Allele Dmel\aPKC^k06403

General Information
Symbol	Dmel\aPKC^k06403	Species	D. melanogaster
Name		FlyBase ID	FBal0064438
Feature type	allele	Associated gene	Dmel\aPKC
Also Known As	DaPKC^k06403, aPKC⁰⁶⁴⁰³, l(2)k06403

Map ( GBrowse )	Untitled Document
Allele class	loss of function allele, amorphic allele - genetic evidence
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	amorphic allele - genetic evidence (Rolls et al., 2003) loss of function allele (Ruiz-Canada et al., 2004)
Mutagen	P-element activity (BDGP Project Members, 1994-1999)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 2R:10,840,608..10,840,608 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Wodarz et al., 2000, Ruiz-Canada et al., 2004, Nam and Choi, 2003, Ogawa et al., 2009, Kaplan et al., 2011, Guilgur et al., 2012, Weber et al., 2012)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{lacW} insertion in the third intron. (Wodarz et al., 2000)
Caused by insertion	P{lacW}aPKC^k06403 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Wodarz et al., 2000, Ruiz-Canada et al., 2004, Nam and Choi, 2003, Ogawa et al., 2009, Kaplan et al., 2011, Guilgur et al., 2012, Weber et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	cell lethal \| first instar larval stage \| somatic clone (Guilgur et al., 2012) cell polarity defective (Rolls et al., 2003, Kaplan et al., 2011) cell polarity defective \| somatic clone (Abdelilah-Seyfried et al., 2003) cytokinesis defective \| heat sensitive (with aPKC^ts) (Guilgur et al., 2012) decreased cell number \| somatic clone (Rolls et al., 2003) increased cell death \| second instar larval stage \| somatic clone (Guilgur et al., 2012) increased cell death \| third instar larval stage \| heat sensitive (with aPKC^ts) (Guilgur et al., 2012) lethal (with aPKC^psu265) (Kim et al., 2009) lethal (with aPKC^psu417) (Kim et al., 2009) lethal (with Df(2R)Jp1) (Kim et al., 2009) lethal \| embryonic stage (with Df(2R)Jp1) (Wodarz et al., 2000) lethal \| embryonic stage \| recessive (Wodarz et al., 2000) lethal \| heat sensitive (with aPKC^ts) (Guilgur et al., 2012) lethal \| larval stage (Ruiz-Canada et al., 2004) lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999, Kim et al., 2009) lethal \| recessive \| second instar larval stage (Lee et al., 2006) lethal \| second instar larval stage (Rolls et al., 2003) lethal \| second instar larval stage (with Df(2R)Jp1) (Rolls et al., 2003) locomotor behavior defective \| larval stage (Rolls et al., 2003) locomotor behavior defective \| larval stage (with Df(2R)Jp1) (Rolls et al., 2003) mitotic cell cycle defective (Chabu and Doe, 2008) neuroanatomy defective (Lee et al., 2006) neuroanatomy defective \| somatic clone (Rolls et al., 2003) neurophysiology defective (with aPKC^Exc55) (Ruiz-Canada et al., 2004) partially lethal - majority die (with aPKC^psu69) (Kim et al., 2009) partially lethal - majority die (with aPKC^psu141) (Kim et al., 2009) planar polarity defective (Kaplan et al., 2011) planar polarity defective (with aPKC^ts) (Guilgur et al., 2012) size defective \| heat sensitive (with aPKC^ts) (Guilgur et al., 2012)
Phenotype Manifest In
	bouton (with aPKC^Exc55) (Ruiz-Canada et al., 2004) denticle belt (Kaplan et al., 2011) egg chamber \| germline clone (Cox et al., 2001) embryo \| dorsal closure stage \| heat sensitive (with aPKC^ts) (Guilgur et al., 2012) embryonic/first instar larval cuticle (Wodarz et al., 2000) embryonic/first instar larval cuticle (with Df(2R)Jp1) (Wodarz et al., 2000) embryonic/first instar larval cuticle \| ventral (Wodarz et al., 2000) embryonic/larval cuticle \| germline clone \| maternal effect (Kim et al., 2009) embryonic/larval neuroblast (Chabu and Doe, 2008) embryonic head (Wodarz et al., 2000) epithelial cell (Wodarz et al., 2000) eye disc (Rolls et al., 2003) female germline cyst (with aPKC^ts) (Guilgur et al., 2012) follicle cell \| ectopic \| somatic clone (Abdelilah-Seyfried et al., 2003) follicle cell \| germline clone (Cox et al., 2001) follicle cell \| somatic clone (Abdelilah-Seyfried et al., 2003, Duchi et al., 2010, Kim et al., 2009) larval brain & neuroblast (Lee et al., 2006) microtubule \| somatic clone (Duchi et al., 2010) mushroom body & neuron \| somatic clone (Rolls et al., 2003) nurse cell \| germline clone (Cox et al., 2001) oocyte \| germline clone (Cox et al., 2001, Kim et al., 2009) terminal tracheal cell \| somatic clone (Jones and Metzstein, 2011) wing disc \| third instar larval stage \| heat sensitive (with aPKC^ts) (Guilgur et al., 2012) zonula adherens \| somatic clone (Abdelilah-Seyfried et al., 2003)
Detailed Description
	Statement Reference aPKC[k06403] mutant wing disc clones cannot be detected at the first instar larval stage. However, clones can be seen when induction takes place at the second instar larval stage, and these are positive for apoptosis. aPKC[ts]/aPKC[k06403] flies are viable at the permissive temperature (25[o]C) and adult flies appear morphologically normal. However, females are sterile, with no larvae hatching from laid eggs even when crossed with wild type males. Germline cyst encapsulation phenotypes are seen immediately after the mesenchymal-to-epithelial transition in region 3 of the germarium in aPKC[ts]/Df(2R)l4 mutant ovaries at the permissive temperature. The resulting follicular epithelium adherens junctions, apicobasal polarity and actin-myosin cytoskeleton appear normal. At the restrictive temperature (30[o]C) very few flies eclose from the pupal cases, with occasional escaper flies that exhibit abdominal dorsal closure defects. At 27-28[o]C (the semi-permissive temperature) viability is variable, but almost all flies show significant dorsal closure defects. aPKC[ts]/aPKC[k06403] third instar larval wing discs show a temperature sensitive induction of apoptosis. Whereas at the permissive temperatures (18[o]C and 25[o]C) there are low levels of cell extrusion and apoptosis, at the restrictive temperature (30[0]C) significantly higher levels of cell extrusion and apoptosis are seen, and the wings discs appear smaller than in controls. No phenotypes are seen in the heterozygous controls. At both the permissive and restrictive temperatures there is a significant decrease in the proportion of aPKC[ts]/aPKC[k06403] mutant wing disc cells with the correct planar spindle orientation compared to controls. The decrease is greater at 30[0]C than at 25[o]C. Embryos maternally mutant for aPKC[ts]/aPKC[k06403] fail to complete germ band extension (GBE), with a complete loss of epithelial integrity at the permissive temperature. (Guilgur et al., 2012) Homozygous tracheal terminal cells show a reduction in branching (primarily affecting class II and later-order branches) compared to wild type, a severe reduction in gas-filled lumen and an abnormal branch tip morphology. (Jones and Metzstein, 2011) aPKC[k06403] mutants display a mild defect in denticle belt formation. (Kaplan et al., 2011) Microtubules are disorganised but are not lost in homozygous follicle cell clones. Piling up of follicle cells and stretching or gaps in the epithelium are seen. (Duchi et al., 2010) All aPKC[k06403] mutant adult Malpighian tubule renal and nephric stem cells (RNSCs) differentiate into renalcytes (RCs). (Zeng et al., 2010) Occasional aPKC[psu265]/aPKC[k06403] and aPKC[psu417]/aPKC[k06403] adult escapers are seen. Embryos derived from homozygous female germ-line clones develop only scattered crumbs of cuticle. Epithelial cells in stage 7 embryos derived from homozygous female germ-line clones lose their columnar, monolayered organisation, rounding up and losing contact with each other. Homozygous follicle cell clones show extensive multilayering of the epithelium. An oocyte is specified in only 28% of germ-line clones. (Kim et al., 2009) Homozygous single neuroblast clones which are induced in first instar larvae and allowed to develop for 96 hours always contain a single large dpn-positive neuroblast (as do control single neuroblast clones). Progression through mitosis is delayed in aPKC[k06403] larval neuroblasts compared to wild-type neuroblasts; the transit from nuclear envelope breakdown to anaphase onset is 17.84 +/- 4.52 minutes in the mutant neuroblasts compared to 7.76 +/- 2.04 minutes in wild-type neuroblasts. (Chabu and Doe, 2008) 80% of homozygous female germline clones are arrested at stage 5 or 6 of oogenesis, but some germline clones do develop until stages 9 or 10. (Tian and Deng, 2008) Homozygous larvae have a reduced number of brain neuroblasts. (Lee et al., 2006) The transplantation of aPKC^k06403 larval neuroblast clones into adult hosts does not cause tumor formation. (Caussinus and Gonzalez, 2005) No aPKC^k06403 homozygous clones can be recovered in the eyes of aPKC^k06403 Scer\FRT; Scer\FLP1^ey.PN adults and only a few very small clones can be recovered in the eye imaginal disc. (Djiane et al., 2005) In aPKC^Exc55/aPKC^k06403 mutant animals, there is a decrease in the number of synaptic boutons at the neuromuscular junction (NMJ). Muscle size remains normal. (Ruiz-Canada et al., 2004) When somatic clones are made in the follicular epithelium cell polarity defects are seen. These are more severe at the poles of the egg chamber than in lateral positions where polarity is often normal. Zonula adherens is also compromised in clones. Mutant follicle cells are also multilayered. most strikingly at the anterior and posterior poles. When mutant clones are made in the border cells no migration defects are seen. (Abdelilah-Seyfried et al., 2003) Somatic clones of aPKC^k06403 in the pupal retina are very small - consisting of only a few cells, suggesting a role in proliferation and/or survival of retinal cells. (Nam and Choi, 2003) Stage 15 homozygous embryos have normal epithelial and neuroblast polarity. Homozygous aPKC^k06403 mutants die as late second instar larvae, they remain as second instar larvae for at least twice as long as normal. aPKC^k06403 mutant second larval instar eye imaginal discs are not organised into an epithelial monolayer and appear to have lost epithelial polarity. They are also smaller than in wild-type. aPKC^k06403 mutant clones exhibit a dramatic decrease in the number of neuronal progeny compared to wild-type. aPKC^k06403 mushroom body clones contain 75-125 neurons, consisting primarily of early-born γ neurons (in contrast to wild-type clones, which contain 300-400 neurons, consisting of early-born γ neurons as well as later-born α- and β-neurons). (Rolls et al., 2003) aPKC^k06403 germ-line clones fail to differentiate an oocyte as indicated by the presence of polyploid nurse cell nuclei in germ-line mutant egg chambers. Depletion of aPKC function from the follicle cells leads to their multilayering, which disrupts the normal partitioning of germ-line nuclei to successively mature egg chambers caused by mispositioning of mutant follicle cells. IN aPKC^k06403 mutant germ-line cysts ring canal distribution and spatioal distribution are indistinguishable from wild-type. No defects are seen in fusome morphology. (Cox et al., 2001) Homozygous aPKC^k06403 and aPKC^k06403/Df(2R)Jp1 embryos derived from heterozygous aPKC^k06403/+ females do not form cuticle because they die before cuticle secretion begins (most die before or during cellularisation). The embryos show multilayering of the epithelium at gastrulation, the cells are extremely irregular in shape and apico-basal polarity of the epithelium is lost. Embryos that contain a zygotic wild-type allele of aPKC and are derived from aPKC^k06403/+ females often die with characteristic head defects. At the anterior tip of the embryo, the epithelium is multilayered. Hemizygous embryos (that have a wild-type maternal contribution of aPKC) do not show obvious defects before germ band extension. The embryos form cuticles with severe head defects and large ventral holes, or lack ventral cuticle altogether. (Wodarz et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference aPKC^k06403, arm^F1α has cell polarity defective phenotype, enhanceable by sgg^M11 (Kaplan et al., 2011) aPKC^k06403, arm^F1α has planar polarity defective phenotype, enhanceable by sgg^M11 (Kaplan et al., 2011) aPKC^k06403 has cell polarity defective phenotype, enhanceable by sgg^M11/arm^F1α (Kaplan et al., 2011) aPKC^k06403 has planar polarity defective phenotype, enhanceable by sgg^M11/arm^F1α (Kaplan et al., 2011)
Suppressed by
Statement Reference aPKC^k06403 has planar polarity defective phenotype, suppressible by arm^F1α (Kaplan et al., 2011) aPKC^ts/aPKC^k06403 has increased cell death \| heat sensitive phenotype, suppressible by raps[+]/raps¹⁹³ (Guilgur et al., 2012)
NOT suppressed by
Statement Reference aPKC^k06403 has cell polarity defective phenotype, non-suppressible by arm^F1α (Kaplan et al., 2011)
Enhancer of
Statement Reference arm^F1α/aPKC^k06403 is an enhancer of cell polarity defective phenotype of sgg^M11 (Kaplan et al., 2011) arm^F1α/aPKC^k06403 is an enhancer of planar polarity defective phenotype of sgg^M11 (Kaplan et al., 2011) sgg^M11/aPKC^k06403 is an enhancer of cell polarity defective phenotype of arm^F1α (Kaplan et al., 2011) sgg^M11/aPKC^k06403 is an enhancer of planar polarity defective phenotype of arm^F1α (Kaplan et al., 2011)
Suppressor of
Statement Reference aPKC^k06403/aPKC[+] is a suppressor \| partially of increased cell number \| somatic clone phenotype of tws^j11C8 (Chabu and Doe, 2009) aPKC^k06403/aPKC[+] is a suppressor \| partially of neuroanatomy defective \| adult stage phenotype of Gl^Δ.Scer\UAS, Scer\GAL4^A307 (Ma et al., 2009) aPKC^k06403/aPKC[+] is a suppressor of neuroanatomy defective \| larval stage phenotype of l(2)gl³³⁴/l(2)gl³⁶⁴⁴ (Haenfler et al., 2012) aPKC^k06403/aPKC[+] is a suppressor of neuroanatomy defective \| somatic clone phenotype of Zif^1L15 (Chang et al., 2010) aPKC^k06403/aPKC[+] is a suppressor of visible \| heat sensitive phenotype of peb¹ (Wilk et al., 2004)
NOT Suppressor of
Statement Reference aPKC^k06403 is a non-suppressor of hyperplasia \| conditional \| somatic clone phenotype of Ras85D^V12.Scer\UAS, Scer\GAL4^{Scer\FRT.Act5C}, Scer\GAL80^αTub84B.PL (Zeng et al., 2010)
Other
Statement Reference aPKC^k06403, arm^F1α has cell polarity defective phenotype (Kaplan et al., 2011)
Phenotype Manifest In
Enhanced by
Statement Reference aPKC^k06403, arm^F1α has denticle belt phenotype, enhanceable by sgg^M11 (Kaplan et al., 2011) aPKC^k06403 has denticle belt phenotype, enhanceable by sgg^M11/arm^F1α (Kaplan et al., 2011)
Suppressed by
Statement Reference aPKC^k06403 has denticle belt phenotype, suppressible by arm^F1α (Kaplan et al., 2011) aPKC^ts/aPKC^k06403 has embryo \| dorsal closure stage \| heat sensitive phenotype, suppressible by raps[+]/raps¹⁹³ (Guilgur et al., 2012) aPKC^ts/aPKC^k06403 has wing disc \| third instar larval stage \| heat sensitive phenotype, suppressible by raps[+]/raps¹⁹³ (Guilgur et al., 2012)
Enhancer of
Statement Reference aPKC^k06403/aPKC[+] is an enhancer of embryonic/larval cuticle phenotype of baz⁴ (Shao et al., 2010) aPKC^k06403 is an enhancer of neuroblast \| third instar larval stage phenotype of l(2)gl⁴ (Rolls et al., 2003) arm^F1α/aPKC^k06403 is an enhancer of denticle belt phenotype of sgg^M11 (Kaplan et al., 2011) sgg^M11/aPKC^k06403 is an enhancer of denticle belt phenotype of arm^F1α (Kaplan et al., 2011)
Suppressor of
Statement Reference aPKC^k06403/aPKC[+] is a suppressor \| partially of embryonic/larval brain \| third instar larval stage phenotype of l(2)gl³³⁴/l(2)gl⁴ (Rolls et al., 2003) aPKC^k06403/aPKC[+] is a suppressor \| partially of embryonic/larval neuroblast \| supernumerary \| somatic clone phenotype of tws^j11C8 (Chabu and Doe, 2009) aPKC^k06403/aPKC[+] is a suppressor \| partially of embryonic/larval neuroblast \| supernumerary phenotype of Df(3R)Exel6265/tws^j11C8 (Chabu and Doe, 2009) aPKC^k06403/aPKC[+] is a suppressor \| partially of embryonic/larval optic lobe phenotype of Df(3R)Exel6265/tws^j11C8 (Chabu and Doe, 2009) aPKC^k06403/aPKC[+] is a suppressor \| partially of eye phenotype of Fmr1^sev.PW (Zarnescu et al., 2005) aPKC^k06403/aPKC[+] is a suppressor \| partially of giant fiber neuron phenotype of Gl^Δ.Scer\UAS, Scer\GAL4^A307 (Ma et al., 2009) aPKC^k06403/aPKC[+] is a suppressor \| partially of neuroblast & larval brain & third instar larva phenotype of l(2)gl³³⁴ (Rolls et al., 2003) aPKC^k06403/aPKC[+] is a suppressor \| partially of ommatidium phenotype of Fmr1^sev.PW (Zarnescu et al., 2005) aPKC^k06403/aPKC[+] is a suppressor of dorsal mesothoracic disc & epithelial cell phenotype of l(2)gl³³⁴ (Rolls et al., 2003) aPKC^k06403/aPKC[+] is a suppressor of eye \| heat sensitive phenotype of peb¹ (Wilk et al., 2004) aPKC^k06403/aPKC[+] is a suppressor of neuroblast \| supernumerary \| somatic clone phenotype of Zif^1L15 (Chang et al., 2010) aPKC^k06403/aPKC[+] is a suppressor of NMJ bouton \| larval stage phenotype of Fmr1³ (Zarnescu et al., 2005) aPKC^k06403/aPKC[+] is a suppressor of secondary neuroblast \| supernumerary \| larval stage phenotype of l(2)gl³³⁴/l(2)gl³⁶⁴⁴ (Haenfler et al., 2012) aPKC^k06403/aPKC[+] is a suppressor of synapse & larval neuromuscular junction phenotype of Fmr1³ (Zarnescu et al., 2005) aPKC^k06403/aPKC[+] is a suppressor of type II neuroblast \| supernumerary \| larval stage phenotype of l(2)gl³³⁴/l(2)gl³⁶⁴⁴ (Haenfler et al., 2012) aPKC^k06403/aPKC[+] is a suppressor of ventral thoracic disc & epithelial cell phenotype of l(2)gl³³⁴ (Rolls et al., 2003) aPKC^k06403/aPKC[+] is a suppressor of ventral thoracic disc phenotype of l(2)gl³³⁴ (Rolls et al., 2003) aPKC^k06403/aPKC[+] is a suppressor of wing disc phenotype of l(2)gl³³⁴ (Rolls et al., 2003) aPKC^k06403 is a suppressor of larval brain & neuroblast \| supernumerary phenotype of l(2)gl³³⁴ (Lee et al., 2006)
NOT Suppressor of
Statement Reference aPKC^k06403 is a non-suppressor of adult Malpighian tubule \| conditional \| somatic clone phenotype of Ras85D^V12.Scer\UAS, Scer\GAL4^{Scer\FRT.Act5C}, Scer\GAL80^αTub84B.PL (Zeng et al., 2010)
Other
Statement Reference aPKC^k06403, arm^F1α has denticle belt phenotype (Kaplan et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference raps[193] dominantly suppresses the temperature sensitive induction of apoptosis seen in aPKC[ts]/aPKC[k06403] third instar larval wing discs at the semi-permissive temperature (28[o]C). However, this suppression does not occur at the restrictive temperature (30[o]C). raps[193] dominantly suppresses the abdominal dorsal closure defects seen in aPKC[ts]/aPKC[k06403] mutant flies at the semi-permissive temperature (28[o]C). However, this suppression did not occur at the restrictive temperature (30[o]C). (Guilgur et al., 2012) aPKC[k06403]/+ suppresses the increased number of type II neuroblasts and intermediate neural progenitor cells seen in l(2)gl[334]/l(2)gl[3644] larval brains. (Haenfler et al., 2012) arm[F1α]; aPKC[k06403] double mutants maintain some apical-basal polarity patterning, and while cell shapes are somewhat disrupted, cells appear to align in rows. arm[F1α]; sgg[M11]; aPKC[k06403] triple mutants exhibit significant denticle and cell disruption. Specifically, proper cell shapes and cell alignment are lost, and instead of organization into even rows, cells curved and formed denticle swirls. (Kaplan et al., 2011) The neuroblast overgrowth phenotype seen in Zif[1L15] mutant clones is significantly suppressed in a aPKC[k06403] heterozygous background. (Chang et al., 2010) The cuticle phenotype of dead embryos derived from baz[4]/+ embryos derived from a cross of baz[4]/+ females to wild-type males is enhanced if the females also carry one copy of aPKC[k06403]. (Shao et al., 2010) Hyperplasia in adult Malpighian tubule clones expressing Ras85D[V12.Scer\UAS] (using the MARCM system, under the control of Scer\GAL80[αTub84B.PL] and Scer\GAL4[Scer\FRT.Act5C]) is not suppressed by making them also mutant for aPKC[k06403]. (Zeng et al., 2010) Larval tws[j11C8] mutant clones in an aPKC[k06403]/+ background contain an intermediate number of neuroblasts per clone, partially rescuing the single cell tws[j11C8] clone phenotype. The third instar optic lobe of tws[j11C8]/Df(3R)Exel6265 in a aPKC[k06403]/+ background have a partially reduced number of optic lobe neuroblasts, partially rescuing the tws[j11C8]/Df(3R)Exel6265 phenotype. (Chabu and Doe, 2009) The giant fiber axon defects seen in adults expressing Gl[Δ.Scer\UAS] under the control of Scer\GAL4[A307] are partially suppressed by aPKC[k06403]/+. (Ma et al., 2009) The reduced size seen in l(2)gl⁴ L3 larval neuroblasts is not suppressed by aPKC^k06403/aPKC^k06403 and may in fact be slightly enhanced. l(2)gl³³⁴ aPKC^k06403/l(2)gl³³⁴ + larvae exhibit essentially normal leg and wing imaginal disc morphology including disc size, columnar cell shape and an approximately normal apical membrane domain, at the early third instar stage. The increase in L3 larval brain volume seen in l(2)gl³³⁴/l(2)gl⁴ animals is partially suppressed by aPKC^k06403/+. The increase in neuroblast number seen in homozygous l(2)gl³³⁴ third larval instar brains is partially suppressed by aPKC^k06403/+. aPKC^k06403 heterozygosity cannot suppress the l(2)gl⁴ "small neuroblast" phenotype. (Rolls et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	aPKC^SG58 (Ma et al., 2009)
Rescued by	aPKC^k06403 is rescued by aPKC^{S330A.T422A.Scer\UAS}/Scer\GAL4^da.G32 (Kaplan et al., 2011) aPKC^k06403 is rescued by aPKC^Scer\UAS.cBa/Scer\GAL4^da.G32 (Kaplan et al., 2011) aPKC^k06403 is rescued by Scer\GAL4^da.G32/aPKC^ΔN.Scer\UAS (Kaplan et al., 2011)
Comments
Stocks ( 2 )
Bloomington	10622 y¹ w^67c23; P{lacW}aPKC^k06403/CyO
Kyoto	111194 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}aPKC^k06403 /CyO y⁺
Notes on Origin
Discoverer	I. Kiss.

Comments
	Excision of the P{lacW} element can revert the lethality of aPKC^k06403, indicating that the P{lacW} insertion is responsible for the aPKC^k06403 phenotype. (Wodarz et al., 2000)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	aPKC⁰⁶⁴⁰³ (Wang et al., 2009, Ma et al., 2009, Chang et al., 2010, Haenfler et al., 2012) aPKC⁶⁴⁰³ (Fletcher et al., 2012) aPKC^k0643 (Chabu and Doe, 2008) aPKC^K06304 (Nam and Choi, 2003) aPKC^k06403 (Djiane et al., 2005, Christensen et al., 2007.10.29, Caussinus and Gonzalez, 2005, Lee et al., 2006, Jones and Metzstein, 2011, Christensen et al., 2008.10.20, Chabu and Doe, 2008, Chabu and Doe, 2009, Lee et al., 2006, Duchi et al., 2010, Kaplan et al., 2011, Kim et al., 2009) aPKC^K06403 (Shao et al., 2010, Morais-de-Sá et al., 2010, Kaplan et al., 2009, Leibfried et al., 2013) apkc^K06403 (Tian and Deng, 2008) apkc^k06403 (Guilgur et al., 2012) aPKC^l(2)k06403 (Wang and Riechmann, 2007) aPKC-zeta^k06403 (Zarnescu et al., 2005) dapkc⁰⁶⁴⁰³ (Ruiz-Canada et al., 2004) DaPKC^k06403 (Abdelilah-Seyfried et al., 2003, Cox et al., 2001, Wodarz et al., 2000, Zeng et al., 2010) daPKC^k06403 (Zarnescu et al., 2005) l(2)k06403 (Ruiz-Canada et al., 2004, Spradling et al., 1999) l(2)k06403^k06403
Name Synonym
Secondary FlyBase IDs

References ( 38 )

Generate a list of	All references relating to this allele ( 38 )

List References by type	Research paper ( 35 ) Personal communication to FlyBase ( 3 )
Recent research papers ( 7 )
	Leibfried et al., 2013, Development 140(2): 362--371 A Cdc42-regulated actin cytoskeleton mediates Drosophila oocyte polarization. [FBrf0220348] Fletcher et al., 2012, Curr. Biol. 22(12): 1116--1122 Positive feedback and mutual antagonism combine to polarize crumbs in the Drosophila follicle cell epithelium. [FBrf0218646] Guilgur et al., 2012, Development 139(3): 503--513 Drosophila aPKC is required for mitotic spindle orientation during symmetric division of epithelial cells. [FBrf0217157] Haenfler et al., 2012, Dev. Biol. 365(1): 219--228 Cortical aPKC kinase activity distinguishes neural stem cells from progenitor cells by ensuring asymmetric segregation of Numb. [FBrf0218950] Weber et al., 2012, Genetics 191(1): 145--162 Novel regulators of planar cell polarity: a genetic analysis in Drosophila. [FBrf0218210] Jones and Metzstein, 2011, Genetics 189(1): 153--164 A Novel Function for the PAR Complex in Subcellular Morphogenesis of Tracheal Terminal Cells in Drosophila melanogaster. [FBrf0215238] Kaplan et al., 2011, PLoS ONE 6(4): e18616 Complex Interactions between GSK3 and aPKC in Drosophila Embryonic Epithelial Morphogenesis. [FBrf0213424] Show all research papers ...

Allele Dmel\aPKCk06403

Allele Dmel\aPKC^k06403