FlyBase Allele Report: Scer\GAL4[c42]

Drives expression in adult Malpighian tubule structures, including principal cells of the main segment, in bar-shaped cells of the initial and transitional segments, and the lower tubule. In the adult brain, drives expression in the ellipsoid and fan-shaped bodies and pars intercerbralis, in about 13 ring neurons R2 and R4m, and in a subset of fan-shaped neurons.

(FlyBase Curators, 2017)

ScerGAL4^c42 labeling is detected in about 13 ring neurons R2 and R4m at 7-8 days of age.

(Martín-Peña et al., 2014)

ScerGAL4^c42 drives expression in Malphighian tubule type I cells (principal cells).

(Terhzaz et al., 2010)

In adult Malpighian tubule, expressed in principal cells of the main segment, in bar-shaped cells of the initial and transitional segments, and in the lower tubule.

(Rosay et al., 1997)

Marker for

Malpighian tubule Type I cell

(Söderberg et al., 2011)

adult ellipsoid body ring neuron

(Renn et al., 1999)

ellipsoid body

(Renn et al., 1999)

fan-shaped body

(Renn et al., 1999)

fan-shaped body tangential neuron

(Renn et al., 1999)

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | larval stage, with Ggt-1^RNAi.UAS

(Liu et al., 2014)

abnormal body size | larval stage, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

abnormal body size | P-stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

abnormal developmental rate, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

abnormal locomotor behavior, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

abnormal oxidative stress response, with sesB^RNAi.UAS

(Terhzaz et al., 2010)

abnormal stress response, with salt^RNAi.WIZ.UAS

(Stergiopoulos et al., 2009)

decreased cell number, with Dronc^pro.UAS

(Shukla and Tapadia, 2011)

decreased cell number | larval stage, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

lethal, with grim^UAS.cUa

(Shukla and Tapadia, 2011)

lethal, with hid^UAS.cUa

(Shukla and Tapadia, 2011)

lethal - all die before end of first instar larval stage, with Dronc^ΔN.UAS

(Shukla and Tapadia, 2011)

lethal - all die before end of first instar larval stage, with rpr^UAS.C

(Shukla and Tapadia, 2011)

lethal - all die before end of larval stage, with pdm3^UAS.CFP

(Chen et al., 2012)

lethal - all die during first instar larval stage, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

lethal - all die during P-stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

partially lethal - majority die, with Dronc^pro.UAS

(Shukla and Tapadia, 2011)

partially lethal - majority die, with Myc^UAS.cZa

(Gamberi et al., 2017)

some die during first instar larval stage, with Dronc^ΔN.UAS

(Shukla and Tapadia, 2011)

some die during first instar larval stage, with rpr^UAS.C

(Shukla and Tapadia, 2011)

some die during larval stage, with pdm3^UAS.CFP

(Chen et al., 2012)

visible, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

Phenotype Manifest In

adult abdomen | P-stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

adult head | P-stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

adult main segment of Malpighian tubule | pharate adult stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

adult Malpighian tubule, with fray^RNAi.UAS

(Wu et al., 2014)

adult Malpighian tubule, with Myc^UAS.cZa

(Gamberi et al., 2017)

adult Malpighian tubule, with Nha1^UAS.EYFP

(Day et al., 2008)

adult Malpighian tubule, with Wnk^{D420A.UAS.cWa}

(Wu et al., 2014)

adult Malpighian tubule, with Wnk^KK102654

(Wu et al., 2014)

adult Malpighian tubule principal cell | pharate adult stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

adult thorax | P-stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

embryonic/first instar larval cuticle, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

embryonic/larval Malpighian tubule, with Dronc^pro.UAS

(Shukla and Tapadia, 2011)

embryonic/larval Malpighian tubule, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

embryonic/larval Malpighian tubule, with sesB^RNAi.UAS

(Terhzaz et al., 2010)

embryonic/larval Malpighian tubule | third instar larval stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

embryonic/larval Malpighian tubule Type I cell, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

embryonic/larval Malpighian tubule Type I cell | third instar larval stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

embryonic/larval Malpighian tubule Type II cell, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

larval adipose system, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

Malpighian tubule, with 5-HT7^UAS.cKa

(Kerr et al., 2004)

Malpighian tubule, with Besp\bPAC^UAS.cSa

(Efetova et al., 2013)

Malpighian tubule, with Besp\bPAC^UAS.cSa, Scer\GAL4^tsh-c724

(Efetova et al., 2013)

Malpighian tubule, with Btau\PDE5A^UAS.cBa

(Broderick et al., 2004)

Malpighian tubule, with Oatp58Db^RNAi.UAS

(Torrie et al., 2004)

Malpighian tubule, with Pka-C1^VDRC.cUa

(Efetova et al., 2013)

Malpighian tubule, with SPoCk^{A.UAS.Tag:MYC}

(Southall et al., 2006)

Malpighian tubule, with SPoCk^RNAi.UAS.WIZ

(Southall et al., 2006)

Malpighian tubule | conditional, with Besp\bPAC^UAS.cSa

(Efetova et al., 2013)

Malpighian tubule | drug conditional, with Rnor\Npr1^UAS.cKa

(Kerr et al., 2004)

Malpighian tubule | P-stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

mitochondrion | larval stage, with sesB^RNAi.UAS

(Terhzaz et al., 2010)

nucleus | pharate adult stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

nucleus | third instar larval stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

pupal tracheal section, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

puparium cuticle, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Besp\bPAC^UAS.cSa, Scer\GAL4^c42 has Malpighian tubule | conditional phenotype, enhanceable by Epac^UAS.cEa, Scer\GAL4^c42

(Efetova et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^c42, Wnk^KK102654 has adult Malpighian tubule phenotype, suppressible by Scer\GAL4^c42/fray^T206E.UAS

(Wu et al., 2014)

Scer\GAL4^c42, Wnk^KK102654 has adult Malpighian tubule phenotype, suppressible by Ncc69^r2

(Wu et al., 2014)

Scer\GAL4^c42, Wnk^KK102654 has adult Malpighian tubule phenotype, suppressible by Scer\GAL4^c42/fray^RNAi.UAS

(Wu et al., 2014)

Enhancer of

Statement

Reference

Epac^UAS.cEa, Scer\GAL4^c42 is an enhancer of Malpighian tubule | conditional phenotype of Besp\bPAC^UAS.cSa, Scer\GAL4^c42

(Efetova et al., 2013)

Suppressor of

Statement

Reference

Myc^JF01761, Scer\GAL4^c42, Scer\GAL4^tsh-c724 is a suppressor of adult Malpighian tubule phenotype of BicC⁵

(Gamberi et al., 2017)

Myc^JF01761, Scer\GAL4^c42, Scer\GAL4^tsh-c724 is a suppressor of adult specialized Malpighian tubule cell phenotype of BicC⁵

(Gamberi et al., 2017)

Myc^JF01762, Scer\GAL4^c42, Scer\GAL4^tsh-c724 is a suppressor of adult Malpighian tubule phenotype of BicC⁵

(Gamberi et al., 2017)

Myc^JF01762, Scer\GAL4^c42, Scer\GAL4^tsh-c724 is a suppressor of adult specialized Malpighian tubule cell phenotype of BicC⁵

(Gamberi et al., 2017)

Scer\GAL4^c42/fray^T206E.UAS is a suppressor of adult Malpighian tubule phenotype of Scer\GAL4^c42, Wnk^KK102654

(Wu et al., 2014)

Scer\GAL4^c42/fray^RNAi.UAS is a suppressor of adult Malpighian tubule phenotype of Scer\GAL4^c42, Wnk^KK102654

(Wu et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^c42/L2HGDH^UAS.cLa rescues L2HGDH¹²/L2HGDH¹⁴

(Mahmoudzadeh et al., 2024)

Scer\GAL4^c42/Ggt-1^UAS.cLa rescues Ggt-1^Δ1523

(Liu et al., 2014)

Scer\GAL4^c42/homer^UAS.Tag:MYC rescues homer^R102

(Urizar et al., 2007)

Partially rescues

Scer\GAL4^c42/EcR^A.UAS partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

EcR^B1.UAS/Scer\GAL4^c42 partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

Scer\GAL4^c42/EcR^B2.UAS partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

Fails to rescue

Scer\GAL4^c42/ERR^UAS.cTa fails to rescue ERR²/ERR¹

(Tennessen et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

30835

w^*; P{GawB}c42

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

GAL4^c42

Scer\GAL4^c42

Name Synonyms

Secondary FlyBase IDs

References (63)

Report Sections

Open Close

General Information
Nature of the Allele
Expression Data
Human Disease Associations
Phenotypic Data
Interactions
Complementation and Rescue Data
Images
Stocks
Notes On Origin
Crossreferences
Synonyms and Secondary IDs
References