Allele Dmel\tgo^DN.Scer\UAS

General Information
Symbol	Dmel\tgo^DN.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0082183
Feature type	allele	Associated gene	Dmel\tgo

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - site directed mutagenesis, in vitro construct - amino acid replacement
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Ohshiro and Saigo, 1997) in vitro construct - regulatory fusion (Ohshiro and Saigo, 1997) in vitro construct - site directed mutagenesis (Ohshiro and Saigo, 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: A dominant negative form of tgo, in which a 13 amino acid basic region (SRENHCEIERRRR) is replaced by the sequence GIL is expressed under the control of four copies of the Scer\UAS regulatory sequence and a heat shock promoter. (Ohshiro and Saigo, 1997)
Carried in construct	P{UAS-tgo.DN} (Ohshiro and Saigo, 1997, Umetsu et al., 2006, Schmidt et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	cell migration defective, with Scer\GAL4^loco.1.3 (Schmidt et al., 2011) neuroanatomy defective, with Scer\GAL4^loco.1.3 (Schmidt et al., 2011) neuroanatomy defective, with Scer\GAL4^NP6099 (Umetsu et al., 2006)
Phenotype Manifest In
	embryonic/larval brain, with Scer\GAL4^NP6099 (Umetsu et al., 2006) embryonic/larval tracheal system, with Scer\GAL4^btl.PS (Ohshiro and Saigo, 1997) exit glial cell, with Scer\GAL4^loco.1.3 (Schmidt et al., 2011) glial cell, with Scer\GAL4^loco.1.3 (Schmidt et al., 2011) lamina forming neuroblast, with Scer\GAL4^NP6099 (Umetsu et al., 2006)
Detailed Description
	Statement Reference Expression of tgo[DN.Scer\UAS] in all glial cells, using Scer\GAL4[repo] does not result in any impairment of glial migration. Expression of tgo[DN.Scer\UAS] in all tracheal cells using Scer\GAL4[btl.PS] does not lead to a glial migration phenotype. Expression of tgo[DN.Scer\UAS] under the control of Scer\GAL4[loco.1.3] causes a phenocopy of the glial migration phenotype of tgo mutant embryos in the absence of a CNS defect. (Schmidt et al., 2011) In brains of larvae that express tgo^DN.Scer\UAS in developing lamina neurons, driven by Scer\GAL4^NP6099, all of the R axon bundles are packed tightly in the posterior domain of the lamina region and almost all of the developing lamina neurons are located outside of the R axon bundles. (Umetsu et al., 2006) Causes stalling of tracheal migration when expressed using Scer\GAL4^btl.PS. (Ohshiro and Saigo, 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^NP6099, tgo^DN.Scer\UAS has neuroanatomy defective phenotype, suppressible by Scer\GAL4^NP6099/sim^Scer\UAS.cCa (Umetsu et al., 2006)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^NP6099, tgo^DN.Scer\UAS has embryonic/larval brain phenotype, suppressible by Scer\GAL4^NP6099/sim^Scer\UAS.cCa (Umetsu et al., 2006) Scer\GAL4^NP6099, tgo^DN.Scer\UAS has lamina forming neuroblast phenotype, suppressible by Scer\GAL4^NP6099/sim^Scer\UAS.cCa (Umetsu et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference In larval brains that coexpress tgo^DN.Scer\UAS and sim^Scer\UAS.cCa, both under the control of Scer\GAL4^NP6099, both the arrangement of lamina neurons and the spacing of R axon bundles are almost indistinguishable from those of control brains. (Umetsu et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	tgo^DN.Scer\UAS tgo^DN.UAS
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Schmidt et al., 2011, Glia 59(9): 1264--1272 Transcriptional regulation of peripheral glial cell differentiation in the embryonic nervous system of drosophila. [FBrf0214270] Umetsu et al., 2006, Development 133(5): 791--800 The highly ordered assembly of retinal axons and their synaptic partners is regulated by Hedgehog/Single-minded in the Drosophila visual system. [FBrf0190327] Ohshiro and Saigo, 1997, Development 124(20): 3975--3986 Transcriptional regulation of breathless FGF receptor gene by binding of TRACHEALESS/dARNT heterodimers to three central midline elements in Drosophila developing trachea. [FBrf0099429]

Allele Dmel\tgoDN.Scer\UAS

Allele Dmel\tgo^DN.Scer\UAS