FlyBase Allele Report: Dmel\dom[k08108]

General Information

Symbol

Dmel\dom^k08108

Species

D. melanogaster

Name

domino

FlyBase ID

FBal0064346

Feature type

allele

Associated gene

Dmel\dom

Associated Insertion(s)

P{lacW}dom^k08108

Carried in Construct

Also Known As

dom¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Roch et al., 1998, BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}dom^k08108

Cytology

Description

P{lacW} inserted at 3' boundary of first exon, 1bp downstream of the splice site for the first intron, which is non-coding, in the same orientation as the dom transcription unit.

(Ruhf et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:21,323,614..21,323,614 [+]

(Walker et al., 2011, Chen et al., 2008, Kurucz et al., 2003, Ruhf et al., 2001, Spradling et al., 1999, Roch et al., 1998, Braun et al., 1997, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

dom^k08108/dom^k08108 mutants exhibit pupal lethality, and a majority of third instar larvae have necrotic lymph glands.

(Börner and Becker, 2016)

84% of flies expressing dom^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^bbg-C96 in a dom^k08108/+ background show wing nicking.

(Kwon et al., 2013)

At two days after clone induction, the percentage of testes carrying dom^k08108 mutant germline stem cell (GSC) clones is indistinguishable from controls, however this decreases over time and by day 8 no testes that contain dom^k08108 mutant GSCs. Similarly, the proportion of testes with dom^k08108 mutant cyst stem cells (CySCs) progressively drops to 0 by day 15.

(Morillo Prado et al., 2013)

dom^k08108 mutants exhibit melanized lymph glands and a lack of hemocytes.

(Ragab, 2006)

dom^k08108 homozygous female germ-line stem cells are lost from their stem cell niche at the same rate as wild-type female germ-line stem cells. However, 91% of dom³ homozygous clone follicle stem cells are lost from their niche within 17 days, compared to only 37.5% of cells in wild-type control clones. This is not accompanied by any increase in in apoptosis of these cells.

(Xi and Xie, 2005)

Lethality acts in the early pupal stages. Lymph glands are black, no hemocytes and reduced neuroblast region in the larval brain. Mititic defects are not evident in the neuroblasts. Females with germline clones do not lay eggs.

(Ruhf et al., 2001)

Homozygous third instar larvae have a striking absence of circulating hemocytes; only 0 to 10 oversized cells are seen per larva, in contrast to the wild-type number of 1000-3000 circulating hemocytes. In addition, the number of sessile plasmatocytes is significantly reduced compared to wild-type.

Homozygous larvae contain large numbers of microorganisms which can be seen on the epithelium lining the integument and on muscles.

The melanisation in response to pricking with a needle is reduced in intensity compared to wild-type larvae.

Melanisation plaques appear on the surface of homozygous larvae after natural infection by spores of B.bassiana, as are seen in wild-type larvae.

There is not a significant difference in the survival rate between larvae pricked with a sterile needle or a needle coated with Gram-positive or Gram-negative bacteria, however, infection with fungal spores induces over 60% lethality in 48 hours in homozygous larvae.

(Braun et al., 1998)

Lethality occurs during third instar larval or pupal stages. Mutants exhibit visible disc abnormalities: very small or no discs. Clones cannot be induced in wild type discs. In tergites, clones with normal size and but lower than expected frequency are recovered. Clones cannot be recovered in the wing disc.

(Roch et al., 1998)

Homozygous larvae are totally devoid of circulating hemocytes. In favourable conditions these larvae have a prolonged third instar, up to 10 days at 20^oC. They show melanized lymph glands. During third instar the blackening invades the whole lobe, extending to the posterior lobes. Melanized lymph glands eventually detach from the dorsal vessel. Ultrastructural analysis reveals that the prohemocytes in the hematopoetic organ are larger than wild type. Lymph glands are filled with necrotic, melanized cells and cells packed with heterogeneous inclusions indicative of strong resorptive processes. Mutant glands are devoid of differentiating prohemocytes. The embryonic hemocytes are unaffected, but by first instar hemocytes are reduced in number. Mutants are also devoid of imaginal discs, imaginal rings and histoblasts in the gut. The size of the brain is significantly reduced. The domain of brain neuroblasts in the optic lobes is reduced. Residual imaginal disc cells exist in small clusters.

(Braun et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

dom^k08108 has lethal | pupal stage phenotype, non-suppressible by Df(2R)ED3921

(Ragab, 2006)

Suppressor of

Statement

Reference

dom^k08108/l(3)hem² is a suppressor of short lived phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Wu et al., 2017)

dom^k08108/l(3)hem² is a suppressor of abnormal neuroanatomy phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Wu et al., 2017)

dom^k08108/dom[+] is a suppressor of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

dom^k08108 has hemocyte phenotype, non-suppressible by Df(2R)ED3921

(Ragab, 2006)

dom^k08108 has embryonic/larval lymph gland phenotype, non-suppressible by Df(2R)ED3921

(Ragab, 2006)

Enhancer of

Statement

Reference

dom^k08108/dom[+] is an enhancer of testis | somatic clone phenotype of His2Av⁸¹⁰

(Morillo Prado et al., 2013)

dom^k08108/dom[+] is an enhancer of male germline stem cell | somatic clone phenotype of His2Av⁸¹⁰

(Morillo Prado et al., 2013)

dom^k08108 is an enhancer of wing blade phenotype of N^nd-1

(Gause et al., 2006)

dom^k08108/dom[+] is an enhancer of wing margin phenotype of mam^g2.1

(Gause et al., 2006)

Suppressor of

Statement

Reference

dom^k08108/l(3)hem² is a suppressor of adult brain phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Wu et al., 2017)

dom^k08108/l(3)hem² is a suppressor of embryonic/larval plasmatocyte | adult stage phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Wu et al., 2017)

dom^k08108/dom[+] is a suppressor of wing vein L4 phenotype of N^Ax-E2

(Gause et al., 2006)

dom^k08108/dom[+] is a suppressor of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of dom^k08108 enhances the loss of His2Av⁸¹⁰ mutant germline stem cells in the testis. A greater proportion of testes contain His2Av⁸¹⁰ dom^k08108/+ mutant GSC clones than with His2Av⁸¹⁰ alone.

(Morillo Prado et al., 2013)

Heterozygosity for dom^k08108 increases the number of wing margin nicks seen in mam^g2.1 heterozygotes.

Heterozygosity for dom^k08108 further reduces the width of N^nd-1 wings.

Wing vein L4 is shortened in N^Ax-E2 mutants; heterozygosity for dom^k08108 slightly increases the length of L4.

(Gause et al., 2006)

Df(2R)ED3921 fails to suppress the melanized lymph gland and lack of hemocyte phenotype of dom^k08108 mutants .

(Ragab, 2006)

Tl⁸/+ ; dom^k08108, cact⁷ dom^k08108 or hop^Tum ; dom^k08108 double mutant larvae contain melanotic masses, although their frequency is markedly reduced compared to Tl⁸/+, cact⁷ or hop^Tum single mutant larvae and the masses are devoid of hemocytes.

dom^k08108 imd¹ and dom^k08108 Bc¹ double mutant larvae show a dramatically compromised survival after injury (whether it is a clean injury or an injury combined with infection).

(Braun et al., 1998)

Xenogenetic Interactions

Statement

Reference

The presence of both l(3)hem² and dom^k08108 completely suppresses the shortened lifespan and recruitment of plasmatocytes to the postero-dorsal site of the brain near the mushroom body lobe, and partially suppresses the neurodegeneration seen in Ecc15 infected flies that express Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^elav-C155.

(Wu et al., 2017)

Complementation and Rescue Data

Fails to complement

dom^k02704

(BDGP Project Members, 1994-1999)

dom^k00904

(BDGP Project Members, 1994-1999)

dom^k07921

(BDGP Project Members, 1994-1999)

Partially rescued by

dom^k08108 is partially rescued by dom^{Tag:TY1,sfGFP,Tag:FLAG}

(Börner and Becker, 2016)

dom^k08108 is partially rescued by dom^{A.Tag:TY1,sfGFP,Tag:FLAG}

(Börner and Becker, 2016)

dom^k08108 is partially rescued by dom^{B.Tag:TY1,sfGFP,Tag:FLAG}

(Börner and Becker, 2016)

Comments

Expression of dom^{T:Scer\TY1,T:Avic\GFP-SF,T:Zzzz\FLAG}, dom^{A.T:Scer\TY1,T:Avic\GFP-SF,T:Zzzz\FLAG}, or dom^{B.T:Scer\TY1,T:Avic\GFP-SF,T:Zzzz\FLAG} partially rescues the pupal lethality and larval lymph gland necrosis seen in dom^k08108/dom^k08108 mutants.

(Börner and Becker, 2016)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10767

y¹ w^67c23; P{lacW}dom^k08108/CyO

Kyoto

111237

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}dom^k08108 /CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Excision of the P{lacW} can be accompanied by reversion of the mutant phenotype.

(Ruhf et al., 2001)

Complements: Sdc¹⁰⁶⁰⁸.

(BDGP Project Members, 1994-1999)

P-element induced reversion of the mutant phenotype is accompanied by loss of the P{lacW} element.

(Braun et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

81/8

(Roch et al., 1998)

dom¹

(Wu et al., 2017, Börner and Becker, 2016, Kwon et al., 2013, Kirilly et al., 2011, Gause et al., 2006, Ragab, 2006, Rus et al., 2006, Xi and Xie, 2005, Ruhf et al., 2001, Tzou et al., 2000)

dom^K08108

(Davis et al., 2008)

dom^k08108

(Debruhl et al., 2013, Morillo Prado et al., 2013)

domK08108

(Janody and Treisman, 2006)

domino^k08108

(BDGP Project Members, 1994-1999)

l(2)k08108

(Spradling et al., 1999)

l(2)k08108^k08108

Name Synonyms

domino

(Braun et al., 1998)

Secondary FlyBase IDs

References (24)

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