Open Close
General Information
Symbol
Dmel\RpI135k16513
Species
D. melanogaster
Name
FlyBase ID
FBal0064049
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
l(2)k16513
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
P{lacW}RpI135k16513
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Homozygous RpI135k16513 mutant larvae hatch and are viable. However, they remain as growth-arrested L1 larvae and survive for up to 8 days. RpI135k16513 homozygous cells in somatic clones in the larval fat body are small and have decreased DNA content compared to surrounding endoreplicated cells. RpI135k16513 homozygous somatic clones in the wing disc are about 50% smaller than wild-type twin-spots, but the cells in these clones are of normal size, and show no shift in cell-cycle phasing compared to wild-type siblings. The viability of these clones decreases with time after induction.
Viable in combination with RpI135k16513.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
RpI135k16513/+ partially suppresses the semi-lethality caused by expression of Ras85DV12.Scer\UAS under the control of Scer\GAL4ey.PU at 21-22[o]C.
Homozygous mutant RpI135k16513 clones can be rescued by genetically reducing the growth of surrounding cells by making them heterozygous for a dominant Minute RpS131 mutant. In this case, RpI135k16513 mutant clones are recovered in all RpS131 heterozygous discs that are examined at 96hrs after clone induction, a time-point at which all clones are normally eliminated in a wild-type background. However, these clones colonise a significantly smaller area of RpS131/+ discs compared with wild-type clones (28.8% compared to 67.8% of the total disc area), indicating that they are still growth impaired. Compared to surrounding heterozygote RpS131 or wild-type cells, RpI135k16513 mutant clone cells exhibit no significant change in either cell size or cell-cycle phasing.
Xenogenetic Interactions
Statement
Reference
Homozygous mutant RpI135k16513 clone survival can be rescued by co-expression of the apoptosis inhibitor, BacA\p35Scer\UAS.cHa under the control of Scer\GAL4αTub84B.PL. However, the clones are still growth defective, indicating that the reduced growth and viability of mutant clones is not simply due to increased cell death.
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments
Complements: ex01270. Complements: lwr01519a. Complements: ex02278. Complements: ds05142. Complements: α-Adaptin06694. Complements: exk01115. Complements: ebik16213. Complements: kisk16510.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
RpI135K16513
RpI135k16513
Rpl135k16513
l(2)k16513k16513
Name Synonyms
Secondary FlyBase IDs
    References (7)