Allele Scer\GAL4^15J2

General Information
Symbol	Scer\GAL4^15J2	Species	S. cerevisiae
Name		FlyBase ID	FBal0065227
Feature type	allele	Associated gene	Scer\GAL4

Allele class
Mutagen
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms viable
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	P{GawB}15J2 (Hidalgo and Brand, 1997, Hidalgo and Booth, 2000, Landgraf et al., 2003, Georgiou and Tear, 2002, Connell-Crowley, 2000, Takizawa and Hotta, 2001, Hiramoto et al., 2000, Lanoue et al., 2000, Simpson et al., 2000, Miguel-Aliaga and Thor, 2004, Kuzina et al., 2011, Hiramoto and Hiromi, 2006)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective, with lea^{Scer\UAS.T:Hsap\MYC} (Kinrade and Hidalgo, 2004) neuroanatomy defective \| embryonic stage, with comm^Scer\UAS.cUa (Hiramoto and Hiromi, 2006) neuroanatomy defective \| embryonic stage, with Gαq^{Q203L.Scer\UAS} (Hiramoto and Hiromi, 2006) neuroanatomy defective \| embryonic stage, with robo^{Scer\UAS.T:Hsap\MYC} (Hiramoto and Hiromi, 2006)
Phenotype Manifest In
	axon (Hidalgo and Brand, 1997) dMP2 neuron, with comm^Scer\UAS.cUa (Hiramoto and Hiromi, 2006) dMP2 neuron, with Gαq^{Q203L.Scer\UAS} (Hiramoto and Hiromi, 2006) dMP2 neuron, with lea^EP2582 (Simpson et al., 2000) dMP2 neuron, with robo^Scer\UAS.cKa (Simpson et al., 2000) dMP2 neuron, with robo^{Scer\UAS.T:Hsap\MYC} (Hiramoto and Hiromi, 2006) fascicle \| embryonic stage, with Scer\GAL4^s.gcm (Hidalgo and Booth, 2000) interface glial cell, with vn^{dsRNA.Scer\UAS} (Hidalgo et al., 2001) longitudinal connective, with Scer\GAL4^s.gcm (Hidalgo and Booth, 2000) medial longitudinal fascicle, with lea^{Scer\UAS.T:Hsap\MYC} (Kinrade and Hidalgo, 2004) pCC neuron, with Scer\GAL4^s.gcm (Hidalgo and Booth, 2000) vMP2 neuron, with lea^EP2582 (Simpson et al., 2000) vMP2 neuron, with robo^Scer\UAS.cKa (Simpson et al., 2000)
Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Gαq^{Q203L.Scer\UAS}, Scer\GAL4^15J2 has neuroanatomy defective \| embryonic stage phenotype, suppressible by fra³ (Hiramoto and Hiromi, 2006) Gαq^{Q203L.Scer\UAS}, Scer\GAL4^15J2 has neuroanatomy defective \| embryonic stage phenotype, suppressible by robo^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^15J2 (Hiramoto and Hiromi, 2006, Hiramoto and Hiromi, 2006) Gαq^{Q203L.Scer\UAS}, Scer\GAL4^15J2 has neuroanatomy defective \| embryonic stage phenotype, suppressible by Ts(1Lt;YSt)B118+Ts(1Rt;YLt)T9 (Hiramoto and Hiromi, 2006)
Enhancer of
Statement Reference cpb^Scer\UAS.cWa/Scer\GAL4^15J2 is an enhancer of neuroanatomy defective \| recessive phenotype of N^l1N-ts1 (Kuzina et al., 2011) lea^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^15J2 is an enhancer of neuroanatomy defective phenotype of gcm^ΔP1 (Kinrade and Hidalgo, 2004) lea^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^15J2 is an enhancer of neuroanatomy defective phenotype of gcm^ΔP1, robo³ (Kinrade and Hidalgo, 2004) lea^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^15J2 is an enhancer of neuroanatomy defective phenotype of robo³ (Kinrade and Hidalgo, 2004) Scer\GAL4^15J2/Dl^{ΔIC.Scer\UAS} is an enhancer of neuroanatomy defective \| recessive phenotype of N^l1N-ts1 (Kuzina et al., 2011) Scer\GAL4^15J2/trio^{GEF1.Scer\UAS.T:Hsap\MYC} is an enhancer of neuroanatomy defective \| recessive phenotype of N^l1N-ts1 (Kuzina et al., 2011) Scer\GAL4^15J2/Zzzz\actA^{FP4mito.Scer\UAS.T:Avic\GFP-EGFP} is an enhancer of neuroanatomy defective \| recessive phenotype of N^l1N-ts1 (Kuzina et al., 2011)
Suppressor of
Statement Reference ena^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^15J2 is a suppressor of neuroanatomy defective \| recessive phenotype of N^l1N-ts1 (Kuzina et al., 2011) robo^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^15J2 is a suppressor of neuroanatomy defective \| embryonic stage phenotype of Gαq^{Q203L.Scer\UAS}, Scer\GAL4^15J2 (Hiramoto and Hiromi, 2006, Hiramoto and Hiromi, 2006) Scer\GAL4^15J2/Mmus\Itpr1^{sponge.Scer\UAS.T:Avic\GFP} is a suppressor \| partially of neuroanatomy defective \| embryonic stage phenotype of robo¹ (Hiramoto and Hiromi, 2006) Scer\GAL4^15J2/Rac1^N17.Scer\UAS is a suppressor of neuroanatomy defective \| recessive phenotype of N^l1N-ts1 (Kuzina et al., 2011)
NOT Suppressor of
Statement Reference Scer\GAL4^15J2/Mmus\Itpr1^{mut.sponge.Scer\UAS.T:Avic\GFP} is a non-suppressor of neuroanatomy defective \| embryonic stage phenotype of robo¹ (Hiramoto and Hiromi, 2006) Scer\GAL4^15J2/Su(H)^{Scer\UAS.T:Hsap\MYC,T:Hsim\VP16} is a non-suppressor of neuroanatomy defective \| recessive phenotype of N^l1N-ts1 (Kuzina et al., 2011)
Phenotype Manifest In
Suppressed by
Statement Reference Gαq^{Q203L.Scer\UAS}, Scer\GAL4^15J2 has dMP2 neuron phenotype, suppressible by fra³ (Hiramoto and Hiromi, 2006) Gαq^{Q203L.Scer\UAS}, Scer\GAL4^15J2 has dMP2 neuron phenotype, suppressible by robo^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^15J2 (Hiramoto and Hiromi, 2006, Hiramoto and Hiromi, 2006) Gαq^{Q203L.Scer\UAS}, Scer\GAL4^15J2 has dMP2 neuron phenotype, suppressible by Ts(1Lt;YSt)B118+Ts(1Rt;YLt)T9 (Hiramoto and Hiromi, 2006)
Enhancer of
Statement Reference cpb^Scer\UAS.cWa/Scer\GAL4^15J2 is an enhancer of pioneer neuron phenotype of N^l1N-ts1 (Kuzina et al., 2011) lea^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^15J2 is an enhancer of dMP2 neuron phenotype of gcm^ΔP1, robo³ (Kinrade and Hidalgo, 2004) lea^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^15J2 is an enhancer of intermediate longitudinal fascicle phenotype of robo³ (Kinrade and Hidalgo, 2004) lea^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^15J2 is an enhancer of lateral longitudinal fascicle phenotype of robo³ (Kinrade and Hidalgo, 2004) lea^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^15J2 is an enhancer of medial longitudinal fascicle phenotype of robo³ (Kinrade and Hidalgo, 2004) lea^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^15J2 is an enhancer of vMP2 neuron phenotype of gcm^ΔP1, robo³ (Kinrade and Hidalgo, 2004) Scer\GAL4^15J2/Dl^{ΔIC.Scer\UAS} is an enhancer of dMP2 neuron \| embryonic stage phenotype of N^l1N-ts1 (Kuzina et al., 2011) Scer\GAL4^15J2/Dl^{ΔIC.Scer\UAS} is an enhancer of vMP2 tract \| embryonic stage phenotype of N^l1N-ts1 (Kuzina et al., 2011) Scer\GAL4^15J2/trio^{GEF1.Scer\UAS.T:Hsap\MYC} is an enhancer of pioneer neuron phenotype of N^l1N-ts1 (Kuzina et al., 2011) Scer\GAL4^15J2/Zzzz\actA^{FP4mito.Scer\UAS.T:Avic\GFP-EGFP} is an enhancer of pioneer neuron phenotype of N^l1N-ts1 (Kuzina et al., 2011)
Suppressor of
Statement Reference ena^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^15J2 is a suppressor of pioneer neuron phenotype of N^l1N-ts1 (Kuzina et al., 2011) robo^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^15J2 is a suppressor of dMP2 neuron phenotype of Gαq^{Q203L.Scer\UAS}, Scer\GAL4^15J2 (Hiramoto and Hiromi, 2006, Hiramoto and Hiromi, 2006) Scer\GAL4^15J2/Mmus\Itpr1^{sponge.Scer\UAS.T:Avic\GFP} is a suppressor \| partially of dMP2 neuron phenotype of robo¹ (Hiramoto and Hiromi, 2006) Scer\GAL4^15J2/Rac1^N17.Scer\UAS is a suppressor of pioneer neuron phenotype of N^l1N-ts1 (Kuzina et al., 2011)
NOT Suppressor of
Statement Reference Scer\GAL4^15J2/Mmus\Itpr1^{mut.sponge.Scer\UAS.T:Avic\GFP} is a non-suppressor of dMP2 neuron phenotype of robo¹ (Hiramoto and Hiromi, 2006) Scer\GAL4^15J2/Su(H)^{Scer\UAS.T:Hsap\MYC,T:Hsim\VP16} is a non-suppressor of pioneer neuron phenotype of N^l1N-ts1 (Kuzina et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^15J2/N^Scer\UAS.cUa rescues N^l1N-ts1 (Kuzina et al., 2011)
Partially rescues	robo^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^15J2 partially rescues robo¹ (Hiramoto and Hiromi, 2006) Scer\GAL4^15J2/N^{1893-2155.Scer\UAS} partially rescues N^l1N-ts1 (Kuzina et al., 2011)
Fails to rescue	Scer\GAL4^15J2/fra^Scer\UAS.cKa fails to rescue fra³/fra⁴ (Hiramoto et al., 2000) Scer\GAL4^15J2/N^{FLNΔ10-12.Scer\UAS} fails to rescue N^l1N-ts1 (Kuzina et al., 2011)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	15J2(MP2 GAL4) (Kinrade and Hidalgo, 2004) GAL4^15J2 Scer\GAL4^15J2
Name Synonym
Secondary FlyBase IDs

References ( 14 )
Research paper	Kuzina et al., 2011, Development 138(9): 1839--1849 How Notch establishes longitudinal axon connections between successive segments of the Drosophila CNS. [FBrf0213493] Hiramoto and Hiromi, 2006, Nat. Neurosci. 9(1): 58--66 ROBO directs axon crossing of segmental boundaries by suppressing responsiveness to relocalized Netrin. [FBrf0191279] Kinrade and Hidalgo, 2004, Neuron Glia Biol. 1: 101--112 Lateral neuron-glia interactions steer the response of axons to the Robo code. [FBrf0188594] Miguel-Aliaga and Thor, 2004, Development 131(24): 6093--6105 Segment-specific prevention of pioneer neuron apoptosis by cell-autonomous, postmitotic Hox gene activity. [FBrf0180186] Landgraf et al., 2003, Dev. Biol. 260(1): 207--225 Charting the Drosophila neuropile: a strategy for the standardised characterisation of genetically amenable neurites. [FBrf0160715] Georgiou and Tear, 2002, Development 129(12): 2947--2956 Commissureless is required both in commissural neurones and midline cells for axon guidance across the midline. [FBrf0151911] Hidalgo et al., 2001, Dev. Cell 1(5): 679--690 The Drosophila neuregulin vein maintains glial survival during axon guidance in the CNS. [FBrf0141461] Takizawa and Hotta, 2001, Dev. Genes Evol. 211(1): 30--36 Pathfinding analysis in a glia-less gcm mutant in Drosophila. [FBrf0134555] Hidalgo and Booth, 2000, Development 127(2): 393--402 Glia dictate pioneer axon trajectories in the Drosophila embryonic CNS. [FBrf0123048] Lanoue et al., 2000, Genome 43(3): 564--573 Genetic analysis of vein function in the Drosophila embryonic nervous system. [FBrf0128541] Simpson et al., 2000, Cell 103(7): 1019--1032 Short-range and long-range guidance by Slit and its Robo receptors: a combinatorial code of Robo receptors controls lateral position. [FBrf0132257] Hidalgo and Brand, 1997, Development 124(17): 3253--3262 Targeted neuronal ablation: the role of pioneer neurons in guidance and fasciculation in the CNS of Drosophila. [FBrf0098258]
Supplementary material	Connell-Crowley, 2000, Curr. Biol. 10(10): The cyclin-dependent kinase cdk5 controls multiple aspects of axon patterning in vivo. [FBrf0128834]
Letter	Hiramoto et al., 2000, Nature 406(6798): 886--889 The Drosophila Netrin receptor Frazzled guides axons by controlling Netrin distribution. [FBrf0129858]

Allele Scer\GAL415J2

Allele Scer\GAL4^15J2