FlyBase Allele Report: Dmel\Pka-C1[X4]

General Information

Symbol

Dmel\Pka-C1^X4

Species

D. melanogaster

Name

FlyBase ID

FBal0066002

Feature type

allele

Associated gene

Dmel\Pka-C1

Associated Insertion(s)

Carried in Construct

Also Known As

DCO^X4, DC0^X4

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm | cold sensitive

(Majercak et al., 1997)

abnormal memory | heat sensitive

(Li et al., 1996)

abnormal neurophysiology

(Renger et al., 2000)

abnormal neurophysiology | recessive

(Yao and Wu, 2001)

abnormal smell perception | heat sensitive

(Li et al., 1996)

abnormal thermotaxis (with Df(2L)γ15)

(Hong et al., 2008)

abnormal thermotaxis (with Pka-C1^MB581)

(Hong et al., 2008)

abnormal thermotaxis (with Pka-C1^Tw2)

(Hong et al., 2008)

lethal - all die before end of P-stage (with Pka-C1^B10)

(Li et al., 1996)

lethal - all die during third instar larval stage | heat sensitive

(Li et al., 1996)

partially lethal - majority die (with Pka-C1^B12)

(Li et al., 1996)

partially lethal - majority die (with Pka-C1^D30)

(Li et al., 1996)

partially lethal - majority die | heat sensitive (with Df(2L)γ15)

(Li et al., 1996)

partially lethal - majority die | heat sensitive (with Pka-C1^A13)

(Li et al., 1996)

partially lethal - majority die | heat sensitive (with Pka-C1^B3)

(Li et al., 1996)

partially lethal - majority die | heat sensitive (with Pka-C1^C4)

(Li et al., 1996)

partially lethal - majority die | heat sensitive (with Pka-C1^H3)

(Li et al., 1996)

partially lethal - majority live | heat sensitive (with Pka-C1^G9)

(Li et al., 1996)

partially lethal - majority live | heat sensitive (with Pka-C1^H2)

(Li et al., 1996)

viable

(Majercak et al., 1997)

Phenotype Manifest In

neuromuscular junction

(Renger et al., 2000)

neuron

(Yao and Wu, 2001)

Detailed Description

Statement

Reference

Pka-C1^X4/Pka-C1^Tw2 mutant flies show an inability to find a preferred temperature, with temperature preference spread across a wide gradient.

Pka-C1^X4/Df(2L)γ15 mutant flies show an inability to find a preferred temperature, with temperature preference spread across a wide gradient.

Pka-C1^X4/Pka-C1^MB581 mutant flies show an inability to find a preferred temperature, with temperature preference spread across a wide gradient.

(Hong et al., 2008)

The firing frequency is significantly increased in adaptive and tonic Pka-C1^X4 neurons. The action potential amplitude is significantly decreased in adaptive but slightly reduced in tonic neurons in Pka-C1^X4 mutant neurons. Pka-C1^X4 mutant neurons display erratic firing rates during current injection.

When Pka-C1^X4 mutant neurons are simulated by step current injections, the firing pattern, in particular the number of spikes in each spike train, varied to a much greater extent than that in wild-type cells.

(Yao and Wu, 2001)

As in wild-type, there is little variability in the time to peak of evoked ejcs in mutant boutons. There is a reduction in excitatory junctional current amplitude at the neuromuscular junction in mutant larvae.

(Renger et al., 2000)

Mutants exhibit normal circadian pacemakers that can be entrained by light-dark cycles. Mutants display arrhythmic locomotor activity, rhythmic eclosion persists so eclosion occurs as in wild type.

(Majercak et al., 1997)

Pka-C1^X4 hemizygotes die as third-instar larvae at 18[o]C, the restrictive temperature, but are viable when raised at 25[o]C.

At 25[o]C, Pka-C1^X4 hemizygotes exhibit reduced initial learning relative to controls but normal memory decay in a Pavlovian olfactory learning assay. Shifting the temperature from 25[O]C to 18[o]C prior to training reduces initial learning to a similar extent to Pka-C1^X4 hemizygotes and controls but results in a steeper memory decay curve only in Pka-C1^X4 hemizygotes.

(Li et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Pka-C1^X4/Pka-C1^MB581 is partially rescued by Scer\GAL4^Mef2.247/Pka-C1^UAS.cKa

(Hong et al., 2008)

Pka-C1^X4/Pka-C1^MB581 is partially rescued by Scer\GAL4^c309/Pka-C1^UAS.cKa

(Hong et al., 2008)

Comments

Expression of Pka-C1^Scer\UAS.cKa under the control of either Scer\GAL4^Mef2.247 or Scer\GAL4^c309 almost completely rescues the temperature preference behavior defects seen in Pka-C1^MB581/Pka-C1^X4 mutant.

(Hong et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

DC0^X4

(Hong et al., 2008, Yao and Wu, 2001, Li et al., 1996, Li and Kalderon, 1993)

DCO^X4

(Hong et al., 2008, Renger et al., 2000, Majercak et al., 1997)

PKA^X4

(Li et al., 1996)

Pka-C1^X4

(Hong et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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