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General Information
Symbol
Dmel\KrUAS.cHa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Hoch
FlyBase ID
FBal0065072
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Kr
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of Kr.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Scer\GAL4Mef2.PR-mediated expression of KrScer\UAS.cHa does not result in muscle defects.

Expression of KrScer\UAS.cHa using Scer\GAL4how-24B leads to mis-arrangements and patterning defects of the somatic musculature, including reduced numbers and altered shapes of the lateral transverse (LT) muscles. The remaining LT muscles are often bent at their ventral endings and others appear thicker and shorter.

Third instar larvae expressing KrScer\UAS.cHa under the control of Scer\GAL4Pxn.PS contain an increased proportion of lamellocytes as a proportion of total hemocyte number (9.5% compared to 0.5% in controls). Hemocytes are unable to undergo phagocytosis of small particles; unlike controls, they do not engulf India ink particles, instead adhering to larger particles India ink particles.

Approximately 40% of flies expressing KrScer\UAS.cHa under the control of Scer\GAL4Pxn.PS exhibit melanotic tumours.

Expression of KrScer\UAS.cHa under the control of Scer\GAL4pros.PMG at 22oC results in each hemisegment having five to six eve+ U neurons, mostly a U1, a U2 and three U3 neurons (91%), but sometimes a U1, a U2 and four U3 neurons (9%). Expression of two copies of KrScer\UAS.cHa under the control of Scer\GAL4pros.PMG at 29oC results in extra eve+ U neurons; each hemisegment contains on average a U1, a U2, 7.8 U3 neurons and no U4/U5 neurons. Embryos expressing KrScer\UAS.cHa under the control of Scer\GAL4sca-4512 have a total number of 7 to 8 eve+ U neurons in each hemisegment, although ectopic U3 neurons range from 2 to 6 in number. Hemisegments with only two ectopic U3 neurons typically have U4/U5 neurons, those with 3 ectopic U3 neurons have only a U4 neuron and those with four or more ectopic U3 neurons lack both U4/U5 neuronal fates.

When KrScer\UAS.cHa is driven by one copy of Scer\GAL4ftz.ng a minor SNb phenotype is seen. The distal RP axons fail to reach their target muscles and maintain growth cone like structures at their ends. With two copies, a stronger phenotype is seen. In 36% of cases, the most distal RP axons stall RP5 axons does not innervate the target muscle 12. Whereas in all other cases, the SNb stalls at the second choice point. When a single copy of KrScer\UAS.cHa is driven by Scer\GAL4how-24B the RP3 axon separates properly from the SNb and finds the cleft between target muscles 7 and 6, whereas the remaining RP axons fail to defasciculate. Two copies of KrScer\UAS.cHa causes stronger defects. The SNb stalls at the position where RP3 would normally defasciculate. KrScer\UAS.cHa driven by Scer\GAL4twi.PG also gives axon guidance phenotypes in the RP axons.

When KrScer\UAS.cHa is driven Scer\GAL4en-e16E, there are extra cells in the neuroblast 7-3 lineage and all but 2 cells differentiate as GMC-2 derived interneuron 2; the two unaffected cells are the GMC-1 derived 1/1G neurons. Extra eve+ neurons are also seen in these animals, with all differentiating as U3 or U4 motorneurons, except the normal pair of early born U1/U2 motorneurons.

When KrScer\UAS.cHa is expressed under the control of Scer\GAL4ptc-559.1 in the embryonic hindgut, extra cells express ct and evert from the hindgut to produce enlarged Malpighian tubule primordia.

Flies expressing KrScer\UAS.cHa under the control of Scer\GAL4hs.2sev show a reduction in the size of the eye and irregular arrangement of the ommatidia. A similar though less severe eye phenotype is produced in flies expressing KrScer\UAS.cHa under the control of Scer\GAL4ey.PH.

Embryos expressing KrScer\UAS.cHa under the control of Scer\GAL4da.G32 develop multiple Malpighian tubule tip cells. The satellite cell is transformed into a second Malpighian tubule tip cell in embryos expressing KrScer\UAS.cHa under the control of Scer\GAL4ase.PS.

When expressed via Scer\GAL4how-24B, Scer\GAL4twi.PB, two muscles with the orientation and insertion sites of VA2 are formed and VA1 is missing.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Scer\GAL4Mef2.PR-mediated expression of KrScer\UAS.cHa in a Sin3A08269/+ background results in 55% of embryos reproducibly displaying a muscle transformation of VA1 to VA2.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Pan-muscular expression of Kr can partially rescue the SNb phenotype of the Kr1, KrmCD, caps05121 double homozygotes.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

M. Hoch and A. Michelson.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
KrScer\UAS.cHa
KrUAS.cHa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Hoch
Secondary FlyBase IDs
    References (12)