Allele Dmel\tutl^k14703

General Information
Symbol	Dmel\tutl^k14703	Species	D. melanogaster
Name		FlyBase ID	FBal0064789
Feature type	allele	Associated gene	Dmel\tutl
Also Known As	l(2)k14703

Allele class	hypomorphic allele - genetic evidence
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Bodily et al., 2001)
Mutagen	P-element activity (BDGP Project Members, 1994-1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{lacW} insertion in the region between the 3' border of exon 8 and the 5' border of exon 11. (Bodily et al., 2001)
Caused by insertion	P{lacW}tutl^k14703 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Dean, 2003.6.5, Bodily et al., 2001, Al-Anzi and Wyman, 2009, Ferguson et al., 2009, Vicente-Crespo et al., 2008, Mathew et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective \| adult stage \| recessive (Bodily et al., 2001) behavior defective \| larval stage \| recessive (Bodily et al., 2001) flightless \| recessive (Bodily et al., 2001) lethal \| recessive (Spradling et al., 1999, BDGP Project Members, 1994-1999) neuroanatomy defective (Al-Anzi and Wyman, 2009) neuroanatomy defective (with tutl²³) (Ferguson et al., 2009) partially lethal - majority die \| pupal stage (Al-Anzi and Wyman, 2009) partially lethal - majority live (Bodily et al., 2001)
Phenotype Manifest In
	intersegmental nerve branch ISNb of A1-7 (Al-Anzi and Wyman, 2009) intersegmental nerve branch ISNd of A1-7 (Al-Anzi and Wyman, 2009) optic chiasm (Al-Anzi and Wyman, 2009) photoreceptor cell R7 (with tutl²³) (Ferguson et al., 2009)
Detailed Description
	Statement Reference In tutl[k14703] embryos, the ISNb motor axons succesfully reach the vicinity of their respective targets. However, once there, many fail to send one or more of the final axon branches to contact their muscle targets. Around a quarter of the hemisegments also lack ISNd nerves. Approximately 20% of tutl[k14703] homozygotes reach adulthood. They exhibit an optic chiasm that is disorganised and exhibits graps in the R7 termination line. Flies with eyes composed of tutl[k14703] mutant cells and brains expressing wild-type levels of tutl (generated using the EGUF technique) exhibit smaller eyes than wild-type, although the optic chiasma and R7 projections appear normal, indicating that the source of retinal axon defects in tutl[k14703] mutants is reduced tutl levels in the optic lobe, rather than in the retinal axons. (Al-Anzi and Wyman, 2009) tutl[k14703]/tutl[23] mutants display a mild R7 tiling phenotype. (Ferguson et al., 2009) Homozygous tutl[k14703] embryos show no gastrulation defects. (Mathew et al., 2009) Most homozygotes are able to survive to adulthood if competition with heterozygous siblings is eliminated. However, competition from heterozygous siblings results in the death of homozygous animals (85-100% lethality) before adulthood. General morphology of the mutant larvae is normal. Homozygous larvae have a severely compromised ability to roll over from an inverted position; the time required to right themselves is significantly longer than control larvae. Homozygous adults cannot fly, although they walk normally and energetically on a level surface. The jump response is intact and robust, but attempts at flight result in the flies flipping themselves onto their backs, where continued efforts to fly are shown by the adults flapping their wings at high speeds (this only results in the flies spinning frantically in circles). Adults that become inverted are rarely able to right themselves, despite what appears to be an intense effort to roll over. Only 12-36% of adults are able to roll over after 60 seconds, in contrast to wild-type which roll over immediately, almost always in less than one second. (Bodily et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference tutl^k14703/tutl[+] is a suppressor of lethal \| embryonic stage \| partially \| recessive phenotype of peb³⁰⁸ (Wilk et al., 2004) tutl^k14703/tutl[+] is a suppressor of visible \| heat sensitive phenotype of peb¹ (Wilk et al., 2004) tutl^k14703 is a suppressor of visible phenotype of H^Scer\UAS.cMa/H^Scer\UAS.cMa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Mueller et al., 2005)
Phenotype Manifest In
Enhancer of
Statement Reference tutl⁰¹⁰⁸⁵/tutl^k14703 is an enhancer of eye phenotype of Scer\GAL4^hs.2sev, mbl^C.Scer\UAS (Vicente-Crespo et al., 2008)
Suppressor of
Statement Reference tutl^k14703/tutl[+] is a suppressor of eye \| heat sensitive phenotype of peb¹ (Wilk et al., 2004) tutl^k14703 is a suppressor of eye phenotype of H^Scer\UAS.cMa/H^Scer\UAS.cMa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Mueller et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference A tutl[k14703] ; tutl[01085] mutant background enhances the moderately rough eye phenotype observed upon expression of mbl[C.Scer\UAS] under the control of Scer\GAL4[hs.2sev]. (Vicente-Crespo et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	tutl⁰¹⁰⁸⁵ (Bodily et al., 2001, BDGP Project Members, 1994-1999)
Rescued by	tutl²³/tutl^k14703 is rescued by Scer\GAL4^GMR.long/tutl^Scer\UAS.cFa (Ferguson et al., 2009)
Comments	Expression of either tutl[GH15753.Scer\UAS] or tutl[AT02763.Scer\UAS] driven by the pan-neuronal driver Scer\GAL4[elav-C155] rescues most aspects of tutl[k14703]/tutl[ex383] mutant eye defects. (Al-Anzi and Wyman, 2009) Expression of tutl[Scer\UAS.cFa] under the control of Scer\GAL4[GMR.long] rescues the mild tiling phenotype found in tutl[k14703]/tutl[23] R7 terminals. (Ferguson et al., 2009)
Stocks ( 2 )
Bloomington	10451 y¹ w^67c23; P{lacW}tutl^k14703/CyO
Kyoto	111106 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}tutl^k14703 P{neoFRT}40A/CyO y⁺
Notes on Origin
Discoverer	I. Kiss.

Comments
	Precise excision of the P{lacW} element rescues the semi-lethality and all the behavioural phenotypes of tutl^k14703. (Bodily et al., 2001)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	l(2)01085^k14703 (BDGP Project Members, 1994-1999, ) l(2)k14703 (Bodily et al., 2001, Spradling et al., 1999) P451 (Dean, 2003.6.5) tutl^k14703 (Vicente-Crespo et al., 2008) tutl^K14703 (Ferguson et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 11 )
Research paper	Al-Anzi and Wyman, 2009, Neural Dev. 4: 31 The Drosophila immunoglobulin gene turtle encodes guidance molecules involved in axon pathfinding. [FBrf0209104] Ferguson et al., 2009, J. Neurosci. 29(45): 14151--14159 The conserved Ig superfamily member turtle mediates axonal tiling in Drosophila. [FBrf0209317] Mathew et al., 2009, PLoS ONE 4(10): e7437 A small genomic region containing several loci required for gastrulation in Drosophila. [FBrf0208913] Vicente-Crespo et al., 2008, PLoS ONE 3(2): e1613 Drosophila muscleblind is involved in troponin T alternative splicing and apoptosis. [FBrf0210274] Mueller et al., 2005, Genetics 171(3): 1137--1152 Genetic modifier screens on hairless gain-of function phenotypes reveal genes involved in cell differentiation, cell growth and apoptosis in Drosophila melanogaster. [FBrf0190751] Wilk et al., 2004, Genetics 168(1): 281--300 Dose-sensitive autosomal modifiers identify candidate genes for tissue autonomous and tissue nonautonomous regulation by the Drosophila nuclear zinc-finger protein, hindsight. [FBrf0180294] Bodily et al., 2001, J. Neurosci. 21(9): 3113--3125 A novel member of the Ig superfamily, turtle, is a CNS-specific protein required for coordinated motor control. [FBrf0135975] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Personal communication to FlyBase	Dean, 2003.6.5, Noncomplementation data. Noncomplementation data. [FBrf0159868] Beaton, 1999.12.12, Alleles of the lines in the P-element paper. Alleles of the lines in the P-element paper. [FBrf0125032] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]

Allele Dmel\tutlk14703

Allele Dmel\tutl^k14703