The electroretinogram response of light-adapted ninaEΔ356;ninaE17,rdgC306 flies to oscillating light of gradually increasing frequencies is comparable to controls at both lower and higher frequencies. Dark-adapted flies show no significant changes in the onset of the frequency-locked response to oscillating light of either low (10Hz) or high (70Hz) frequency compared to wild-type flies, no defects are observed in light-adapted flies at high frequency either.
Light-induced translocation of Arr1 and ninaE endocytosis in pupal eyes is reduced in ninaEΔ356 mutants.
The reduction in electroretinogram (ERG) amplitude seen in flies exposed to constant light is greatly suppressed by ninaEΔ356. The initiation of retinal degeneration (assayed by loss of the deep pseudopupil) appears to occur earlier in ninaEΔ356 flies exposed to constant light compared to wild-type flies exposed to constant light.
When IBMX is applied to ninaEΔ356 mutant flies the macroscopic response is slowed in a way that is no different to that of wild type flies exposed to IBMX.
Transgenic adults exhibit normal retinal morphology. ERGs demonstrate the photoreceptors display normal kinetics of activation and deactivation.