|Name||Saccharomyces cerevisiae UAS construct a of Sigrist||FlyBase ID||FBal0064876|
|Feature type||allele||Associated gene||Dmel\rap|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
Expression of rap[Scer\UAS.cSa] under the control of Scer\GAL4[repo.PU] or Scer\GAL4[c527] results in a dramatic reduction in glial cell number in the eye disc. Expression of rap[Scer\UAS.cSa] under the control of Scer\GAL4[Mz97] has no effect on glial cells in the eye disc.
Expression of rapScer\UAS.cSa under the control of Scer\GAL4prd.RG1 causes inhibition of mitosis 16 in the rapScer\UAS.cSa-expressing embryonic segments.
Expression of rapScer\UAS.cSa in the developing eye, under the control of Scer\GAL4ey.PH, causes three distinct phenotypes: 1. Inhibition of compound eye development. The severity of this ranges from a drastic reduction of the eye to a complete loss of the eye. 2. Induction of ectopic antennae. These antennae can appear fully-differentiated, or may consist of only the first antennal segment. They can occur at the eye position or surrounding regions of the head. Staining of the eye-antennal disc with antennal-specific markers suggests that this ectopic formation is due to an increase in size of the antennal primordia. 3. Induction of abnormally large cells in the eye and more medial regions of the head that form tumour-like structures.
In somatic clones of rapScer\UAS.cSa; Scer\GAL4Act5C.PP cells in the ovarian follicle cells, mitosis arrests (indicated by loss of expression of some mitotic markers) and nuclei become enlarged. FACS profiles of DNA content in follicle cell populations containing these somatic clones show a 32n population not present in wild-type. However, amplification at specific loci (seen after stage 10B in wild-type) occurs normally in these enlarge nuclei.
When rapScer\UAS.cSa is driven by Scer\GAL4twi.PG, most hemisegments of mutant embryos show only one eve expressing pericardial cell (EPC), suggesting that the last division generating two EPCs did not occur. Only four (instead of the normal six) myocardial cells form per hemisegment.
In rapScer\UAS.cSa; Scer\GAL4prd.RG1 embryos, cells in alternate segments fail to execute mitotic cycle 16, resulting in fewer cells per segment in embryos older than stage 12.
Mitosis 16 is blocked in embryos expressing rapScer\UAS.cSa under the control of Scer\GAL4prd.RG1. Inhibits mitotic divisions during imaginal disc cell proliferation when expressed in the posterior compartment of the imaginal discs using Scer\GAL4en-e16E, with the cells undergoing DNA overreplication.
Scer\GAL4prd.RG1, rapScer\UAS.cSa has mitotic cell cycle defective | embryonic stage phenotype, suppressible by CycAScer\UAS.cWa, Scer\GAL4prd.RG1
|Phenotype Manifest In|
Coexpression of CycAScer\UAS.cWa with rapScer\UAS.cSa, under the control of Scer\GAL4prd.RG1, suppresses the inhibition of embryonic mitosis 16 seen when rapScer\UAS.cSa is expressed alone.
Rca1Scer\UAS.T:Ivir\HA1 suppresses the embryonic cycle 16 mitotic block due to a rapScer\UAS.cSa; Scer\GAL4prd.RG1.
Embryos expressing both CycEScer\UAS.cLa and rapScer\UAS.cSa under the control of Scer\GAL4prd.RG1 show normal development until and including mitosis 16 followed by an extra division cycle. Results in an inhibition of mitosis when expressed in CycEAR95 embryos using Scer\GAL4prd.RG1.
|Complementation & Rescue Data|
|Fails to rescue|
Expression of rap[Scer\UAS.cSa] under the control of either Scer\GAL4[gcm.PU] or Scer\GAL4[repo.PU] fails to rescue the increase in glial cell number and glial cell migration defects seen in rap[ie28] embryos. Expression of rap[Scer\UAS.cSa] under the control of Scer\GAL4[elav-C155] does not rescue the increase in glial cell number seen in rap[ie28] embryos, but the glial cell migration defect is completely rescued in 49.6% of hemisegments and is partially rescued in 25.9% of hemisegments. The axonal growth defects seen in the central and peripheral nervous systems are also rescued. Expression of rap[Scer\UAS.cSa] under the control of Scer\GAL4[elav.PLu] almost completely rescues both the glial cell migration defects (71.5% complete rescue, 19% partial rescue) and increase in glial cell number seen in rap[ie28] embryos. The axonal growth defects seen in the central and peripheral nervous systems are also rescued.
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 4 )|
Saccharomyces cerevisiae UAS construct a of Sigrist
|Secondary FlyBase IDs|
|References ( 12 )|