FlyBase Allele Report: Dmel\Dad[UAS.cTa]

The adulthood-only expression of Dad^Scer\UAS.cTa under the control of Scer\GAL4^esg.PU, Scer\GAL4^dsx.KI or Scer\GAL4^spi-NP0261 (in combination with Gal80[ts] for the temporal regulation of expression) leads to a significant decrease in the relative and absolute size of secondary cell nuclei compared to neighboring main cells and wild-type controls; adulthood-only expression under the combined control of Scer\GAL4^esg.PU and Gal80[ts] does not affect the size of main cell nuclei compared to controls. The adulthood-only expression under the control of Scer\GAL4^dsx.KI (and Gal80[ts]) leads to a significant decrease in the number of secretory vesicles within accessory gland secondary cells, associated with a significant decrease in the size of the largest multivesicular body/lysosome, as compared to controls; there is also a severe decrease in the secretion of (CD63-positive) exosomes into the accessory gland lumen. The adulthood-only expression under the control of Scer\GAL4^spi-NP0261 (and Gal80[ts]) leads to a significant increase in the size of the largest multivesicular body/lysosome within accessory gland secondary cells, as compared to controls.

The adulthood-only expression of Dad^Scer\UAS.cTa under the combined control of Scer\GAL4^spi-NP0261 and Gal80[ts] also results in both virgin and mated males showing a significant increase in the number of dense core granule compartments within accessory gland secondary cells, as compared to matched controls.

(Redhai et al., 2016)

Expression of Dad^Scer\UAS.cTa in secondary cells under the control of Scer\GAL4^dsx.KI reduces exosome secretion into the accessory gland lumen compared to controls. Intraluminal vesicles inside MVBs or lysosomes (MVBLs) in male secondary cells are increased compared to controls when Dad^Scer\UAS.cTa is expressed under the control of Scer\GAL4^spi-NP0261.

(Corrigan et al., 2014)

Expression of Dad^Scer\UAS.cTa under the control of Scer\GAL4^elav-C155 results in vacuolisation in the brains of 20 day old flies.

(Nahm et al., 2013)

Expression of Dad^Scer\UAS.cTa under the control of Scer\GAL4^vg.PM results in a dramatic reduction in size of the wing and wing patterning defects.

(Xu et al., 2013)

Expression of Dad^Scer\UAS.cTa with Scer\GAL4^71B suppresses formation of the anterior crossvein and interrupts veins L4 and L5.

(Liu et al., 2011)

Somatic clones of Dad^Scer\UAS.cTa expressing fat body cells driven by Scer\GAL4^Act5C.PI show a cell autonomous increase in fluorescently labeled fatty acid uptake compared to wild-type cells.

(Ballard et al., 2010)

Embryos expressing Dad^Scer\UAS.cTa in the Malpighian tubules under the control of Scer\GAL4^byn-Gal4 do not display normal anterior tubule migration, and the anterior pair of tubules project posteriorly in these animals.

(Bunt et al., 2010)

Histoblasts expressing Dad^Scer\UAS.cTa under the control of Scer\GAL4^{Scer\FRT.Act5C} (initiated by heat-shock) prior to the onset of epithelial expansion join part of the periphery of the nest and display normal cell shape and size. However, upon initiation of histoblast spreading, the Dad^Scer\UAS.cTa-expressing histoblasts become highly condensed and are rapidly excluded from the periphery.

(Ninov et al., 2010)

Scer\GAL4¹¹⁵¹ driven Dad^Scer\UAS.cTa engenders a severe reduction in the number of tergal depressor of trochanter (TDT) muscle fibers.

There are significantly fewer TDT founder cells compared to control flies observed 16 hours after puparium formation (APF) as a result of Scer\GAL4¹¹⁵¹ driven Dad^Scer\UAS.cTa expression.

(Jaramillo et al., 2009)

Posterior overexpression of Dad^Scer\UAS.cTa by Scer\GAL4^en-e16E leads to ectopic apical fold formation along the anteroposterior boundary of the larval wing pouch.

(Shen et al., 2008)

Expression of Dad^Scer\UAS.cTa under the control of Scer\GAL4^CY2 results in the eggshells with a smaller operculum and deformed dorsal appendages.

(Yakoby et al., 2008)

Expression of Dad^Scer\UAS.cTa under the control of Scer\GAL4^CY2 results in the dorsal appendages being shifted towards the anterior edge of the egg chamber.

(Zartman et al., 2008)

Expression of Dad^Scer\UAS.cTa using Scer\GAL4^salm-459.2 results in moderate wing size reduction and vein loss.

(Barrio et al., 2007)

Expression of Dad^Scer\UAS.cTa in the peripheral amnioserosa (under the control of Scer\GAL4^pAS) results in the progression of the peripheral amnioserosa being delayed. The peripheral amnioserosa becomes highly disorganised and its purse string F-actin is reduced and shows rapidly moving filipodia along the entire outer side.

(Wada et al., 2007)

Expression of Dad^Scer\UAS.cTa under the control of Scer\GAL4^dpp.3KK results in loss of wing veins.

(Molnar et al., 2006)

Expression of Dad^Scer\UAS.cTa under the control of Scer\GAL4^dpp.s-hc results in 58% of flies showing head capsule defects. These include vibrissae defects and reduced maxillary palps.

(Stultz et al., 2006)

Expression of Dad^Scer\UAS.cTa under the control of Scer\GAL4^nos.PG results in a reduction of the number of germline stem cells compared to wild-type germaria.

(Ward et al., 2006)

Wing discs that express Dad^Scer\UAS.cTa under the control of Scer\GAL4^{Ubx-lac1-Gal4} display aberrant squamous morphogenesis. In some discs, the peripodial epithelium consists entirely of cuboidal-shaped cells, while in others there is a peripodial epithelium with limited squamous cells apposed to clefted folds in the disc proper. The anterioposterior compartment boundary in the peripodial epithelium fails to shift in the absence of squamous morphogenesis. Additionally, these wing discs are slighly smaller than control discs.

(McClure and Schubiger, 2005)

Somatic clones of Dad^Scer\UAS.cTa; Scer\GAL4^{Act5C.Switch.PR} cells in mid-third instar wing discs show autonomous decreases in numbers of cells in S-phase after 14 hours treatment with 20μg/ml RU486. However, if the edges of these clones are located medially they cause a large increase in the of cells in S-phase adjacent to the medially located edge of the clone. These effects are weaker than those seen with brk^Scer\UAS.cJa.

(Rogulja and Irvine, 2005)

Overexpression of Dad^Scer\UAS.cTa, under the control of Scer\GAL4^bi-omb-Gal4 generates defects in the pattern of R axon projection with a penetrance of approximately 40%. Differentiation of lamina neurons is also impaired, along with defects in the formation of the regular crescent shape of the lamina. Epithelial, marginal and medulla glia layers are not clearly distinguishable, with cells irregularly spaced and exhibiting a defect in cell shape. Glial cells fail to develop their characteristic cuboidal shape and look irregular.

(Yoshida et al., 2005)

Expression of Dad^Scer\UAS.cTa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} induces differentiation of primordial germ cells in larval ovaries, with 16-cell cysts already being observed at the end of the second larval instar.

(Gilboa and Lehmann, 2004)

In Dad^Scer\UAS.cTa; Scer\GAL4^btl.PS embryos, terminal tracheal branches are frequently misdirected. Some extend along the anterior-posterior axis. If such branches reach the adjacent segment, they often bend along the dorsal-ventral axis. Other misdirected terminal branches extend dorsally instead of ventrally, and cross the dorsal midline.

(Kato et al., 2004)

The testes of Dad^Scer\UAS.cTa; Scer\GAL4^{nos.UTR.T:Hsim\VP16} adult males contain no germline stem cells.

(Kawase et al., 2004)

The wing pouch in late third instar wing discs from Dad^Scer\UAS.cTa; Scer\GAL4^nub-AC-62 is dramatically reduced in size. Wings in the resulting adults have are reduced to tiny residual stubs.

(Martin et al., 2004)

Expression of Dad^Scer\UAS.cTa under the control of Scer\GAL4^OK6 results in a dramatic reduction in neuromuscular junction size.

(McCabe et al., 2004)

Expression of Dad^Scer\UAS.cTa under the control of Scer\GAL4^btl.PS results in loss of dorsal branch formation in the tracheal system.

(Ribeiro et al., 2004)

Expression of Dad^Scer\UAS.cTa under the control of Scer\GAL4^fkh.PH does not cause defects in salivary gland migration.

(Bradley et al., 2003)

Testes expressing Dad^Scer\UAS.cTa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} show a range of phenotypes at 25^oC. In 27% of cases, the testes appear wild type. In 21% of cases, the testes appear thinner and smaller than normal and have a visible reduction in the number of germ cells. In the remaining 52% of cases, the testes have degenerated and completely lack germ line stem cells, spermatogonia and spermatocytes.

(Shivdasani and Ingham, 2003)

When Dad^Scer\UAS.cTa clones are made in the developing wing disc under the expression of Scer\GAL4^Act5C.PI cell death phenotypes can be seen. 24 hours after clone induction, apoptosis is seen to begin around the wing tip primordium. 36 hours after induction, removal of clones from the wing disc primordium is almost complete. 48 hours after induction, clones only survive outside wing blade primordium.

(Adachi-Yamada and O'Connor, 2002)

When expression is driven by Scer\GAL4^Gug-AGiR wing discs are small and abnormal, with clefts across the blade primordium. Peripodial membrane remains intact - discs can evaginate normally and give rise to adult cuticle. Adult wings can be small with duplicated margins or occasionally split in half along the AP boundary. At 29^oC 15/15 wings were narrower and shorter than wild type conbtrols. When expression si driven by Scer\GAL4^c311 eye discs are reduced and devoid of squamous peripodial cells - lacking pattern or neuronal differentiation.

(Gibson et al., 2002)

When Dad^Scer\UAS.cTa is driven by Scer\GAL4^A9, a reduction in disc size. This reduction is especially pronounced along the anterior posterior axis.

(Martin-Castellanos and Edgar, 2002)

The tracheal systems of Dad^Scer\UAS.cTa; Scer\GAL4^btl.PS embryos and larvae lack dorsal branches, although occasional stump-like dorsal outgrowths form in their place. In rare cases these stumps form a dorsal trunk sized lumen. Observation of live embryos shows that dorsal outgrowths and filopodial activities do occur at positions where dorsal branches normally form. These outgrowths look 'bud-like' and form filopodial extensions, but never refine to single cell diameter dorsal branches, and usually reintegrate into the main dorsal trunk.

(Ribeiro et al., 2002)

When expression is driven by Scer\GAL4^Bx-MS1096 the wings are tiny and veinless.

(Marquez et al., 2001)

Expression of Dad^Scer\UAS.cTa under the control of Scer\GAL4^Dll-md23 results in fewer wing and leg disc cells in embryos.

(Kubota et al., 2000)

When expression is driven by Scer\GAL4^vg.PM the wing loses its margin and in extreme cases only a tiny winglet forms.

(Tsuneizumi et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dad^UAS.cTa, Scer\GAL4^vg.PM has visible phenotype, suppressible | partially by Art1^UAS.EGFP, Scer\GAL4^vg.PM

(Xu et al., 2013)

BacA\p35^UAS.cHa, Dad^UAS.cTa, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B has abnormal cell shape | conditional | third instar larval stage phenotype, suppressible by Rho1^N19.UAS, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B

(Widmann and Dahmann, 2009)

BacA\p35^UAS.cHa, Dad^UAS.cTa, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B has abnormal cell shape | conditional | third instar larval stage phenotype, suppressible by Mbs^N300.UAS, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B

(Widmann and Dahmann, 2009)

BacA\p35^UAS.cHa, Dad^UAS.cTa, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B has abnormal cell shape | conditional | third instar larval stage phenotype, suppressible by sqh^GD1695, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B

(Widmann and Dahmann, 2009)

Dad^UAS.cTa, Scer\GAL4^Act5C.PP has FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone phenotype, suppressible | partially by BacA\p35^UAS.cHa, Scer\GAL4^Act5C.PP

(Martin-Castellanos and Edgar, 2002)

Suppressor of

Statement

Reference

Scer\GAL4^esg.PU, Dad^UAS.cTa is a suppressor | partially of abnormal cell cycle | male | adult stage phenotype of Cdk4^UAS.cMa, CycD^UAS.cUa, Scer\GAL4^esg.PU

(Sekar et al., 2023)

Scer\GAL4^esg.PU, Dad^UAS.cTa is a suppressor of increased cell growth rate | male | adult stage phenotype of Cdk4^UAS.cMa, CycD^UAS.cUa, Scer\GAL4^esg.PU

(Sekar et al., 2023)

Scer\GAL4^nub-AC-62/Dad^UAS.cTa is a suppressor of neoplasia | somatic clone | third instar larval stage phenotype of brk^M68

(Martin et al., 2004)

Scer\GAL4^nub-AC-62/Dad^UAS.cTa is a suppressor of increased occurrence of cell division | somatic clone | third instar larval stage phenotype of brk^M68

(Martin et al., 2004)

Dad^UAS.cTa, Scer\GAL4^vg.PM is a suppressor of visible phenotype of Scer\GAL4^vg.PM, tkv^{Q253D.UAS.cNb}

(Tsuneizumi et al., 1997)

Dad^UAS.cTa, Scer\GAL4^vg.PM is a suppressor of visible phenotype of Mad^UAS.cNa, Scer\GAL4^vg.PM

(Tsuneizumi et al., 1997)

NOT Suppressor of

Statement

Reference

Scer\GAL4^esg.PU, Dad^UAS.cTa is a non-suppressor of abnormal cell cycle | male | adult stage phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^esg.PU

(Sekar et al., 2023)

Scer\GAL4^esg.PU, Dad^UAS.cTa is a non-suppressor of increased cell growth rate | male | adult stage phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^esg.PU

(Sekar et al., 2023)

Scer\GAL4^nub-AC-62/Dad^UAS.cTa is a non-suppressor of increased cell number | somatic clone phenotype of brk^M68

(Martin et al., 2004)

Other

Statement

Reference

Phenotype Manifest In

Suppressed by

Statement

Reference

Dad^UAS.cTa, Scer\GAL4^vg.PM has wing phenotype, suppressible | partially by Art1^UAS.EGFP, Scer\GAL4^vg.PM

(Xu et al., 2013)

Dad^UAS.cTa, Scer\GAL4^71B has wing vein phenotype, suppressible by Lpin^GD14004, Scer\GAL4^71B

(Liu et al., 2011)

BacA\p35^UAS.cHa, Dad^UAS.cTa, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B has wing disc | conditional | third instar larval stage phenotype, suppressible by Rho1^N19.UAS, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B

(Widmann and Dahmann, 2009)

BacA\p35^UAS.cHa, Dad^UAS.cTa, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B has wing disc | conditional | third instar larval stage phenotype, suppressible by Mbs^N300.UAS, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B

(Widmann and Dahmann, 2009)

BacA\p35^UAS.cHa, Dad^UAS.cTa, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B has wing disc | conditional | third instar larval stage phenotype, suppressible by sqh^GD1695, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B

(Widmann and Dahmann, 2009)

Dad^UAS.cTa, Scer\GAL4^btl.PS has dorsal tracheal branch primordium phenotype, suppressible by arm²

(Ribeiro et al., 2004)

Suppressor of

Statement

Reference

Scer\GAL4^esg.PU, Dad^UAS.cTa is a suppressor | partially of male accessory gland secondary cell phenotype of Cdk4^UAS.cMa, CycD^UAS.cUa, Scer\GAL4^esg.PU

(Sekar et al., 2023)

Scer\GAL4^Act.PU/Dad^UAS.cTa is a suppressor of wing vein phenotype of Ctdnep1^G0269

(Liu et al., 2011)

Dad^UAS.cTa, Scer\GAL4^nanos.PG is a suppressor | partially of female germline stem cell | increased number phenotype of Delta^UASp.cJa, Scer\GAL4^nanos.PG

(Ward et al., 2006)

Dad^UAS.cTa, Scer\GAL4^vg.PM is a suppressor of wing phenotype of Scer\GAL4^vg.PM, tkv^{Q253D.UAS.cNb}

(Tsuneizumi et al., 1997)

Dad^UAS.cTa, Scer\GAL4^vg.PM is a suppressor of wing phenotype of Mad^UAS.cNa, Scer\GAL4^vg.PM

(Tsuneizumi et al., 1997)

NOT Suppressor of

Statement

Reference

Scer\GAL4^esg.PU, Dad^UAS.cTa is a non-suppressor of male accessory gland secondary cell phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^esg.PU

(Sekar et al., 2023)

Other

Statement

Reference

BacA\p35^UAS.cHa, Dad^UAS.cTa, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B has wing disc | conditional | third instar larval stage phenotype

(Widmann and Dahmann, 2009)

BacA\p35^UAS.cHa, Dad^UAS.cTa, Scer\GAL4^nub-AC-62, Scer\GAL80^ts.αTub84B has wing disc | conditional | third instar larval stage phenotype

(Widmann and Dahmann, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Art1^{Scer\UAS.T:Avic\GFP-EGFP} rescues the wing size and morphology defects caused by expression of Dad^Scer\UAS.cTa under the control of Scer\GAL4^vg.PM, with the degree of rescue being dose-dependent.

(Xu et al., 2013)

Scer\GAL4^Act.PU-mediated expression of Dad^Scer\UAS.cTa completely rescues the vein phenotype of Dd^G0269/Y.

Co-expression of Lpin^GD14004 efficiency represses the wing vein phenotype of Scer\GAL4^71B, Dad^Scer\UAS.cTa flies.

(Liu et al., 2011)

Coexpression of Dad^Scer\UAS.cTa with Dl^{Scer\UAS.P\T.cJa}, under the control of Scer\GAL4^nos.PG, partially suppresses the additional germ-line stem cell of females that express only Dl^{Scer\UAS.P\T.cJa}.

(Ward et al., 2006)

Embryos expressing Dad^Scer\UAS.cTa under the control of Scer\GAL4^btl.PS in an arm² background show outgrowth of the dorsal branches, cell intercalation and autocellular adherens junction formation in the tracheal system.

(Ribeiro et al., 2004)

The addition of BacA\p35^Scer\UAS.cHa means that Dad^Scer\UAS.cTa clones (driven by Scer\GAL4^Act5C.PP) do survive though these clones still proliferate more slowly than wild-type.

(Martin-Castellanos and Edgar, 2002)

Suppresses the wing outgrowth phenotype of tkv^{Q253D.Scer\UAS.cNb} or Mad^Scer\UAS.cNa driven by Scer\GAL4^vg.PM, resulting in an almost wild type wing.

(Tsuneizumi et al., 1997)

Xenogenetic Interactions

Statement

Reference

48 hours after inducing expression of Dad^Scer\UAS.cTa and BacA\p35^Scer\UAS.cHa in the dorsal compartment of the wing disc (under the temperature-regulated control of Scer\GAL4^ap-md544 by Scer\GAL80^ts.αTub84B), mutant cells are significantly shorter along their apical-basal axis compared to controls. After 56 hours, mutant cells are further shortened, and a deep apical invagination and basal indentation are observed at the border with the dorsal/ventral compartment boundary. Cell volume is conserved in mutant cells. This phenotype is rescued by co-expression of Rho1^N19.Scer\UAS, sqh^GD1695 or Mbs^{N300.Scer\UAS}.

Overexpression of Dad^Scer\UAS.cTa and BacA\p35^Scer\UAS.cHa in the whole wing disc (under the temperature-regulated control of Scer\GAL4^nub-AC-62 by Scer\GAL80^ts.αTub84B), results in shorter and apically wider cells.

(Widmann and Dahmann, 2009)

bi reduction caused by the posterior expression of Dad^Scer\UAS.cTa elicited a strong anteroposterior fold which was unaffected by coexpression of BacA\p35^Scer\UAS.cHa

(Shen et al., 2008)

The addition of BacA\p35^Scer\UAS.cHa means that Dad^Scer\UAS.cTa clones (driven by Scer\GAL4^Act5C.PP) do survive though these clones still proliferate more slowly than wild-type.

(Martin-Castellanos and Edgar, 2002)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

98452

y¹ w^*; P{UAS-Dad.T}T

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dad^Scer\UAS.cTa

(Corrigan et al., 2014)

Dad^UAS.cTa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Tsuneizumi

(Tsuneizumi et al., 1997)

Secondary FlyBase IDs

References (68)

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