Allele Dmel\pnt^unspecified

General Information
Symbol	Dmel\pnt^unspecified	Species	D. melanogaster
Name		FlyBase ID	FBal0082324
Feature type	allele	Associated gene	Dmel\pnt

Allele class
Mutagen
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| recessive (Tearle and Nusslein-Volhard, 1987) lethal \| recessive (Therrien et al., 2000)
Phenotype Manifest In
	anterior commissure (Stollewerk and Klaembt, 1997) cardioblast \| ectopic \| somatic clone (Alvarez et al., 2003) cephalopharyngeal skeleton (Tearle and Nusslein-Volhard, 1987) commissure (Hummel et al., 1999, Hummel et al., 1999, Stollewerk and Klaembt, 1997) denticle belt (Tearle and Nusslein-Volhard, 1987) embryonic/larval dorsal branch & terminal tracheal cell \| somatic clone (Ghabrial and Krasnow, 2006) longitudinal connective (Hummel et al., 1999) midline glial cell (Hummel et al., 1999, Hummel et al., 1999) motor neuron (Hummel et al., 1999) presumptive embryonic/larval central nervous system (Hummel et al., 1999, Hummel et al., 1999, Tearle and Nusslein-Volhard, 1987) presumptive embryonic/larval peripheral nervous system (Hummel et al., 1999) sensory neuron (Hummel et al., 1999)
Detailed Description
	Statement Reference Mosaic dorsal tracheal branches lack a terminal cell in 26% of cases. In 80% of the cases where the terminal cell is missing, a homozygous mutant cell is located in the stalk position nearest the tip. (Ghabrial and Krasnow, 2006) When homozygous pnt cardioblast clones are made in the embryo, a small proportion of clones exhibit more cardioblasts than expected, suggesting a slight increase in the proliferative capability of cardioblast precursors. (Alvarez et al., 2003) Fas2-positive nerve bundles are seen to cross the midline in mutant embryos, in contrast to wild-type. (Hummel et al., 1999) Mutant embryos show a clear fusion of commissures and frequent disruption of longitudinal connectives. Midline glial cells are either reduce in number or fail to properly migrate in between anterior and posterior commissures. General embryonic morphology is relatively normal, though the PNS shows a slight reduction in sensory neurons and defects in the trajectories of motoneurons. (Hummel et al., 1999) The axon bundles of the anterior commissures are not organised into three sub-regions in homozygous embryos, in contrast to wild-type. One or two discrete axon bundles can be seen within the commissure. The commissural axons leave the longitudinal tracts at apparently random, irregular positions along the dorso-ventral axis of the connective. (Stollewerk and Klaembt, 1997) pnt^unspecified mutant embryos have narrow denticle bands. The central nervous system is broad and less dense than wild-type. The head skeleton has fused ventral arms. (Tearle and Nusslein-Volhard, 1987)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference pnt^unspecified/pnt[+] is an enhancer of lethal phenotype of phl¹² (Therrien et al., 2000) pnt^unspecified/pnt[+] is an enhancer of visible phenotype of ksr::tor^{ΔNksr.hs.sev} (Therrien et al., 2000)
Suppressor of
Statement Reference pnt^unspecified/pnt[+] is a suppressor of visible phenotype of Ras85D^V12.sev (Therrien et al., 2000)
Phenotype Manifest In
Enhanced by
Statement Reference pnt^unspecified has commissure phenotype, enhanceable by foi^unspecified (Hummel et al., 1999) pnt^unspecified has commissure phenotype, enhanceable by sur^unspecified (Hummel et al., 1999)
Enhancer of
Statement Reference pnt^unspecified/pnt[+] is an enhancer of central nervous system phenotype of spen^AH393 (Chen and Rebay, 2000) pnt^unspecified/pnt[+] is an enhancer of eye phenotype of ksr::tor^{ΔNksr.hs.sev} (Therrien et al., 2000) pnt^unspecified/pnt[+] is an enhancer of longitudinal connective phenotype of spen^AH393 (Chen and Rebay, 2000) pnt^unspecified is an enhancer of commissure phenotype of foi^unspecified (Hummel et al., 1999) pnt^unspecified is an enhancer of commissure phenotype of sur^unspecified (Hummel et al., 1999)
Suppressor of
Statement Reference pnt^unspecified/pnt[+] is a suppressor of eye phenotype of Ras85D^V12.sev (Therrien et al., 2000)
Additional Comments
Genetic Interactions
Statement Reference foi^unspecified pnt^unspecified or sur^unspecified pnt^unspecified double mutant embryos show a slightly enhanced fused commissure phenotype compared to the phenotypes of the single mutants. The number of midline glial cells is reduced in the double mutant embryos. Commissures fail to develop in most neuromeres of Hem^unspecified pnt^unspecified double mutant embryos. (Hummel et al., 1999)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	pnt^unspecified
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Ghabrial and Krasnow, 2006, Nature 441(7094): 746--749 Social interactions among epithelial cells during tracheal branching morphogenesis. [FBrf0192962] Alvarez et al., 2003, Development 130(13): 3015--3026 pannier and pointedP2 act sequentially to regulate Drosophila heart development. [FBrf0160371] Chen and Rebay, 2000, Curr. Biol. 10(15): 943--946 split ends, a new component of the Drosophila EGF receptor pathway, regulates development of midline glial cells. [FBrf0129761] Therrien et al., 2000, Genetics 156(3): 1231--1242 A genetic screen for modifiers of a kinase suppressor of ras-dependent rough eye phenotype in Drosophila. [FBrf0131405] Hummel et al., 1999, Development 126(4): 771--779 Commissure formation in the embryonic CNS of Drosophila. [FBrf0106675] Hummel et al., 1999, Dev. Biol. 209(2): 381--398 Commissure formation in the embryonic CNS of Drosophila. [FBrf0108204] Stollewerk and Klaembt, 1997, Dev. Genes Evol. 207(6): 402--409 The midline glial cells are required for regionalization of commissural axons in the embryonic CNS of Drosophila. [FBrf0099896]
Stock list	Tearle and Nusslein-Volhard, 1987, D. I. S. 66: 209--269 Tubingen mutants and stock list. [FBrf0045941]

Allele Dmel\pntunspecified

Allele Dmel\pnt^unspecified