FlyBase Allele Report: Dmel\Sps1[k11320]

(Morey et al., 2003, Morey et al., 2001, Alsina et al., 1999, Spradling et al., 1999, Alsina et al., 1998, Roch et al., 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval optic lobe

(Alsina et al., 1999)

imaginal disc

(Alsina et al., 1999, Alsina et al., 1998, Roch et al., 1998)

larval brain

(Alsina et al., 1999, Alsina et al., 1998)

larval ventral nerve cord | larval stage

(Alsina et al., 1999)

wing

(Roch et al., 1998)

wing vein

(Alsina et al., 1998, Roch et al., 1998)

Detailed Description

Statement

Reference

No tracheal defects are apparent in SelD^k11320 mutants.

(Swanson et al., 2009)

Heterozygous flies develop into normal-appearing, viable adults which can mate and give progeny. Heterozygous males do not show a significantly different mean life span than controls. Heterozygous male adults show hypersensitivity to paraquat and increased sensitivity to hydrogen peroxide compared to controls.

(Morey et al., 2003)

Homozygotes die during the third larval instar stage. Mutant brains do not have optic lobes. The pattern of BrdU incorporation indicates that proliferation is significantly reduced in the brain hemispheres and ventral ganglion compared to wild type. The percentage of cells incorporating BrdU in homozygous imaginal discs (less than 10%) is reduced compared to wild-type (34%).

(Alsina et al., 1999)

Homozygous larvae die at around days 8 and 9 AEL without entering pupation. Dissected mutants exhibit an aberrant imaginal disc morphology characterised by a reduction in mass of discs and disorganisation of disc cells where no folding or patterning can be detected. Apoptotic cells are observed in these small and abnormal mutant discs. Larval brain hemispheres are smaller than wild type. Mutants transformed with the full-length cDNA UAS construct expressed from Scer\GAL4^69B show complete reversion of lethality and disc phenotype. Mitotic clones cause reduced cell viability, smaller cell and clone size and clones in the wing stop wing vein differentiation. Non-autonomous effects, such as abnormal differentiation of wild type cells surrounding the clones, are also seen: change in orientation of surrounding wild type trichomes and extra veins.

(Alsina et al., 1998)

Mutants exhibit visible disc abnormalities: very small or no discs. Clones induced in wild type discs are abnormal: clone are small and densely packed with contours that appear smooth defining sharp borders. Clones on the wing can affect wing veins and have non-autonomous effects on neighbouring cells.

(Roch et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Sps1^k11320 is a non-enhancer of visible phenotype of rl^Sem

(Morey et al., 2001)

Suppressor of

Statement

Reference

Sps1^k11320 is a suppressor of visible | dominant phenotype of Raf::tor^12D.sev

(Morey et al., 2001)

Sps1^k11320 is a suppressor of visible phenotype of sev^S11.Tag:MYC

(Morey et al., 2001)

NOT Suppressor of

Statement

Reference

Sps1^k11320 is a non-suppressor of visible phenotype of Ras64B^V12.sev

(Morey et al., 2001)

Sps1^k11320 is a non-suppressor of visible phenotype of rl^Sem

(Morey et al., 2001)

Sps1^k11320 is a non-suppressor of visible phenotype of Rho1^GMR.PH

(Morey et al., 2001)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Sps1^k11320 is a non-enhancer of eye phenotype of rl^Sem

(Morey et al., 2001)

Sps1^k11320 is a non-enhancer of ommatidium phenotype of wg^hs.2sev

(Morey et al., 2001)

Sps1^k11320 is a non-enhancer of photoreceptor neuron phenotype of rl^Sem

(Morey et al., 2001)

Sps1^k11320 is a non-enhancer of wing vein L4 phenotype of N^Ax-1

(Morey et al., 2001)

Sps1^k11320 is a non-enhancer of wing vein L5 phenotype of N^Ax-1

(Morey et al., 2001)

Sps1^k11320 is a non-enhancer of eye phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^GMR.PF

(Morey et al., 2001)

Sps1^k11320 is a non-enhancer of wing vein | ectopic phenotype of Scer\GAL4⁶⁰⁴, tkv^{Q253D.UAS.cLa}

(Morey et al., 2001)

Sps1^k11320 is a non-enhancer of wing phenotype of Scer\GAL4⁶⁰⁴, tkv^{Q253D.UAS.cLa}

(Morey et al., 2001)

Sps1^k11320 is a non-enhancer of eye phenotype of wg^hs.2sev

(Morey et al., 2001)

Sps1^k11320 is a non-enhancer of interommatidial bristle phenotype of wg^hs.2sev

(Morey et al., 2001)

Suppressor of

Statement

Reference

Sps1^k11320 is a suppressor of wing vein | ectopic phenotype of Egfr^E3

(Morey et al., 2001)

Sps1^k11320 is a suppressor of eye phenotype of Raf::tor^12D.sev

(Morey et al., 2001)

Sps1^k11320 is a suppressor of photoreceptor cell R7 | ectopic phenotype of Raf::tor^12D.sev

(Morey et al., 2001)

Sps1^k11320 is a suppressor of photoreceptor cell R7 phenotype of Raf::tor^12D.sev

(Morey et al., 2001)

Sps1^k11320 is a suppressor of eye phenotype of sev^S11.Tag:MYC

(Morey et al., 2001)

Sps1^k11320 is a suppressor of ommatidium phenotype of sev^S11.Tag:MYC

(Morey et al., 2001)

Sps1^k11320 is a suppressor of photoreceptor cell R7 | ectopic phenotype of sev^S11.Tag:MYC

(Morey et al., 2001)

Sps1^k11320 is a suppressor of photoreceptor neuron phenotype of sev^S11.Tag:MYC

(Morey et al., 2001)

Sps1^k11320/SelD[+] is a suppressor of wing vein L3 phenotype of Raf^F20.UAS, Scer\GAL4⁶⁰⁴

(Morey et al., 2001)

Sps1^k11320/SelD[+] is a suppressor of wing vein L5 phenotype of Raf^F20.UAS, Scer\GAL4⁶⁰⁴

(Morey et al., 2001)

Sps1^k11320/SelD[+] is a suppressor of wing phenotype of Raf^F20.UAS, Scer\GAL4⁶⁰⁴

(Morey et al., 2001)

NOT Suppressor of

Statement

Reference

Sps1^k11320 is a non-suppressor of eye phenotype of Ras64B^V12.sev

(Morey et al., 2001)

Sps1^k11320 is a non-suppressor of eye phenotype of rl^Sem

(Morey et al., 2001)

Sps1^k11320 is a non-suppressor of photoreceptor neuron phenotype of rl^Sem

(Morey et al., 2001)

Sps1^k11320 is a non-suppressor of wing vein | ectopic phenotype of rl^Sem

(Morey et al., 2001)

Sps1^k11320 is a non-suppressor of interommatidial bristle phenotype of wg^hs.2sev

(Morey et al., 2001)

Sps1^k11320 is a non-suppressor of ommatidium phenotype of wg^hs.2sev

(Morey et al., 2001)

Sps1^k11320 is a non-suppressor of eye phenotype of wg^hs.2sev

(Morey et al., 2001)

Sps1^k11320 is a non-suppressor of photoreceptor neuron phenotype of Ras64B^V12.sev

(Morey et al., 2001)

Sps1^k11320 is a non-suppressor of eye phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^GMR.PF

(Morey et al., 2001)

Sps1^k11320 is a non-suppressor of wing phenotype of Scer\GAL4⁶⁰⁴, tkv^{Q253D.UAS.cLa}

(Morey et al., 2001)

Sps1^k11320 is a non-suppressor of wing vein | ectopic phenotype of Scer\GAL4⁶⁰⁴, tkv^{Q253D.UAS.cLa}

(Morey et al., 2001)

Sps1^k11320 is a non-suppressor of eye phenotype of InR^UAS.cHa, Scer\GAL4^GMR.PF

(Morey et al., 2001)

Sps1^k11320 is a non-suppressor of wing vein L4 phenotype of N^Ax-1

(Morey et al., 2001)

Sps1^k11320 is a non-suppressor of wing vein L5 phenotype of N^Ax-1

(Morey et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Sps1^k07214

(BDGP Project Members, 1994-1999)

Sps1^k12303

(BDGP Project Members, 1994-1999)

Rescued by

Sps1^k11320 is rescued by Sps1^UAS.cAa/Scer\GAL4^69B

(Alsina et al., 1998)

Partially rescued by

Sps1^k11320 is partially rescued by Sps1^UAS.cAa/Scer\GAL4^GMR.PF

(Morey et al., 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

102800

y¹ w^67c23 ; P{lacW}Sps1^k11320 / CyO

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Reversion of the P{lacW} insertion reveals the element is responsible for the mutant phenotype.

(Alsina et al., 1998)

Complements: Hsc70-5^k04907. Complements: Hsc70-5^k06618. Complements: l(2)s3475^s3475.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

113/20

(Alsina et al., 1998, Roch et al., 1998)

SelD^K11320

(Swanson et al., 2009)

SelD^k11320

SelD^ptuf

(Morey et al., 2001)

Sps1^k11320

l(2)k11320

(Morey et al., 2001, Alsina et al., 1999, Spradling et al., 1999)

l(2)k12303^k11320

ptuf¹

(Alsina et al., 1998)

selD^ptuf

(Morey et al., 2003, Morey et al., 2002, Morey et al., 2001)

selD^selD

(Morey et al., 2003)

Name Synonyms

patufet

(Morey et al., 2001)

Secondary FlyBase IDs

References (12)

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