Allele Dmel\jeb^k05644

General Information
Symbol	Dmel\jeb^k05644	Species	D. melanogaster
Name		FlyBase ID	FBal0064469
Feature type	allele	Associated gene	Dmel\jeb

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (BDGP Project Members, 1994-1999)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 2R:7,998,884..7,998,884 (BDGP Project Members, 1994-1999, Vining et al., 2005, Weiss et al., 2001, Stute et al., 2004, Englund et al., 2003, Spradling et al., 1999, Rohrbough and Broadie, 2010)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{lacW} insertion in an intron. (Weiss et al., 2001)
Caused by insertion	P{lacW}jeb^k05644 (BDGP Project Members, 1994-1999, Vining et al., 2005, Weiss et al., 2001, Stute et al., 2004, Englund et al., 2003, Spradling et al., 1999, Rohrbough and Broadie, 2010)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Spradling et al., 1999, Weiss et al., 2001, BDGP Project Members, 1994-1999) locomotor rhythm defective (Rohrbough and Broadie, 2010) neurophysiology defective (Rohrbough and Broadie, 2010)
Phenotype Manifest In
	circular visceral muscle primordium (Vining et al., 2005) embryonic/larval digestive system (Englund et al., 2003) endoderm (Weiss et al., 2001) muscle founder cell & visceral muscle primordium (Englund et al., 2003) neuromuscular junction (Rohrbough and Broadie, 2010) presumptive embryonic salivary gland (Vining et al., 2005) visceral mesoderm (Weiss et al., 2001, Englund et al., 2003)
Detailed Description
	Statement Reference The central nervous system of jeb[k05644] mutants appears morphologically normal in terms of segmental nerve branching and segmental muscle patterning. Neuropil synaptic differentiation appears normal in these mutants, with comparable labeling intensity, density and distribution of presynaptic and postsynaptic labels. jeb[k05644] mutant neuromuscular junctions appear correctly and stereotypically formed and elaborated, with no examples of muscle innervation failure or synaptic targeting errors. mutant presynaptic active zones appear normally formed and with wild-type size. Homozygous jeb[k05644] mutants exhibit reduced hatching and mutant larval movement is typically sluggish and highly limited. Locomotory movement in jeb[k05644] mutants is decreased to 20% of the control level, characterised by slower contractions and extended pauses. Severely impaired and motionless larvae remain capable of briefly resuming movement when stimulated, suggesting a defect in the central circuit output driving locomotion. jeb[k05644] mutant neuromuscular junctions exhibit visible glutamate-driven muscle contractions, and consistently large and robust postsynaptic current amplitudes compared to controls. jeb[k05644] mutant neuromuscular junctions display excitatory junction currents that are comparable to controls, with amplitudes of greater than 1nA. In jeb[k05644] mutants, no large (exceeding 500pA) or patterned EJCs are recorded. EJCs are absent in 60% of recordings, and overall EJC frequency is below 1Hz in 40% of active cells. (Rohrbough and Broadie, 2010) jeb^k05644 mutants show a salivary gland phenotype. At stage 14, mutant salivary glands remain associated with the inner circular muscle layer, while in wild type, these structures become separate. After stage 15, cells from the distal tips of the jeb^k05644 salivary glands spread into the region of the undifferentiated midgut that forms the gastric caecae in the wild-type embryos. The mutant glands become mispositioned and/or elongated and maintain contact with the area of the midgut immediately adjacent to the proventriculus. (Vining et al., 2005) jeb^k05644 homozygous larvae do not ingest food, and lack discernible intestinal structures. In stage 13 jeb^k05644 homozygous embryos, visceral mesoderm cells are scattered rather than forming an organized band of cells as they would normally do at this stage. At stage 11, the earliest stage at which a mutant phenotype in the visceral mesoderm can clearly be observed, these embryos lack muscle founder cells. However, myoblasts do form in the visceral mesoderm, and go on to contribute to somatic muscle (i.e.- they are fusion competent.) (Englund et al., 2003) Differentiated visceral mesoderm is not seen in mutant embryos, although other mesodermal tissues (somatic muscles, heart, fat body and hemocytes) develop normally. Visceral mesoderm precursor cells are specified but fail to migrate normally. There is an increase in the number of nuclei in positions consistent with an increase in somatic muscle precursors, but there is no major disruption of somatic muscle patterning. The midgut endoderm is specified normally and migrates to form two longitudinal bands. Subsequent dorsal and ventral endoderm migration is abnormal (probably because this migration depends on the visceral mesoderm). (Weiss et al., 2001)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	jeb^k13306a (BDGP Project Members, 1994-1999) jeb^SH0422 (Bazigou et al., 2007)
Rescued by	jeb^k05644 is rescued by jeb^Scer\UAS.cRa/Scer\GAL4^elav-C155 (Rohrbough and Broadie, 2010)
Not rescued by	jeb^k05644 is not rescued by jeb^Scer\UAS.cRa/Scer\GAL4^how-24B/Scer\GAL4^how-24B (Rohrbough and Broadie, 2010)
Comments
Stocks ( 2 )
Bloomington	10576 w^67c23; P{lacW}jeb^k05644/CyO, P{sevRas1.V12}FK1
Kyoto	111183 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}jeb^k05644 /CyO y⁺
Notes on Origin
Discoverer	I. Kiss.

Comments
	Complements: SmD3⁰²⁸³³. Complements: l(2)k13312^k13312. Complements: Nipped-B^k13422a. (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	10576 (Englund et al., 2003) jeb⁵⁷⁶ (Vining et al., 2005) jeb^k05644 (Christensen and Cook, 2006.8.30, Bazigou et al., 2007, Christensen et al., 2008.12.28, Cook and Cook, 2010.5.7, Rohrbough and Broadie, 2010) l(2)k05644 (Spradling et al., 1999) l(2)k05644^k05644 (BDGP Project Members, 1994-1999, ) unnamed (Weiss et al., 2001)
Name Synonym
Secondary FlyBase IDs

References ( 13 )
Research paper	Rohrbough and Broadie, 2010, Development 137(20): 3523--3533 Anterograde Jelly belly ligand to Alk receptor signaling at developing synapses is regulated by Mind the gap. [FBrf0211915] Vining et al., 2005, Dev. Biol. 287(1): 19--34 Organ positioning in Drosophila requires complex tissue-tissue interactions. [FBrf0190174] Stute et al., 2004, Development 131(4): 743--754 Myoblast determination in the somatic and visceral mesoderm depends on Notch signalling as well as on milliways (mili[Alk]) as receptor for Jeb signalling. [FBrf0174588] Englund et al., 2003, Nature 425(6957): 512--516 Jeb signals through the Alk receptor tyrosine kinase to drive visceral muscle fusion. [FBrf0167923] Weiss et al., 2001, Cell 107(3): 387--398 Jelly belly: a Drosophila LDL receptor repeat-containing signal required for mesoderm migration and differentiation. [FBrf0141412] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Supplementary material	Bazigou et al., 2007, Cell 128(5): Supplemental Data. Anterograde Jelly belly and Alk receptor tyrosine kinase signaling mediates retinal axon targeting in Drosophila. [FBrf0199314]
Personal communication to FlyBase	Cook and Cook, 2010.5.7, Isolation and characterization of Df(2R)BSC879. Isolation and characterization of Df(2R)BSC879. [FBrf0210859] Christensen et al., 2008.12.28, Isolation and characterization of Df(2R)BSC699. Isolation and characterization of Df(2R)BSC699. [FBrf0206446] Christensen and Cook, 2006.8.30, Isolation and characterization of Df(2R)BSC199. Isolation and characterization of Df(2R)BSC199. [FBrf0199279] Deal-Herr and Cook, 2003.8.29, Isolation and Characterization of Df(2R)BSC40. Isolation and Characterization of Df(2R)BSC40. [FBrf0161603] Beaton, 1999.12.12, Alleles of the lines in the P-element paper. Alleles of the lines in the P-element paper. [FBrf0125032] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]

Allele Dmel\jebk05644

Allele Dmel\jeb^k05644