FlyBase Allele Report: Dmel\geminin[k14019]

General Information

Symbol

Dmel\geminin^k14019

Species

D. melanogaster

Name

FlyBase ID

FBal0064112

Feature type

allele

Associated gene

Dmel\geminin

Associated Insertion(s)

P{lacW}geminin^k14019

Carried in Construct

Also Known As

l(2)k14019

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Roch et al., 1998, BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}geminin^k14019

Cytology

Description

P{lacW} insertion at +104bp relative to the transcription start site.

(Quinn et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:6,656,242..6,656,242 [+]

(Herr et al., 2010, Quinn et al., 2001, Spradling et al., 1999, Roch et al., 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased occurrence of cell division | embryonic stage (with geminin^k09107)

(Quinn et al., 2001)

lethal (with Df(2R)ST1)

(Quinn et al., 2001)

lethal | recessive

(Quinn et al., 2001, Spradling et al., 1999, Roch et al., 1998)

Phenotype Manifest In

follicle cell (with geminin^k09107)

(Quinn et al., 2001)

imaginal disc

(Roch et al., 1998)

Detailed Description

Statement

Reference

Lethal phase is during embryogenesis/larval stages. 40% of geminin^k14019/Df(2R)ST1 animals die before hatching and the rest die during larval development (third instar larvae are occasionally seen). 100% of stage 12 geminin^k14019/geminin^k09107 ovaries show strong BrdU labeling in 4 amplification foci in all follicle cells (wild-type ovaries at this stage mostly have only one focus of DNA amplification per cell). By stage 14, all follicle cells of wild-type ovaries have ceased DNA amplification, but many follicle cells from 50% of mutant ovaries still continue DNA amplification.

(Quinn et al., 2001)

Lethality occurs during third instar larval or pupal stages. Mutants exhibit visible disc abnormalities: very small or no discs. Clones cannot be induced in wild type discs. In tergites, clones with normal size and but lower than expected frequency are recovered. Clones cannot be recovered in the wing disc.

(Roch et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

geminin[+]/geminin^k14019, Scer\GAL4^bbg-C96 is an enhancer of visible phenotype of Raf^UAS.F179, Scer\GAL4^bbg-C96

(Herr et al., 2010)

Phenotype Manifest In

Enhancer of

Statement

Reference

geminin[+]/geminin^k14019, Scer\GAL4^bbg-C96 is an enhancer of wing margin phenotype of Raf^UAS.F179, Scer\GAL4^bbg-C96

(Herr et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

geminin^k14019/+ causes a significant worsening of the notched wing phenotype resulting from the expression of phl^{Scer\UAS.F179} via Scer\GAL4^bbg-C96.

(Herr et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

geminin^k03202b

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Kyoto

108863

y¹ w^67c23; P{lacW}geminin^k14019/CyO

102909

y¹ w^67c23 ; P{lacW}geminin^k14019 / CyO

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Complements: Adf1⁰¹³⁴⁹. Complements: Adf1⁰⁴⁰⁶⁵. Complements: l(2)04535b^04535b. Complements: l(2)10521¹⁰⁵²¹. Complements: Adf1^k00620. Complements: l(2)04535b^k01601. Complements: l(2)k09107^k09107. Complements: l(2)04535b^k09819. Complements: Adf1^k09919. Complements: l(2)04535b^k14807.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

140/19

(Roch et al., 1998)

geminin^k14019

l(2)k14019

(Quinn et al., 2001, Spradling et al., 1999)

l(2)k14019^k14019

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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