Allele Dmel\Dr^Scer\UAS.cIa

General Information
Symbol	Dmel\Dr^Scer\UAS.cIa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Isshiki	FlyBase ID	FBal0063863
Feature type	allele	Associated gene	Dmel\Dr

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Isshiki et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: An Dr cDNA containing the entire open reading frame together with 236bp of 5' and 124bp of 3' untranslated sequences is expressed under the control of Scer\UAS regulatory sequences. (Isshiki et al., 1997)
Carried in construct	P{UAS-msh} (Milan et al., 2001, Villa-Cuesta and Modolell, 2005, Jagla et al., 2002, Isshiki et al., 1997, Yagi et al., 1998, Mozer, 2001, Nose et al., 1998, Von Ohlen and Moses, 2009, Müller et al., 2010, Jagla et al., 1999)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^GMR.PF (Mozer, 2001) lethal, with Scer\GAL4^h-1J3 (Mozer, 2001) lethal \| heat sensitive, with Scer\GAL4^hs.PB (Mozer, 2001) lethal \| pupal stage \| somatic clone \| heat sensitive, with Scer\GAL4^ap-md544 (Villa-Cuesta and Modolell, 2005) Minute \| somatic clone, with Scer\GAL4^ap-md544 (Villa-Cuesta and Modolell, 2005) viable, with Scer\GAL4^dpp.blk1 (Mozer, 2001) viable, with Scer\GAL4^ey.PB (Mozer, 2001) visible, with Scer\GAL4^ap-md544 (Milan et al., 2001) visible, with Scer\GAL4^C-765 (Milan et al., 2001) visible, with Scer\GAL4^ey.PB (Mozer, 2001) visible \| heat sensitive, with Scer\GAL4^hs.PB (Mozer, 2001)
Phenotype Manifest In
	A1-7 dorsal acute muscle 1, with Scer\GAL4^how-24B (Jagla et al., 2002) A1-7 dorsal oblique muscle 1 \| ectopic, with Scer\GAL4^how-24B (Jagla et al., 2002) A1-7 ventral longitudinal muscle, with Scer\GAL4^how-24B (Nose et al., 1998) A1-7 ventral oblique muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) A1-7 ventral oblique muscle 2, with Scer\GAL4^how-24B (Nose et al., 1998) A1-7 ventral oblique muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) A1-7 ventral oblique muscle 5, with Scer\GAL4^how-24B (Nose et al., 1998) A1-7 ventral oblique muscle 6, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 1 dorsal acute muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 1 dorsal oblique muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 1 dorsal oblique muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 1 dorsal oblique muscle 5, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 1 dorsal transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 1 lateral longitudinal muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 1 lateral transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 1 lateral transverse muscle 2, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 1 lateral transverse muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 1 lateral transverse muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 2 dorsal acute muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 2 dorsal oblique muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 2 dorsal oblique muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 2 dorsal oblique muscle 5, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 2 dorsal transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 2 lateral longitudinal muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 2 lateral transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 2 lateral transverse muscle 2, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 2 lateral transverse muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 2 lateral transverse muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 3 dorsal acute muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 3 dorsal oblique muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 3 dorsal oblique muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 3 dorsal oblique muscle 5, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 3 dorsal transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 3 lateral longitudinal muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 3 lateral transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 3 lateral transverse muscle 2, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 3 lateral transverse muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 3 lateral transverse muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 4 dorsal acute muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 4 dorsal oblique muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 4 dorsal oblique muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 4 dorsal oblique muscle 5, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 4 dorsal transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 4 lateral longitudinal muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 4 lateral transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 4 lateral transverse muscle 2, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 4 lateral transverse muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 4 lateral transverse muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 5 dorsal acute muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 5 dorsal oblique muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 5 dorsal oblique muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 5 dorsal oblique muscle 5, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 5 dorsal transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 5 lateral longitudinal muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 5 lateral transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 5 lateral transverse muscle 2, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 5 lateral transverse muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 5 lateral transverse muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 6 dorsal acute muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 6 dorsal oblique muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 6 dorsal oblique muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 6 dorsal oblique muscle 5, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 6 dorsal transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 6 lateral longitudinal muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 6 lateral transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 6 lateral transverse muscle 2, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 6 lateral transverse muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 6 lateral transverse muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 7 dorsal acute muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 7 dorsal oblique muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 7 dorsal oblique muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 7 dorsal oblique muscle 5, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 7 dorsal transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 7 lateral longitudinal muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 7 lateral transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 7 lateral transverse muscle 2, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 7 lateral transverse muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 7 lateral transverse muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) abdominal 8 dorsal transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) aCC neuron, with Scer\GAL4^sca-537.4 (Isshiki et al., 1997) alula \| ventral, with Scer\GAL4^C-765 (Milan et al., 2001) cardioblast, with Scer\GAL4^how-24B (Jagla et al., 2002) commissure, with Scer\GAL4^sca-537.4 (Isshiki et al., 1997) dorsal acute muscle, with Scer\GAL4^how-24B (Nose et al., 1998) dorsal oblique muscle, with Scer\GAL4^how-24B (Nose et al., 1998) dorsal transverse muscle, with Scer\GAL4^how-24B (Nose et al., 1998) embryonic pericardial cell, with Scer\GAL4^how-24B (Jagla et al., 2002) eye, with Scer\GAL4^ey.PB (Mozer, 2001) eye, with Scer\GAL4^GMR.PF (Mozer, 2001) fpCC neuron, with Scer\GAL4^sca-537.4 (Isshiki et al., 1997) heart primordium, with Scer\GAL4^how-24B (Jagla et al., 2002) hypodermal muscle of larval abdominal 9, with Scer\GAL4^how-24B (Nose et al., 1998) interommatidial bristle, with Scer\GAL4^hs.PB (Mozer, 2001) lens, with Scer\GAL4^hs.PB (Mozer, 2001) longitudinal connective, with Scer\GAL4^sca-537.4 (Isshiki et al., 1997) mesothoracic dorsal acute muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) mesothoracic dorsal oblique muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) mesothoracic dorsal oblique muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) mesothoracic lateral longitudinal muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) mesothoracic lateral transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) mesothoracic lateral transverse muscle 2, with Scer\GAL4^how-24B (Nose et al., 1998) mesothoracic lateral transverse muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) mesothoracic lateral transverse muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) mesothoracic tergum \| pharate adult stage \| somatic clone, with Scer\GAL4^ap-md544 (Villa-Cuesta and Modolell, 2005) metathoracic dorsal acute muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) metathoracic dorsal oblique muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) metathoracic dorsal oblique muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) metathoracic lateral longitudinal muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) metathoracic lateral transverse muscle 1, with Scer\GAL4^how-24B (Nose et al., 1998) metathoracic lateral transverse muscle 2, with Scer\GAL4^how-24B (Nose et al., 1998) metathoracic lateral transverse muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) metathoracic lateral transverse muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) morphogenetic furrow, with Scer\GAL4^hs.PB (Mozer, 2001) pCC neuron, with Scer\GAL4^sca-537.4 (Isshiki et al., 1997) prothoracic dorsal oblique muscle 3, with Scer\GAL4^how-24B (Nose et al., 1998) prothoracic dorsal oblique muscle 4, with Scer\GAL4^how-24B (Nose et al., 1998) RP2 motor neuron, with Scer\GAL4^sca-537.4 (Isshiki et al., 1997) segment border muscle, with Scer\GAL4^how-24B (Jagla et al., 1999) U neuron, with Scer\GAL4^sca-537.4 (Isshiki et al., 1997) ventral midline, with Scer\GAL4^sca-537.4 (Isshiki et al., 1997) wing \| dorsal, with Scer\GAL4^ap-md544 (Milan et al., 2001) wing \| pharate adult stage \| somatic clone, with Scer\GAL4^ap-md544 (Villa-Cuesta and Modolell, 2005) wing margin \| anterior \| ventral, with Scer\GAL4^C-765 (Milan et al., 2001) wing sensillum \| ectopic, with Scer\GAL4^ap-md544 (Milan et al., 2001) wing vein, with Scer\GAL4^ap-md544 (Milan et al., 2001) wing vein L3 \| ventral, with Scer\GAL4^C-765 (Milan et al., 2001)
Detailed Description
	Statement Reference Overexpression of Dr^Scer\UAS.cIa, under the regulation of Scer\GAL4^ap-md544 in pharate adults results in reduced and crumpled wings, and a reduced notum. However, no hinge-like sensory organs or other structures could be discerned in the latter. Overexpression of Dr^Scer\UAS.cIa, under the regulation of Scer\GAL4^ap-md544 in the whole dorsal compartment of the disc at 17^oC reduces the size of the notum territory. At 25^oC, it strongly inhibits the development of this territory and individuals die at the pupal stages. (Villa-Cuesta and Modolell, 2005) When Dr^Scer\UAS.cIa is driven by Scer\GAL4^how-24B alterations are seen both dorsal muscle and heart cell populations. The number of heart cells is dramatically reduced. The majority of pericardial cells and about 70% of cardioblasts are lacking. The majority of eve expressing DA1 muscle precursors are substituted by supernumerary DO1 muscles. (Jagla et al., 2002) The anterior wing margin differentiates dorsal-type bristles arranged in a dorsal-like pattern on both surfaces in flies expressing Dr^Scer\UAS.cIa under the control of Scer\GAL4^C-765. The pattern of the vein L3 is symmetric, with a dorsal corrugation pattern on both surfaces. Few bristles are seen on the ventral surface of the alula, suggesting transformation to a dorsal fate. Expression of Dr^Scer\UAS.cIa under the control of Scer\GAL4^ap-md544 reduces the size of the dorsal wing pouch and induces differentiation of ectopic bristles in the wing blade. Vein differentiation is also affected. (Milan et al., 2001) A small fraction of flies expressing Dr^Scer\UAS.cIa under the control of Scer\GAL4^ey.PB show mild roughening of the eye. Expression of Dr^Scer\UAS.cIa under the control of Scer\GAL4^dpp.blk1 has no effect on eye development. Expression of Dr^Scer\UAS.cIa under the control of Scer\GAL4^h-1J3 results in lethality prior to the third larval instar. Expression of Dr^Scer\UAS.cIa under the control of Scer\GAL4^GMR.PF results in lethality prior to eclosion. Rare escapers exhibit severe roughening of the eye. Approximately 25% of eye discs in which Dr^Scer\UAS.cIa is expressed under the control of Scer\GAL4^hs.PB using a 1 hour heat shock (followed by a 4 hour recovery) show an arrest of the morphogenetic furrow. (Mozer, 2001) The segmental border muscles are absent and the number of Kr-expressing muscles increases in embryos expressing Dr[Scer\UAS.cIa] under the control of Scer\GAL4[how-24B]. (Jagla et al., 1999) Scer\GAL4^how-24B-mediated expression severely alters muscle morphology, muscle may be completely missing or exhibit abnormal shape and orientation. Less severe phenotype is seen in the dorsal region, muscles are thicker than normal and occasionally contain additional muscles. Ventral internal muscles are also disorganised. Lateral transverse muscles form normally but their morphology is somewhat irregular. Ectopic Dr expression interferes with the correct specification of the progenitors/founders. (Nose et al., 1998) Produces severe disruptions of the axon tracts in embryos, when expressed using Scer\GAL4^sca-537.4; the commissures are almost completely absent and the longitudinal connectives are broken and interrupted. Gaps are often seen along the midline in these embryos. The aCC, pCC, CQ, fpCC and RP2 neurons fail to differentiate. (Isshiki et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Scer\GAL4^ap-md544/Dr^Scer\UAS.cIa is a suppressor \| partially of visible phenotype of ap^md544/ap^UGO35 (Milan et al., 2001)
Phenotype Manifest In
Suppressor of
Statement Reference Scer\GAL4^ap-md544/Dr^Scer\UAS.cIa is a suppressor \| partially of wing margin phenotype of ap^md544/ap^UGO35 (Milan et al., 2001)
NOT Suppressor of
Statement Reference Scer\GAL4^ap-md544/Dr^Scer\UAS.cIa is a non-suppressor of wing phenotype of ap^md544/ap^UGO35 (Milan et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference Expression of Dr^Scer\UAS.cIa under the control of Scer\GAL4^ap-md544 in ap^md544/ap^UGO35 flies does not restore outgrowth of the wing, although the few margin bristles seen in the dorsal surface of these wings have dorsal identity. Co-expression of fng^EP3082 and Dr^Scer\UAS.cIa under the control of Scer\GAL4^ap-md544 in ap^md544/ap^UGO35 flies results in dorsal differentiation in bristles of the dorsal anterior wing margin. (Milan et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^how-24B, Scer\GAL4^how-24B, Dr^Scer\UAS.cIa rescues Dr^Δ68 (Nose et al., 1998)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Dr^Scer\UAS.cIa msh^Scer\UAS.cIa (Nose et al., 1998, ) msh^UAS.cIa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Isshiki (Isshiki et al., 1997)
Secondary FlyBase IDs

References ( 10 )
Research paper	Müller et al., 2010, PLoS ONE 5(12): e14323 Regulation and Functions of the lms Homeobox Gene during Development of Embryonic Lateral Transverse Muscles and Direct Flight Muscles in Drosophila. [FBrf0212632] Von Ohlen and Moses, 2009, Mech. Dev. 126(7): 552--562 Identification of Ind transcription activation and repression domains required for dorsoventral patterning of the CNS. [FBrf0208241] Villa-Cuesta and Modolell, 2005, Development 132(18): 4087--4096 Mutual repression between msh and Iro-C is an essential component of the boundary between body wall and wing in Drosophila. [FBrf0187488] Jagla et al., 2002, Development 129(4): 1037--1047 Cross-repressive interactions of identity genes are essential for proper specification of cardiac and muscular fates in Drosophila. [FBrf0144816] Milan et al., 2001, Development 128(17): 3263--3268 msh specifies dorsal cell fate in the Drosophila wing. [FBrf0138371] Mozer, 2001, Dev. Biol. 233(2): 380--393 Dominant Drop mutants are gain-of-function alleles of the muscle segment homeobox gene (msh) whose overexpression leads to the arrest of eye development. [FBrf0136848] Jagla et al., 1999, Int. J. Dev. Biol. 43(6): 571--573 Plasticity within the lateral somatic mesoderm of Drosophila embryos. [FBrf0123062] Nose et al., 1998, Development 125(2): 215--223 Regional specification of muscle progenitors in Drosophila: the role of the msh homeobox gene. [FBrf0100612] Yagi et al., 1998, Development 125(18): 3625--3633 Interaction between Drosophila EGF receptor and vnd determines three dorsoventral domains of the neuroectoderm. [FBrf0104795] Isshiki et al., 1997, Development 124(16): 3099--3109 The role of the msh homeobox gene during Drosophila neurogenesis: implication for the dorsoventral specification of the neuroectoderm. [FBrf0098274]

Allele Dmel\DrScer\UAS.cIa

Allele Dmel\Dr^Scer\UAS.cIa