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General Information
Symbol
Dmel\wgUAS.cKa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Krause
FlyBase ID
FBal0065586
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-wg, PUAS-wg
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Wild-type wg is expressed under the control of UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies overexpressing wgScer\UAS.cKa under the control of Scer\GAL4Hml.Δ in the hemocytes develop normally, with no obvious defects after they hatch. Expression of hhScer\UAS.cIa does not protect against infection with group B streptococcus.

Ectopic expression of wgScer\UAS.cKa in males under the control of Scer\GAL4dsx-GAL4 results in the formation of a female-like adult abdominal segment 7 cuticle. The mutant males develop excess tissue posterior to the A6 tergite. This excess tissue is sclerotised and pigmented, suggesting that it adopts anterior cuticle fate. Ventral histoblasts are also transformed generating an A7 sternite. Additionally, ectopic wgScer\UAS.cKa expression results in partial transformation of the male A6 sternite toward more anterior identity. Compared with wild-type males, the A6 sternite of males expressing wgScer\UAS.cKa under the control of Scer\GAL4dsx-GAL4 is more rectangular and develop supernumerary bristles. The transformed A7 cuticle of the mutant males is narrower than more anterior segments and devoid of bristles.

Expression of wgScer\UAS.cKa under the control of Scer\GAL4GMR.PF causes a dramatic reduction in adult eye size.

Expression of wgScer\UAS.cKa under the control of Scer\GAL4ey.PH results in a no eye phenotype.

Expression of wgScer\UAS.cKa in the developing eye, under the control of Scer\GAL4GMR.PF, results in a severe reduction in adult eye size.

Expression of wgScer\UAS.cKa in alternating embryonic segements, driven by Scer\GAL4h-1J3, results in the formation of ectopic naked cuticle.

When wgScer\UAS.cKa is driven by Scer\GAL4GMR.PF mutant animals have a dramatically reduced eye.

Adults expressing wgScer\UAS.cKa under the control of Scer\GAL4GMR.PF have reduced eyes.

When expression is driven by Scer\GAL4da.G32 a completely naked embryonic cuticle results. When expression is driven in the eye with Scer\GAL4GMR.PF, a glassy reduced eye results. When expression is driven in the eye with Scer\GAL4GMR.PF, the eye becomes slightly roughened.

Expression of wgScer\UAS.cKa under the control of Scer\GAL4prd.RG1 in wild-type embryos results in deletion of denticle bands at 16oC and 25oC.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Expression of wgScer\UAS.cKa under the control of Scer\GAL4prd.RG1 restores naked cuticle in dlpA187 embryos derived from dlpA187 female germline clones.

Expression of spendsRNA.Scer\UAS partially suppresses the reduction in eye size seen when wgScer\UAS.cKa is expressed under the control of Scer\GAL4GMR.PF.

Expression of nitodsRNA.Scer\UAS.cCa partially suppresses the reduction in eye size seen when wgScer\UAS.cKa is expressed under the control of Scer\GAL4GMR.PF.

Co-expression of nitodsRNA.Scer\UAS.cCa and spendsRNA.Scer\UAS partially suppresses the reduction in eye size seen when wgScer\UAS.cKa is expressed under the control of Scer\GAL4GMR.PF. The suppression is greater than is seen when either transgene is expressed alone.

Coexpression of either CtBPGSA132 or CtBPGSA396, suppress the eye phenotype of Scer\GAL4GMR.PF>wgScer\UAS.cKa flies.

When wgScer\UAS.cKa or hhScer\UAS.cIa is driven by Scer\GAL4prd.RG1 in sll7E18 animals suppression of the segment polarity phenotype is seen in those domains that express Scer\GAL4prd.RG1.

When wgScer\UAS.cKa is expressed under the control of Scer\GAL4prd.RG1 in a an embryo that is zygotically and maternally mutant for frc00073, naked cuticle is restored in a typical pair-rule pattern.

When expression of AxnA1.Scer\UAS along with wgScer\UAS.cKa is driven in the eye by Scer\GAL4GMR.PF the eye phenotype is slightly reduced and roughened, i.e. intermediate between that of AxnA1.Scer\UAS or wgScer\UAS.cKa alone.

Expression of wgScer\UAS.cKa under the control of Scer\GAL4prd.RG1 in sgl08310 embryos induces naked cuticle at 25oC. Narrow regions of naked cuticle are produced when wgScer\UAS.cKa is expressed under the control of Scer\GAL4prd.RG1 in sgl08310 embryos at 16oC.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
wgScer\UAS.cKa
wgUAS.cKa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Krause
Secondary FlyBase IDs
    References (21)