Homozygous RpI135k16513 mutant larvae hatch and are viable. However, they remain as growth-arrested L1 larvae and survive for up to 8 days. RpI135k16513 homozygous cells in somatic clones in the larval fat body are small and have decreased DNA content compared to surrounding endoreplicated cells. RpI135k16513 homozygous somatic clones in the wing disc are about 50% smaller than wild-type twin-spots, but the cells in these clones are of normal size, and show no shift in cell-cycle phasing compared to wild-type siblings. The viability of these clones decreases with time after induction.
Viable in combination with RpI135k16513.
RpI135k16513 has increased cell death | somatic clone | larval stage phenotype, suppressible | partially by RpS131/RpS13[+]
RpI135k16513 has increased cell death | somatic clone | larval stage phenotype, suppressible by Scer\GAL4αTub84B.PL/BacA\p35UAS.cHa
RpI135k16513 has decreased cell number | somatic clone | larval stage phenotype, non-suppressible by RpS131/RpS13[+]
RpI135k16513 has decreased cell number | somatic clone | larval stage phenotype, non-suppressible by Scer\GAL4αTub84B.PL/BacA\p35UAS.cHa
RpI135[+]/RpI135k16513 is a suppressor | partially of partially lethal - majority die phenotype of Ras85DV12.UAS, Scer\GAL4ey.PU
RpI135k16513 is a suppressor of increased cell size | somatic clone phenotype of Scer\GAL4αTub84B.PL, MycUAS.cZa
RpI135k16513 is a suppressor of increased occurrence of cell division | somatic clone | larval stage phenotype of MycUAS.cZa, Scer\GAL4αTub84B.PL
RpI135k16513/+ partially suppresses the semi-lethality caused by expression of Ras85DV12.Scer\UAS under the control of Scer\GAL4ey.PU at 21-22[o]C.
Homozygous mutant RpI135k16513 clones can be rescued by genetically reducing the growth of surrounding cells by making them heterozygous for a dominant Minute RpS131 mutant. In this case, RpI135k16513 mutant clones are recovered in all RpS131 heterozygous discs that are examined at 96hrs after clone induction, a time-point at which all clones are normally eliminated in a wild-type background. However, these clones colonise a significantly smaller area of RpS131/+ discs compared with wild-type clones (28.8% compared to 67.8% of the total disc area), indicating that they are still growth impaired. Compared to surrounding heterozygote RpS131 or wild-type cells, RpI135k16513 mutant clone cells exhibit no significant change in either cell size or cell-cycle phasing.
Homozygous mutant RpI135k16513 clone survival can be rescued by co-expression of the apoptosis inhibitor, BacA\p35Scer\UAS.cHa under the control of Scer\GAL4αTub84B.PL. However, the clones are still growth defective, indicating that the reduced growth and viability of mutant clones is not simply due to increased cell death.
I. Kiss.
Complements: ex01270. Complements: lwr01519a. Complements: ex02278. Complements: ds05142. Complements: α-Adaptin06694. Complements: exk01115. Complements: ebik16213. Complements: kisk16510.