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General Information
Symbol
Dmel\DrUAS.cIa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Isshiki
FlyBase ID
FBal0063863
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-msh
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

An Dr cDNA containing the entire open reading frame together with 236bp of 5' and 124bp of 3' untranslated sequences is expressed under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Overexpression of DrScer\UAS.cIa, under the regulation of Scer\GAL4ap-md544 in pharate adults results in reduced and crumpled wings, and a reduced notum. However, no hinge-like sensory organs or other structures could be discerned in the latter. Overexpression of DrScer\UAS.cIa, under the regulation of Scer\GAL4ap-md544 in the whole dorsal compartment of the disc at 17oC reduces the size of the notum territory. At 25oC, it strongly inhibits the development of this territory and individuals die at the pupal stages.

When DrScer\UAS.cIa is driven by Scer\GAL4how-24B alterations are seen both dorsal muscle and heart cell populations. The number of heart cells is dramatically reduced. The majority of pericardial cells and about 70% of cardioblasts are lacking. The majority of eve expressing DA1 muscle precursors are substituted by supernumerary DO1 muscles.

The anterior wing margin differentiates dorsal-type bristles arranged in a dorsal-like pattern on both surfaces in flies expressing DrScer\UAS.cIa under the control of Scer\GAL4C-765. The pattern of the vein L3 is symmetric, with a dorsal corrugation pattern on both surfaces. Few bristles are seen on the ventral surface of the alula, suggesting transformation to a dorsal fate. Expression of DrScer\UAS.cIa under the control of Scer\GAL4ap-md544 reduces the size of the dorsal wing pouch and induces differentiation of ectopic bristles in the wing blade. Vein differentiation is also affected.

A small fraction of flies expressing DrScer\UAS.cIa under the control of Scer\GAL4ey.PB show mild roughening of the eye. Expression of DrScer\UAS.cIa under the control of Scer\GAL4dpp.blk1 has no effect on eye development. Expression of DrScer\UAS.cIa under the control of Scer\GAL4h-1J3 results in lethality prior to the third larval instar. Expression of DrScer\UAS.cIa under the control of Scer\GAL4GMR.PF results in lethality prior to eclosion. Rare escapers exhibit severe roughening of the eye. Approximately 25% of eye discs in which DrScer\UAS.cIa is expressed under the control of Scer\GAL4hs.PB using a 1 hour heat shock (followed by a 4 hour recovery) show an arrest of the morphogenetic furrow.

The segmental border muscles are absent and the number of Kr-expressing muscles increases in embryos expressing DrScer\UAS.cIa under the control of Scer\GAL4how-24B.

Scer\GAL4how-24B-mediated expression severely alters muscle morphology, muscle may be completely missing or exhibit abnormal shape and orientation. Less severe phenotype is seen in the dorsal region, muscles are thicker than normal and occasionally contain additional muscles. Ventral internal muscles are also disorganised. Lateral transverse muscles form normally but their morphology is somewhat irregular. Ectopic Dr expression interferes with the correct specification of the progenitors/founders.

Produces severe disruptions of the axon tracts in embryos, when expressed using Scer\GAL4sca-537.4; the commissures are almost completely absent and the longitudinal connectives are broken and interrupted. Gaps are often seen along the midline in these embryos. The aCC, pCC, CQ, fpCC and RP2 neurons fail to differentiate.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

Scer\GAL4ap-md544/DrUAS.cIa is a non-suppressor of wing phenotype of apmd544/apUGO35

Additional Comments
Genetic Interactions
Statement
Reference

Expression of DrScer\UAS.cIa under the control of Scer\GAL4ap-md544 in apmd544/apUGO35 flies does not restore outgrowth of the wing, although the few margin bristles seen in the dorsal surface of these wings have dorsal identity. Co-expression of fngEP3082 and DrScer\UAS.cIa under the control of Scer\GAL4ap-md544 in apmd544/apUGO35 flies results in dorsal differentiation in bristles of the dorsal anterior wing margin.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
DrScer\UAS.cIa
DrUAS.cIa
mshScer\UAS.cIa
mshUAS.cIa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Isshiki
Secondary FlyBase IDs
    References (12)