FB2020_06, released Dec 22, 2020

Allele: Dmel\RpI135k16513

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General Information
Symbol
Dmel\RpI135k16513
Species
D. melanogaster
Name
FlyBase ID
FBal0064049
Feature type
allele
Associated gene
Dmel\RpI135
Associated Insertion(s)
P{lacW}RpI135k16513
Carried in Construct
Also Known As
l(2)k16513
Genomic Maps
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(BDGP Project Members, 1994-1999)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
transposable element insertion site
2L:404,312..404,312 [+]
(Deal and Cook, 2005.3.10, Deal and Cook, 2001.4.24, Spradling et al., 1999, Campbell and Tomlinson, 1998, BDGP Project Members, 1994-1999)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Cytology
      Nature of the lesion
      Statement
      Reference
      Insertion components
      P{lacW}RpI135k16513
      Product class / Tool use(s)
      Encoded product / tool
      lacZ
      Tagged with
      Tag:NLS(P)
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous RpI135k16513 mutant larvae hatch and are viable. However, they remain as growth-arrested L1 larvae and survive for up to 8 days. RpI135k16513 homozygous cells in somatic clones in the larval fat body are small and have decreased DNA content compared to surrounding endoreplicated cells. RpI135k16513 homozygous somatic clones in the wing disc are about 50% smaller than wild-type twin-spots, but the cells in these clones are of normal size, and show no shift in cell-cycle phasing compared to wild-type siblings. The viability of these clones decreases with time after induction.

      (Grewal et al., 2005)

      Viable in combination with RpI135k16513.

      (Campbell and Tomlinson, 1998)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      RpI135k16513/+ partially suppresses the semi-lethality caused by expression of Ras85DV12.Scer\UAS under the control of Scer\GAL4ey.PU at 21-22[o]C.

      (Gaur et al., 2013)

      Homozygous mutant RpI135k16513 clones can be rescued by genetically reducing the growth of surrounding cells by making them heterozygous for a dominant Minute RpS131 mutant. In this case, RpI135k16513 mutant clones are recovered in all RpS131 heterozygous discs that are examined at 96hrs after clone induction, a time-point at which all clones are normally eliminated in a wild-type background. However, these clones colonise a significantly smaller area of RpS131/+ discs compared with wild-type clones (28.8% compared to 67.8% of the total disc area), indicating that they are still growth impaired. Compared to surrounding heterozygote RpS131 or wild-type cells, RpI135k16513 mutant clone cells exhibit no significant change in either cell size or cell-cycle phasing.

      (Grewal et al., 2005)
      Xenogenetic Interactions
      Statement
      Reference

      Homozygous mutant RpI135k16513 clone survival can be rescued by co-expression of the apoptosis inhibitor, BacA\p35Scer\UAS.cHa under the control of Scer\GAL4αTub84B.PL. However, the clones are still growth defective, indicating that the reduced growth and viability of mutant clones is not simply due to increased cell death.

      (Grewal et al., 2005)
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      I. Kiss.

      (BDGP Project Members, 1994-1999)
      Comments
      Comments

      Complements: ex01270. Complements: lwr01519a. Complements: ex02278. Complements: ds05142. Complements: α-Adaptin06694. Complements: exk01115. Complements: ebik16213. Complements: kisk16510.

      (BDGP Project Members, 1994-1999)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      RpI135K16513
      (Grewal et al., 2005)
      RpI135k16513
      Rpl135k16513
      (Gaur et al., 2013)
      l(2)k16513
      (Spradling et al., 1999, Campbell and Tomlinson, 1998)
      l(2)k16513k16513
      Name Synonyms
      Secondary FlyBase IDs
        References (7)