FB2025_01 , released February 20, 2025
Allele: Dmel\Rab5k08232
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General Information
Symbol
Dmel\Rab5k08232
Species
D. melanogaster
Name
FlyBase ID
FBal0064333
Feature type
allele
Associated gene
Dmel\Rab5
Associated Insertion(s)
P{lacW}Rab5k08232
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(BDGP Project Members, 1994-1999)
Progenitor genotype
Associated Insertion(s)
P{lacW}Rab5k08232
Cytology
Description

Rab5k08232 is a P-element insertion within the 5' leader coding region of Rab5.

(Wucherpfennig et al., 2003)
Allele components
Component
Use(s)
Inserted element
P{lacW}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      embryonic/larval crystal cell

      (Milchanowski et al., 2004)

      synaptic vesicle & larval neuromuscular junction

      (Wucherpfennig et al., 2003)
      Detailed Description
      Statement
      Reference

      The average number of crystal cells per embryo is reduced (to 11.4) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average).

      (Milchanowski et al., 2004)

      Homozygous Rab5k08232 animals die during late second and early third instar larval stages with only a light locomotion phenotype. Consistent with a mild phenotype, both synaptic uptake and transmission rates to the muscles are normal. If raised under `intensive care' conditions, very few Rab5k08232 larvae develop into adult flies, which display a flightless phenotype.

      (Wucherpfennig et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      There is no significant difference in the nervous system degeneration seen in SNF4Aγloe mutants in a Rab5k08230 heterozygous background.

      (Cook et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      Rab5k08230

      (BDGP Project Members, 1994-1999)
      Rescued by

      Rab5k08232 is rescued by Scer\GAL4elav-C155/Rab5UAS.EGFP

      (Wucherpfennig et al., 2003)

      Rab5k08232 is rescued by Rab5+tWa

      (Wucherpfennig et al., 2003)
      Comments

      The Rab5k08232 lethality and phenotype are caused by reduced Rab5 levels in the nervous system as they can be rescued by restricted expression of Rab5Scer\UAS.T:Avic\GFP-EGFP, under the regulation of Scer\GAL4elav-C155 in the nervous system. Rab5+tWa rescues the lethality due to Rab5k08232.

      (Wucherpfennig et al., 2003)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      I. Kiss.

      (BDGP Project Members, 1994-1999)
      Comments
      Comments

      Complements: l(2)0023100231.

      (Misra, 2000.8.25)

      Lethality can be reverted by precise excision P{lacW}Rab5k08232.

      (Wucherpfennig et al., 2003)

      Complements: l(2)0023100231. Complements: l(2)s5379s5379.

      (BDGP Project Members, 1994-1999)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (6)
      Reported As
      Symbol Synonym
      P10786
      (Milchanowski et al., 2004)
      Rab51
      (Wucherpfennig et al., 2003)
      Rab5k08232
      (Wan et al., 2013, Christensen et al., 2008.4.15)
      l(2)k08232
      (Misra, 2000.8.25, Spradling et al., 1999)
      l(2)k08232k08232
      (BDGP Project Members, 1994-1999)
      rab5k08232
      (Chance and Bashaw, 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (12)