Allele Dmel\ple^+t8

General Information
Symbol	Dmel\ple^+t8	Species	D. melanogaster
Name		FlyBase ID	FBal0065709
Feature type	allele	Associated gene	Dmel\ple

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - genomic fragment
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - genomic fragment (Neckameyer and White, 1993)
Mutations Mapped to the Genome

Type Location Additional Notes References rescue fragment 3L:6,703,694..6,711,809 comment=The position of the restriction fragment on the reference sequence was inferred by a FlyBase curator. evidence=experimental linked_to=SmaI-EcoRI_rfrag (Neckameyer and White, 1993)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference 1.2kb of ple 5' upstream sequences fused to 7kb of ple genomic sequence containing the complete coding region, including all intronic sequences. (Neckameyer et al., 2001) Construct: 8kb ple genomic SmaI-EcoRI fragment containing 1kb of 5' upstream and 1kb of 3' downstream sequences. (Neckameyer and White, 1993)
Carried in construct	P{ple⁺8} (Neckameyer and White, 1993, Neckameyer et al., 2001, Bang et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	wild-type (Neckameyer and White, 1993)
Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	ple^+t8 rescues ple⁴ (Bang et al., 2011)
Partially rescues	ple^+t8 partially rescues ple² (Neckameyer et al., 2001, Neckameyer and White, 1993) ple^+t8 partially rescues ple⁴ (Neckameyer et al., 2001, Neckameyer and White, 1993)
Comments	The abnormal temperature-preference behaviour of ple[4] flies (reduced avoidance index against low temperatures compared to wild type) is rescued by ple[+t8]. (Bang et al., 2011) ple^+t8 rescues ple² and ple⁴ mutants 100% through the larval stage, but adult rescue is less than 50%. Those adults that do eclose are paler and weaker than their wild-type siblings. (Neckameyer et al., 2001)
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Fragment contains the necessary elements for correct temporal and spatial expression of the gene but may not contain all the sequences essential for quantitative expression. (Neckameyer and White, 1993)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	ple^+t8
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Bang et al., 2011, PLoS Genet. 7(3): e1001346 Dopamine signalling in mushroom bodies regulates temperature-preference behaviour in Drosophila. [FBrf0213392] Neckameyer et al., 2001, J. Neurobiol. 47(4): 280--294 Dopamine and sensory tissue development in Drosophila melanogaster. [FBrf0135852] Neckameyer and White, 1993, J. Neurogenet. 8(4): 189--199 Drosophila tyrosine hydroxylase is encoded by the pale locus. [FBrf0058913]

Allele Dmel\ple+t8

Allele Dmel\ple^+t8