Allele Dmel\ab^k02807

General Information
Symbol	Dmel\ab^k02807	Species	D. melanogaster
Name		FlyBase ID	FBal0064575
Feature type	allele	Associated gene	Dmel\ab

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (BDGP Project Members, 1994-1999)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 2L:11,221,528..11,221,528 (BDGP Project Members, 1994-1999, Sugimura et al., 2004, Li et al., 2004, Tan et al., 2012)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	P{lacW}ab^k02807 (BDGP Project Members, 1994-1999, Sugimura et al., 2004, Li et al., 2004, Tan et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| first instar larval stage (with ab^clu-1) (Sugimura et al., 2004) lethal \| recessive (BDGP Project Members, 1994-1999) neuroanatomy defective (Parrish et al., 2006) neuroanatomy defective (with ab^clu-1) (Sugimura et al., 2004)
Phenotype Manifest In
	abdominal posterior ventral multidendritic neuron vdap (Sugimura et al., 2004, Li et al., 2004) abdominal posterior ventral multidendritic neuron vdap (with ab^clu-1) (Sugimura et al., 2004) autophagic vacuole & larval fat body \| somatic clone \| cell autonomous (Arsham and Neufeld, 2009) dorsal multidendritic neuron ddaD (Parrish et al., 2006) dorsal multidendritic neuron ddaD \| somatic clone (Sugimura et al., 2004) dorsal multidendritic neuron ddaE (Parrish et al., 2006) dorsal multidendritic neuron ddaE (with ab^clu-1) (Sugimura et al., 2004) dorsal multidendritic neuron ddaE \| somatic clone (Sugimura et al., 2004) lysosome & larval fat body \| somatic clone \| cell autonomous (Arsham and Neufeld, 2009) mitochondrion & larval fat body \| somatic clone \| cell autonomous (Arsham and Neufeld, 2009)
Detailed Description
	Statement Reference ab[k02807] clones in fat bodies from well-fed early third instar larvae accumulate lysosomes, as indicated by elevated LysoTracker staining, and show redistribution of the Atg8a product into autophagosomes. Mitochondria in ab[k02807] cells aggregate in large clumps. (Arsham and Neufeld, 2009) Targeting of dendrites to antennal lobe glomeruli occurs normally in antennal lobe projection neurons that are part of ab[k02807] homozygous somatic clones. (Komiyama and Luo, 2007) ab^k02807 mutants show a significant reduction in the number of class I neurons, an effect that is most pronounced in the dorsal cluster of PNS neurons. (Parrish et al., 2006) The vpda neurons of ab^k02807/+ larvae show an increased number of dendritic ends. (Li et al., 2004) ab^clu-1/ab^k02807 transheterozygotes hatch and grow normally until 30 hours AEL. The ddaE neurons of ab^clu-1/ab^k02807 larvae do not display such a comb-like design of dendritic arbors as wild-type ddaE neurons. Instead, mutant neurons produce supernumerary terminals that do not necessarily show oriented growth. About 20% of mutant ddaE cells do not extend lateral primary branches and in extreme cases, a single mutant ddaE extends two primary branches dorsally instead. Time-lapse recording of mutant ddaE shows that secondary branches appear with curved morphology and remain associated with fine higher-order branches that are oriented in dorsal and ventral directions. Ventral class I ventral posterior da (vpda) neurons also show dendritic pattern defects. ddaD and ddaE ab^clu-1 mutant neuron clones in heterozygous mature larvae take on an abnormal shape abd produce branch terminals in excess. The ddaD neuron shows a statistically significant increase in the dendritic arbor area size while the area size of ddaE neurons is not significantly different. Class II, III and IV neuron clones do not show dendritic pattern defects. (Sugimura et al., 2004)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	ab²⁶ (Li et al., 2004)
Rescued by	ab^k02807/ab^clu-1 is rescued by ab^{Scer\UAS.S.cSa}/Scer\GAL4^IG1-1 (Sugimura et al., 2004) ab^k02807/ab^clu-1 is rescued by Scer\GAL4^IG1-1/ab^{Scer\UAS.L.cSa} (Sugimura et al., 2004)
Comments
Stocks ( 3 )
Bloomington	10520 y¹ w^*; P{lacW}ab^k02807 P{neoFRT}40A/CyO
Kyoto	102241 y¹ w^67c23 ; P{lacW}ab^k02807 / CyO 111153 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}ab^k02807 P{neoFRT}40A/CyO y⁺
Notes on Origin
Discoverer	I. Kiss.

Comments
	Complements: l(2)k00224^k00224. Complements: cana^k07716. Complements: RfC38^k13807. (BDGP Project Members, 1994-1999) This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	ab^k02807 (Parrish et al., 2006, Kim et al., 2006, Sugimura et al., 2004, Arsham and Neufeld, 2009, Long et al., 2009, Komiyama and Luo, 2007, Tan et al., 2012) ab^K02807 (Jang et al., 2009) ab^l(2)K02807 (Li et al., 2004)
Name Synonym
Secondary FlyBase IDs

References ( 12 )
Research paper	Tan et al., 2012, PLoS Genet. 8(5): e1002681 MicroRNA-277 Modulates the Neurodegeneration Caused by Fragile X Premutation rCGG Repeats. [FBrf0218227] Arsham and Neufeld, 2009, PLoS ONE 4(6): e6068 A genetic screen in Drosophila reveals novel cytoprotective functions of the autophagy-lysosome pathway. [FBrf0208155] Jang et al., 2009, Nat. Cell Biol. 11(5): 569--579 Border-cell migration requires integration of spatial and temporal signals by the BTB protein Abrupt. [FBrf0207765] Long et al., 2009, Development 136(20): 3475--3484 Dendrite branching and self-avoidance are controlled by Turtle, a conserved IgSF protein in Drosophila. [FBrf0208798] Komiyama and Luo, 2007, Curr. Biol. 17(3): 278--285 Intrinsic control of precise dendritic targeting by an ensemble of transcription factors. [FBrf0195086] Kim et al., 2006, Genes Dev. 20(20): 2806--2819 The bHLH-PAS protein Spineless is necessary for the diversification of dendrite morphology of Drosophila dendritic arborization neurons. [FBrf0195191] Parrish et al., 2006, Genes Dev. 20(7): 820--835 Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites. [FBrf0190556] Li et al., 2004, Neuron 43(6): 823--834 BTB/POZ-zinc finger protein abrupt suppresses dendritic branching in a neuronal subtype-specific and dosage-dependent manner. [FBrf0180577] Sugimura et al., 2004, Neuron 43(6): 809--822 Development of morphological diversity of dendrites in Drosophila by the BTB-zinc finger protein abrupt. [FBrf0180576] Johannes and Preiss, 2002, Mech. Dev. 115(1-2): 3--14 Wing vein formation in Drosophila melanogaster: hairless is involved in the cross-talk between Notch and EGF signaling pathways. [FBrf0149093]
Personal communication to FlyBase	Philp, 1998.2.3, l(2)k02807 allelic to ab. l(2)k02807 allelic to ab. [FBrf0100811] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]

Allele Dmel\abk02807

Allele Dmel\ab^k02807