|Name||Saccharomyces cerevisiae UAS construct a of Isshiki||FlyBase ID||FBal0063863|
|Feature type||allele||Associated gene||Dmel\Dr|
|Mutagen||in vitro construct - regulatory fusion|
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
|All updates||Click here to see a list of all updates to this record from FB2010_08 and on.|
|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
Overexpression of DrScer\UAS.cIa, under the regulation of Scer\GAL4ap-md544 in pharate adults results in reduced and crumpled wings, and a reduced notum. However, no hinge-like sensory organs or other structures could be discerned in the latter. Overexpression of DrScer\UAS.cIa, under the regulation of Scer\GAL4ap-md544 in the whole dorsal compartment of the disc at 17oC reduces the size of the notum territory. At 25oC, it strongly inhibits the development of this territory and individuals die at the pupal stages.
When DrScer\UAS.cIa is driven by Scer\GAL4how-24B alterations are seen both dorsal muscle and heart cell populations. The number of heart cells is dramatically reduced. The majority of pericardial cells and about 70% of cardioblasts are lacking. The majority of eve expressing DA1 muscle precursors are substituted by supernumerary DO1 muscles.
The anterior wing margin differentiates dorsal-type bristles arranged in a dorsal-like pattern on both surfaces in flies expressing DrScer\UAS.cIa under the control of Scer\GAL4C-765. The pattern of the vein L3 is symmetric, with a dorsal corrugation pattern on both surfaces. Few bristles are seen on the ventral surface of the alula, suggesting transformation to a dorsal fate. Expression of DrScer\UAS.cIa under the control of Scer\GAL4ap-md544 reduces the size of the dorsal wing pouch and induces differentiation of ectopic bristles in the wing blade. Vein differentiation is also affected.
A small fraction of flies expressing DrScer\UAS.cIa under the control of Scer\GAL4ey.PB show mild roughening of the eye. Expression of DrScer\UAS.cIa under the control of Scer\GAL4dpp.blk1 has no effect on eye development. Expression of DrScer\UAS.cIa under the control of Scer\GAL4h-1J3 results in lethality prior to the third larval instar. Expression of DrScer\UAS.cIa under the control of Scer\GAL4GMR.PF results in lethality prior to eclosion. Rare escapers exhibit severe roughening of the eye. Approximately 25% of eye discs in which DrScer\UAS.cIa is expressed under the control of Scer\GAL4hs.PB using a 1 hour heat shock (followed by a 4 hour recovery) show an arrest of the morphogenetic furrow.
The segmental border muscles are absent and the number of Kr-expressing muscles increases in embryos expressing Dr[Scer\UAS.cIa] under the control of Scer\GAL4[how-24B].
Scer\GAL4how-24B-mediated expression severely alters muscle morphology, muscle may be completely missing or exhibit abnormal shape and orientation. Less severe phenotype is seen in the dorsal region, muscles are thicker than normal and occasionally contain additional muscles. Ventral internal muscles are also disorganised. Lateral transverse muscles form normally but their morphology is somewhat irregular. Ectopic Dr expression interferes with the correct specification of the progenitors/founders.
Produces severe disruptions of the axon tracts in embryos, when expressed using Scer\GAL4sca-537.4; the commissures are almost completely absent and the longitudinal connectives are broken and interrupted. Gaps are often seen along the midline in these embryos. The aCC, pCC, CQ, fpCC and RP2 neurons fail to differentiate.
|Phenotype Manifest In|
|NOT Suppressor of|
Expression of DrScer\UAS.cIa under the control of Scer\GAL4ap-md544 in apmd544/apUGO35 flies does not restore outgrowth of the wing, although the few margin bristles seen in the dorsal surface of these wings have dorsal identity. Co-expression of fngEP3082 and DrScer\UAS.cIa under the control of Scer\GAL4ap-md544 in apmd544/apUGO35 flies results in dorsal differentiation in bristles of the dorsal anterior wing margin.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 4 )|
Saccharomyces cerevisiae UAS construct a of Isshiki
|Secondary FlyBase IDs|
|References ( 10 )|