Allele Dmel\rap^Scer\UAS.cSa

General Information
Symbol	Dmel\rap^Scer\UAS.cSa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Sigrist	FlyBase ID	FBal0064876
Feature type	allele	Associated gene	Dmel\rap

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Sigrist and Lehner, 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: rap cDNA is expressed under the control of Scer\UAS regulatory sequences. (Sigrist and Lehner, 1997)
Carried in construct	P{UAS-fzr.S} (Han and Bodmer, 2003, Pimentel and Venkatesh, 2005, Grosskortenhaus and Sprenger, 2002, Araki et al., 2003, Schaeffer et al., 2004, Sigrist and Lehner, 1997, Shcherbata et al., 2004, Reber et al., 2006, Swan and Schupbach, 2007, Narbonne-Reveau et al., 2008, Silies et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| pupal stage, with Scer\GAL4^en-e16E (Swan and Schupbach, 2007) lethal \| pupal stage, with Scer\GAL4^ptc-559.1 (Swan and Schupbach, 2007) mitotic cell cycle defective, with Scer\GAL4^prd.RG1 (Grosskortenhaus and Sprenger, 2002) mitotic cell cycle defective \| embryonic stage, with Scer\GAL4^prd.RG1 (Reber et al., 2006) mitotic cell cycle defective \| somatic clone, with Scer\GAL4^Act5C.PP (Shcherbata et al., 2004) tumorigenic, with Scer\GAL4^ey.PH (Pimentel and Venkatesh, 2005)
Phenotype Manifest In
	adult head, with Scer\GAL4^ey.PH (Pimentel and Venkatesh, 2005) antenna \| ectopic, with Scer\GAL4^ey.PH (Pimentel and Venkatesh, 2005) antennal primordium, with Scer\GAL4^ey.PH (Pimentel and Venkatesh, 2005) cardioblast, with Scer\GAL4^twi.PG (Han and Bodmer, 2003) cycle 16 embryo, with Scer\GAL4^prd.RG1 (Sigrist and Lehner, 1997) embryonic epidermis, with Scer\GAL4^prd.RG1 (Reber et al., 2006) embryonic pericardial cell, with Scer\GAL4^twi.PG (Han and Bodmer, 2003) eye, with Scer\GAL4^ey.PH (Pimentel and Venkatesh, 2005) eye disc, with Scer\GAL4^c527 (Silies et al., 2007) eye disc, with Scer\GAL4^repo.PU (Silies et al., 2007) follicle cell & nucleus \| somatic clone, with Scer\GAL4^Act5C.PP (Shcherbata et al., 2004) glial cell, with Scer\GAL4^c527 (Silies et al., 2007) glial cell, with Scer\GAL4^repo.PU (Silies et al., 2007) imaginal disc & mitotic cell cycle, with Scer\GAL4^en-e16E (Sigrist and Lehner, 1997)
Detailed Description
	Statement Reference Expression of rap[Scer\UAS.cSa] under the control of Scer\GAL4[repo.PU] or Scer\GAL4[c527] results in a dramatic reduction in glial cell number in the eye disc. Expression of rap[Scer\UAS.cSa] under the control of Scer\GAL4[Mz97] has no effect on glial cells in the eye disc. (Silies et al., 2007) Expression of rap^Scer\UAS.cSa under the control of Scer\GAL4^prd.RG1 causes inhibition of mitosis 16 in the rap^Scer\UAS.cSa-expressing embryonic segments. (Reber et al., 2006) Expression of rap^Scer\UAS.cSa in the developing eye, under the control of Scer\GAL4^ey.PH, causes three distinct phenotypes: 1. Inhibition of compound eye development. The severity of this ranges from a drastic reduction of the eye to a complete loss of the eye. 2. Induction of ectopic antennae. These antennae can appear fully-differentiated, or may consist of only the first antennal segment. They can occur at the eye position or surrounding regions of the head. Staining of the eye-antennal disc with antennal-specific markers suggests that this ectopic formation is due to an increase in size of the antennal primordia. 3. Induction of abnormally large cells in the eye and more medial regions of the head that form tumour-like structures. (Pimentel and Venkatesh, 2005) In somatic clones of rap^Scer\UAS.cSa; Scer\GAL4^Act5C.PP cells in the ovarian follicle cells, mitosis arrests (indicated by loss of expression of some mitotic markers) and nuclei become enlarged. FACS profiles of DNA content in follicle cell populations containing these somatic clones show a 32n population not present in wild-type. However, amplification at specific loci (seen after stage 10B in wild-type) occurs normally in these enlarge nuclei. (Shcherbata et al., 2004) When rap^Scer\UAS.cSa is driven by Scer\GAL4^twi.PG, most hemisegments of mutant embryos show only one eve expressing pericardial cell (EPC), suggesting that the last division generating two EPCs did not occur. Only four (instead of the normal six) myocardial cells form per hemisegment. (Han and Bodmer, 2003) In rap^Scer\UAS.cSa; Scer\GAL4^prd.RG1 embryos, cells in alternate segments fail to execute mitotic cycle 16, resulting in fewer cells per segment in embryos older than stage 12. (Grosskortenhaus and Sprenger, 2002) Mitosis 16 is blocked in embryos expressing rap^Scer\UAS.cSa under the control of Scer\GAL4^prd.RG1. Inhibits mitotic divisions during imaginal disc cell proliferation when expressed in the posterior compartment of the imaginal discs using Scer\GAL4^en-e16E, with the cells undergoing DNA overreplication. (Sigrist and Lehner, 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^prd.RG1, rap^Scer\UAS.cSa has mitotic cell cycle defective \| embryonic stage phenotype, suppressible by CycA^Scer\UAS.cWa, Scer\GAL4^prd.RG1 (Reber et al., 2006) Scer\GAL4^prd.RG1, rap^Scer\UAS.cSa has mitotic cell cycle defective phenotype, suppressible by Rca1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^prd.RG1 (Grosskortenhaus and Sprenger, 2002)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^prd.RG1, rap^Scer\UAS.cSa has embryonic epidermis phenotype, suppressible by CycA^Scer\UAS.cWa, Scer\GAL4^prd.RG1 (Reber et al., 2006)
Other
Statement Reference CycE^AR95, Scer\GAL4^prd.RG1, rap^Scer\UAS.cSa has mitotic cell cycle \| embryonic stage phenotype (Sigrist and Lehner, 1997, Sigrist and Lehner, 1997) CycE^Scer\UAS.cLa, Scer\GAL4^prd.RG1, rap^Scer\UAS.cSa has cycle 16 embryo phenotype (Sigrist and Lehner, 1997) CycE^Scer\UAS.cLa, Scer\GAL4^prd.RG1, rap^Scer\UAS.cSa has mitotic cell cycle \| embryonic stage phenotype (Sigrist and Lehner, 1997, Sigrist and Lehner, 1997)
Additional Comments
Genetic Interactions
Statement Reference Coexpression of CycA^Scer\UAS.cWa with rap^Scer\UAS.cSa, under the control of Scer\GAL4^prd.RG1, suppresses the inhibition of embryonic mitosis 16 seen when rap^Scer\UAS.cSa is expressed alone. (Reber et al., 2006) Rca1^{Scer\UAS.T:Ivir\HA1} suppresses the embryonic cycle 16 mitotic block due to a rap^Scer\UAS.cSa; Scer\GAL4^prd.RG1. (Grosskortenhaus and Sprenger, 2002) Embryos expressing both CycE^Scer\UAS.cLa and rap^Scer\UAS.cSa under the control of Scer\GAL4^prd.RG1 show normal development until and including mitosis 16 followed by an extra division cycle. Results in an inhibition of mitosis when expressed in CycE^AR95 embryos using Scer\GAL4^prd.RG1. (Sigrist and Lehner, 1997)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	Scer\GAL4^drl-PGAL8/rap^Scer\UAS.cSa partially rescues rap^x-3 (Pimentel and Venkatesh, 2005) Scer\GAL4^elav-C155/rap^Scer\UAS.cSa partially rescues rap^ie28 (Silies and Klämbt, 2010)
Fails to rescue	Scer\GAL4^gcm.PU/rap^Scer\UAS.cSa fails to rescue rap^ie28 (Silies and Klämbt, 2010) Scer\GAL4^repo.PU/rap^Scer\UAS.cSa fails to rescue rap^ie28 (Silies and Klämbt, 2010)
Comments	Expression of rap[Scer\UAS.cSa] under the control of either Scer\GAL4[gcm.PU] or Scer\GAL4[repo.PU] fails to rescue the increase in glial cell number and glial cell migration defects seen in rap[ie28] embryos. Expression of rap[Scer\UAS.cSa] under the control of Scer\GAL4[elav-C155] does not rescue the increase in glial cell number seen in rap[ie28] embryos, but the glial cell migration defect is completely rescued in 49.6% of hemisegments and is partially rescued in 25.9% of hemisegments. The axonal growth defects seen in the central and peripheral nervous systems are also rescued. Expression of rap[Scer\UAS.cSa] under the control of Scer\GAL4[elav.PLu] almost completely rescues both the glial cell migration defects (71.5% complete rescue, 19% partial rescue) and increase in glial cell number seen in rap[ie28] embryos. The axonal growth defects seen in the central and peripheral nervous systems are also rescued. (Silies and Klämbt, 2010) Expression of rap^Scer\UAS.cSa, under the control of Scer\GAL4^drl-PGAL8, fully rescues the rough eye phenotype in 67% of flies and mediates a partial rescue in the other 33%. (Pimentel and Venkatesh, 2005)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	fzr^Scer\UAS.cSa fzr^UAS.cSa rap^Scer\UAS.cSa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Sigrist (Sigrist and Lehner, 1997)
Secondary FlyBase IDs

References ( 12 )
Research paper	Silies and Klämbt, 2010, Nat. Neurosci. 13(11): 1357--1364 APC/C(Fzr/Cdh1)-dependent regulation of cell adhesion controls glial migration in the Drosophila PNS. [FBrf0212144] Narbonne-Reveau et al., 2008, Development 135(8): 1451--1461 APC/CFzr/Cdh1 promotes cell cycle progression during the Drosophila endocycle. [FBrf0204408] Silies et al., 2007, J. Neurosci. 27(48): 13130--13139 Glial cell migration in the eye disc. [FBrf0201162] Swan and Schupbach, 2007, Development 134(5): 891--899 The Cdc20 (Fzy)/Cdh1-related protein, Cort, cooperates with Fzy in cyclin destruction and anaphase progression in meiosis I and II in Drosophila. [FBrf0194687] Reber et al., 2006, Development 133(16): 3201--3211 Terminal mitoses require negative regulation of Fzr/Cdh1 by Cyclin A, preventing premature degradation of mitotic cyclins and String/Cdc25. [FBrf0195204] Pimentel and Venkatesh, 2005, Dev. Biol. 285(2): 436--446 rap gene encodes Fizzy-related protein (Fzr) and regulates cell proliferation and pattern formation in the developing Drosophila eye-antennal disc. [FBrf0190151] Schaeffer et al., 2004, Curr. Biol. 14(7): 630--636 Notch-dependent Fizzy-related/Hec1/Cdh1 expression is required for the mitotic-to-endocycle transition in Drosophila follicle cells. [FBrf0174489] Shcherbata et al., 2004, Development 131(13): 3169--3181 The mitotic-to-endocycle switch in Drosophila follicle cells is executed by Notch-dependent regulation of G1/S, G2/M and M/G1 cell-cycle transitions. [FBrf0179442] Araki et al., 2003, EMBO J. 22(22): 6115--6126 Degradation of origin recognition complex large subunit by the anaphase-promoting complex in Drosophila. [FBrf0167552] Han and Bodmer, 2003, Development 130(13): 3039--3051 Myogenic cells fates are antagonized by Notch only in asymmetric lineages of the Drosophila heart, with or without cell division. [FBrf0160590] Grosskortenhaus and Sprenger, 2002, Dev. Cell 2(1): 29--40 Rca1 inhibits APC-Cdh1(Fzr) and is required to prevent Cyclin degradation in G2. [FBrf0141469] Sigrist and Lehner, 1997, Cell 90(4): 671--681 Drosophila fizzy-related down-regulates mitotic cyclins and is required for cell proliferation arrest and entry into endocycles. [FBrf0098351]

Allele Dmel\rapScer\UAS.cSa

Allele Dmel\rap^Scer\UAS.cSa