FlyBase Allele Report: Dmel\step[k08110]

General Information

Symbol

Dmel\step^k08110

Species

D. melanogaster

Name

FlyBase ID

FBal0064345

Feature type

allele

Associated gene

Dmel\step

Associated Insertion(s)

P{lacW}step^k08110

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}step^k08110

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:21,756,826..21,756,826 [+]

(Parrish et al., 2009, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryo | embryonic stage 5 (with step^SH0323)

(Lee and Harris, 2013)

embryonic/larval crystal cell

(Milchanowski et al., 2004)

embryonic/larval salivary gland

(Fuss et al., 2006)

embryonic epidermis (with Df(2L)BSC150)

(West et al., 2017)

embryonic epidermis (with step^KG09493)

(West et al., 2017)

epithelial cell | embryonic stage (with step^KG09493)

(West et al., 2017)

furrow canal (with step^SH0323)

(Lee and Harris, 2013)

head | embryonic stage (with step^KG09493)

(West et al., 2017)

photoreceptor neuron

(Hahn et al., 2013)

wing

(Hahn et al., 2013)

wing vein

(Hahn et al., 2013)

Detailed Description

Statement

Reference

A small but significant proportion of step^k08110/step^KG09493 transheterozygous embryos exhibit lethality, and a large proportion of these also exhibit epidermal defects, including head scars and a "head hole" phenotype. Prior to germband retraction, these mutants display an apparently normal columnar epithelium and apparently normal mitotic domains that expand the epidermal cell population. During germband retraction in these mutants, a large proportion of epidermal cells exhibit apical elongation, while the non-apical elongating cells are frequently clustered in long-lived rosette-like structures, as compared to controls; laser ablation of either myosin cables (labeled by fluorescent Zipper) of adherens junctions (labeled by fluorescent E-Cadherin) in these epidermal cells leads to significantly faster instantaneous recoils than in controls. During mid-dorsal closure in these mutants, the pulsatile actomyosin networks in amnioserosa cells are indistinguishable from controls.

During mid-dorsal closure, step^k08110/Df(2L)BSC150 transheterozygous embryos exhibit a significant increase in the leading-edge cell contact lengths and a frequent clustering in long-lived multi-cellular rosettes, as compared to controls.

(West et al., 2017)

Late step^k08110 homozygous pupae display photoreceptor loss.

Expression of step^Scer\UAS.FL under the control of Scer\GAL4^{Act5C.Switch.PR} rescues hypomorphic step^k08110 mutants to adulthood.

Expression of step^Scer\UAS.FL via Scer\GAL4^hs.PB rescues the wing defects found in homozygous step^k08110 mutants. Depending on the duration of the heat shock, rescue is either partial or complete.

(Hahn et al., 2013)

Embryos derived from mothers maternally mutant for step^SH0323/step^k08110 have largely lost the endocytic tubules usually seen emanating from early cellularisation furrow canals.

(Lee and Harris, 2013)

Grp1^k08110/Grp1^SH0323 male and female adults are significantly smaller and show a drastic decrease in weight compared to control animals, although the body proportions of the mutant animals are not altered compared to wild type. Homozygous and Grp1^k08110/Grp1^SH0323 larvae are also significantly smaller than controls.

Homozygous third instar larvae show a decrease in endoreduplication activity in the salivary glands, and the size and total number of salivary gland cells is reduced compared to wild type.

A small proportion of homozygotes show embryonic lethality.

(Fuss et al., 2006)

The average number of crystal cells per embryo is reduced (to 17.1) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average).

(Milchanowski et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

step^KG09493/step^k08110 has some die during embryonic stage phenotype, suppressible | partially by zip[+]/zip¹

(West et al., 2017)

step^KG09493/step^k08110 has some die during embryonic stage phenotype, suppressible | partially by zip²/zip[+]

(West et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

step^KG09493/step^k08110 has embryonic epidermis phenotype, enhanceable by shg[+]/shg^R69

(West et al., 2017)

step^KG09493/step^k08110 has head | embryonic stage phenotype, enhanceable by shg[+]/shg^R69

(West et al., 2017)

Suppressed by

Statement

Reference

step^KG09493/step^k08110 has head | embryonic stage phenotype, suppressible | partially by zip[+]/zip¹

(West et al., 2017)

step^KG09493/step^k08110 has head | embryonic stage phenotype, suppressible | partially by zip²/zip[+]

(West et al., 2017)

step^KG09493/step^k08110 has embryonic epidermis phenotype, suppressible | partially by zip[+]/zip¹

(West et al., 2017)

step^SH0323/step^k08110 has embryo | embryonic stage 5 phenotype, suppressible by Rho1^72O/Rho1[+]

(Lee and Harris, 2013)

step^SH0323/step^k08110 has furrow canal phenotype, suppressible by Rho1^72O/Rho1[+]

(Lee and Harris, 2013)

Enhancer of

Statement

Reference

step[+]/step^k08110 is an enhancer of photoreceptor neuron phenotype of S^IIN

(Hahn et al., 2013)

NOT Suppressor of

Statement

Reference

step^SH0323/step^k08110 is a non-suppressor of phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Fuss et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The increased embryonic lethality of step^k08110/step^KG09493 transheterozygotes and associated "head hole" phenotype are partially rescued by heterozygosity for either zip¹ or zip²; conversely, this "head hole" phenotype is severely enhanced by heterozygosity for shg^R69, which results in more frequent and wider head holes, often involving merged head and dorsal holes. The epidermal rosette-like structures during germband retraction of step^k08110/step^KG09493 transheterozygous embryos are also partially suppressed by heterozygosity for zip¹.

(West et al., 2017)

S^IIN/step^k08110 trans-heterozygotes exhibit a photoreceptor differentiation phenotype, increased in severity from S^IIN heterozygotes.

(Hahn et al., 2013)

Maternal heterozygosity for Rho1^72O almost completely suppresses the furrow canal expansion phenotype seen in step^SH0323/step^k08110 mutant embryos.

Maternal heterozygosity for step^k08110 enhances the furrow canal phenotype seen in embryos derived from mothers expressing AP-2α^HMS00653 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}.

(Lee and Harris, 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

step^k11104

(BDGP Project Members, 1994-1999)

Rescued by

step^k08110 is rescued by Scer\GAL4^{Act5C.Switch.PR}/step^UAS.FL

(Hahn et al., 2013)

step^k08110 is rescued by step^UAS.FL/Scer\GAL4^hs.PB

(Hahn et al., 2013)

Comments

Expression of step^Scer\UAS.FL under the control of Scer\GAL4^{Act5C.Switch.PR} rescues hypomorphic step^k08110 mutants to adulthood.

Expression of step^Scer\UAS.FL via Scer\GAL4^hs.PB rescues the wing defects found in homozygous step^k08110 mutants. Depending on the duration of the heat shock, rescue is either partial or complete.

(Hahn et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

10770

y¹ w^67c23; P{lacW}step^k08110/CyO

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Complements: cul-2⁰²⁰⁷⁴. Complements: l(2)03832a^03832a.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

Grp1^k08110

P10770

(Milchanowski et al., 2004)

l(2)k08110

(Spradling et al., 1999)

l(2)k08110^k08110

step^K08110

(Zheng et al., 2019, West et al., 2017, Lee and Harris, 2013, Becker et al., 2010)

step^K081110

(Zeledon et al., 2019)

step^K0

(West et al., 2017)

step^K

(Hahn et al., 2013)

step^k08110

(Zeledon et al., 2019, Fuss et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (12)

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