Allele Scer\GAL4^Ser.PGF

General Information
Symbol	Scer\GAL4^Ser.PGF	Species	S. cerevisiae
Name	Serrate promoter construct of Gu and Fleming	FlyBase ID	FBal0065106
Feature type	allele	Associated gene	Scer\GAL4

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Hukriede et al., 1997, Fleming et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference The regulatory sequences in P{Ser-GAL4.GF} that drive the expression pattern come from the 3' region of Ser and are there in conjunction with the Ser promoter to drive Scer\GAL4. (Fleming, 2003.7) Construct: The Ser promoter drive Scer\GAL4 expression. (Fleming et al., 1997) Construct: One copy of the Ser promoter drives Scer\GAL4 expression. (Hukriede et al., 1997)
Carried in construct	P{Ser-GAL4.GF} (Fleming, 2003.7, Cherbas et al., 2003, Certel et al., 2000, Kim et al., 2006, Le Borgne et al., 2005, Hukriede et al., 1997, Zeng et al., 1998, Fleming et al., 1997, Bardin and Schweisguth, 2006, Turki-Judeh and Courey, 2012, Kim et al., 2012, Datta et al., 2009, Hamel et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with EcR^{B1-ΔC655.F645A.Scer\UAS} (Cherbas et al., 2003) lethal \| pupal stage, with Dl::Ser^NT.Scer\UAS (Fleming et al., 1997) lethal \| pupal stage \| heat sensitive, with tkv^{Q253D.Scer\UAS.cLa} (Kim et al., 2006) visible, with Bx^EP1394 (Zeng et al., 1998) visible, with Egfr^Scer\UAS.cUa (Kim et al., 2012) visible, with gro^{Scer\UAS.P\T.WT} (Turki-Judeh and Courey, 2012) visible, with gro^{ΔCcN.Scer\UAS.P\T} (Turki-Judeh and Courey, 2012) visible, with gro^{ΔCR.Scer\UAS.P\T} (Turki-Judeh and Courey, 2012) visible, with gro^{ΔSP.Scer\UAS.P\T} (Turki-Judeh and Courey, 2012) visible, with mib1^{C1205S.Scer\UAS} (Hamel et al., 2010) visible, with mib1^GD11786 (Hamel et al., 2010) visible, with pum^Scer\UAS.cKa (Kim et al., 2012) visible, with tio^Scer\UAS.cLa (Datta et al., 2009) visible, with tsh^Scer\UAS.cGa (Datta et al., 2009) visible, with vvl^{B157.Scer\UAS} (Certel et al., 2000) visible, with vvl^Scer\UAS.cCa (Certel et al., 2000)
Phenotype Manifest In
	antennal disc \| ectopic \| third instar larval stage, with tio^Scer\UAS.cLa (Datta et al., 2009) antennal disc \| ectopic \| third instar larval stage, with tsh^Scer\UAS.cGa (Datta et al., 2009) eye \| ectopic, with tio^Scer\UAS.cLa (Datta et al., 2009) eye \| ectopic, with tsh^Scer\UAS.cGa (Datta et al., 2009) wing, with Bx^EP1394 (Zeng et al., 1998) wing, with Dl^Scer\UAS.cJa (Hukriede et al., 1997) wing, with gro^{Scer\UAS.P\T.WT} (Turki-Judeh and Courey, 2012) wing, with gro^{ΔCR.Scer\UAS.P\T} (Turki-Judeh and Courey, 2012) wing, with gro^{ΔSP.Scer\UAS.P\T} (Turki-Judeh and Courey, 2012) wing, with mib1^{C1205S.Scer\UAS} (Hamel et al., 2010) wing, with mib1^GD11786 (Hamel et al., 2010) wing, with Ser^{Bd.Scer\UAS.T:Hsap\MYC} (Hukriede et al., 1997) wing, with tkv^{Q253D.Scer\UAS.cLa} (Kim et al., 2006) wing, with Tollo^ex.Scer\UAS (Kim et al., 2006) wing, with Tollo^Scer\UAS.cKa (Kim et al., 2006) wing, with vvl^{B157.Scer\UAS} (Certel et al., 2000) wing \| distal, with vvl^Scer\UAS.cCa (Certel et al., 2000) wing cell \| ectopic, with vvl^Scer\UAS.cCa (Certel et al., 2000) wing disc \| dorsal compartment, with Dl^Scer\UAS.cJa (Fleming et al., 1997, Hukriede et al., 1997) wing disc \| dorsal compartment, with Ser^{Bd.Scer\UAS.T:Hsap\MYC} (Hukriede et al., 1997) wing margin, with mib1^{C1205S.Scer\UAS} (Bardin and Schweisguth, 2006) wing margin, with neur^{C701S.Scer\UAS} (Bardin and Schweisguth, 2006) wing margin, with pum^Scer\UAS.cKa (Kim et al., 2012) wing vein \| ectopic, with Egfr^Scer\UAS.cUa (Kim et al., 2012) wing vein L4, with gro^{ΔCcN.Scer\UAS.P\T} (Turki-Judeh and Courey, 2012) wing vein L5, with gro^{ΔCcN.Scer\UAS.P\T} (Turki-Judeh and Courey, 2012)
Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference Scer\GAL4^Ser.PGF, pum^Scer\UAS.cKa has visible phenotype, non-enhanceable by Egfr^Scer\UAS.cUa, Scer\GAL4^Ser.PGF (Kim et al., 2012, Kim et al., 2012)
Suppressed by
Statement Reference Scer\GAL4^Ser.PGF, mib1^{C1205S.Scer\UAS} has visible phenotype, suppressible by α4GT1^GS2078, Scer\GAL4^Ser.PGF (Hamel et al., 2010, Hamel et al., 2010) Scer\GAL4^Ser.PGF, mib1^{C1205S.Scer\UAS} has visible phenotype, suppressible by α4GT1^Scer\UAS.cHa, Scer\GAL4^Ser.PGF (Hamel et al., 2010, Hamel et al., 2010) Scer\GAL4^Ser.PGF, mib1^{C1205S.Scer\UAS} has visible phenotype, suppressible by α4GT2^Scer\UAS.cHa, Scer\GAL4^Ser.PGF (Hamel et al., 2010, Hamel et al., 2010) Scer\GAL4^Ser.PGF, mib1^GD11786 has visible phenotype, suppressible by α4GT1^GS2078, Scer\GAL4^Ser.PGF (Hamel et al., 2010, Hamel et al., 2010)
NOT suppressed by
Statement Reference Scer\GAL4^Ser.PGF, pum^Scer\UAS.cKa has visible phenotype, non-suppressible by Egfr^Scer\UAS.cUa, Scer\GAL4^Ser.PGF (Kim et al., 2012, Kim et al., 2012)
NOT Enhancer of
Statement Reference Egfr^Scer\UAS.cUa, Scer\GAL4^Ser.PGF is a non-enhancer of visible phenotype of Scer\GAL4^Ser.PGF, pum^Scer\UAS.cKa (Kim et al., 2012, Kim et al., 2012)
Suppressor of
Statement Reference α4GT1^GS2078, Scer\GAL4^Ser.PGF is a suppressor of visible phenotype of Scer\GAL4^Ser.PGF, mib1^{C1205S.Scer\UAS} (Hamel et al., 2010, Hamel et al., 2010) α4GT1^GS2078, Scer\GAL4^Ser.PGF is a suppressor of visible phenotype of Scer\GAL4^Ser.PGF, mib1^GD11786 (Hamel et al., 2010, Hamel et al., 2010) α4GT1^GS2078/Scer\GAL4^Ser.PGF is a suppressor of visible phenotype of mib1⁴/mib1³ (Hamel et al., 2010) α4GT1^Scer\UAS.cHa, Scer\GAL4^Ser.PGF is a suppressor of visible phenotype of Scer\GAL4^Ser.PGF, mib1^{C1205S.Scer\UAS} (Hamel et al., 2010, Hamel et al., 2010) α4GT2^Scer\UAS.cHa, Scer\GAL4^Ser.PGF is a suppressor of visible phenotype of Scer\GAL4^Ser.PGF, mib1^{C1205S.Scer\UAS} (Hamel et al., 2010, Hamel et al., 2010)
NOT Suppressor of
Statement Reference Egfr^Scer\UAS.cUa, Scer\GAL4^Ser.PGF is a non-suppressor of visible phenotype of Scer\GAL4^Ser.PGF, pum^Scer\UAS.cKa (Kim et al., 2012, Kim et al., 2012) α4GT1^GS2078/Scer\GAL4^Ser.PGF is a non-suppressor of visible phenotype of mib1³/Df(3L)mib1² (Hamel et al., 2010)
Phenotype Manifest In
Suppressed by
Statement Reference Egfr^Scer\UAS.cUa, Scer\GAL4^Ser.PGF has wing vein \| ectopic phenotype, suppressible by pum^Scer\UAS.cKa, Scer\GAL4^Ser.PGF (Kim et al., 2012, Kim et al., 2012) Scer\GAL4^Ser.PGF, dpp^Scer\UAS.cFa has eye phenotype, suppressible by Tollo^Scer\UAS.cKa (Kim et al., 2006) Scer\GAL4^Ser.PGF, dpp^Scer\UAS.cFa has wing phenotype, suppressible by Tollo^Scer\UAS.cKa (Kim et al., 2006) Scer\GAL4^Ser.PGF, mib1^{C1205S.Scer\UAS} has wing phenotype, suppressible by α4GT1^GS2078, Scer\GAL4^Ser.PGF (Hamel et al., 2010, Hamel et al., 2010) Scer\GAL4^Ser.PGF, mib1^{C1205S.Scer\UAS} has wing phenotype, suppressible by α4GT1^Scer\UAS.cHa, Scer\GAL4^Ser.PGF (Hamel et al., 2010, Hamel et al., 2010) Scer\GAL4^Ser.PGF, mib1^{C1205S.Scer\UAS} has wing phenotype, suppressible by α4GT2^Scer\UAS.cHa, Scer\GAL4^Ser.PGF (Hamel et al., 2010, Hamel et al., 2010) Scer\GAL4^Ser.PGF, mib1^GD11786 has wing phenotype, suppressible by α4GT1^GS2078, Scer\GAL4^Ser.PGF (Hamel et al., 2010, Hamel et al., 2010) Scer\GAL4^Ser.PGF, neur^{C701S.Scer\UAS} has wing margin phenotype, suppressible by Tom^Scer\UAS.cLa (Bardin and Schweisguth, 2006) Scer\GAL4^Ser.PGF, tkv^{Q253D.Scer\UAS.cLa} has wing phenotype, suppressible by Tollo^Scer\UAS.cKa (Kim et al., 2006) Scer\GAL4^Ser.PGF, Tollo^ex.Scer\UAS has wing phenotype, suppressible by tkv^{Q253D.Scer\UAS.cLa} (Kim et al., 2006) Scer\GAL4^Ser.PGF, Tollo^Scer\UAS.cKa has wing phenotype, suppressible by dpp^Scer\UAS.cFa (Kim et al., 2006) Scer\GAL4^Ser.PGF, Tollo^Scer\UAS.cKa has wing phenotype, suppressible by tkv^{Q253D.Scer\UAS.cLa} (Kim et al., 2006)
NOT suppressed by
Statement Reference Scer\GAL4^Ser.PGF, mib1^{C1205S.Scer\UAS} has wing margin phenotype, non-suppressible by Tom^Scer\UAS.cLa (Bardin and Schweisguth, 2006)
Suppressor of
Statement Reference N^{FLN.ΔCdc10.Scer\UAS}/Scer\GAL4^Ser.PGF is a suppressor of wing disc phenotype of mib1^EY97600/mib1³ (Le Borgne et al., 2005) pum^Scer\UAS.cKa, Scer\GAL4^Ser.PGF is a suppressor of wing vein \| ectopic phenotype of Egfr^Scer\UAS.cUa, Scer\GAL4^Ser.PGF (Kim et al., 2012, Kim et al., 2012) Scer\GAL4^Ser.PGF/neur^Scer\UAS.cPa is a suppressor of wing disc phenotype of mib1^EY97600/mib1³ (Le Borgne et al., 2005) α4GT1^GS2078, Scer\GAL4^Ser.PGF is a suppressor of wing phenotype of Scer\GAL4^Ser.PGF, mib1^{C1205S.Scer\UAS} (Hamel et al., 2010, Hamel et al., 2010) α4GT1^GS2078, Scer\GAL4^Ser.PGF is a suppressor of wing phenotype of Scer\GAL4^Ser.PGF, mib1^GD11786 (Hamel et al., 2010, Hamel et al., 2010) α4GT1^GS2078/Scer\GAL4^Ser.PGF is a suppressor of wing phenotype of mib1⁴/mib1³ (Hamel et al., 2010) α4GT1^Scer\UAS.cHa, Scer\GAL4^Ser.PGF is a suppressor of wing phenotype of Scer\GAL4^Ser.PGF, mib1^{C1205S.Scer\UAS} (Hamel et al., 2010, Hamel et al., 2010) α4GT2^Scer\UAS.cHa, Scer\GAL4^Ser.PGF is a suppressor of wing phenotype of Scer\GAL4^Ser.PGF, mib1^{C1205S.Scer\UAS} (Hamel et al., 2010, Hamel et al., 2010)
NOT Suppressor of
Statement Reference Scer\GAL4^Ser.PGF/Ser^Scer\UAS.cSa is a non-suppressor of wing disc phenotype of mib1^EY97600/mib1³ (Le Borgne et al., 2005) α4GT1^GS2078/Scer\GAL4^Ser.PGF is a non-suppressor of wing phenotype of mib1³/Df(3L)mib1² (Hamel et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	mib1^Scer\UAS.cLa/Scer\GAL4^Ser.PGF rescues mib1³/Df(3L)mib1² (Le Borgne et al., 2005) mib1^{Scer\UAS.T:Avic\GFP-EYFP}/Scer\GAL4^Ser.PGF rescues mib1³/Df(3L)mib1² (Le Borgne et al., 2005) Scer\GAL4^Ser.PGF/EcR^A.Scer\UAS rescues EcR^{B1-ΔC655.F645A.Scer\UAS} (Cherbas et al., 2003)
Comments
Stocks ( 1 )
Bloomington	6791 w^*; P{Ser-GAL4.GF}1 P{Ser-GAL4.GF}2
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	Gal4^Ser1 (Certel et al., 2000) GAL4^Ser.2xP GAL4^Ser.PG Scer\GAL4^Ser.2xP Scer\GAL4^Ser.PGF
Name Synonym	Serrate promoter construct of Gu and Fleming (Hukriede et al., 1997)
Secondary FlyBase IDs
	FBal0063364
References ( 13 )
Research paper	Kim et al., 2012, PLoS ONE 7(4): e34016 Negative Regulation of EGFR/MAPK Pathway by Pumilio in Drosophila melanogaster. [FBrf0218126] Turki-Judeh and Courey, 2012, PLoS ONE 7(2): e30610 The unconserved groucho central region is essential for viability and modulates target gene specificity. [FBrf0217447] Hamel et al., 2010, J. Cell Biol. 188(4): 581--594 Notch ligand activity is modulated by glycosphingolipid membrane composition in Drosophila melanogaster. [FBrf0210025] Datta et al., 2009, Dev. Dyn. 238(9): 2202--2210 Restriction of ectopic eye formation by Drosophila teashirt and tiptop to the developing antenna. [FBrf0208704] Bardin and Schweisguth, 2006, Dev. Cell 10(2): 245--255 Bearded family members inhibit Neuralized-mediated endocytosis and signaling activity of Delta in Drosophila. [FBrf0190191] Kim et al., 2006, genesis 44(11): 541--549 Ectopic expression of Tollo/Toll-8 antagonizes Dpp signaling and induces cell sorting in the Drosophila wing. [FBrf0192910] Le Borgne et al., 2005, PLoS Biol. 3(4): e96 Two distinct E3 ubiquitin ligases have complementary functions in the regulation of Delta and Serrate signaling in Drosophila. [FBrf0184230] Cherbas et al., 2003, Development 130(2): 271--284 EcR isoforms in Drosophila: testing tissue-specific requirements by targeted blockade and rescue. [FBrf0155708] Certel et al., 2000, Development 127(14): 3173--3183 Restricted patterning of vestigial expression in Drosophila wing imaginal discs requires synergistic activation by both Mad and the Drifter POU domain transcription factor. [FBrf0128425] Zeng et al., 1998, Proc. Natl. Acad. Sci. U.S.A. 95(18): 10637--10642 The Drosophila LIM-only gene, dLMO, is mutated in beadex alleles and might represent an evolutionarily conserved function in appendage development. [FBrf0104534] Fleming et al., 1997, Development 124(15): 2973--2981 Serrate-mediated activation of Notch is specifically blocked by the product of the gene fringe in the dorsal compartment of the Drosophila wing imaginal disc. [FBrf0094729] Hukriede et al., 1997, Development 124(17): 3427--3437 A dominant-negative form of Serrate acts as a general antagonist of Notch activation. [FBrf0098268]
Personal communication to FlyBase	Fleming, 2003.7, P{Ser-GAL4.GF}1 and P{Ser-GAL4.GF}2. P{Ser-GAL4.GF}1 and P{Ser-GAL4.GF}2. [FBrf0159896]

Allele Scer\GAL4Ser.PGF

Allele Scer\GAL4^Ser.PGF