FB2025_01 , released February 20, 2025
Allele: Dmel\Fatp1k10307
Open Close
General Information
Symbol
Dmel\Fatp1k10307
Species
D. melanogaster
Name
FlyBase ID
FBal0064213
Feature type
allele
Associated gene
Dmel\Fatp1
Associated Insertion(s)
P{lacW}Fatp1k10307
Carried in Construct
Also Known As
l(2)k10307, fatpk10307, dFatpK10307
Key Links
Genomic Maps

Allele class

hypomorphic allele - molecular evidence

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Dourlen et al., 2012)

hypomorphic allele - molecular evidence

(Sujkowski et al., 2012)
Mutagen
P-element activity
(BDGP Project Members, 1994-1999)
Progenitor genotype
Associated Insertion(s)
P{lacW}Fatp1k10307
Cytology
Description
Allele components
Component
Use(s)
Inserted element
P{lacW}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      embryonic/larval midgut
      (Dourlen et al., 2012)
      embryonic/larval proventriculus | posterior | restricted
      (Dourlen et al., 2012)
      adult stage
      eye photoreceptor cell
      (Dourlen et al., 2012)
      secondary pigment cell
      (Dourlen et al., 2012)
      tertiary pigment cell
      (Dourlen et al., 2012)
      adult optic lobe | restricted
      (Dourlen et al., 2012)
      Additional Information
      Statement
      Reference

      Fatp1k10307 drives expression in the secondary and tertiary pigment cells, and in neurons in the adult retina. Expression is also detected at the level of the photoreceptors and in cells around the medulla and lamina.

      (Dourlen et al., 2012)
       
      Marker for
      Reflects expression of
      Fatp1
      (Dourlen et al., 2012)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous clones in the retina show progressive loss of photoreceptor cells in the adult. This defect is cell-autonomous. Significantly greater photoreceptor loss is seen in flies reared in normal light conditions compared to those reared in complete darkness.

      The amplitude of the electroretinogram is increased in 8 day old adults containing homozygous retinas compared to that of control flies.

      (Dourlen et al., 2012)

      Heterozygous flies have significantly increased triglyceride and glycogen levels compared to age-matched control flies. Free fatty acid levels in the circulating hemolymph are significantly increased in heterozygous males compared to wild type.

      Heterozygous flies show a significantly reduced feeding rate compared to controls.

      Heterozygous females show an increase in lifespan compared to controls in two or three genetic backgrounds tested.

      Heterozygous males and females aged on high-yeast or high-fatty acid (palmitic acid) diets live longer than controls.

      Heterozygous males show significantly increased resistance to chronic heat stress (28[o]C) compared to controls. Heterozygous females, but not heterozygous males show significantly increased resistance to cold stress (4[o]C) compared to controls.

      Heterozygous males and females show significantly increased resistance to oxidative stress (paraquat) compared to controls.

      Heterozygous males and females show significantly increased resistance to starvation conditions compared to controls.

      The rate of cardiac failure in response to pacing stress is significantly higher across ages in heterozygous adults compared to controls. The frequency of the heart rate is increased across ages in heterozygous flies compared to controls, and the mutant hearts show a reduction in fractional shortening.

      Heterozygous flies show a significantly slower age-related decline in negative geotaxis ability compared to controls. Heterozygous males show a significant degree of improvement in negative geotaxis following a 3-week training period of endurance exercise, with the improvement being similar in magnitude to wild type.

      The increased triglyceride levels seen in heterozygous flies are somewhat reduced by exercise training, although not to wild-type levels. The reduced feeding rate of heterozygous flies is unaffected by exercise. Exercise training of heterozygous flies results in a significant rescue of the fractional shortening defect of the heart, and the increased frequency of the heart rate seen in the mutant flies is also reduced.

      (Sujkowski et al., 2012)

      Homozygous clones in the retina show loss of photoreceptor cells.

      (Gambis et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Fatp1k10307/Fatp[+] is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of MarfB

      (Liu et al., 2017)

      Fatp1k10307/Fatp[+] is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of sicilyE

      (Liu et al., 2017)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Fatp1k10307 has eye photoreceptor cell | somatic clone | cell autonomous | adult stage | conditional phenotype, suppressible by ninaEG69D/ninaE[+]

      (Dourlen et al., 2012)

      Fatp1k10307 has eye photoreceptor cell | somatic clone | cell autonomous | adult stage | conditional phenotype, suppressible by ninaE17/ninaE[+]

      (Dourlen et al., 2012)

      Fatp1k10307 has eye photoreceptor cell | somatic clone | cell autonomous | adult stage | conditional phenotype, suppressible by Arr23

      (Dourlen et al., 2012)

      Fatp1k10307 has eye photoreceptor cell | somatic clone phenotype, suppressible by BacA\p35GMR.PH

      (Gambis et al., 2011)
      NOT Enhancer of
      Suppressor of
      Statement
      Reference

      Fatp1k10307/Fatp[+] is a suppressor of photoreceptor | somatic clone | adult stage | progressive phenotype of MarfB

      (Liu et al., 2017)

      Fatp1k10307/Fatp[+] is a suppressor of photoreceptor | somatic clone | adult stage | progressive phenotype of sicilyE

      (Liu et al., 2017)
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The photoreceptor degeneration observed in adult flies bearing ey>flip whole-eye clones mutant for either sicilyE or MarfB is improved by Fatpk10307 heterozygosity.

      (Liu et al., 2017)

      The presence of ninaEG69D/+ or of ninaE17/+ rescues the photoreceptor degeneration phenotype seen in Fatpk10307 clones in the retina.

      The photoreceptor degeneration phenotype seen in Fatpk10307 clones in the retina is suppressed if the retinas are also mutant for Arr23.

      (Dourlen et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of BacA\p35GMR.PH suppresses the loss of photoreceptor cells which is seen in homozygous Fatpk10307 clones in the retina.

      (Gambis et al., 2011)
      Complementation and Rescue Data
      Fails to complement

      Fatp1k01110a

      (BDGP Project Members, 1994-1999)

      Fatp1k10801

      (BDGP Project Members, 1994-1999)
      Rescued by

      Fatp1k10307 is rescued by Fatp1UAS.cDa/Scer\GAL4ninaE.PT

      (Dourlen et al., 2012)
      Comments

      Expression of FatpScer\UAS.cDa under the control of Scer\GAL4ninaE.PT fully rescues the photoreceptor degeneration phenotype seen in Fatpk10307 clones in the retina.

      (Dourlen et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      I. Kiss.

      (BDGP Project Members, 1994-1999)
      Comments
      Comments

      Complements: RpS27A04820. Complements: l(2)k02605k02605. Complements: l(2)k03906ak03906a. Complements: l(2)k09015ak09015a. Complements: l(2)k09116k09116. Complements: UbcD2k13206.

      (BDGP Project Members, 1994-1999)

      Precise excision of the insertion reverts the mutant phenotype.

      (Sujkowski et al., 2012)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (7)
      References (15)