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General Information
Symbol
Dmel\btl::Egfrλ.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0065871
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-λtop, UAS-EGFRλtop, UAS-λ-TOP, UAS-EGFRACT, UAS-dEGFRλ, UAS-λtop4.2, UAS-λDER, λ-TOP, EGFRλTop, EGFRAct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of a constitutively active form of Egfr that consists of a signal peptide, the dimerization domain of the bacteriophage λ cI repressor (included to induce constitutive dimerization of the encoded protein), a 0.15kb of btl sequence that includes the transmembrane domain and a 1.9kb fragment that contains the 3' end of Egfr (this fragment includes the intracellular domain).
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
actin filament & follicle cell
embryonic trachea & cortical actin cytoskeleton, with Scer\GAL4btl.PS
mesothoracic tergum & macrochaeta | ectopic, with Scer\GAL4sca-C253
wing margin & axon tract, with Scer\GAL4Bx-MS1096
Detailed Description
Statement
Reference
Expressing btl::Egfrλ.UAS under the control of Scer\GAL4ptc.PU leads to a significant decrease in size of the wing blade domain between L3 and L4 veins.
The expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4109-30, in combination with gal80[ts] to restrict expression to adulthood, leads to defects in follicle cell differentiation and in basal stalk formation, as compared to controls. The expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4upd1.PU leads to no obvious defects in either the differentiation of follicle cells or the basal stalk formation, as compared to controls.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4esg-NP5130 in intestinal stem cell (ISC) somatic flip-out clones (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut results in clone overgrowth and increased ISC division.
In wild-type third instar larvae, subperineurial glial cells (SPGs) form autocellular contacts with pronounced septate junction, the length of these autocellular contacts is significantly increased upon expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of either Scer\GAL4repo.PL or Scer\GAL4moody.SPG (in SPGs).
Expressing btl::Egfrλ.UAS under the control of Scer\GAL4repo results in increased glial cell numbers in the eye discs and optic stalks of third instar larvae.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CY2 does not affect the formation of cytoophidia (CTPsyn filaments) in egg chambers but results in a defective eggshell.
Follicle cells expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CY2 have a wild-type columnar architecture.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CY2 results in expansion of the eggshell operculum.
Mutant wing disc clones expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4tub.PU do not show neoplastic transformation.
Flies expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL471B have extra wing veins.
Clones in the eye disc expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4Act.PU produces ectopic photoreceptor clusters but does not result in apical constriction of the cells.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4C855a results in strong overproliferation of the inner optic anlagen neuroepithelia.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD in the posterior signalling center (PSC) under the control of either Scer\GAL4Hml.Δ leads to an increase in the number of circulating lamellocytes.
Follicle cell clones expressing btl::EgfrScer\UAS.T:λ\cI-DD generated using the MARCM system under the control of Scer\GAL4Act5C.PU do not overproliferate.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4EQ1 results in a dorsalised egg shell phenotype.
Activation of Egfr in Scer\GAL4bs-1348/+;btl::EgfrScer\UAS.T:lambda\cI-DD/+ flies increases sleep.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD driven by Scer\GAL4esg-NP7397 in adult midgut progenitor cells results in their overproliferation compared with control third instar larval midguts.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4repo.PU induces 5-10 fold excess glia in the larval brain and late pupal lethality. Glial clones expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4repo.PU contain two-fold more cells than wild type.
Expression of btl::Egfrλ.UAS in larvae under the control of Scer\GAL4repo results in a thickened optic stalk due to increased numbers of glial cells; glial cell numbers are significantly increased in the eye imaginal disc at all stages of eye disc development; there is also overmigration of glial cells along the Bolwig nerve, compared to controls.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD in clones in the eye disc under the control of Scer\GAL4unspecified results in ectopic photoreceptor differentiation.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CY2 completely eliminates the dorsal appendages of the eggshell and generates an operculum-like material at the anterior of the eggshell.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CY2 results in a dorsalized eggshell with no dorsal appendages.
Embryos that express btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4btl.PS show delayed tracheal extension in 35% of dorsal branches. The cells in these branches show rearrangements and do not intercalate as evidenced by the lack of autocellular adherens junctions. The tracheal tubules of these embryos show an accumulation of cortical actin compared to controls.
Wings of flies that express btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4GMR.PF have extra wing vein material.
Somatic clones of Scer\GAL4Act5C.PP cells in the eye disc in animals carrying btl::EgfrScer\UAS.T:λ\cI-DD cause ectopic photoreceptor differentiation.
btl::EgfrScer\UAS.T:λ\cI-DD; Scer\GAL4EQ1 mothers lay eggs with extra dorsal appendage material. The resulting embryos are doralized - with cuticles lacking denticle belts.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4bi-md653 results in a reasonably strong haltere-to-wing transformation as the capitellum of the halteres produce between 15 and 23 wing margin-type bristles.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4GR1 results in the basal actin fibers of the follicle cells appearing sparser and more disorganised than in wild type.
btl::EgfrScer\UAS.T:λ\cI-DD somatic clones in the dorsal air sac primordium are larger than wild-type clones, forming bulging outgrowths, but these clones are able to populate the priordium tip. These cells do not show any increase in lamelopodia formation compared to wild-type cells and their basal side remains inactive.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD, driven by Scer\GAL4sca-C253, leads to expression of ectopic bristles on the notum.
Targeted expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4MS1075 induces a number of axon guidance defects. Axons from the wing margin sensilla can also tangle and perforate the epithelial surface. At early stages of sensory axon extension, numerous ectopic contacts between axon and epithelium are seen. These ectopic contacts are clearly reduced by a 50% gene dosage reduction of Egfr.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4hs.PB using two 1 hour 37oC heat shocks at 14 hours after puparium formation (APF) and at 18 hours APF almost completely blocks the "late-stage" cell death which is normally seen in pupal retinas between 26-36 hours APF. However, "early-stage" cell death (which occurs between 18-24 hours APF) seems to be unaffected in these retinas.
Ganglionic branch 1s turn prematurely before reaching the midline in btl::EgfrScer\UAS.T:λ\cI-DD; Scer\GAL4bs-23.26 embryos.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4kni.L2 has no effect on the development of the L2 wing vein or neighbouring intervein cells.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4repo in embryos does not disrupt peripheral glial or neuronal development compared to wild-type embryos.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4ptc-559.1 results in abnormal patterning of the tarsal segments, but the rest of the leg is normal.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4sca-537.4 or Scer\GAL4Dll-md23 has no detectable effect on bract formation.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4Act5C.PP in clones results in an increase in cell size compared to controls.
Clones in the prospective notum in the wing disc expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4αTub84B.PZ intermix freely with surrounding cells, as shown by the "wiggly" borders of the clone. Clones in the prospective wing hinge expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4αTub84B.PZ sometimes form irregular patches (indicating mixing with surrounding cells) and sometimes form circular patches (indicating no mixing with surrounding cells).
Border cell migration is affected when btl::EgfrScer\UAS.T:λ\cI-DD is expressed under the control of either Scer\GAL4slbo.2.6; no border cell migration is seen in most stage 10 egg chambers.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4twi.PG results in an increase in the number of eve-positive mesodermal cells in the embryo.
Females expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CU1 at 22oC lay some wild-type eggs, but others have excess chorion blebs, enlarged dorsal appendages or other features suggesting dorsalisation. At higher temperatures, fewer wild-type eggs are laid and none hatch.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CY2 results in the formation of ectopic wing vein material at multiple sites on the wing. Females expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CY2 produce strongly dorsalised eggs with no dorsal appendages.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD driven by Scer\GAL4bi-omb-Gal4 causes reduction of the wing and transformation of the ventral pleura to notum. The wing is reduced to a stump covered with sensilla characteristic of the proximal wing (hinge) region. btl::EgfrScer\UAS.T:λ\cI-DD clones (driven by Scer\GAL4bi-omb-Gal4) in the adult wing form outgrowths or vesicles that differentiate structures lacking wing characteristics and that sometimes include bristles.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4T155 results in eggs having more than two dorsal appendages, and the resulting embryos are dorsalised. Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4E4 results in loss of posterior embryonic segments.
Eggs derived from females expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4T155 are dorsalised.
When under the control of Scer\GAL4CY2 a weak extra-vein phenotype is seen.
When expression is driven by Scer\GAL4T155 or Scer\GAL4dpp.blk1 the pleura is transformed to tergite and sternite identities.
When expression is driven by Scer\GAL4Bx-MS1096 the wings are small and bloated with an excess of vein material.
btl::EgfrScer\UAS.T:λ\cI-DD driven by Scer\GAL4ptc-559.1 produces adults with wings in which long stretches of the proximal ends of wing veins L3 and L4 are juxtaposed.
Embryos expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4twi.PG show a marked overproduction of eve-positive mesodermal founder cells.
When expression is driven by Scer\GAL4αTub84B.PP in the developing optic lamina, many cells destined for apoptosis undergo neuronal differentiation.
Embryos expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4twi.PG develop an increased number of eve-expressing mesodermal progenitors compared to wild-type.
When heat shock is driven by a heat inducible Scer\GAL4 for 1 hour in young pupae, programmed cell death in the retina is blocked, causing ectopic secondary and tertiary pigment cells.
Females expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4BY2 produce eggs with abnormal chorion patterns. Females expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4HS1 produce severely dorsalised eggs. Embryos expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4GQ2 have broader denticle belts than normal which are extended to the posterior and contain more denticles than normal. Adults expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4HV1 have extra wing vein material between wing veins 2 and 3.
Some ommatidia are enlarged and irregular in flies expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4T155. Females expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4T155 produce eggs with a mass of dorsal appendage material around the anterior circumference of the egg. The ectopic dorsal appendage material is still produced if the females are also homozygous for grk2E12. Females expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL455B produce mildly dorsalised eggs that have an increase in the dorsal midline space between the appendages, or ectopic dorsal appendage material in 43% of cases. Embryos derived from these eggs have dorsalised cuticle in the anterior region and appear normal in the posterior region. Extra wing veins are produced when btl::EgfrScer\UAS.T:λ\cI-DD is expressed under the control of Scer\GAL4CY2. Females expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CY2 produce eggs with no dorsal appendages. The entire anterior surface of the egg is covered in operculum-like material. Embryos derived from these eggs have strongly dorsalised cuticles. Females expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4E4 produce eggs that appear wild-type. Embryos derived from these eggs have dorsalised cuticle in the posterior region. Embryos derived from females expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CU1 have strongly dorsalised cuticles.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
btl::Egfrλ.UAS has actin filament bundle & follicle cell phenotype, enhanceable by Scer\GAL4GR1, btl::Egfrλ.UAS
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Scer\GAL4CU1, btl::Egfrλ.UAS has chorion phenotype, non-suppressible by Sk09312
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
The co-expression of groAA.Scer\UAS.P\T partially suppresses the basal stalk defects induced by the expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4109-30.
The overgrowth and increased mitotic rate of intestinal stem cell somatic flip-out clones expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4esg-NP5130 (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut is suppressed by co-expression of either cicScer\UAS.T:Ivir\HA1 or cicΔC2.Scer\UAS.T:Ivir\HA1; the number of mitotic cells per adult midgut is even lower than in wild-type.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD does not suppress the photoreceptor differentiation defects seen in Vps43B1 mutant clones, but induces premature photoreceptor differentiation in the wild type cells surrounding the clones. The increase in cell death seen in Vps43B1 mutant clones is not suppressed by btl::EgfrScer\UAS.T:λ\cI-DD.
Larvae co-expressing btl::Egfrλ.UAS alongside ifJF02695 under the control of Scer\GAL4repo show milder defects in eye disc glial cell numbers than either single expression condition.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4tub.PU in l(2)gl4 mutant wing disc mutant clones results in overproliferation and neoplastic transformation in the proximal domain. Clones in the distal domain are eliminated. However at an elevated growth temperature (29[o]C) distal neoplasia is also seen.
Co-expression of btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX under the control of Scer\GAL4repo.PU induces glial overgrowth in third instar larval brains. Expression of RIOK2GD7407 suppresses the glial overgrowth seen when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. There are few excess glia and remaining glial cells show abnormal development. The resulting brains are smaller in size than controls, due to a reduction in both glial and neuronal cells. Expression of RIOK2NIG.11859R suppresses the glial overgrowth seen when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. There are few excess glia and remaining glial cells show abnormal development. Expression of RIOK2KK101850 suppresses the glial overgrowth seen when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. There are few excess glia and remaining glial cells show abnormal development. Expression of RIOK1GD8541 suppresses the increase in brain size and glial overgrowth seen when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. There are few excess glia and remaining glial cells show abnormal development. The resulting brains are smaller in size than controls, due to a reduction in both glial and neuronal cells. Expression of RIOK1KK100810 suppresses the increase in brain size and glial overgrowth seen when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. There are few excess glia and remaining glial cells show abnormal development. The resulting brains are smaller in size than controls, due to a reduction in both glial and neuronal cells.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4Act5C.PU in flwFP41 mutant follicle cell clones results in overproliferation in anterior follicle cells but not in lateral follicle cells. The amount of cell overproliferation in posterior follicle cells is increased compared to flwFP41 clones alone.
Expression of Cbl::drkCblL-RING.drk-SH2.Scer\UAS.T:Zzzz\FLAG suppresses the dorsalised egg shell phenotype observed in animals expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4EQ1. Expression of CblL.RING.PR.Scer\UAS.T:Zzzz\FLAG fails to suppress the dorsalised egg shell phenotype observed in animals expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4EQ1. Expression of CblL.Scer\UAS suppresses the dorsalised egg shell phenotype observed in animals expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4EQ1.
Co-expression of btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX under the control of Scer\GAL4repo.PU induces progressive accumulation of ~50 fold excess glia in third instar larvae. The excess glia emerge in early larval stages and accumulate over 5-7 days. They severely disrupt the normal cellular architecture of the larval brain, lose normal stellate glial morphologies and generate multilayered aggregations of abnormal glia throughout the brain. The brains of larvae expressing btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX under the control of Scer\GAL4repo.PU also display abnormal polyploid glia. When brain fragments from larvae co-expressing btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX under the control of Scer\GAL4repo.PU are transplanted into young wild type adult flies, the mutant glia survive and proliferate into massive tumours that fill the host abdomen, often causing premature death. Tumours are composed of small glial cells with little cytoplasm. Tumours also contain trachea embedded throughout their mass. The leading edge of the tumor harbours individual cells invading nearby tissues, such as the ovary. However some tissues, such as the gut, do not contain metastases. btl::EgfrScer\UAS.T:λ\cI-DD Pten117 double mutant clones generated under the control of Scer\GAL4repo.PU are overgrown and invasive. Cells from mutant clones appear to invade the brain, typically following fibre tracts, and sometimes inducing the formation of trachea. Mutant cells often penetrate deep into the brain. Smaller clones are commonly seen that are composed of relatively differentiated, enlarged glia with diffusely invasive projections. btl::EgfrScer\UAS.T:λ\cI-DD Pten117 double mutant clones generated using eyFLP under the control of Scer\GAL4repo.PU contain more excess glial cells than in Pten117 alone. The clones are composed of 10s of glial cells in third instar larvae, becoming large invasive tumors composed of hundreds to thousands of cells in adults. Expression of PtenScer\UAS.cUa strongly suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. Expression of Ras85DN17.Scer\UAS moderately suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. Expression of dapScer\UAS.cdNa completely suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. The gross neural morphogenesis defects are also suppressed. Expression of RbfScer\UAS.cDa suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. The gross neural morphogenesis defects are also suppressed. Expression of pntGD1513 completely suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. One copy of stg01235 partially suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. Expression of stgScer\UAS.cNa enhances the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. Expression of Akt1GD1361 strongly suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. Expression of TorTED.Scer\UAS partially suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. Sin1e03756/Df(2R)BSC11 almost completely suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. Expression of foxoTM.Scer\UAS partially suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. Expression of raptorGD5138 significantly suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. Expression of S6kGD6646 significantly suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. Expression of eIF-4EGD1432 significantly suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. Expression of ThorGD12533 significantly suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. Expression of Tsc1GD11836 enhances the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. One copy of dmP0 suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. The late larval lethality is also partially suppressed. Expression of MaxGD4743 strongly suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. Cdk43/Cdk4k06503 completely suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. Co-expression of btl::EgfrScer\UAS.T:λ\cI-DD and dmScer\UAS.cZa under the control of Scer\GAL4repo.PU induces glial neoplasia in third instar larval brains. These glia have very compact cell bodies relative to wild type glia. Co-expression of btl::EgfrScer\UAS.T:λ\cI-DD, Cdk4Scer\UAS.cMa and CycDScer\UAS.cDa induces excess glia in third instar larval brains. Co-expression of btl::EgfrScer\UAS.T:λ\cI-DD and RbfGD4484 induces excess glia in third instar larval brains. Expression of RbfGD4484 enhances the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. These animals display exacerbated glial neoplasia, with a substantial increase in small anaplastic-like glia throughout the CNS.
Addition of Pi3K92EUAS.cUa to larvae expressing btl::Egfrλ.UAS under the control of Scer\GAL4repo enhances the phenotype of increased glial migration along the Bolwig nerve and reduces the number of excess glial cells in the eye disc.
The loss of photoreceptor cells seen in homozygous mopT612 clones in the eye disc is not rescued by expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4unspecified. Co-expression of mopScer\UAS.T:Zzzz\FLAG and btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4ap-md544 results in overgrowth of the dorsal compartment of the wing disc.
Expression of either GugScer\UAS.cCa or aopScer\UAS.cRa have very little effect on the extra wing vein material produced in flies that overexpress btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4GMR.PF. However, this wing vein phenotype is suppressed when GugScer\UAS.cCa and aopScer\UAS.cRa are both expressed along with btl::EgfrScer\UAS.T:λ\cI-DD (all under the control of Scer\GAL4GMR.PF).
The ectopic photoreceptor differentiation caused by somatic clones of Scer\GAL4Act5C.PP cells in the eye disc in animals carrying btl::EgfrScer\UAS.T:λ\cI-DD is not suppressed by raspT392/raspT802.
The formation of extra dorsal appendage material in eggs laid by btl::EgfrScer\UAS.T:λ\cI-DD; Scer\GAL4EQ1 mothers is suppressed if those mothers also carry CblL.Scer\UAS. The resulting eggs are mildly ventralized. It also largely suppresses the dorsalization of the resulting embryos: extensive denticle belts are formed. Only weak suppression of extra dorsal appendage material is seen with CblS.Scer\UAS. CblL.Scer\UAS suppresses wing defects seen in btl::EgfrScer\UAS.T:λ\cI-DD; Scer\GAL4EQ1 adults. In contrast, CblS.Scer\UAS does not rescue this phenotype.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4bi-md653 in an argosunspecified/+ background results in an enhancement of the haltere-to-wing transformation seen when btl::EgfrScer\UAS.T:λ\cI-DD is expressed in a wild-type background. Scer\GAL4bi-md653>btl::EgfrScer\UAS.T:λ\cI-DD; argosunspecified/+ flies produce an average 23.3 wing margin-type bristles on the capitellum of the haltere compared to 16.8 bristles produced by Scer\GAL4bi-md653>btl::EgfrScer\UAS.T:λ\cI-DD flies. Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4bi-md653 in a Ubx1/+ background results in a marginal reduction in the number of wing margin-type bristles seen on the capitellum in comparison to flies that express btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4bi-md653 in a wild-type background.
When ActnRpS27A.PD is expressed in a Scer\GAL4GR1>btl::EgfrScer\UAS.T:λ\cI-DD background, all basal actin fibers are of follicle cells are transformed into a single branched or unbranched F-actin bundle.
Coexpression of btl::EgfrScer\UAS.T:λ\cI-DD with NScer\UAS.icd, HLHm7Scer\UAS.cdCa and groScer\UAS.cAa, under the control of Scer\GAL4sca-C253, leads to suppression of the bristle-loss phenotypes seen when NScer\UAS.icd, HLHm7Scer\UAS.cdCa and groScer\UAS.cAa are expressed alone. In contrast coexpression of btl::EgfrScer\UAS.T:λ\cI-DD with groAA.Scer\UAS, also under the control of Scer\GAL4sca-C253, is unable to suppress the bristle-loss phenotype seen when groAA.Scer\UAS is expressed alone.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4twi.PG is not able to rescue either early contact between the mesoderm and ectoderm or symmetric flattening of the mesodermal tube in the early stages of mesoderm morphogenesis in htlAB42 embryos.
Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4salm-459.2 completely rescues the formation of oenocytes in abd-AM1 mutant embryos.
The proportion of shgk03401 embryos showing the severe ventral cuticle phenotype is enhanced by expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4da.G32.
The addition of btl::EgfrScer\UAS.T:λ\cI-DD has no effect on the N55e11 or Df(1)svr Malpighian tubule phenotypes, including the failure of late cell divisions in the tubules.
The dorsal appendage phenotype produced by females expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CY2 is partially suppressed if the females are also homozygous for ShcBG or Shc111-40; dorsal appendage material is produced around the anterior circumference of the egg. The ectopic wing vein phenotype caused by expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CY2 is completely suppressed if the flies are also homozygous for ShcBG or Shc111-40. The dorsalised egg phenotype produced by females expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CY2 is partially suppressed if the females are also homozygous for ShcBG or Shc111-40; dorsal appendage material is produced around the anterior circumference of the egg.
When piphs.PS is expressed in females expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4ET3, ventralised embryos are produced inside dorsalised egg shells.
The ventralised phenotype of eggs derived from females expressing kek1Scer\UAS.cGa under the control of Scer\GAL4T155 is overridden if the females are also coexpressing btl::EgfrScer\UAS.T:λ\cI-DD; the resulting eggs are dorsalised.
When expression is driven by Scer\GAL4Bx-MS1096 in combination with styScer\UAS.cHa, the wing size of the btl::EgfrScer\UAS.T:λ\cI-DD is largely normal and the wing veins, though still slightly thickened compared to wild type, are largely restored.
The lack of eve-positive mesodermal cells seen in htlAB42 and stumpsYY202 embryos is partially rescued by btl::EgfrScer\UAS.T:λ\cI-DD expressed under the control of Scer\GAL4twi.PG.
Females expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4CY2 produce eggs with extremely dorsalised eggshells. This phenotype is not altered if the females are also carrying vas6356-001/Df(2L)b87e25.
Xenogenetic Interactions
Statement
Reference
Mesodermal expression of constitutively active btl::EgfrScer\UAS.T:λ\cI-DD in sli2 mutants (under the control of Scer\GAL4Mef2.PR) produces many small adhesion sites between midline-crossing ventral longitudinal muscles. This ectopic attachment phenotype is vg-specific as interfering with vg function through coexpression of sdΔ88-159.Scer\UAS results in fewer of these adhesion sites, whereas these adhesion sites become much bigger with increased vg expression (through vg2.Scer\UAS).
Complementation and Rescue Data
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Synonyms and Secondary IDs (14)
Reported As
Symbol Synonym
EGFRλtop[4.2]
btl::Egfr::λ\cIUAS.cQa
btl::EgfrScer\UAS.T:λ\cI-DD
btl::EgfrScer\UAS.T:λ\cI
btl::Egfrλ.UAS
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    References (123)