation or integrity is not detectably affected in pio^2R-16 homozygous embryos. In addition, the formation of fusion tubes between the bilateral dorsal branches along the dorsal midline occurs normally in pio^2R-16 homozygous embryos. prevented by a pim⁺ transgene.

(Jazwinska et al., 2003)

Homozygous clones in the wing produce large blisters that lack sharp boundaries. Wings are sometimes rumpled. Homozygous embryos show excessive contractions of the segmental muscles just before hatching. The posterior segments are compacted and the muscles appear shorter than normal.

(Prout et al., 1997)

Homozygous clones in the wing produce a blistered phenotype.

(Prout, 1997.3.19)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

pio[+]/pio^2R-16 is an enhancer of visible | recessive phenotype of mys⁸

(Prout et al., 1997)

NOT Enhancer of

Statement

Reference

pio[+]/pio^2R-16 is a non-enhancer of visible | heat sensitive phenotype of Dcr-2^UAS.cDa, Eogt^GD5084, Scer\GAL4^en.PU

(Müller et al., 2013)

NOT Suppressor of

Statement

Reference

pio[+]/pio^2R-16 is a non-suppressor of visible | heat sensitive phenotype of Dcr-2^UAS.cDa, Eogt^GD5084, Scer\GAL4^en.PU

(Müller et al., 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

pio[+]/pio^2R-16 is an enhancer of wing phenotype of mys⁸

(Prout et al., 1997)

NOT Enhancer of

Statement

Reference

pio[+]/pio^2R-16 is a non-enhancer of wing blade posterior compartment | heat sensitive phenotype of Dcr-2^UAS.cDa, Eogt^GD5084, Scer\GAL4^en.PU

(Müller et al., 2013)

NOT Suppressor of

Statement

Reference

pio[+]/pio^2R-16 is a non-suppressor of wing blade posterior compartment | heat sensitive phenotype of Dcr-2^UAS.cDa, Eogt^GD5084, Scer\GAL4^en.PU

(Müller et al., 2013)

Other

Statement

Reference

dpy^olvR, pio^2R-16 has visceral tracheal branch primordium phenotype

(Jazwinska et al., 2003)

dpy^olvR, pio^2R-16 has tracheal lateral trunk anterior branch primordium phenotype

(Jazwinska et al., 2003)

dpy^olvR, pio^2R-16 has cerebral tracheal branch phenotype

(Jazwinska et al., 2003)

dpy^olvR, pio^2R-16 has tracheal lateral trunk posterior branch primordium phenotype

(Jazwinska et al., 2003)

dpy^olvR, pio^2R-16 has adult dorsal tracheal branch phenotype

(Jazwinska et al., 2003)

dpy^olvR, pio^2R-16 has pharyngeal tracheal branch phenotype

(Jazwinska et al., 2003)

dpy^olvR, pio^2R-16 has ventral cephalic tracheal branch phenotype

(Jazwinska et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The wing blistering phenotype seen in the posterior compartment of wings in flies expressing Eogt^GD5084 under the control of Scer\GAL4^en.PU in the presence of Dcr-2^Scer\UAS.cDa is not affected if the flies are also heterozygous for pio^2R-16.

(Müller et al., 2013)

The tracheal disintegration phenotype of dp^olvR;pio^2R-16 double mutant embryos appear very similar to those observed in dp^olvR and pio^2R-16 single-mutant embryos.

(Jazwinska et al., 2003)

Shows a weak genetic interaction with mys⁸; the frequency of held-out wings but not the frequency of blisters in mys⁸ single hemizygotes is increased if they are also heterozygous for pio^2R-16. Shows no interaction with mys^b9.

(Prout et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

pio^2R-16 is rescued by Scer\GAL4^btl.PS/pio^UAS.cJa

(Jazwinska et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pio^2R-16

(Drees et al., 2023, Müller et al., 2013, Christensen et al., 2008.9.3, Christensen et al., 2008.9.3, Christensen et al., 2008.9.3)

pio^2R-1

(Müller et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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