FB2025_01 , released February 20, 2025
Allele: Dmel\wgUAS.cHa.Tag:HA
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General Information
Symbol
Dmel\wgUAS.cHa.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0065588
Feature type
allele
Associated gene
Dmel\wg
Associated Insertion(s)
Carried in Construct
P{UAS-wg.H.T:HA1}
Also Known As
UAS-wg, UAS-wg-HA, UAS-wg::HA, UAS-wg+
Key Links
Transgenic product class
cDNA
(Hays et al., 1997)
wild_type
(Hays et al., 1997)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Hays et al., 1997)
wild_type
(Hays et al., 1997)
Mutagen
FLPase
(Hays et al., 1997)
Progenitor genotype
wgFRT.UAS.cHa.Tag:HA
(Hays et al., 1997)
Carried in construct
P{UAS-wg.H.T:HA1}
Cytology
Description

The FRT cassette has been excised, juxtaposing the UASt regulatory sequences and wg coding sequences tagged at amino acid residue 111 with Tag:HA.

(Hays et al., 1997)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
wg
Tagged with
Tag:HA
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal cell number | oogenesis, with Scer\GAL4C587

      (Waghmare et al., 2020)

      abnormal endomitotic cell cycle

      (Taniue et al., 2010)

      abnormal flight, with Scer\GAL4elav-C155

      (Marcogliese et al., 2022)

      abnormal flight, with Scer\GAL4nSyb.PS

      (Marcogliese et al., 2022)

      abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4elav-C155

      (Marcogliese et al., 2022)

      abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4nSyb.PS

      (Marcogliese et al., 2022)

      abnormal neuroanatomy | adult stage | progressive, with Scer\GAL4nSyb.PS

      (Marcogliese et al., 2022)

      abnormal neuroanatomy | larval stage, with Scer\GAL4nSyb.PS

      (Huang et al., 2018)

      abnormal neuroanatomy | somatic clone | third instar larval stage, with Scer\GAL4elav.PH

      (Haussmann et al., 2008)

      abnormal size | adult stage, with Scer\GAL4Mef2.PR

      (Lee et al., 2014)

      abnormal size | third instar larval stage, with Scer\GAL4DcG

      (Lee et al., 2014)

      increased cell number | wandering third instar larval stage, with Scer\GAL4btl.PS

      (Pitsouli and Perrimon, 2013)

      increased occurrence of cell division | adult stage, with Scer\GAL4NP7397

      (Perea et al., 2017)

      increased occurrence of cell division | third instar larval stage, with Scer\GAL4byn-Gal4

      (Takashima et al., 2008)

      lethal, with Scer\GAL4pb.PJ

      (Joulia et al., 2005)

      lethal - all die before end of larval stage | RU486 conditional, with Scer\GAL4Act5C.Switch.PR

      (Shen et al., 2009)

      lethal - all die before end of P-stage, with Scer\GAL4nub-AC-62

      (Marcogliese et al., 2022)

      lethal - all die during P-stage, with Scer\GAL4DcG

      (Lee et al., 2014)

      short lived, with Scer\GAL4elav-C155

      (Marcogliese et al., 2022)

      short lived, with Scer\GAL4nSyb.PS

      (Marcogliese et al., 2022)

      short lived | RU486 conditional, with Scer\GAL4Act5C.Switch.PR

      (Shen et al., 2009)

      some die during larval stage | RU486 conditional, with Scer\GAL4Act5C.Switch.PR

      (Shen et al., 2009)

      visible | adult stage, with Scer\GAL4ptc.PU

      (Worley et al., 2018)
      Phenotype Manifest In

      adult abdominal fat mass, with Scer\GAL4Mef2.PR

      (Lee et al., 2014)

      adult anterior hindgut, with Scer\GAL4byn-Gal4, Scer\GAL80ts.αTub84B

      (Takashima et al., 2008)

      axon | adult stage | decreased number, with Scer\GAL4nSyb.PS

      (Marcogliese et al., 2022)

      bouton | increased number, with Scer\GAL4C164

      (Packard et al., 2002)

      bouton | increased number, with Scer\GAL4futsch-C380

      (Packard et al., 2002)

      embryonic/first instar larval cuticle, with Scer\GAL4e22c

      (Chao et al., 2003)

      embryonic/larval crystal cell | third instar larval stage, with Scer\GAL4lz-gal4

      (Sinenko et al., 2009)

      embryonic/larval fat body | third instar larval stage, with Scer\GAL4DcG

      (Lee et al., 2014)

      embryonic/larval hindgut, with Scer\GAL4byn-Gal4, Scer\GAL80ts.αTub84B

      (Takashima et al., 2008)

      embryonic/larval lymph gland | third instar larval stage, with Scer\GAL4Antp-10

      (Sinenko et al., 2009)

      embryonic/larval neuromuscular junction, with Scer\GAL4nSyb.PS

      (Huang et al., 2018)

      embryonic/larval neuromuscular junction | somatic clone, with Scer\GAL4elav.PH

      (Haussmann et al., 2008)

      embryonic/larval salivary gland, with Scer\GAL4dpp.blk1

      (Taniue et al., 2010)

      escort cell | increased number, with Scer\GAL4C587

      (Waghmare et al., 2020)

      eye, with Scer\GAL4GMR.PF

      (Kryuchkov et al., 2011)

      female germline stem cell | decreased number, with Scer\GAL4C587

      (Waghmare et al., 2020)

      intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4NP7397

      (Perea et al., 2017)

      lipid droplet | adult stage, with Scer\GAL4Mef2.PR

      (Lee et al., 2014)

      lymph gland derived hemocyte | third instar larval stage, with Scer\GAL4dome-PG14

      (Sinenko et al., 2009)

      marginal nerve, with Scer\GAL4nSyb.PS

      (Marcogliese et al., 2022)

      NMJ bouton | somatic clone, with Scer\GAL4elav.PH

      (Haussmann et al., 2008)

      nucleus, with Scer\GAL4dpp.blk1

      (Taniue et al., 2010)

      ommatidium, with Scer\GAL4GMR.PF

      (Kryuchkov et al., 2011)

      stalk follicle cell | increased number, with Scer\GAL4bab1-Gal4

      (Wang and Page-McCaw, 2014)

      stalk follicle cell | increased number, with Scer\GAL4C587

      (Wang and Page-McCaw, 2014)

      terminal bouton | larval stage, with Scer\GAL4nSyb.PS

      (Huang et al., 2018)

      tracheolar cell | wandering third instar larval stage, with Scer\GAL4btl.PS

      (Pitsouli and Perrimon, 2013)

      ventral denticle belt, with Scer\GAL4e22c

      (Chao et al., 2003)

      ventral denticle belt | absent | embryonic stage, with Scer\GAL4VP16.mat.αTub67C, Scer\GAL4da.G32

      (Tan et al., 2024)

      wing | ectopic, with Scer\GAL4ptc.PU

      (Worley et al., 2018)
      Detailed Description
      Statement
      Reference

      The expression of wgUAS.cHa.Tag:HA under the control of Scer\GAL4nSyb.PS results in short lived adults with a progressive decrease in climbing ability; 25 days old adults also show decreased flight ability; peripheral wing nerves show axonal loss in aged flies (25 days post eclosion).

      The expression of wgUAS.cHa.Tag:HA under the control of Scer\GAL4elav-C155 results in short lived adults with a progressive decrease in climbing ability; 25 days old adults also show decreased flight ability.

      (Marcogliese et al., 2022)

      Expression of wgUAS.cHa.Tag:HA under the control of Scer\GAL4nSyb.PS significantly increases bouton number at the neuromuscular junction of muscle 4, compared to controls, with no effect on bouton size.

      (Huang et al., 2018)

      Expression of wgUAS.cHa.Tag:HA under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) does not lead to ectopic wings compared to controls.

      Restricted expression of wgUAS.cHa.Tag:HA to second but not third instar larvae (under the control of Scer\GAL4ptc.PU and tub-Gal80[ts]) leads to ectopic wings compared to controls.

      (Worley et al., 2018)

      Adult flies expressing wgScer\UAS.cHa.T:Ivir\HA1 driven by Scer\GAL4esg-NP7397 combined with tub-Gal80[ts] (for temporal control) display increase in midgut stem cell proliferation (assessed by number of pH3-positive cells).

      (Perea et al., 2017)

      Expression of wgScer\UAS.cHa.T:Ivir\HA1 under the Scer\GAL4svp.PT driver does not change ostia cell shape.

      (Trujillo et al., 2016)

      Adults expressing wgUAS.cHa.Tag:HA under the control of Scer\GAL4Mef2.PR have a reduced abdominal fat body with smaller lipid droplets than controls.

      Third instar larvae expressing wgUAS.cHa.Tag:HA under the control of Scer\GAL4DcG have a severely smaller fat body in the abdominal region.

      (Lee et al., 2014)

      Expression of wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4bab1-Gal4 results in increased numbers of stalk cells between egg chambers.

      Females expressing wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4C587 (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit expression to the adult stage) show an increase in the average number of cells in the interfollicle stalks compared to wild type.

      (Wang and Page-McCaw, 2014)

      Ectopic expression of wgUAS.cHa.Tag:HA under the control of Scer\GAL4btl.PS (in combination with a Gal80[ts] transgene to overcome embryonic and larval lethality) in spiracular branch (SB) growth zones 1 and 2, leads to proliferation of the SB cells and expansion of SB growth zone 2. The wgUAS.cHa.Tag:HA expressing SB zone 1 cells resemble zone 2 cells; they do not undergo endoreplication as indicated by their small nuclei, but instead remain in an undifferentiated state.

      (Pitsouli and Perrimon, 2013)

      Expression of wgScer\UAS.cHa.T:Ivir\HA1 in post-mitotic eye cells under the control of Scer\GAL4GMR.PF results in smaller eyes that have a 'glossy' appearance. Analysis of the eye nipple arrays in these overexpression mutants reveals a catastrophic loss of nipples, with the remaining nanostructures randomly spaced with large gap areas.

      (Kryuchkov et al., 2011)

      Overexpression of wgScer\UAS.cHa.T:Ivir\HA1 using Scer\GAL4dpp.blk1 decreases the nuclear size of distal salivary gland cells. Thus, the normal difference in size between proximal and distal salivary gland cells is small in these animals.

      Very few nuclei incorporate BrdU in wgScer\UAS.cHa.T:Ivir\HA1, Scer\GAL4dpp.blk1 salivary glands.

      (Taniue et al., 2010)

      Expression of wgScer\UAS.cHa.T:Ivir\HA1 in the larvae under the control of Scer\GAL4Act5C.Switch.PR results in lethality. Both male and female life-span are reduced when Ras85DV12.Scer\UAS is expressed in adult flies.

      (Shen et al., 2009)

      Expression of wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4dome-PG14 blocks differentiation of hemocyte precursors in the lymph gland.

      Expression of wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4Antp-10 results in an increase in the number of cells of the posterior signalling center of the lymph gland.

      Expression of wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4lz-gal4 results in an increase in the number of crystal cells.

      (Sinenko et al., 2009)

      Expression of wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4elav.PH in clones induced by FLP/FRT mediated recombination (to activate the Scer\GAL4elav.PH driver) results in an increase in the number of type 1b boutons at muscle 13 compared to wild type.

      (Haussmann et al., 2008)

      Overexpression of wgScer\UAS.cHa.T:Ivir\HA1 starting from early larval stages driven by Scer\GAL4byn-Gal4 in combination with Scer\GAL80ts.αTub84B results in a marked expansion of the size of the hindgut proliferation zone in late-stage larvae and in newly eclosed adult flies. A similar effect can be achieved by expressing wgScer\UAS.cHa.T:Ivir\HA1 exclusively during the adult stage. At higher expression levels of wgScer\UAS.cHa.T:Ivir\HA1 (achieved by increasing the temperature for the experiments) the proportion of spindle cell zone cells in the posterior intestine is increased compared to wild-type. Overexpression of wgScer\UAS.cHa.T:Ivir\HA1 at somewhat lower temperatures results in a less extreme expansion of the hindgut proliferation zone and there is an apparent increase in round cell zone cells.

      (Takashima et al., 2008)

      Embryos derived from females homozygous for wgScer\UAS.cHa.T:Ivir\HA1 crossed to males homozygous for Scer\GAL4e22c have a naked cuticle phenotype.

      (Chao et al., 2003)

      When wgScer\UAS.cHa.T:Ivir\HA1 is driven by Scer\GAL4C380 or Scer\GAL4C164, a slight increase in the number of synaptic boutons is seen.

      (Packard et al., 2002)

      Expression of wgScer\UAS.cHa.T:Ivir\HA1 using Scer\GAL4e22c in wgl-17 embryos causes all ventral epidermal cells to secrete naked cuticle. wgl-17 embryos expressing wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4wg.PM show full rescue of the ventral cuticle pattern, although the embryos are slightly distorted as the dorsal pattern elements are not rescued.

      (Hays et al., 1997)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      Scer\GAL4ptc.PU, wgUAS.cHa.Tag:HA has visible | adult stage phenotype, non-enhanceable by CtBP[+]/CtBP87De-10

      (Worley et al., 2018)
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4ptc.PU, wgUAS.cHa.Tag:HA has visible | adult stage phenotype, non-suppressible by CtBP[+]/CtBP87De-10

      (Worley et al., 2018)
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Phenotype Manifest In
      NOT Enhanced by
      Statement
      Reference

      Scer\GAL4ptc.PU, wgUAS.cHa.Tag:HA has wing | ectopic phenotype, non-enhanceable by CtBP[+]/CtBP87De-10

      (Worley et al., 2018)
      Suppressed by
      NOT suppressed by
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of wgUAS.cHa.Tag:HA under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) neither in combination with egrUAS.cUa nor in CtBP87De-10/+ background leads to ectopic wings compared to controls.

      Restricted expression of wgUAS.cHa.Tag:HA to second but not third instar larvae (under the control of Scer\GAL4ptc.PU and tub-Gal80[ts]) leads to ectopic wing pouches in wing discs in CtBP87De-10/+ background compared to controls.

      (Worley et al., 2018)

      The increased number of intestinal progenitor cells in mitosis characteristic for adult flies expressing wgScer\UAS.cHa.T:Ivir\HA1 driven by Scer\GAL4esg-NP7397 (combined with tub-Gal80[ts] for temporal control) is suppressed by co-expression of RetGD45.

      (Perea et al., 2017)

      The reduction in total cell number per clone and reduction in number of Dl-positive intestinal stem cells per clone seen in MARCM clones in the intestine of lin-28Δ1 adults is not rescued by expression of wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4tub.PU.

      (Chen et al., 2015)

      Co-expression of Mmp2Scer\UAS.cPa has no effect on the increase in stalk cell numbers seen in females expressing wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4C587 (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit expression to the adult stage).

      (Wang and Page-McCaw, 2014)

      Co-expression of sspScer\UAS.cTa and wgScer\UAS.cHa.T:Ivir\HA1 using Scer\GAL4dpp.blk1 suppresses the heterogeneity of salivary gland nuclear size seen when sspScer\UAS.cTa is expressed alone.

      (Taniue et al., 2010)

      The naked cuticle phenotype of embryos derived from females homozygous for wgScer\UAS.cHa.T:Ivir\HA1 crossed to males homozygous for Scer\GAL4e22c is not affected by eRF1unspecified or Elfunspecified.

      (Chao et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      wgUAS.cHa.Tag:HA/Scer\GAL4nSyb.PS rescues wg1/wgGBM

      (Huang et al., 2018)

      wgUAS.cHa.Tag:HA/Scer\GAL4futsch-C380 rescues wgl-12

      (Packard et al., 2002)

      wgUAS.cHa.Tag:HA/Scer\GAL4wg.PM rescues wgl-17

      (Hays et al., 1997)
      Comments

      wgScer\UAS.cHa.T:Ivir\HA1 completely rescues the neuromuscular junction expansion phenotype seen in wgl-12 animals, when driven by Scer\GAL4C380.

      (Packard et al., 2002)
      Images (0)
      Mutant
      Wild-type
      Stocks (3)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Exists in clones, not reported as being germ-line transmitted.

      (Hays et al., 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      wgScer\UAS.cHa.T:Ivir\HA1
      wgUAS.cHa.T:HA1
      wgUAS.cHa.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (41)