FlyBase Allele Report: Dmel\bam[hs.PO]

General Information

Symbol

Dmel\bam^hs.PO

Species

D. melanogaster

Name

heat shock construct of Ohlstein

FlyBase ID

FBal0065895

Feature type

allele

Associated gene

Dmel\bam

Associated Insertion(s)

Carried in Construct

P{hs-bam.O}

Also Known As

hs-bam, P{HS-Bam}

Key Links

Transgenic product class

genomic_DNA

(Ohlstein and McKearin, 1997)

wild_type

(Ohlstein and McKearin, 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Ohlstein and McKearin, 1997)

wild_type

(Ohlstein and McKearin, 1997)

Mutagen

in vitro construct

(Ohlstein and McKearin, 1997)

Progenitor genotype

Carried in construct

P{hs-bam.O}

Cytology

Description

A genomic fragment containing most of the bam transcription unit (including the entire open reading frame) and approximately 200bp 3' flanking sequences is expressed under the control of a heat shock promoter.

(Ohlstein and McKearin, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

bam

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage | heat sensitive

(Lim et al., 2015)

decreased cell number | heat sensitive

(Liu et al., 2015, Insco et al., 2009)

decreased cell number | heat sensitive, with bam^Δ86

(Liu et al., 2015)

radiation resistant | adult stage | female

(Ma et al., 2016)

Phenotype Manifest In

cyst progenitor cell | male limited | heat sensitive

(Sheng et al., 2009)

female germline stem cell

(Xie and Spradling, 2000)

female germline stem cell | heat sensitive

(Liu et al., 2015, Kai and Spradling, 2003)

female germline stem cell | heat sensitive, with bam^Δ86

(Liu et al., 2015)

germarium

(Ohlstein and McKearin, 1997)

germarium & germ cell

(Kirilly et al., 2005)

germarium | heat sensitive

(Liu et al., 2015)

germarium | heat sensitive, with bam^Δ86

(Liu et al., 2015)

germline cell

(Lavoie et al., 1999)

germline cell | female | larval stage | heat sensitive

(Gilboa and Lehmann, 2004)

germline cyst | ectopic | heat sensitive

(Gilboa and Lehmann, 2004)

germline cyst | heat sensitive

(Kai and Spradling, 2004)

male germline stem cell | adult stage | heat sensitive

(Lim et al., 2015)

male germline stem cell | heat sensitive

(Sheng et al., 2009, Kawase et al., 2004)

ovary

(Ohlstein and McKearin, 1997)

ovary | larval stage | heat sensitive

(Kai and Spradling, 2004)

spermatogonial cyst | heat sensitive

(Insco et al., 2009)

spermatogonium | heat sensitive

(Sheng et al., 2009)

Detailed Description

Statement

Reference

Heat-shock induced expression of bam^hs.PO prevents the accumulation of cystoblast-like cells in adult female germaria following X-ray irradiation (to introduce DNA damage).

(Ma et al., 2016)

Expression of bam^hs.PO via heat shock treatment in adults leads to male germline stem cells differentiating and moving away from the niche, depleting germline stem cells. When heat shocks are ceased, germline stem cell number partially recovers due to spermatogonial dedifferentiation.

(Lim et al., 2015)

Expression of bam^hs.PO induced via heat shock results in increased germline stem cell differentiation, as evidenced by a lack of germline stem cells 1 day after heat shock, and germaria exhibiting only fusome-containing cysts within the niche, in contrast to controls. At 7 days after heat shock, a subset of germaria exhibit restoration of germline stem cells.

Expression of bam^hs.PO induced via heat shock in a bam^Δ86/bam^Δ86 background results in increased germline stem cell differentiation, as evidenced by a lack of germline stem cells 24 h after heat shock, and germaria exhibiting only fusome-containing cysts within the niche, and very few spectrosome-containing cells; however at 48 h after heat shock, a majority of germaria show some single spectrosome-containing cells.

(Liu et al., 2015)

One day after expressing bam^hs.PO in females using heat shock, germ cells in the ovary contain fusomes, consistent with germline stem cell differentiation.

(Barton et al., 2013)

bam^hs.PO males treated with heat-shock show premature differentiation of germline cysts at the 8-cell stage in 18% of cysts. bam^hs.PO and wild-type flies raised without heat-shock produce no 8-cell cysts.

(Insco et al., 2009)

Testes from bam^hs.PO flies progressively lose germline stem cells (GSCs) with increasing numbers of heat-shocks. There is no increase in cell death under these conditions, suggesting these GSCs differentiate rather than die. After 5 heat shocks, 53% of testes have no GSCs but spermatogonial cysts are retained - additional heat shocks impair adult viability and yield testes with few spermatogonia.

Ectopic bam^hs.PO expression does not alter the number of cyst progenitor cells (CPCs) or trigger their premature differentiation; nor is the number or appearance of hub cells affected. The nuclei of hub and early somatic cells also appear similar to controls. However, the fine extensions that CPCs normally extend toward the hub are replaced by broad areas of contact in bam^hs.PO testes - CPCs move in to fill the area surrounding the hub upon GSC loss.

After 1 day of recovery after a 5x heat shock regime, the average number of GSCs decreases in bam^hs.PO testes and only 10% of testes contain any GSCs. However, after 4 days of recovery, most testes contain both GSCs and spermatogonial cysts adjacent to the hub, and by 6 days, most testes are indistinguishable from wild type.

Administering 10 heat shocks to bam^hs.PO animals reduces the number of testes containing spermatogonia to 48%, and only 36% regain GSCs upon withdrawal of heat shock. Administering 15 heat shocks depletes spermatogonia from all testes - none regain GSCs upon withdrawal of heat shock.

In contrast to controls, heat-shocked bam^hs.PO testes that are depleted of GSCs and then allowed to recover show multiple ring canal remnants in germ cells near, but not necessarily adjacent to, the hub (beginning at 2 days of recovery). These remnants must be products of spermatogonial breakdown.

Whereas control testes contain germ cells located within 3μm of the hub, induction of bam^hs.PO results in only 60% of testes containing germ cells within 3μm of the hub - the remaining 40% contain hubs entirely surrounded by somatic cells. Upon withdrawal of heat shock, germ cells return to the hub and all testes contain germ cells by 7 days of recovery.

51% of bam^hs.PO testes undergoing dedifferentiation (i.e. after heat shock) contain 4-cell spermatogonial cysts with fine actin-rich protrusions compared to only 8.3% in controls.

(Sheng et al., 2009)

In bam^hs.PO females 10 days after two 2 hr heat shock treatments, no germ-line stem cells or their descendant are present in germaria. Somatic stem cell fill the stem cell niche, producing a bag of follicle cells.

(Kirilly et al., 2005)

75% of larval ovaries in which bam^hs.PO is expressed using heat shock at the end of the first larval instar have many cysts, indicating premature differentiation of the primordial germ cells. Ovaries in which bam^hs.PO is expressed at the end of the second larval instar show differentiation of all primordial germ cells into cysts. Ovaries in which bam^hs.PO is expressed at the end of embryogenesis do not contain cysts.

(Gilboa and Lehmann, 2004)

When heat-shock is administered at the start of the second larval instar, 20 hours later, no single germ cells or cell pairs remain. Instead only 4 and 8-cell cysts are found in numbers that correspond closely to the starting number of single and transiently interconnected germ cell pairs, respectively. It seems that the differentiating germ cells revert to stem cells. The characteristic shape of the fusomes and appearance of ring canals in these larval cysts are indistinguishable from adult cysts. Over the next 20-30 hours of larval development, however, the cysts become unstable and begin to break down. By 50 hours after heat shock, only single cells are found in mutant ovaries. Apoptosis does not seem to be the cause of these phenotypes. The cysts appear to break into single cells by ring canal closure and fusome fragmentation. 50 hours after heat shock, ring canal remnants can be seen in the single germ cells. Adult females who have received heat treatment have normal numbers of wild type germ cell cysts. The environment surrounding the stem cells is indistinguishable from controls and constituted a normal niche. Five day old treated and control females lay normal eggs at about the same rate as controls. the reconstituted stem cells appear to have a normal half life.

(Kai and Spradling, 2004)

Two days after heat shock, the average number of germline stem cells per testis is reduced to 3.9 (compared to 9.3 in controls). A week after heat shock about 70% of the testes have no germline stem cells, with an average of 2 per testis, compared to 7.6 in controls.

(Kawase et al., 2004)

When animals are heatshocked at 37^oC for an hour, followed by an hour at 25^oC, and then another hour at 37^oC, the germ-line stem cells (GSCs) differentiate and exit the GSC niche within 24 hours. After 4 days, the youngest germ cells are in region 2b cysts, whereas after 7 days, the only germ cells remaining in the germarium are in a single stage-1 follicle. The terminal filament cells and cap cells (CpCs), remain stable for an extended period after the GSCs depart. Terminal filament cells change little for at least three weeks. Moreover CpCs appear normal even when all germ cells are gone 8-10 days after heat shock. However by 18 days after GSC loss, CpCs could no longer be detected. Inner-germarium sheath cells (IGSs) are much less stable, no IGSs are detected 8-9 days after ablation. The departure of germ cells and the death IGSs brings somatic stem cells (SSCs) increasingly closer to the GSC niche. Ectopic follicle cell progenitors divide within the vacated niche but IGSs do not.

(Kai and Spradling, 2003)

Over the course of several days after heat shock, bam^hs.PO germ cell stem cells (GSCs) are lost and all germ line cysts complete development and leave the germarium. Such germaria lose all inner germarium sheath cells (ISCs). In contrast terminal filament and cape cells do not change in the absence of germ cells. Somatic cells continue in their vicinity as in germaria that form in the presence of germ cells. Despite their presence near the GSC niche, these dividing somatic cells do not become GSCs.

(Xie and Spradling, 2000)

Overexpression of bam with P{hs-bam.O} causes germ cell loss.

(Lavoie et al., 1999)

Females carrying bam^hs.PO that are heat-shocked daily for 6 days have dramatically smaller ovaries than heat-shocked wild-type females. The ovarioles contain maturing egg chambers, but none are younger than stage 3 or 4. The germaria are shrunken. The germaria of females carrying bam^hs.PO that are 7 or more days post-heat shock appear to be completely devoid of germ cells, and are filled with a small number of squamous cells. Somatic stem cell populations are not affected by ectopic expression of bam^hs.PO. Ectopic expression of bam^hs.PO has no deleterious effects for male germline cells; heat-shocked males carrying bam^hs.PO are fertile and spermatogenesis appears normal.

(Ohlstein and McKearin, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

bam^hs.PO is a suppressor | partially of decreased cell number | oogenesis phenotype of sov^ML150/sov²

(Jankovics et al., 2018)

bam^hs.PO is a suppressor | partially of neoplasia | oogenesis phenotype of sov^ML150/sov²

(Jankovics et al., 2018)

bam^hs.PO is a suppressor | temperature conditional of increased cell number | female | adult stage phenotype of Psc^GD4481, Scer\GAL4^C587, Su(z)2^GD16388

(Li et al., 2016)

bam^hs.PO is a suppressor | temperature conditional of neoplasia | female | adult stage phenotype of Psc^GD4481, Scer\GAL4^C587, Su(z)2^GD16388

(Li et al., 2016)

Other

Statement

Reference

Scer\GAL4^C587, bam^Δ86, bam^hs.PO, dpp^JF01371 has decreased cell number | heat sensitive phenotype

(Liu et al., 2015)

Scer\GAL4^C587, bam^Δ86, bam^hs.PO, ptc^JF03223 has decreased cell number | heat sensitive phenotype

(Liu et al., 2015)

Scer\GAL4^C587, bam^hs.PO, dpp^JF01371 has decreased cell number | heat sensitive phenotype

(Liu et al., 2015)

bam^hs.PO, zpg^unspecified has decreased cell number | oogenesis phenotype

(Gilboa et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

bam^hs.PO has male germline stem cell | heat sensitive phenotype, enhanceable by Socs36E^UASp.cCa/Scer\GAL4^Act5C.PP

(Sheng et al., 2009)

Enhancer of

Statement

Reference

bam^hs.PO is an enhancer of germ cell & germarium phenotype of zpg^unspecified

(Gilboa et al., 2003)

NOT Enhancer of

Statement

Reference

bam^hs.PO is a non-enhancer of egg chamber phenotype of bru1^QB

(Parisi et al., 2001)

Suppressor of

Statement

Reference

bam^hs.PO is a suppressor | partially of female germline stem cell phenotype of sov^ML150/sov²

(Jankovics et al., 2018)

bam^hs.PO is a suppressor | partially of cystoblast phenotype of sov^ML150/sov²

(Jankovics et al., 2018)

bam^hs.PO is a suppressor | temperature conditional of germarium phenotype of Psc^GD4481, Scer\GAL4^C587, Su(z)2^GD16388

(Li et al., 2016)

bam^hs.PO is a suppressor of female germline stem cell | increased number phenotype of Scer\GAL4^{VP16.nanos.UTR}, lili^UASp.cDa

(Dolezal et al., 2015)

bam^hs.PO is a suppressor of male germline cell phenotype of mael^M391/Df(3L)79E-F

(Pek et al., 2009)

bam^hs.PO is a suppressor of spermatogonial cyst | increased number phenotype of Scer\GAL4^nanos.PG, mir-7^UAS.cLb

(Pek et al., 2009)

bam^hs.PO is a suppressor of egg chamber phenotype of twin^ry3

(Morris et al., 2005)

bam^hs.PO is a suppressor of oocyte phenotype of twin^ry3

(Morris et al., 2005)

bam^hs.PO is a suppressor of cystoblast phenotype of Scer\GAL4^{VP16.nanos.UTR}, tkv^Q253D.UASp

(Casanueva and Ferguson, 2004)

bam^hs.PO is a suppressor of egg chamber phenotype of Scer\GAL4^{VP16.nanos.UTR}, tkv^Q253D.UASp

(Casanueva and Ferguson, 2004)

bam^hs.PO is a suppressor of ovary phenotype of Scer\GAL4^{VP16.nanos.UTR}, tkv^Q253D.UASp

(Casanueva and Ferguson, 2004)

bam^hs.PO is a suppressor of female germline stem cell | ectopic phenotype of Scer\GAL4^{VP16.nanos.UTR}, tkv^Q253D.UASp

(Casanueva and Ferguson, 2004)

NOT Suppressor of

Statement

Reference

bam^hs.PO is a non-suppressor of egg chamber phenotype of bru1^QB

(Parisi et al., 2001)

bam^hs.PO is a non-suppressor of germline cell phenotype of bgcn¹

(Lavoie et al., 1999)

Other

Statement

Reference

Df(3R)ED6235/jus^iso7.8, bam^hs.PO has male germline stem cell | adult stage | heat sensitive phenotype

(Lim et al., 2015)

CG46339^UASp.Tag:HA, Df(3R)ED6235/jus^iso7.8, Scer\GAL4^upd1.PU, bam^hs.PO has male germline stem cell | adult stage | heat sensitive phenotype

(Lim et al., 2015)

Scer\GAL4^C587, bam^Δ86, bam^hs.PO, dpp^JF01371 has germarium | heat sensitive phenotype

(Liu et al., 2015)

Scer\GAL4^C587, bam^Δ86, bam^hs.PO, ptc^JF03223 has germarium | heat sensitive phenotype

(Liu et al., 2015)

Scer\GAL4^C587, bam^hs.PO, dpp^JF01371 has germarium | heat sensitive phenotype

(Liu et al., 2015)

Scer\GAL4^C587, bam^hs.PO, dpp^JF01371 has female germline stem cell | heat sensitive phenotype

(Liu et al., 2015)

Su(var)3-3^ΔN, bam^hs.PO has female germline cyst phenotype

(Eliazer et al., 2011)

bam^hs.PO, gbb⁴/gbb^D4 has follicle cell & germarium | conditional - heat sensitive phenotype

(Kirilly et al., 2005)

bam^hs.PO, gbb⁴/gbb^D4 has germarium | heat sensitive phenotype

(Kirilly et al., 2005)

bam^hs.PO, gbb^D20/gbb⁴ has follicle cell & germarium | conditional - heat sensitive phenotype

(Kirilly et al., 2005)

bam^hs.PO, gbb^D20/gbb⁴ has germarium | heat sensitive phenotype

(Kirilly et al., 2005)

Scer\GAL4^C587, bam^hs.PO, dpp^UAS.cNa has germline cyst | heat sensitive phenotype

(Kai and Spradling, 2004)

bam^hs.PO, zpg^unspecified has female germline stem cell phenotype

(Gilboa et al., 2003)

bam^hs.PO, zpg^unspecified has ovariole phenotype

(Gilboa et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of both Psc^GD4481 and Su(z)2^GD16388 under the control of Scer\GAL4^C587 (together with tub-Gal80[ts] to limit the time of expression to adulthood) results in tumorigenic accumulation of spectrosome containing germline stem cell-like (GSC-like) cells (which increase in number with the time of expression) in female germaria. Expression of bam^hs.PO (induced by 1hr heat-shock once a day for 5 consecutive days) induces differentiation of the tumor cells.

(Li et al., 2016)

sda^iso7.8/Df(3R)ED6235 mutants fail to undergo spermatogonial dedifferentiation and germline stem cell number recovery in the testicular niche after expression of bam^hs.PO via heat shock treatment.

Expression of sda^{Scer\UAS.P\T.T:Ivir\HA1}, but not sda^{ΔCAT.Scer\UAS.P\T.T:Ivir\HA1} or sda^{E-A.Scer\UAS.P\T.T:Ivir\HA1}, under the control of Scer\GAL4^upd1.PU, restores the ability of sda^iso7.8/Df(3R)ED6235 mutants to undergo spermatogonial dedifferentiation and recover germline stem cell number in the testicular niche after expression of bam^hs.PO via heat shock treatment.

(Lim et al., 2015)

Expression of dpp^JF01371 under the control of Scer\GAL4^C587 in flies expressing bam^hs.PO induced via heat shock results in germaria exhibiting complete loss of germline stem cells 1 day after heat shock, similar to those expressing bam^hs.PO alone; however, at 7 days after heat shock, the proportion of germaria that show restoration of germline stem cells is substantially lower than that of flies expressing bam^hs.PO alone.

Expression of dpp^JF01371 or ptc^JF03223, but not ci^GD1403, under the control of Scer\GAL4^C587 in flies expressing bam^hs.PO induced via heat shock in a bam^Δ86/bam^Δ86 background suppresses the recovery of spectrosome-containing cells at 48 h after heat shock seen in flies expressing bam^hs.PO alone in a bam^Δ86/bam^Δ86 background.

(Liu et al., 2015)

Expression of bam^hs.PO does not fully suppress the apoptosis of mutant germline cells which is seen in ball² larval ovaries.

(Herzig et al., 2014)

Expression of CSN4^{Scer\UAS.P\T.T:Ivir\HA1} in the germline under the control of Scer\GAL4^nos.PU partially suppresses the germline stem cell (GSC) loss seen upon expression of bam^hs.PO. 65% of germaria retain GSCs, compared to 83-95% when bam^hs.PO is expressed alone.

(Pan et al., 2014)

Germ cells with fusomes are found in ovaries one day after heat shock in Ote^B279/Ote^B279 or Ote^PK/Ote^B279 females carrying bam^hs.PO. Three days after heat shock the mutant germaria are largely devoid of germ cells and the ovaries contain a very low number of egg chambers.

(Barton et al., 2013)

Expression of bam^hs.PO in Su(var)3-3^ΔN mutants results in the formation of multicellular cysts that contain branched fusomes indicating that Su(var)3-3^ΔN mutant germline cells can undergo differentiation and formation of multicellular cysts.

(Eliazer et al., 2011)

The differentiation defects seen in the testes of mael^M391/Df(3L)79E-F males are rescued by expression of bam^hs.PO.

The increase in the number of spermatogonial cysts per testis that is seen in males expressing mir-7^Scer\UAS.cLb under the control of Scer\GAL4^nos.PG is suppressed if they are also expressing bam^hs.PO.

(Pek et al., 2009)

bam^hs.PO testes recovering after heat shock that also express Socs36E^{Scer\UAS.P\T.cCa} via Scer\GAL4^Act5C.PP show a marked decrease in their ability to regain new germ line stem cells compared to recovering testes not expressing Socs36E^{Scer\UAS.P\T.cCa}.

(Sheng et al., 2009)

bam^hs.PO animals cultured at 18^oC and heat shocked as late stage pupae (37^oC, 4 times for 2 hours each with an 8-12 hour interval) produce adult females with agametic germaria. If these animals also carry the temperature sensitive allelic combination gbb⁴/gbb^D4 and are shifted to 29^oC (the non-permissive temperature) for 10 days, the majority of germaria disintegrate. Among remaining germaria, less than half contain follicle cells but all contain terminal filament cells. In identical experiments using gbb⁴/gbb^D20, no follicle cells remain, although the resulting germaria do contain terminal filament cells. In identical experiments using dpp^hr56/dpp^e90, the agametic germaria contain only slightly less follicle cells than controls (bam^hs.PO only).

(Kirilly et al., 2005)

The egg chamber defects seen in twin^ry3 hemizygotes at 25^oC are partly suppressed by expression of bam^hs.PO.

(Morris et al., 2005)

When bam^hs.PO is driven by heat shock in dpp^Scer\UAS.cNa, Scer\GAL4^C587 adults, cysts break down by a process of ring canal closure and fusome scission, de-differentiating. The multiple germ-line stem cells in each ovariole immediately and synchronously begin to differentiate after heat shock and soon generate 4-cell and 8-cell cysts. There are no single cells or cell pairs remaining at 20 h after heat shock. however by 30 h after heat shock (AHS) the cysts stop growing and start to beak down. By 40 h AHS closed ring canals and ring cell remnants are common. By 50 h AHS almost all germ cells are single.

(Kai and Spradling, 2004)

When bam^hs.PO, dpp^hr4/dpp^hr56, are heatshocked at 37^oC for an hour, followed by an hour at 25^oC, and then another hour at 37^oC then kept at the permissive temperature for 5 days and then shifted to 29^oC, follicle cell progenitors divide at the same rate as those cells without mutant dpp. When bam^hs.PO, hh^ts2, are heatshocked at 37^oC for an hour, followed by an hour at 25^oC, and then another hour at 37^oC then kept at the permissive temperature for 5 days and then shifted to 29^oC, follicle cell progenitors fail to divide. When bam^hs.PO, hh^hs.PI, are heatshocked at 37^oC for an hour, followed by an hour at 25^oC, and then another hour at 37^oC the follicle cell progenitors divide much more than those without hh^hs.PI.

(Kai and Spradling, 2003)

bam^Δ86 ovarian tumor cells transplanted into the dorsal mesoderm of stage 11-12 embryos from osk^unspecified mothers can repopulate the ovaries of the resulting adults, forming tumorous ovaries. The fertility of these flies can be rescued by heat shock if the transplanted cells carry bam^hs.PO.

(Niki and Mahowald, 2003)

Overexpression of bam with P{hs-bam.O} in a homozygous bgcn¹ mutant causes tumorous germ cells to develop, in which fusomes do not mature as normal (as for the bgcn¹ mutant alone). Germ line stem cells are not lost in the adults of these heat shocked P{hs-bam.O} homozygous bgcn¹ mutants, though they are eliminated in their bgcn¹/+ siblings.

(Lavoie et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

bam^hs.PO rescues bam¹/bam^Δ86

(Kim et al., 2017)

Partially rescues

bam^hs.PO partially rescues bam^Δ86

(Ohlstein and McKearin, 1997)

Comments

Heat-shock induced pulse expression of bam^hs.PO restores the production of functional sperm in bam¹/bam^Δ86 mutants.

(Kim et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

24636

P{hs-bam.O}18d, w¹¹¹⁸

24637

w¹¹¹⁸; P{hs-bam.O}11d/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

bam^hs.PO

Name Synonyms

heat shock construct of Ohlstein

(Ohlstein and McKearin, 1997)

Secondary FlyBase IDs

References (55)

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