Open Close
General Information
Symbol
Dmel\Tork17004
Species
D. melanogaster
Name
FlyBase ID
FBal0064028
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dTORP1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The P{lacW} insertion is located 211bp upstream of the putative translation start of Tor, and within the 5' UTR.

The P{lacW} insertion is located 24bp downstream of the transcription start site.

Insertion components
P{lacW}Tork17004
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Tork17004/Tork17004 larvae show a severe growth defect and are smaller in size than control larvae.

Tork17004/+ larvae develop normally.

Tork17004/+ flies have eyes similar to wild-type.

The body mass of third instar larvae is reduced for homozygous Tork17004 mutants.

Homozygous Tork17004 mutant third instar larvae show increased uptake and transport of ingested fluorescently labeled fatty acids from the midgut lumen to the fat body compared to wild-type controls.

Mutant flies fed a high-fat diet do not show an increase in triglyceride levels, in contrast to wild-type controls fed this diet.

Mutant flies have impaired geotaxic ability when fed a normal food diet, and this phenotype is exacerbated on a high-fat diet.

Mutant flies show considerable protection against deterioration of heart function compared to controls when placed on a high-fat diet. The mutant flies have unchanged heartbeat and contractility parameters on a normal food diet and on a high-fat diet, which are comparable to the values seen in wild-type flies on a normal food diet. The incidences of conduction blocks, dysfunctional ostia and non-contractile myocardial regions are as low in the mutant flies under normal food or high-fat diet conditions as in wild-type flies on a normal food diet. Myocardial structure remains intact in the mutant flies under a high-fat diet.

Tork17004 homozygous animals show growth arrest in the second/early third instar larval stages, and markedly delayed growth.

Tork17004 homozygous animals show lipid vesicle aggregation in the fat body of second/early third instar larvae.

Cell and nuclear size of the salivary gland cells are markedly reduced in Tork17004 larvae.

Tor2L1/Tork17004 mutants have wing discs that are approximately wild-type in size. The size of the nuclei in the endoreplicating salivary glands are smaller than wild-type.

Homozygotes hatch at normal rates, but grow more slowly than normal and eventually arrest during larval development, reaching only 40% the mass of wild-type controls. The mutants remain viable and active during an extended larval period of about 30 days and eventually die without pupating.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
Statement
Reference

Tor[+]/Tork17004 is an enhancer of some die during P-stage | heat sensitive phenotype of LpindsRNA.hs

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

Tor[+]/Tork17004 is a non-suppressor of lethal | larval stage phenotype of gig193/gig109

Other
Statement
Reference

LpindsRNA.hs, Tor[+]/Tork17004 has visible | larval stage | heat sensitive phenotype

LpindsRNA.hs, Tor[+]/Tork17004 has size defective | larval stage | heat sensitive phenotype

Phenotype Manifest In
Suppressed by
Statement
Reference

Tork17004 has nucleus phenotype, suppressible | partially by Atg1[+]/Atg1Δ3D

Tork17004 has fat body | second instar larval stage phenotype, suppressible by Atg1[+]/Atg1EP3348

Tork17004 has vesicle phenotype, suppressible by Atg1[+]/Atg1EP3348

Tork17004 has nucleus phenotype, suppressible | partially by Atg1[+]/Atg1EP3348

Tork17004 has fat body | second instar larval stage phenotype, suppressible by Atg1[+]/Atg1Δ3D

Tork17004 has vesicle phenotype, suppressible by Atg1[+]/Atg1Δ3D

Enhancer of
Statement
Reference

Tor[+]/Tork17004 is an enhancer of eye phenotype of EBV\BZLF1GMR.PA

NOT Enhancer of
Statement
Reference

Tor[+]/Tork17004 is a non-enhancer of lipid droplet | larval stage | heat sensitive phenotype of LpindsRNA.hs

Suppressor of
Statement
Reference

Tor[+]/Tork17004 is a suppressor | partially of eye phenotype of EBV\BRLF1GMR.PA

Tor[+]/Tork17004 is a suppressor | partially of ommatidium phenotype of EBV\BRLF1GMR.PA

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Tork17004/+ in larvae expressing LpindsRNA.hs (induced by heat shock) leads to a reduction in fat body mass and a transparent appearance of larvae; an increase in pupal lethality as compared to larvae expressing LpindsRNA.hs in a wild type background; and the fat bodies from these larvae are smaller; but lipid droplets in the fat bodies of these larvae exhibit a similar size reduction as those in larvae expressing LpindsRNA.hs in a wild type background.

Tork17004 partially suppresses the rough eye phenotype seen when Dcp-1Scer\UAS.cKa (insertion line P{UAS-Dcp-1.K}19-2) is expressed under the control of Scer\GAL4GMR.PF.

Tork17004 homozygous animals in a heterozygous Atg1EP3348 or Atg1Δ3D genetic background grow faster, and extend their developmental stage to mid/late third instar larval stage. Double homozygous animals survive to mid-late third instar larval stage.

Lipid vesicle aggregation in the fat body of Tork17004 homozygous second/early third instar larvae is suppressed in a heterozygous Atg1EP3348 or Atg1Δ3D genetic background.

Tork17004 mutant larvae heterozygous for Atg1EP3348 or Atg1Δ3D show a partially rescued cell and nuclear salivary gland size.

Xenogenetic Interactions
Statement
Reference

Tork17004/+ partially suppresses the eye-phenotype associated with EBV\BRLF1GMR.PA-expression.

Tork17004/+ partially enhances the eye-phenotype associated with EBV\BRLF1GMR.PA-expression.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Complements: Vha68-2k02508. Complements: l(2)k07015k07015. Complements: l(2)k07332k07332. Complements: Vha68-2k07404.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
References (17)