Construct: Eliminates the last 18 amino acids residues that include the Ser and Thr in the cytoplasmic tail.
The electroretinogram response of light-adapted ninaEΔ356;ninaE17,rdgC306 flies to oscillating light of gradually increasing frequencies is comparable to controls at both lower and higher frequencies. Dark-adapted flies show no significant changes in the onset of the frequency-locked response to oscillating light of either low (10Hz) or high (70Hz) frequency compared to wild-type flies, no defects are observed in light-adapted flies at high frequency either.
The reduction in electroretinogram (ERG) amplitude seen in flies exposed to constant light is greatly suppressed by ninaEΔ356. The initiation of retinal degeneration (assayed by loss of the deep pseudopupil) appears to occur earlier in ninaEΔ356 flies exposed to constant light compared to wild-type flies exposed to constant light.
When IBMX is applied to ninaEΔ356 mutant flies the macroscopic response is slowed in a way that is no different to that of wild type flies exposed to IBMX.
Transgenic adults exhibit normal retinal morphology. ERGs demonstrate the photoreceptors display normal kinetics of activation and deactivation.
ninaEStoA/ninaEΔ356 is a suppressor of abnormal neuroanatomy phenotype of norpAP24
ninaEΔ356 is a suppressor of abnormal neuroanatomy phenotype of norpAP24
ninaE17/ninaEΔ356 is a suppressor of rhabdomere phenotype of norpAP24
ninaE17/ninaEΔ356 is a suppressor of photoreceptor neuron phenotype of norpAP24
ninaEΔ356 is a non-suppressor of rhabdomere R1 phenotype of norpAEE5
ninaEΔ356 is a non-suppressor of rhabdomere R2 phenotype of norpAEE5
ninaEΔ356 is a non-suppressor of rhabdomere R3 phenotype of norpAEE5
ninaEΔ356 is a non-suppressor of rhabdomere R4 phenotype of norpAEE5
ninaEΔ356 is a non-suppressor of rhabdomere R5 phenotype of norpAEE5
ninaEΔ356 is a non-suppressor of rhabdomere R6 phenotype of norpAEE5
Twenty-day old norpA36 flies expressing ninaEΔ356 exhibit the full complement of R1-R6 rhabdomeres and display no sign of retinal degeneration. Rhabdomere degeneration is delayed in norpA36 flies expressing one copy of ninaEΔ356 in a ninaE17 mutant background, as rhabdomeres retain wild-type morphology for at least 3 weeks post-eclosion. At 4 weeks, some ommatidia exhibit signs of deterioration as characterised by missing and/or reduced rhabdomeres in norpA36 flies co-expressing ninaEΔ356 (in a ninaE17 background)