Allele Dmel\Mad^k00237

General Information
Symbol	Dmel\Mad^k00237	Species	D. melanogaster
Name		FlyBase ID	FBal0064630
Feature type	allele	Associated gene	Dmel\Mad
Also Known As	Mad^K237, k00237, Mad²³⁷

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (BDGP Project Members, 1994-1999)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 2L:3,159,608..3,159,608 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Dworkin and Gibson, 2006)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	P{lacW}Mad^k00237 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Dworkin and Gibson, 2006)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999, Dworkin and Gibson, 2006, Kaneko et al., 2011) neuroanatomy defective \| third instar larval stage (Merino et al., 2009, Ball et al., 2010) neuroanatomy defective \| third instar larval stage (with Mad¹) (Ball et al., 2010) neurophysiology defective \| third instar larval stage (Merino et al., 2009) visible \| dominant (Dworkin and Gibson, 2006) visible \| heat sensitive (with Mad^apg) (Galindo et al., 2002)
Phenotype Manifest In
	embryonic/larval neuromuscular junction \| third instar larval stage (Ball et al., 2010) embryonic/larval neuromuscular junction \| third instar larval stage (with Mad¹) (Ball et al., 2010) leg \| distal \| heat sensitive (with Mad^apg) (Galindo et al., 2002) leg \| heat sensitive (with Mad^apg) (Galindo et al., 2002) NMJ bouton \| third instar larval stage (Merino et al., 2009, Ball et al., 2010) NMJ bouton \| third instar larval stage (with Mad¹) (Ball et al., 2010) wing (Dworkin and Gibson, 2006)
Detailed Description
	Statement Reference Mad[1]/Mad[k00237] third instar larvae show a reduced number of boutons per muscle surface area in muscle 4 compared to controls. Homozygous Mad[k00237] third instar larvae show a reduced number of boutons per muscle surface area in muscle 4 compared to controls. (Ball et al., 2010) The number of boutons per muscle surface area is reduced at the neuromuscular junction in Mad[k00237] third instar larvae. Homozygous third instar larvae show a severe reduction in excitatory junctional current amplitude and in quantal content at the neuromuscular junction compared to wild type. (Merino et al., 2009) Heterozygotes show a direct effect on the shape of the wing. (Dworkin and Gibson, 2006) Removal of Mad function before 72 hours after egg laying in Mad^apg/Mad^k00237 flies produces truncations of the proximal-distal axis in the legs, whereas removal of Mad function from 84 hours after egg laying until the end of development results in a normal proximal-distal organisation in the leg despite dorsal patterning defects. (Galindo et al., 2002) Mad^k00237/Mad^apg larvae transferred to 29^oC at the equivalent of 48 hours, 72 hours, 84 hours or 96 hours after egg laying develop at the same rate as wild-type siblings. (Galindo et al., 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Mad^k00237 has neuroanatomy defective \| third instar larval stage phenotype, enhanceable by trio^S137203 (Ball et al., 2010)
Suppressed by
Statement Reference Mad^k00237/Mad¹ has neuroanatomy defective \| third instar larval stage phenotype, suppressible \| partially by Scer\GAL4^BG380/trio^Scer\UAS.cBa (Ball et al., 2010)
NOT suppressed by
Statement Reference Mad^k00237/Mad¹ has neuroanatomy defective \| third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^{Scer\UAS.T:Hsap\MYC} (Ball et al., 2010) Mad^k00237/Mad¹ has size defective \| third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^{Scer\UAS.T:Hsap\MYC} (Ball et al., 2010)
Suppressor of
Statement Reference Mad^k00237/Mad¹ is a suppressor of neuroanatomy defective \| third instar larval stage phenotype of Rac1^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^{elav.Switch.PO} (Ball et al., 2010) Mad^k00237/Mad¹ is a suppressor of size defective \| third instar larval stage phenotype of Rac1^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^BG380 (Ball et al., 2010) Mad^k00237 is a suppressor of visible phenotype of os^GMR.PB (Bach et al., 2003)
NOT Suppressor of
Statement Reference Mad^k00237/Mad¹ is a non-suppressor of neuroanatomy defective \| third instar larval stage phenotype of Rac1^V12.Scer\UAS, Scer\GAL4^BG380 (Ball et al., 2010) Mad^k00237/Mad¹ is a non-suppressor of size defective \| third instar larval stage phenotype of Rac1^V12.Scer\UAS, Scer\GAL4^BG380 (Ball et al., 2010)
Other
Statement Reference +/Df(3L)Exel6279, Mad^k00237 has neuroanatomy defective \| dominant \| third instar larval stage phenotype (Merino et al., 2009) Mad^k00237/Mad[+], nmo^P1 has neuroanatomy defective \| dominant \| third instar larval stage phenotype (Merino et al., 2009)
Phenotype Manifest In
NOT Enhanced by
Statement Reference Mad^k00237 has embryonic/larval neuromuscular junction \| third instar larval stage phenotype, non-enhanceable by trio^S137203 (Ball et al., 2010) Mad^k00237 has NMJ bouton \| third instar larval stage phenotype, non-enhanceable by trio^S137203 (Ball et al., 2010)
Suppressed by
Statement Reference Mad^k00237/Mad¹ has embryonic/larval neuromuscular junction \| third instar larval stage phenotype, suppressible \| partially by Scer\GAL4^BG380/trio^Scer\UAS.cBa (Ball et al., 2010) Mad^k00237/Mad¹ has NMJ bouton \| third instar larval stage phenotype, suppressible \| partially by Scer\GAL4^BG380/trio^Scer\UAS.cBa (Ball et al., 2010)
NOT suppressed by
Statement Reference Mad^k00237/Mad¹ has embryonic/larval neuromuscular junction \| third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^{Scer\UAS.T:Hsap\MYC} (Ball et al., 2010) Mad^k00237/Mad¹ has NMJ bouton \| third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^{Scer\UAS.T:Hsap\MYC} (Ball et al., 2010)
Suppressor of
Statement Reference Mad^k00237/Mad¹ is a suppressor of embryonic/larval neuromuscular junction \| third instar larval stage phenotype of Rac1^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^BG380 (Ball et al., 2010) Mad^k00237/Mad¹ is a suppressor of NMJ bouton \| third instar larval stage phenotype of Rac1^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^BG380 (Ball et al., 2010) Mad^k00237 is a suppressor \| maternal effect of embryonic/first instar larval cuticle \| maternal effect phenotype of tor^12D (Li and Li, 2003) Mad^k00237 is a suppressor of eye phenotype of os^GMR.PB (Bach et al., 2003)
NOT Suppressor of
Statement Reference Mad^k00237/Mad¹ is a non-suppressor of embryonic/larval neuromuscular junction \| third instar larval stage phenotype of Rac1^V12.Scer\UAS, Scer\GAL4^BG380 (Ball et al., 2010) Mad^k00237/Mad¹ is a non-suppressor of NMJ bouton \| third instar larval stage phenotype of Rac1^V12.Scer\UAS, Scer\GAL4^BG380 (Ball et al., 2010)
Other
Statement Reference +/Df(3L)Exel6279, Mad^k00237 has NMJ bouton \| third instar larval stage phenotype (Merino et al., 2009) Mad^k00237/Mad[+], nmo^P1 has NMJ bouton \| third instar larval stage phenotype (Merino et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Expression of Mad[1]/Mad[k00237] suppresses the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1[Scer\UAS.T:Hsap\MYC] is expressed in motor neurons under the control of Scer\GAL4[BG380], instead producing a reduction in bouton number similar to the phenotype seen in Mad[1]/Mad[k00237] third instar larvae. Expression of Mad[1]/Mad[k00237] fails to suppress the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1[V12.Scer\UAS] is expressed in motor neurons under the control of Scer\GAL4[BG380]. trio[S137203] does not enhance the reduction in bouton number phenotype seen in Mad[k00237] larval neuromuscular junctions. Expression of trio[Scer\UAS.cBa] in motor neurons under the control of Scer\GAL4[BG380] partially suppresses the reduced bouton number phenotype seen in Mad[1]/Mad[k00237] larval neuromuscular junctions. (Ball et al., 2010) Mad[k00237]/+ ; nmo[P1]/+ and Mad[k00237]/+ ; Df(3L)Exel6279/+ double heterozygous third instar larvae show a significant reduction in the number of boutons per muscle surface area at the neuromuscular junction, in contrast to any of the single heterozygotes. (Merino et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Mad^k00223 (BDGP Project Members, 1994-1999) Mad^k05807a (BDGP Project Members, 1994-1999) Mad^unspecified (Dworkin and Gibson, 2006)
Rescued by	Mad^k00237 is rescued by Scer\GAL4^BG380/Mad^{S25A.Scer\UAS.T:Hsap\MYC} (Merino et al., 2009) Mad^k00237 is rescued by Scer\GAL4^BG380/Mad^{Scer\UAS.T:Hsap\MYC} (Merino et al., 2009) Mad^k00237 is rescued by Scer\GAL4^unspecified/Mad^{Scer\UAS.T:Hsap\MYC} (Merino et al., 2009)
Partially rescued by	Mad^k00237 is partially rescued by Mad^hs.PK (Kaneko et al., 2011) Mad^k00237 is partially rescued by Mad^T312V.hs (Kaneko et al., 2011) Mad^k00237 is partially rescued by Scer\GAL4^unspecified/Mad^{S25A.Scer\UAS.T:Hsap\MYC} (Merino et al., 2009)
Comments	Expression of Mad[hs.PK] using heat shock partially rescues the lethality of Mad[k00237] mutants, with the rescue efficiency being increased with more frequent heat shocks. Expression of Mad[T312V.hs] using heat shock partially rescues the lethality of Mad[k00237] mutants, with the rescue efficiency being increased with more frequent heat shocks. The degree of rescue with Mad[T312V.hs] is more effective than that seen with Mad[hs.PK] under the same conditions. (Kaneko et al., 2011) Neuronal expression of Mad[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[unspecified] rescues the reduction in the number of boutons per muscle surface area that is seen at the neuromuscular junction in Mad[k00237] third instar larvae. Neuronal expression of Mad[S25A.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[unspecified] partially rescues the reduction in the number of boutons per muscle surface area that is seen at the neuromuscular junction in Mad[k00237] third instar larvae. Expression of either Mad[Scer\UAS.T:Hsap\MYC] or Mad[S25A.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[BG380] rescues the electrophysiological defects seen at the neuromuscular junction of Mad[k00237] third instar larvae. (Merino et al., 2009)
Stocks ( 2 )
Bloomington	10474 y¹ w^67c23; P{lacW}Mad^k00237/CyO
Kyoto	111123 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}Mad^k00237 P{neoFRT}40A/CyO y⁺
Notes on Origin
Discoverer	I. Kiss.

Comments
	Complements: toc⁰¹³⁶¹. Complements: l(2)k05816^k05816. (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	k00237 (Dworkin and Gibson, 2006, Dworkin et al., 2011) l(2)k00237 (Spradling et al., 1999) l(2)k00237^k00237 Mad²³⁷ (Merino et al., 2009, Ball et al., 2010) mad^K00237 (Dworkin and Gibson., 2006) Mad^K237 (Galindo et al., 2002, Galindo et al., 2002) Mad^k00237 (Ball et al., 2010) mad^k00237 (Kaneko et al., 2011) Mad^K00237 (Merino et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 15 )
Research paper	Dworkin et al., 2011, Genetics 187(4): 1171--1184 The effects of weak genetic perturbations on the transcriptome of the wing imaginal disc and its association with wing shape in Drosophila melanogaster. [FBrf0214376] Kaneko et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(27): 11127--11132 Smad inhibition by the Ste20 kinase Misshapen. [FBrf0214278] Ball et al., 2010, Neuron 66(4): 536--549 Retrograde BMP Signaling Controls Synaptic Growth at the NMJ by Regulating Trio Expression in Motor Neurons. [FBrf0210920] Merino et al., 2009, J. Cell Biol. 185(4): 713--725 Nemo kinase interacts with Mad to coordinate synaptic growth at the Drosophila neuromuscular junction. [FBrf0208030] Dworkin and Gibson, 2006, Genetics 173(3): 1417--1431 Epidermal growth factor receptor and transforming growth factor-beta signaling contributes to variation for wing shape in Drosophila melanogaster. [FBrf0193945] Li et al., 2005, Genetics 171(4): 1629--1641 A genetic screen for maternal-effect suppressors of decapentaplegic identifies the eukaryotic translation initiation factor 4A in Drosophila. [FBrf0190738] Bach et al., 2003, Genetics 165(3): 1149--1166 A sensitized genetic screen to identify novel regulators and components of the Drosophila janus kinase/signal transducer and activator of transcription pathway. [FBrf0167623] Li and Li, 2003, Genetics 164(1): 247--258 Drosophila gain-of-function mutant RTK Torso triggers ectopic Dpp and STAT signaling. [FBrf0158996] Galindo et al., 2002, Science 297(5579): 256--259 Leg patterning driven by proximal-distal interactions and EGFR signaling. [FBrf0151488] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Supplementary material	Dworkin and Gibson., 2006, Genetics 173(3): Supplemental Figures. [FBrf0199151] Galindo et al., 2002, Science 296(5579): Supplementary material: leg patterning driven by proximal-distal interactions and EGFR signaling. [FBrf0157204]
Personal communication to FlyBase	Deal and Cook, 2003.1.30, Isolation and characterization of Df(2L)BSC28. Isolation and characterization of Df(2L)BSC28. [FBrf0155398] Beaton, 1999.12.12, Alleles of the lines in the P-element paper. Alleles of the lines in the P-element paper. [FBrf0125032] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]

Allele Dmel\Madk00237

Allele Dmel\Mad^k00237