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General Information
Symbol
Dmel\Pka-C1X4
Species
D. melanogaster
Name
FlyBase ID
FBal0066002
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
DCOX4, DC0X4
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Pka-C1X4/Pka-C1Tw2 mutant flies show an inability to find a preferred temperature, with temperature preference spread across a wide gradient.

    Pka-C1X4/Df(2L)γ15 mutant flies show an inability to find a preferred temperature, with temperature preference spread across a wide gradient.

    Pka-C1X4/Pka-C1MB581 mutant flies show an inability to find a preferred temperature, with temperature preference spread across a wide gradient.

    The firing frequency is significantly increased in adaptive and tonic Pka-C1X4 neurons. The action potential amplitude is significantly decreased in adaptive but slightly reduced in tonic neurons in Pka-C1X4 mutant neurons. Pka-C1X4 mutant neurons display erratic firing rates during current injection.

    When Pka-C1X4 mutant neurons are simulated by step current injections, the firing pattern, in particular the number of spikes in each spike train, varied to a much greater extent than that in wild-type cells.

    As in wild-type, there is little variability in the time to peak of evoked ejcs in mutant boutons. There is a reduction in excitatory junctional current amplitude at the neuromuscular junction in mutant larvae.

    Mutants exhibit normal circadian pacemakers that can be entrained by light-dark cycles. Mutants display arrhythmic locomotor activity, rhythmic eclosion persists so eclosion occurs as in wild type.

    Pka-C1X4 hemizygotes die as third-instar larvae at 18[o]C, the restrictive temperature, but are viable when raised at 25[o]C.

    At 25[o]C, Pka-C1X4 hemizygotes exhibit reduced initial learning relative to controls but normal memory decay in a Pavlovian olfactory learning assay. Shifting the temperature from 25[O]C to 18[o]C prior to training reduces initial learning to a similar extent to Pka-C1X4 hemizygotes and controls but results in a steeper memory decay curve only in Pka-C1X4 hemizygotes.

    External Data
    Interactions
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    Phenotypic Class
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments

    Expression of Pka-C1Scer\UAS.cKa under the control of either Scer\GAL4Mef2.247 or Scer\GAL4c309 almost completely rescues the temperature preference behavior defects seen in Pka-C1MB581/Pka-C1X4 mutant.

    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (5)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (7)