Open Close
General Information
Symbol
Dmel\CycAC8LR1
Species
D. melanogaster
Name
FlyBase ID
FBal0065308
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
CycAC8, Cyclin Ac8LR1
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    CycAC8LR1 homozygous somatic clones in third instar larval eye discs do not enter S transition during the second mitotic wave.

    Sleep defects are observed in CycAEY11746 mutant heterozygotes.

    In CycAC8LR1 mutant embryos, tracheal branches are formed and migrate to their destinations with only half the normal number of cells and accomplish a standard tube diameter and length, with ganglionic branches invading the ventral nerve cord as in the wild-type.

    The number of glial cells is reduced in mutant embryos compared to controls.

    Progression through mitosis 16 does not occur in CycAC8LR1 mutant embryos and all epidermal cells remain in G2.

    CycAC8LR1 embryos show a general failure of epidermal cells to progress through mitosis 16. There are some mitotic cells that progress through this cycle in CycAC8LR1 embryos, but these are restricted mainly to the prospective anterior spiracle region in the epidermis and are unusual in that they progress through an additional division cycle compared to cells in wild-type embryos, so that the mutant cells complete 17 rounds of mitosis instead of 16.

    The terminal mitoses of ganglion mother cells are preferentially inhibited in CycAC8LR1 embryos. In contrast, the asymmetric neuroblast divisions, which are not terminal mitoses, are less inhibited in these mutants.

    In CycAC8LR1 embryos, the GMC4-2a cell differentiates into only one RP2 neuron, instead of a RP2 neuron and a RP2sib neuron.

    Mitosis 16 does not occur in the epidermal layer in mutant embryos, resulting in embryos with fewer but bigger cells compared to wild type.

    Mutant embryos lack eve expressing pericardial cells and have only a single odd expressing pericardial cell per hemisegment. DA1 muscles form as in wild-type. The DO2 founders are also present. A halving is also seen in the number of myocardial cells seen in segments A2 to A7, though the number seen in segments T3 to A1 remain normal.

    In CycAC8LR1 mutants, cell cycle arrest occurs at mitosis 16.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhanced by
    Statement
    Reference
    Suppressed by
    NOT suppressed by
    Statement
    Reference

    CycAC8LR1 has sleep defective | dominant phenotype, non-suppressible by CycAC8LR1/CycA[+]

    Enhancer of
    NOT Enhancer of
    Statement
    Reference

    CycAC8LR1/CycA[+] is a non-enhancer of sleep defective | dominant phenotype of DATfmn

    CycAC8LR1 is a non-enhancer of visible phenotype of upd1GMR.PB

    Suppressor of
    Statement
    Reference

    CycAC8LR1/CycA[+] is a suppressor of neuroanatomy defective phenotype of babo32/babo52

    CycAC8LR1/CycA[+] is a suppressor of visible phenotype of E(mus304)[+]/DNApol-α180Emus304, mei-4129D

    CycAC8LR1/CycA[+] is a suppressor of increased cell death phenotype of E(mus304)[+]/DNApol-α180Emus304, mei-4129D

    CycAC8LR1/CycA[+] is a suppressor of increased cell death phenotype of shtd1

    NOT Suppressor of
    Statement
    Reference

    CycAC8LR1/CycA[+] is a non-suppressor of sleep defective | dominant phenotype of CycAC8LR1

    CycAC8LR1 is a non-suppressor of visible phenotype of upd1GMR.PB

    Other
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference

    CycAC8LR1 has cardioblast phenotype, enhanceable by CycB2

    Suppressed by
    Statement
    Reference
    NOT suppressed by
    Statement
    Reference
    NOT Enhancer of
    Statement
    Reference
    Suppressor of
    Statement
    Reference

    CycAC8LR1/CycA[+] is a suppressor | partially of lch1 neuron phenotype of catounspecified

    CycAC8LR1/CycA[+] is a suppressor of axon & eye photoreceptor cell phenotype of babo32/babo52

    CycAC8LR1/CycA[+] is a suppressor of lamina phenotype of babo32/babo52

    CycAC8LR1/CycA[+] is a suppressor of eye phenotype of E(mus304)[+]/DNApol-α180Emus304, mei-4129D

    CycAC8LR1/CycA[+] is a suppressor of wing disc phenotype of E(mus304)[+]/DNApol-α180Emus304, mei-4129D

    CycAC8LR1/CycA[+] is a suppressor of eye phenotype of shtd1

    CycAC8LR1/CycA[+] is a suppressor of ommatidium phenotype of shtd1

    CycAC8LR1 is a suppressor | partially of larval cuticle & larval abdomen phenotype of Cks30AKO/Cks30ARA74

    CycAC8LR1/CycA[+] is a suppressor of aster | embryonic cycle 5 phenotype of CycB+t10

    CycAC8LR1/CycA[+] is a suppressor of aster | embryonic cycle 6 phenotype of CycB+t10

    CycAC8LR1/CycA[+] is a suppressor of aster | embryonic cycle 7 phenotype of CycB+t10

    CycAC8LR1/CycA[+] is a suppressor of mitotic domain 1 | embryonic cycle 14 phenotype of CycB+t10

    CycAC8LR1/CycA[+] is a suppressor of mitotic domain 1 | embryonic cycle 10 phenotype of CycB+t10

    NOT Suppressor of
    Statement
    Reference

    CycAC8LR1 is a non-suppressor of eye phenotype of upd1GMR.PB

    Other
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    One copy of CycAC8LR1 enhances the sleep defects seen in flies co-expressing taraNIG.6889R and taraJF01421 under the control of Scer\GAL4elav.PU.

    One copy of CycAC8LR1 does not enhance the sleep defects seen in DATfmn mutant heterozygotes.

    One copy of Cdk1GT-000294 does not suppress the sleep defects seen in CycAC8LR1 mutant flies.

    The number of glial cells in rapie28 CycAC8LR1 double mutant embryos is the same as in wild-type embryos, but they fail to migrate.

    The duplication of v'ch1 neurons that is seen in catounspecified embryos is partially suppressed by CycAC8LR1/+.

    Expression of CycJScer\UAS.C.T:Avic\GFP-EGFP in epidermal regions under the control of Scer\GAL4prd.RG1 in CycAC8LR1 mutants does not rescue the cell cycle arrest in G2 of cycle 16.

    The photoreceptor axon targeting and lamina neuron defects seen in babo32/babo52 mutants are substantially suppressed by CycAC8LR1/+.

    The lethality seen in mei-4129D males in which excision of an insertion of P{hswa} is induced during development is partly suppressed by CycAC8LR1/+.

    The lethality seen in mei-4129D males in which excision of an insertion of P{hswa} is induced during development is completely suppressed if the female parent carries CycAC8LR1 and if the male progeny carries both CycAC8LR1 and CycB2.

    The rough eye phenotype seen in mei-4129D ; DNApol-α180Emus304/+ adults is completely rescued by CycAC8LR1/+. The increased apoptosis seen in the wing discs of mei-4129D ; DNApol-α180Emus304/+ mutants is rescued by CycAC8LR1/+ to levels indistinguishable from those of mei-4129D single mutants or mei-4129D ; CycAC8LR1/+ mutants.

    Expression of cdc2AF.hs in CycAC8LR1 mutant embryos fails to restore mitosis 16 in the epidermis.

    Coexpression of the transgenes, cdc2AF.hs, CycBΔ90, and CycB3Δ, restores the entry of epidermal cells in CycAC8LR1 embryos into mitosis 16. After entry into mitosis, the epidermal cells are unable to complete this division.

    Coexpression of the transgenes, cdc2hs.PS, CycBΔ90, and CycB3Δ, does not restore the entry of epidermal cells in CycAC8LR1 embryos into mitosis 16. Likewise, coexpression of stghs.PE2, CycBΔ90 and CycB3Δ does not suppress the phenotype, nor does the coexpression of cdc2AF.hs, CycBhs.PK and CycB3hs.PS.

    The epidermis of rapie28; CycAC8LR1 double mutant embryos shows an extra mitotic division cycle as with CycAC8LR1 single mutant embryos. This is in contrast to CycAC8LR1 single mutant embryos, where the 16th mitotic division does not occur. In addition to the extra round of mitosis, rapie28; CycAC8LR1 double mutant embryonic epidermal cells show abnormal anaphase and telophase figures with chromatin bridges.

    Expression of CycEScer\UAS.cLa under the control of Scer\GAL4prd.RG1 suppresses the mitotic phenotype of CycAC8LR1 embryos so that cells progress through mitosis 16 and a subsequent S phase.

    In CycAC8LR1; CycEAR95 double mutant embryos, cells arrest before mitosis 15, a phenotype not seen in either single mutant.

    The mitotic embryonic phenotype of CycAC8LR1 single mutants, in which mitosis 16 does not occur, is not suppressed in dap4; CycAC8LR1 double mutants. However, the double mutants do enter another S phase after the stage at which mitosis occurs in equivalent wild-type embryos.

    In numb15; CycAC8LR1 double mutants, GMC4-2a differentiates into a RP2sib in 85% of cases, while in numbS52F; CycAC8LR1 double mutants, an RP2 neuron is generated in 99% of cases.

    Reduction of CycA, in CycAC8LR1 heterozygotes partially suppresses the Cks30AKO/Cks30ARA74 phenotype.

    Expression of CycA::torScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4prd.RG1 in a CycAC8LR1 background results in only a slight increase in cell number in the Scer\GAL4prd.RG1 expression domain compared to cells outside the Scer\GAL4prd.RG1 expression domain, although there is a significant increase in the number of nuclei in the Scer\GAL4prd.RG1 expression domain (a high number of binucleate cells are seen). Expression of CycBScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4prd.RG1 does not rescue the cell division defects seen in CycAC8LR1 embryos; there is no significant increase in cell number in the Scer\GAL4prd.RG1 expression domain. Expression of CycBScer\UAS.T:Ivir\HA1,T:SV40\nls2 under the control of Scer\GAL4prd.RG1 does not rescue the cell division defects seen in CycAC8LR1 embryos; there is no significant increase in cell number in the Scer\GAL4prd.RG1 expression domain. Expression of Rca1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4prd.RG1 partially rescues the cell division defects seen in CycAC8LR1 embryos; there is some increase in cell number in the Scer\GAL4prd.RG1 expression domain.

    Adult viability of CycD1/Y ; CycAC8LR1/+ males is 89% of expected value.

    CycAC8LR1, CycB2 double mutants arrest mesodermal division at mitosis 15 a more severe phenotype than CycAC8LR1 alone. Also a further reduction in the number of myocardial cells is seen. In numb1, CycAC8LR1 double mutant embryos the formation of one non-myogenic eve expressing cell per segment is seen, apparently at the expense of DA1 muscle formation.

    Cell cycle arrest in CycAC8LR1/CycAC8LR1; Rca12/Rca12 embryos, occurs earlier (cycle 15) than in either single mutant (both arrest at cycle 16).

    CycB3L6540 CycAC8LR1 double mutants show delayed chromatin condensation during prophase during mitosis 15 and rare mitotic cells with aberrant multipolar spindles at stage 13. CycB3L6540 CycB- (Df(2R)599-5) CycAC8LR1 triple mutants are blocked at mitosis 15. 50% of embryos complete syncytial cycles without obvious defects, although many embryos were highly abnormal. Endoreduplicating cells still undergo DNA synthesis, though mitotic cells of the CNS do not.

    Df(1)bi-D3/Y ; CycAC8LR1/CycAC8LR1 double mutant embryos show normal progression through mitosis 16 followed by an ectopic S phase.

    Xenogenetic Interactions
    Statement
    Reference

    The rough-eye phenotype observed in animals expressing Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed in a CycAC8LR1/+ background.

    Complementation and Rescue Data
    Comments

    Expression of CycAScer\UAS.cWa driven by Scer\GAL4prd.RG1 rescues the mitosis 16 in epidermal cells in CycAC8LR1 embryos in the segments where CycAScer\UAS.cWa is expressed. The intervening segments show the CycAC8LR1 mitotic phenotype.

    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (8)
    References (32)