Homozygous mutant RpI135k16513 clones can be rescued by genetically reducing the growth of surrounding cells by making them heterozygous for a dominant Minute RpS131 mutant. In this case, RpI135k16513 mutant clones are recovered in all RpS131 heterozygous discs that are examined at 96hrs after clone induction, a time-point at which all clones are normally eliminated in a wild-type background. However, these clones colonise a significantly smaller area of RpS131/+ discs compared with wild-type clones (28.8% compared to 67.8% of the total disc area), indicating that they are still growth impaired. Compared to surrounding heterozygote RpS131 or wild-type cells, RpI135k16513 mutant clone cells exhibit no significant change in either cell size or cell-cycle phasing.
Homozygous mutant RpI135k16513 clone survival can be rescued by co-expression of the apoptosis inhibitor, BacA\p35Scer\UAS.cHa under the control of Scer\GAL4αTub84B.PL. However, the clones are still growth defective, indicating that the reduced growth and viability of mutant clones is not simply due to increased cell death.