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General Information
Symbol
Dmel\pio2R-16
Species
D. melanogaster
Name
FlyBase ID
FBal0063816
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

T24595943A

Amino acid change:

V159D | pio-PB; V159D | pio-PD; V159D | pio-PE

Reported amino acid change:

V159D

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: V159D.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous pio2R-16 embryos have a tracheal disintegration phenotype similar to Df(2R)Px2 homozygotes. All tracheal branches except the dorsal trunk and the dorsal branch of the first tracheal metamere undergo disintegration. The branches that undergo disintegration include the cerebral branch, ventral cephalic, pharyngeal branch, dorsal branch, visceral branch, transverse connective, lateral trunk anterior, lateral trunk posterior and ganglionic branch. Instead of fine tracheal tubes, in homozygous pio2R-16 embryos these are transformed into hollow cysts. However, the dorsal trunk is not affected, indicating that pio2R-16 embryos exhibit an absence of tubes with autocellular adherens junctions. The subsequent differentiation of the dorsal branches does not seem to be affected in pio2R-16 homozygous embryos. Terminal branch formation or integrity is not detectably affected in pio2R-16 homozygous embryos. In addition, the formation of fusion tubes between the bilateral dorsal branches along the dorsal midline occurs normally in pio2R-16 homozygous embryos. prevented by a pim+ transgene.

Homozygous clones in the wing produce large blisters that lack sharp boundaries. Wings are sometimes rumpled. Homozygous embryos show excessive contractions of the segmental muscles just before hatching. The posterior segments are compacted and the muscles appear shorter than normal.

Homozygous clones in the wing produce a blistered phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference

pio[+]/pio2R-16 is an enhancer of visible | recessive phenotype of mys8

NOT Enhancer of
Statement
Reference

pio[+]/pio2R-16 is a non-enhancer of visible | heat sensitive phenotype of Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU

NOT Suppressor of
Statement
Reference

pio[+]/pio2R-16 is a non-suppressor of visible | heat sensitive phenotype of Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU

Phenotype Manifest In
Enhancer of
Statement
Reference

pio[+]/pio2R-16 is an enhancer of wing phenotype of mys8

NOT Enhancer of
Statement
Reference

pio[+]/pio2R-16 is a non-enhancer of wing blade posterior compartment | heat sensitive phenotype of Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU

NOT Suppressor of
Statement
Reference

pio[+]/pio2R-16 is a non-suppressor of wing blade posterior compartment | heat sensitive phenotype of Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU

Other
Additional Comments
Genetic Interactions
Statement
Reference

The wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa is not affected if the flies are also heterozygous for pio2R-16.

The tracheal disintegration phenotype of dpolvR;pio2R-16 double mutant embryos appear very similar to those observed in dpolvR and pio2R-16 single-mutant embryos.

Shows a weak genetic interaction with mys8; the frequency of held-out wings but not the frequency of blisters in mys8 single hemizygotes is increased if they are also heterozygous for pio2R-16. Shows no interaction with mysb9.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (7)