Tork17004/Tork17004 larvae show a severe growth defect and are smaller in size than control larvae.
Tork17004/+ larvae develop normally.
Tork17004/+ flies have eyes similar to wild-type.
The body mass of third instar larvae is reduced for homozygous Tork17004 mutants.
Homozygous Tork17004 mutant third instar larvae show increased uptake and transport of ingested fluorescently labeled fatty acids from the midgut lumen to the fat body compared to wild-type controls.
Mutant flies fed a high-fat diet do not show an increase in triglyceride levels, in contrast to wild-type controls fed this diet.
Mutant flies have impaired geotaxic ability when fed a normal food diet, and this phenotype is exacerbated on a high-fat diet.
Mutant flies show considerable protection against deterioration of heart function compared to controls when placed on a high-fat diet. The mutant flies have unchanged heartbeat and contractility parameters on a normal food diet and on a high-fat diet, which are comparable to the values seen in wild-type flies on a normal food diet. The incidences of conduction blocks, dysfunctional ostia and non-contractile myocardial regions are as low in the mutant flies under normal food or high-fat diet conditions as in wild-type flies on a normal food diet. Myocardial structure remains intact in the mutant flies under a high-fat diet.
Tork17004 homozygous animals show growth arrest in the second/early third instar larval stages, and markedly delayed growth.
Tork17004 homozygous animals show lipid vesicle aggregation in the fat body of second/early third instar larvae.
Cell and nuclear size of the salivary gland cells are markedly reduced in Tork17004 larvae.
Tor2L1/Tork17004 mutants have wing discs that are approximately wild-type in size. The size of the nuclei in the endoreplicating salivary glands are smaller than wild-type.
Homozygotes hatch at normal rates, but grow more slowly than normal and eventually arrest during larval development, reaching only 40% the mass of wild-type controls. The mutants remain viable and active during an extended larval period of about 30 days and eventually die without pupating.