FlyBase Allele Report: Dmel\Nle[k13714]

General Information

Symbol

Dmel\Nle^k13714

Species

D. melanogaster

Name

FlyBase ID

FBal0064122

Feature type

allele

Associated gene

Dmel\Nle

Associated Insertion(s)

P{lacW}Nle^k13714

Carried in Construct

Also Known As

l(2)k13714

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Royet et al., 1998)

Mutagen

P-element activity

(Spradling et al., 1999, FlyBase, 1996-, BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Nle^k13714

Cytology

Description

P{lacW} is inserted 310bp 5' to the start of the Nle open reading frame.

(Royet et al., 1998)

The P{lacW}Nle^k13714 insertion is identified as an allele of Nle on genetic grounds (see FBrf0105918) as well as an allele of CG2807 on molecular grounds (it is within the CG2807 transcription unit).

(FlyBase, 1996-)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:568,371..568,371 [+]

(Deal and Cook, 2005.3.10, Spradling et al., 1999, FlyBase, 1996-, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Spradling et al., 1999)

male sterile

(Royet et al., 1998)

viable

(Royet et al., 1998)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygotes are viable, morphologically normal and male sterile.

(Royet et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Nle[+]/Nle^k13714 is an enhancer of orbital bristle phenotype of N^Ax-1

(Royet et al., 1998)

Nle[+]/Nle^k13714 is an enhancer of dorsocentral bristle phenotype of N^Ax-1

(Royet et al., 1998)

Nle[+]/Nle^k13714 is an enhancer of wing vein phenotype of N^Ax-1

(Royet et al., 1998)

Suppressor of

Statement

Reference

Nle[+]/Nle^k13714 is a suppressor of wing phenotype of N^nd-1

(Royet et al., 1998)

Nle[+]/Nle^k13714 is a suppressor of wing vein phenotype of N^nd-1

(Royet et al., 1998)

Nle[+]/Nle^k13714 is a suppressor of wing phenotype of N^nd-2

(Royet et al., 1998)

Nle[+]/Nle^k13714 is a suppressor of wing vein phenotype of N^nd-2

(Royet et al., 1998)

Nle[+]/Nle^k13714 is a suppressor of wing phenotype of N^nd-3

(Royet et al., 1998)

Nle[+]/Nle^k13714 is a suppressor of wing vein phenotype of N^nd-3

(Royet et al., 1998)

Nle^k13714 is a suppressor of wing phenotype of dx¹

(Royet et al., 1998)

Nle^k13714 is a suppressor of wing vein phenotype of dx¹

(Royet et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Dominantly suppresses wing margin and vein phenotypes of N^nd-1 and N^nd-2 though wing veins are interrupted due to overactivation of N signalling pathway. Dominantly suppresses wing margin and vein phenotypes of N^nd-3, and dx¹. Enhances the bristle and wing vein phenotypes of gain of function N^Ax-1. Reveals sub-threshold effect of dx^hs.PB, in effect on wing veins.