FlyBase Allele Report: Dmel\brat[k06028]

General Information

Symbol

Dmel\brat^k06028

Species

D. melanogaster

Name

FlyBase ID

FBal0064943

Feature type

allele

Associated gene

Dmel\brat

Associated Insertion(s)

P{lacW}brat^k06028

Carried in Construct

Also Known As

l(2)k06028

Genomic Maps

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Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

transposable element insertion site

2L:19,166,314..19,166,314 [+]

(Eroglu et al., 2014, Adamson and Lajeunesse, 2012, Lee et al., 2006, Arama et al., 2000, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

P{lacW} insertion in exon 4 after nucleotide 32500.

(Arama et al., 2000)

Insertion components

P{lacW}brat^k06028

Product class / Tool use(s)

Encoded product / tool

Tagged with

nuclear protein localization tag

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of brain cancer

CEA

(Janic et al., 2010)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates viral infectious disease

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates infectious mononucleosis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates Epstein-Barr virus hepatitis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage (with brat¹¹)

(Xiao et al., 2012)

abnormal neuroanatomy | third instar larval stage

(Liu et al., 2017)

increased cell number | third instar larval stage

(Liu et al., 2017)

lethal

(Landskron et al., 2018)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

neoplasia | third instar larval stage

(Liu et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

brat^k06028 mutants form brain tumors; neuroblasts from these tumors show a severe increase in the frequency of tetraploidy, but not of aneuploidy, polyploidy or mutations, as compared to controls.

(Landskron et al., 2018)

brat^k06028 homozygous third instar larval brains exhibit ganglion mother cell dedifferentiation and severe tumor phenotypes, as compared to controls.

(Liu et al., 2017)

brat¹¹/brat^k06028 larval brains contain supernumerary type II neuroblasts.

(Xiao et al., 2012)

Homozygous larval brain tissue develops into tumours after allograft into adult host flies (taking over the entire abdomen of the host) in 80% of cases.

(Janic et al., 2010)

When pieces of brain from brat^k06028 9-12-day-old larvae, which show tissue overgrowth, are transplanted into the abdomens of adult hosts, tumors of the abdomen develop.

(Caussinus and Gonzalez, 2005)

Embryos laid by brat^k06028/brat^fs3 mothers have 5 to 8 abdominal segments. brat^k06028/brat^k06028 embryos generated by germ-line clone have 6 to 8 abdominal segments.

(Sonoda and Wharton, 2001)

The brain hemispheres of mutant third instar larvae are highly enlarged compared to wild type, while the imaginal discs are not affected.

(Arama et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

brat^k06028 has lethal phenotype, suppressible by lncRNA:cherub^DEL/lncRNA:cherub^DEL

(Landskron et al., 2018)

brat^k06028 has neoplasia phenotype, suppressible by lncRNA:cherub^DEL/lncRNA:cherub^DEL

(Landskron et al., 2018)

brat^k06028/brat¹¹ has abnormal neuroanatomy | larval stage phenotype, suppressible | somatic clone by klu^unspecified/klu^unspecified

(Xiao et al., 2012)

NOT suppressed by

Statement

Reference

brat^k06028 has neoplasia phenotype, non-suppressible by piwi¹

(Janic et al., 2010)

NOT Enhancer of

Statement

Reference

brat[+]/brat^k06028 is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407, mir-ban^{sponge.UAS.DsRed(Unk)}

(Wu et al., 2017)

brat[+]/brat^k06028 is a non-enhancer of increased cell number | larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407, mir-ban^{sponge.UAS.DsRed(Unk)}

(Wu et al., 2017)

NOT Suppressor of

Statement

Reference

brat[+]/brat^k06028 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407, mir-ban^{sponge.UAS.DsRed(Unk)}

(Wu et al., 2017)

brat[+]/brat^k06028 is a non-suppressor of increased cell number | larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407, mir-ban^{sponge.UAS.DsRed(Unk)}

(Wu et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

brat^k06028 has brain phenotype, suppressible by lncRNA:cherub^DEL/lncRNA:cherub^DEL

(Landskron et al., 2018)

brat^k06028/brat¹¹ has type II neuroblast | increased number | larval stage phenotype, suppressible | somatic clone by klu^unspecified/klu^unspecified

(Xiao et al., 2012)

Enhancer of

Statement

Reference

brat^k06028 is an enhancer of eye phenotype of EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

NOT Enhancer of

Statement

Reference

brat[+]/brat^k06028 is a non-enhancer of type II neuroblast | increased number | larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407, mir-ban^{sponge.UAS.DsRed(Unk)}

(Wu et al., 2017)

NOT Suppressor of

Statement

Reference

brat[+]/brat^k06028 is a non-suppressor of type II neuroblast | increased number | larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407, mir-ban^{sponge.UAS.DsRed(Unk)}

(Wu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The presence of brat^k06028/+ does not change the number of neuroblasts per brain lobe seen in larval brains expressing both ban^{sponge.Scer\UAS.T:Disc\RFP} and N^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^insc-Mz1407.

(Wu et al., 2017)

The formation of supernumerary type II neuroblasts is suppressed in homozygous klu^unspecified type II neuroblast clones in brat¹¹/brat^k06028 larval brains.

(Xiao et al., 2012)

The frequency with which brat^k06028 larval brain tissue develops into tumours after allograft into adult host flies (taking over the entire abdomen of the host) is not significantly altered if the transplanted tissue is also homozygous for piwi¹.

(Janic et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

brat^k11538

(BDGP Project Members, 1994-1999)

brat^k11523

(BDGP Project Members, 1994-1999)

brat^k11508

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

114346

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}brat^k06028 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Excision of the P{lacW} element can result in viable, fertile revertants.

(Arama et al., 2000)

Complements: l(2)10206¹⁰²⁰⁶. Complements: l(2)k09613^k09613.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

brat<up> k06028</up>

(Adamson and Lajeunesse, 2012)

brat^K06028

(Reichardt et al., 2018, Arama et al., 2000)

brat^k06028

(Landskron et al., 2018, Loewen et al., 2018, Liu et al., 2017, Rossi et al., 2017, Wu et al., 2017, Eroglu et al., 2014, Janssens et al., 2014, Komori et al., 2014, Xiao et al., 2012, Janic et al., 2010, Caussinus and Gonzalez, 2005)

brat^k

(Wu et al., 2017)

l(2)k06028

(Spradling et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (20)

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