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General Information
Symbol
Dmel\domk08108
Species
D. melanogaster
Name
domino
FlyBase ID
FBal0064346
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dom1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{lacW} inserted at 3' boundary of first exon, 1bp downstream of the splice site for the first intron, which is non-coding, in the same orientation as the dom transcription unit.

Insertion components
P{lacW}domk08108
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

domk08108/domk08108 mutants exhibit pupal lethality, and a majority of third instar larvae have necrotic lymph glands.

84% of flies expressing domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 in a domk08108/+ background show wing nicking.

At two days after clone induction, the percentage of testes carrying domk08108 mutant germline stem cell (GSC) clones is indistinguishable from controls, however this decreases over time and by day 8 no testes that contain domk08108 mutant GSCs. Similarly, the proportion of testes with domk08108 mutant cyst stem cells (CySCs) progressively drops to 0 by day 15.

domk08108 mutants exhibit melanized lymph glands and a lack of hemocytes.

domk08108 homozygous female germ-line stem cells are lost from their stem cell niche at the same rate as wild-type female germ-line stem cells. However, 91% of dom3 homozygous clone follicle stem cells are lost from their niche within 17 days, compared to only 37.5% of cells in wild-type control clones. This is not accompanied by any increase in in apoptosis of these cells.

Lethality acts in the early pupal stages. Lymph glands are black, no hemocytes and reduced neuroblast region in the larval brain. Mititic defects are not evident in the neuroblasts. Females with germline clones do not lay eggs.

Homozygous third instar larvae have a striking absence of circulating hemocytes; only 0 to 10 oversized cells are seen per larva, in contrast to the wild-type number of 1000-3000 circulating hemocytes. In addition, the number of sessile plasmatocytes is significantly reduced compared to wild-type.

Homozygous larvae contain large numbers of microorganisms which can be seen on the epithelium lining the integument and on muscles.

The melanisation in response to pricking with a needle is reduced in intensity compared to wild-type larvae.

Melanisation plaques appear on the surface of homozygous larvae after natural infection by spores of B.bassiana, as are seen in wild-type larvae.

There is not a significant difference in the survival rate between larvae pricked with a sterile needle or a needle coated with Gram-positive or Gram-negative bacteria, however, infection with fungal spores induces over 60% lethality in 48 hours in homozygous larvae.

Lethality occurs during third instar larval or pupal stages. Mutants exhibit visible disc abnormalities: very small or no discs. Clones cannot be induced in wild type discs. In tergites, clones with normal size and but lower than expected frequency are recovered. Clones cannot be recovered in the wing disc.

Homozygous larvae are totally devoid of circulating hemocytes. In favourable conditions these larvae have a prolonged third instar, up to 10 days at 20oC. They show melanized lymph glands. During third instar the blackening invades the whole lobe, extending to the posterior lobes. Melanized lymph glands eventually detach from the dorsal vessel. Ultrastructural analysis reveals that the prohemocytes in the hematopoetic organ are larger than wild type. Lymph glands are filled with necrotic, melanized cells and cells packed with heterogeneous inclusions indicative of strong resorptive processes. Mutant glands are devoid of differentiating prohemocytes. The embryonic hemocytes are unaffected, but by first instar hemocytes are reduced in number. Mutants are also devoid of imaginal discs, imaginal rings and histoblasts in the gut. The size of the brain is significantly reduced. The domain of brain neuroblasts in the optic lobes is reduced. Residual imaginal disc cells exist in small clusters.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference

domk08108 has lethal | pupal stage phenotype, non-suppressible by Df(2R)ED3921

Suppressor of
Statement
Reference
Phenotype Manifest In
NOT suppressed by
Statement
Reference

domk08108 has hemocyte phenotype, non-suppressible by Df(2R)ED3921

Enhancer of
Statement
Reference

domk08108/dom[+] is an enhancer of testis | somatic clone phenotype of His2Av810

domk08108 is an enhancer of wing blade phenotype of Nnd-1

domk08108/dom[+] is an enhancer of wing margin phenotype of mamg2.1

Suppressor of
Statement
Reference

domk08108/dom[+] is a suppressor | partially of wing vein L4 phenotype of NAx-E2

domk08108/dom[+] is a suppressor of eye | heat sensitive phenotype of peb1

Additional Comments
Genetic Interactions
Statement
Reference

One copy of domk08108 enhances the loss of His2Av810 mutant germline stem cells in the testis. A greater proportion of testes contain His2Av810 domk08108/+ mutant GSC clones than with His2Av810 alone.

Heterozygosity for domk08108 increases the number of wing margin nicks seen in mamg2.1 heterozygotes.

Heterozygosity for domk08108 further reduces the width of Nnd-1 wings.

Wing vein L4 is shortened in NAx-E2 mutants; heterozygosity for domk08108 slightly increases the length of L4.

Df(2R)ED3921 fails to suppress the melanized lymph gland and lack of hemocyte phenotype of domk08108 mutants .

Tl8/+ ; domk08108, cact7 domk08108 or hopTum ; domk08108 double mutant larvae contain melanotic masses, although their frequency is markedly reduced compared to Tl8/+, cact7 or hopTum single mutant larvae and the masses are devoid of hemocytes.

domk08108 imd1 and domk08108 Bc1 double mutant larvae show a dramatically compromised survival after injury (whether it is a clean injury or an injury combined with infection).

Xenogenetic Interactions
Statement
Reference

The presence of both l(3)hem2 and domk08108 completely suppresses the shortened lifespan and recruitment of plasmatocytes to the postero-dorsal site of the brain near the mushroom body lobe, and partially suppresses the neurodegeneration seen in Ecc15 infected flies that express Hsap\APPAβ42.Scer\UAS.cBa under the control of Scer\GAL4elav-C155.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Excision of the P{lacW} can be accompanied by reversion of the mutant phenotype.

P-element induced reversion of the mutant phenotype is accompanied by loss of the P{lacW} element.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
References (23)