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General Information
Symbol
Dmel\Nint.G.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0066007
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Nintra, UAS-Nintra, UAS-Nact, UAS-NICD, Nintra, UAS-NactW, UAS-Notchintra
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Carries an internal deletion in the N coding region that results in a weaker N activity than for Nint.SH.UAS.

Intracellular domain of N protein is expressed from UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of Nint.G.Scer\UAS under the control of Scer\GAL4sca-C253 leads to the virtual loss of thoracic sensory macrochaeta and microchaeta in adults, as compared to controls.

Under control conditions, the adulthood-only expression of Nint.G.UAS under the control of Scer\GAL4esg-NP7397 (and Gal80[ts], for the temporal regulation of expression) leads to a significant decrease in the number of eggs laid by females, as compared to controls.

Expression of Nint.G.Scer\UAS under the control of Scer\GAL4ptc.PU results in the production of cuticle folds along the proximo-distal axis of the leg that resemble ectopic joints.

Nint.G.Scer\UAS clones induced in type II neuroblasts (NBs) under the control of Scer\GAL4αTub84B.PL contain ectopic NB-like cells (Dpn and Mira positive).

There is a reduction in the number of SELK neurons in animals expressing Nint.G.Scer\UAS under the control of Scer\GAL4elav-C155.

Expression of Nint.G.Scer\UAS in glial cells, driven by Scer\GAL4repo, leads to an irregular migration of peripheral glial cells. The dorsal intersegmental peripheral glial cells do not migrate to their normal ventral position.

Adults carrying Nint.G.Scer\UAS, Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B and raised at the permissive temperature have wild-type midguts (at this temperature, the Scer\GAL80ts.αTub84B protein inhibits Scer\GAL4esg-NP5130). When these flies are shifted to the non-permissive temperature (which inactivates Scer\GAL80ts.αTub84B, allowing expression of Nint.G.Scer\UAS under the control of Scer\GAL4esg-NP5130), some esg+ cells in the midgut show an increase in nuclear size.

Expression of Nint.G.Scer\UAS under the control of Scer\GAL4GMR.PF produces excess mitoses in the eye disc.

Embryos expressing Nint.G.Scer\UAS under the control of Scer\GAL4Kr.PM show an almost complete loss of neurons in parasegments 4-6.

Expression of Nint.G.Scer\UAS under the control of Scer\GAL4sca-C253 removes essentially all bristles on the notum.

When Nint.G.Scer\UAS is driven by Scer\GAL4eve.eme no DA1 muscles are formed and in 60% of hemisegments an increase in eve expressing pericardial cells (EPCs) is seen from two to three or four. An associated loss of DA1 and DO2 muscle precursors is seen.

Nint.G.Scer\UAS driven by Scer\GAL4ap-md544 causes overproliferation in the wing disc.

In somatic clones where the expression of Nint.G.Scer\UAS is under the control of Scer\GAL4Ubx.PdC various phenotypes are seen. Clones in the wing pouch of both surfaces cause wing outgrowths. The outgrowths contain small territories of mutant cells in their tip that differentiate wing margin pattern elements and wild-type cells in their base that differentiate wing blade tissue with identifiable vein patterns. The largest outgrowths appear in clones close to the anterior posterior boundary, away from the dorsal ventral boundary. Somatic clones where the expression of Nint.G.Scer\UAS is under the control of Scer\GAL4Act5C.PI grow normally, without causing outgrowths.

When expression is driven by Scer\GAL4btl.PS, dorsal and lateral truck fusion is prevented. Some dorsal branches extend an extra terminal branch. In 20% of dorsal branches cell markers suggest the presumptive fusion cell is converted to a terminal cell.

dMP2 neurons project their axons in an anterior direction, along the pathway of the vMP2, in embryos expressing Nint.G.Scer\UAS under the control of Scer\GAL4ftz.ng.

Animals expressing Nint.G.Scer\UAS under the control of Scer\GAL41151 lack both dorsal longitudinal and dorsoventral muscles. The direct flight muscles (DFMs) appear relatively normal, although DFM 54 is thinner than normal.

When expression is driven by Scer\GAL4ey.PH, the eye is enlarged, mainly in the dorso-ventral axis.

Embryos expressing Nint.G.Scer\UAS under the control of Scer\GAL4twi.PG and Scer\GAL4how-24B show no heart formation. The specification and/or differentiation of the visceral mesoderm and somatic muscles is also abolished.

Visceral mesoderm does not form in embryos expressing Nint.G.Scer\UAS under the control of both Scer\GAL4how-24B and Scer\GAL4twi.PG.

Expression of Nint.G.Scer\UAS under the control of Scer\GAL4dpp.blk1 results in ectopic joint structures in the leg. The strength of the phenotype depends on the P{UAS-N.intra.G} line used. Weakly expressing lines develop normal size legs which develop partial ectopic joint structures, particularly in the tibia and first tarsal segment. The ectopic joint structures are restricted to the dorsal side of the leg. Flies that express Nint.G.Scer\UAS more strongly have legs with extremely abnormal morphology; the tibia and femur are bifurcated by an abnormal proximal-distal fold, and connections between tarsal segments are also affected, with many joint structures appearing in abnormal positions.

Embryos expressing Nint.G.Scer\UAS under the control of Scer\GAL4C612b show an increase in the number of adult muscle precursors at the expense of the dorsal muscles.

When expression is driven by Scer\GAL4Ubx.PdC in clones in the wing, outpocketings of the wing occur which include both Nint.G.Scer\UAS-expressing and neighboring wild type cells. Ectopic wing margin bristles appear in both mutant and wild type cells. The cells are smaller and have altered polarity.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
NOT suppressed by
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The loss of thoracic sensory macrochaeta and microchaeta that results from the expression of Nint.G.Scer\UAS under the control of Scer\GAL4sca-C253 is not suppressed by co-expressing Rbfox1dsRNA.Scer\UAS.

dysf2/dysf3 does not suppress the production of cuticle folds along the proximo-distal axis of the leg that resemble ectopic joints seen when Nint.G.Scer\UAS is expressed under the control of Scer\GAL4ptc.PU (and limited to the third instar larval stage using Scer\GAL80ts.αTub84B). As in dysf2/dysf3 mutants alone, endogenous tarsal joints are not formed.

Su(H)del47 suppresses the presence of ectopic NB-like cells (Dpn and Mira positive) phenotype seen when Nint.G.Scer\UAS clones are induced in third instar larval type II NBs under the control of Scer\GAL4αTub84B.PL.

dpn7 does not suppress the ectopic neuroblast phenotype seen when clones expressing Nint.G.Scer\UAS are induced under the control of Scer\GAL4αTub84B.PL.

Co-expression of Nint.G.Scer\UAS with kuzDN.Scer\UAS in egg chamber border cells (under the control of Scer\GAL4slbo.2.6) ameliorates the migration defect observed in kuzDN.Scer\UAS mutants.

The loss of neurons seen in embryos expressing Nint.G.Scer\UAS under the control of Scer\GAL4Kr.PM is suppressed by ed2B8/edts at 29oC.

The lack of bristles phenotype on the notum seen in flies expressing Nint.G.Scer\UAS under the control of Scer\GAL4sca-C253 is not modified by co-expression of edΔECD.Scer\UAS.

The addition of numbfl.Scer\UAS.T:Hsap\MYC to Nint.G.Scer\UAS, Scer\GAL4eve.eme embryos attenuates the Nint.G.Scer\UAS phenotype - more DA1 muscles form and less EPCs form.

Strongly suppresses the wing nicking phenotype seen in mamN.Scer\UAS, Scer\GAL4C96 flies. mamN.Scer\UAS does not suppress the bristle phenotype seen in Nint.G.Scer\UAS, Scer\GAL4C96 flies.

Does not suppress the mam3.177 vMP2 neuron phenotype when expressed under the control of Scer\GAL4ftz.ng.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

M. Go and S. Artavanis-Tsakonas.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (46)