Allele Dmel\pnr^Scer\UAS.cHa

General Information
Symbol	Dmel\pnr^Scer\UAS.cHa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Haenlin	FlyBase ID	FBal0082339
Feature type	allele	Associated gene	Dmel\pnr

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Haenlin et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Wild-type pnr cDNA is expressed under the control of Scer\UAS regulatory sequences. (Haenlin et al., 1997)
Carried in construct	P{UAS-pnr.H} (Han and Olson, 2005, Herranz and Morata, 2001, Reim and Frasch, 2005, Klinedinst and Bodmer, 2003, Reim et al., 2005, Pena-Rangel et al., 2002, Ghazi et al., 2003, Calleja et al., 2000, Ramain et al., 2000, Gajewski et al., 1999, Sato and Saigo, 2000, Garcia-Garcia et al., 1999, Haenlin et al., 1997, Qian et al., 2005, Miskolczi-McCallum et al., 2005, Okumura et al., 2005, Mandal et al., 2004, Gajewski et al., 2001, Aldaz et al., 2003, Sellin et al., 2006, Biryukova and Heitzler, 2005, Akasaka et al., 2006, Akasaka et al., 2006, Qian and Bodmer, 2009, Pereira et al., 2006, de Navascués and Modolell, 2010, Ryu et al., 2011, Zenvirt et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	planar polarity defective \| cell non-autonomous \| somatic clone, with Scer\GAL4^Ubx.PdC (Calleja et al., 2000) viable, with Scer\GAL4^pnr-MD237 (Herranz and Morata, 2001) visible, with Scer\GAL4^C-765 (Garcia-Garcia et al., 1999, de Navascués and Modolell, 2010) visible, with Scer\GAL4^pnr-MD237 (Haenlin et al., 1997)
Phenotype Manifest In
	anterior dorsocentral bristle \| supernumerary, with Scer\GAL4^Bx-MS1096 (Zenvirt et al., 2008) cardioblast \| ectopic, with Scer\GAL4^twi.PB (Gajewski et al., 1999) cardioblast \| supernumerary, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Han and Olson, 2005) cardioblast \| supernumerary, with Scer\GAL4^tin.cBa (Reim and Frasch, 2005) cardiogenic mesoderm, with Scer\GAL4^Mef2.PR (Mandal et al., 2004) cardiogenic mesoderm, with Scer\GAL4^twi.PG (Mandal et al., 2004) denticle belt, with Scer\GAL4^Ubx.PdC (Herranz and Morata, 2001) dorsocentral bristle, with Scer\GAL4^pnr-MD237 (Ramain et al., 2000, Haenlin et al., 1997) dorsocentral bristle \| ectopic, with Scer\GAL4^ost-em462 (Calleja et al., 2000) dorsocentral bristle \| supernumerary, with Scer\GAL4^ap-md544 (Calleja et al., 2000) embryonic/larval dorsal vessel, with Scer\GAL4^twi.PB (Gajewski et al., 1999) embryonic/larval pericardial cell, with Scer\GAL4^twi.PB (Gajewski et al., 1999) embryonic/larval somatic muscle \| dorsal, with Scer\GAL4^twi.PB (Gajewski et al., 1999) embryonic/larval visceral muscle, with Scer\GAL4^twi.PB (Gajewski et al., 1999) embryonic epidermis, with Scer\GAL4^arm.PS (Herranz and Morata, 2001) embryonic epidermis, with Scer\GAL4^Ubx.PdC (Herranz and Morata, 2001) embryonic epidermis \| anterior compartment, with Scer\GAL4^wg-MD758 (Herranz and Morata, 2001) embryonic epidermis \| posterior compartment, with Scer\GAL4^en-e16E (Herranz and Morata, 2001) embryonic pericardial cell, with Scer\GAL4^Mef2.PR (Mandal et al., 2004) embryonic pericardial cell, with Scer\GAL4^twi.PG (Mandal et al., 2004) lymph gland primordium, with Scer\GAL4^Mef2.PR (Mandal et al., 2004) lymph gland primordium, with Scer\GAL4^twi.PG (Mandal et al., 2004) macrochaeta, with Scer\GAL4^C-765 (Garcia-Garcia et al., 1999) macrochaeta \| ectopic \| somatic clone, with Scer\GAL4^Ubx.PdC (Calleja et al., 2000) mesothoracic tergum \| cell autonomous \| ectopic \| somatic clone, with Scer\GAL4^Ubx.PdC (Calleja et al., 2000) mesothoracic tergum \| ectopic, with Scer\GAL4^ap-md544 (Calleja et al., 2000) mesothoracic tergum \| ectopic, with Scer\GAL4^bi-omb-Gal4 (Calleja et al., 2000) mesothoracic tergum \| ectopic, with Scer\GAL4^Bx-MS1096 (Calleja et al., 2000) mesothoracic tergum \| ectopic, with Scer\GAL4^C-765 (Calleja et al., 2000) mesothoracic tergum \| ectopic, with Scer\GAL4^nub-AC-62 (Calleja et al., 2000) mesothoracic tergum \| ectopic, with Scer\GAL4^vg.quadrant (Calleja et al., 2000) mesothoracic tergum \| lateral, with Scer\GAL4^ap-md544 (Calleja et al., 2000) mesothoracic tergum \| lateral \| somatic clone, with Scer\GAL4^Ubx.PdC (Calleja et al., 2000) microchaeta \| ectopic \| somatic clone, with Scer\GAL4^Ubx.PdC (Calleja et al., 2000) microchaeta \| supernumerary, with Scer\GAL4^ap-md544 (Calleja et al., 2000) posterior dorsocentral bristle \| supernumerary, with Scer\GAL4^Bx-MS1096 (Zenvirt et al., 2008) postnotum \| cell autonomous \| ectopic \| somatic clone, with Scer\GAL4^Ubx.PdC (Calleja et al., 2000) scutellar bristle \| supernumerary, with Scer\GAL4^pnr-MD237 (Calleja et al., 2000) scutum, with Scer\GAL4^C-765 (Garcia-Garcia et al., 1999) sensory mother cell \| ectopic, with Scer\GAL4^C-765 (de Navascués and Modolell, 2010) sternite, with Scer\GAL4^Dll-MD743 (Calleja et al., 2000) tergite \| ectopic, with Scer\GAL4^Dll-MD743 (Calleja et al., 2000) ventral nerve cord, with Scer\GAL4^Ubx.PdC (Herranz and Morata, 2001) wing, with Scer\GAL4^ap-md544 (Calleja et al., 2000) wing, with Scer\GAL4^bi-omb-Gal4 (Calleja et al., 2000) wing, with Scer\GAL4^Bx-MS1096 (Calleja et al., 2000) wing, with Scer\GAL4^C-765 (Calleja et al., 2000) wing, with Scer\GAL4^nub-AC-62 (Calleja et al., 2000) wing, with Scer\GAL4^vg.quadrant (Calleja et al., 2000) wing \| somatic clone, with Scer\GAL4^Ubx.PdC (Calleja et al., 2000)
Detailed Description
	Statement Reference Expression of pnr[Scer\UAS.cHa] under the control of Scer\GAL4[C-765] leads to the formation of ectopic sensory organ precursors. (de Navascués and Modolell, 2010) Expression of pnr[Scer\UAS.cHa] under the control of Scer\GAL4[Bx-MS1096] leads to excess dorsocentral bristles. (Zenvirt et al., 2008) Overexpression of pnr^Scer\UAS.cHa in the mesoderm of embryos, driven by both Scer\GAL4^how-24B and Scer\GAL4^twi.PG, induces the formation of extra cardioblasts. (Han and Olson, 2005) Expression of pnr^Scer\UAS.cHa in the dorsal mesoderm in stage 11-12 embryos, driven by Scer\GAL4^tin.cBa, results in the production of a few extra cardioblasts. (Reim and Frasch, 2005) Ectopic expression of pnr^Scer\UAS.cHa in the lateral domain of the cardiogenic mesoderm, driven by either Scer\GAL4^Mef2.PR or Scer\GAL4^twi.PG, causes a marked reduction of lymph-gland progenitors and pericardial cells in stage 16 embryos. (Mandal et al., 2004) Flies expressing pnr^Scer\UAS.cHa under the control of Scer\GAL4^pnr-MD237 are virtually wild type. The entire epidermis becomes dorsalised in embryos expressing pnr^Scer\UAS.cHa under the control of Scer\GAL4^arm.PS. The abdominal segments have a continuous belt of dorsal triangles around their circumference, indicating that the transformation is towards the mediodorsal pattern. Expression of pnr^Scer\UAS.cHa under the control of Scer\GAL4^Ubx.PdC results in transformation of the ventral epidermis into dorsal epidermis; the ventral denticles of most of the abdomen are replaced by dorsal spinules. Transformation from ventral to dorsal structures is also seen in segment T3 and the posterior part of T2 (the anterior part of T2 is the same as wild type). The ventral nerve cord is affected. The amnioserosa develops normally and germband retraction is normal. Expression of pnr^Scer\UAS.cHa under the control of Scer\GAL4^en-e16E results in transformation of ventral to dorsal epidermis in the P compartments. Expression of pnr^Scer\UAS.cHa under the control of Scer\GAL4^wg-MD758 results in transformation of ventral to dorsal epidermis in a portion of the anterior compartment of segments. The amnioserosa develops normally and germband retraction is normal in embryos expressing pnr^Scer\UAS.cHa under the control of Scer\GAL4^LP1. (Herranz and Morata, 2001) When expression of pnr^Scer\UAS.cHa is driven in clones by Scer\GAL4^Ubx.PdC in the lateral (LAT) region of the notum, they are eliminated or grow abnormally, in the medial (MED) region they grow almost normally. In clones that overlapped the regions, the cells situated in the MED region differentiated normally, in the LAT region, they tend to sort out from surrounding cells to form vesicles with sharp boundaries. pnr^Scer\UAS.cHa when driven by Scer\GAL4^pnr-MD237 produces no defects except supernumerary bristles in the scutellum. When driven by Scer\GAL4^ost-em462, there is an enlargement of the MED region as indicated by the increased number of dorsocentral bristles. When driven by Scer\GAL4^ap-md544, only structures that correspond to the MED region are formed, the LAT region does not develop. The MED pattern is normal except for an increase in the number of dorsocentral bristles and a higher density of microchaetae in the dorsocentral region. When expression of pnr^Scer\UAS.cHa is driven in clones by Scer\GAL4^Ubx.PdC in the wing, the clones develop structures typical of the notum with the characteristic trichome density of the notum as well as notal bristles. Notal structures are formed in a cell-autonomous manner. These wing to notum transformations appear anywhere in the wing, except the distal end, but are found preferentially in the more proximal regions. Clones induced during the larval first instar can build large portion of the notum pattern. Clones differentiating notal structures appear exclusively in the anterior compartment. In the posterior compartment clones differentiate patches of pigmented cuticle devoid of bristles interpreted as being postnotum. Expression of pnr^Scer\UAS.cHa by Scer\GAL4^ap-md544, Scer\GAL4^nub-AC-62, Scer\GAL4^Bx-MS1096, Scer\GAL4^C-765, Scer\GAL4^bi-omb-Gal4, or Scer\GAL4^vg.quadrant produce wing to notum transformations. Expression of pnr^Scer\UAS.cHa when driven by Scer\GAL4^MD743 leads top sternites that acquire pigmentation and bristle patterns similar to those of the tergites. In some cases the entire set of sternites are transformed into tergites, resulting in flies with an almost complete dorsoventral transformation of the abdomen. (Calleja et al., 2000) When pnr^Scer\UAS.cHa is driven by Scer\GAL4^pnr-MD237, an excess of dorsocentral bristles is seen. (Ramain et al., 2000) Embryos expressing pnr^Scer\UAS.cHa under the control of Scer\GAL4^twi.PB have an enlarged heart, due to the overspecification of cardial cells (both within the heart tube and throughout the dorsal region of the embryo). There is a decrease in the number of eve-expressing pericardial cells, and a decrease and abnormal organisation of dorsal body wall muscles. There is an apparent loss of cells within the visceral mesoderm lineage. (Gajewski et al., 1999) When expression is driven by Scer\GAL4^C-765, many macrochaetae develop in the region lateral to the usual dorsocentral (DC) area bristles. (Garcia-Garcia et al., 1999) Overexpression of pnr^Scer\UAS.cHa using Scer\GAL4^pnr-MD237 leads to an excess of dorsocentral bristles. (Haenlin et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^pnr-MD237, pnr^Scer\UAS.cHa has visible phenotype, enhanceable by Df(2L)ush^rev18 (Haenlin et al., 1997)
Other
Statement Reference Scer\GAL4^pnr-MD237, pnr^MD237, pnr^Scer\UAS.cHa, ppo^EP2478 has visible phenotype (Pena-Rangel et al., 2002) Scer\GAL4^pnr-MD237, pnr^Scer\UAS.cHa/pnr^MD237, ppo^EP2478 has visible phenotype (Pena-Rangel et al., 2002)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^pnr-MD237, pnr^Scer\UAS.cHa has dorsocentral bristle phenotype, enhanceable by Df(2L)ush^rev18 (Haenlin et al., 1997) Scer\GAL4^tin.cBa, pnr^Scer\UAS.cHa has cardioblast \| supernumerary phenotype, enhanceable by Doc2^Scer\UAS.cRa, Scer\GAL4^tin.cBa (Reim and Frasch, 2005) Scer\GAL4^tin.cBa, pnr^Scer\UAS.cHa has cardioblast \| supernumerary phenotype, enhanceable by Scer\GAL4^tin.cBa/tin^Scer\UAS.cYa (Reim and Frasch, 2005) Scer\GAL4^tin.cBa, pnr^Scer\UAS.cHa has cardioblast \| supernumerary phenotype, enhanceable by tin^Scer\UAS.cYa/Doc2^Scer\UAS.cRa, Scer\GAL4^tin.cBa (Reim and Frasch, 2005)
Enhancer of
Statement Reference pnr^Scer\UAS.cHa, Scer\GAL4^tin.cBa is an enhancer of cardioblast \| supernumerary phenotype of Doc2^Scer\UAS.cRa, Scer\GAL4^tin.cBa (Reim and Frasch, 2005) pnr^Scer\UAS.cHa/tin^Scer\UAS.cYa, Scer\GAL4^tin.cBa is an enhancer of cardioblast \| supernumerary phenotype of Doc2^Scer\UAS.cRa, Scer\GAL4^tin.cBa (Reim and Frasch, 2005) pnr^Scer\UAS.cHa/tin^Scer\UAS.cYa, Scer\GAL4^twi.2PE is an enhancer of cardioblast \| supernumerary phenotype of Doc2^Scer\UAS.cRa, Scer\GAL4^twi.2PE (Reim and Frasch, 2005)
Suppressor of
Statement Reference pnr^Scer\UAS.cHa/Scer\GAL4^pnr-MD237 is a suppressor of thorax phenotype of Chi^E (Ramain et al., 2000)
NOT Suppressor of
Statement Reference pnr^Scer\UAS.cHa/Scer\GAL4^pnr-MD237 is a non-suppressor of dorsocentral bristle phenotype of Chi^E (Ramain et al., 2000)
Other
Statement Reference Scer\GAL4^pnr-MD237, pnr^MD237, pnr^Scer\UAS.cHa, ppo^EP2478 has chaeta phenotype (Pena-Rangel et al., 2002) Scer\GAL4^pnr-MD237, pnr^MD237, pnr^Scer\UAS.cHa, ppo^EP2478 has scutellum phenotype (Pena-Rangel et al., 2002) Scer\GAL4^pnr-MD237, pnr^Scer\UAS.cHa/pnr^MD237, ppo^EP2478 has chaeta phenotype (Pena-Rangel et al., 2002) Scer\GAL4^pnr-MD237, pnr^Scer\UAS.cHa/pnr^MD237, ppo^EP2478 has scutellum phenotype (Pena-Rangel et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference Expression of pnr^Scer\UAS.cHa, under the control of Scer\GAL4^tin.cBa, in a Df(3L)DocA background provides a moderate rescue of cardioblast formation. Coexpression of pnr^Scer\UAS.cHa and tin^Scer\UAS.cYa, under the control of Scer\GAL4^tin.cBa, induces the formation of more cardioblasts than expression of pnr^Scer\UAS.cHa alone. When Doc2^Scer\UAS.cRa is coexpressed with only pnr^Scer\UAS.cHa, or pnr^Scer\UAS.cHa and tin^Scer\UAS.cYa together, large numbers of extra cardioblasts are formed. Expression of all three transgenes results in double the amount of cardioblasts compared to wild type. Coexpression of Doc2^Scer\UAS.cRa, tin^Scer\UAS.cYa, and pnr^Scer\UAS.cHa, under the control of Scer\GAL4^twi.2PE, causes widespread ectopic cardioblast formation to a much greater extent than expression of Doc2^Scer\UAS.cRa alone or in combination with tin^Scer\UAS.cYa plus pnr^Scer\UAS.cHa. (Reim and Frasch, 2005) Flies expressing both pnr^Scer\UAS.cHa and ppo^EP2478 under the control of Scer\GAL4^ap-md544 in a pnr^MD237/+ background show a reduction in thoracic bristles and an altered scutellum. (Pena-Rangel et al., 2002) The addition of overexpressed pnr^Scer\UAS.cHa (when driven by Scer\GAL4^pnr-MD237) suppresses the cleft phenotype of Chi^E mutants. (Ramain et al., 2000) The dorsocentral bristle phenotype seen in flies expressing pnr^Scer\UAS.cHa using Scer\GAL4^pnr-MD237 is enhanced by Df(2L)ush^rev18. (Haenlin et al., 1997)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	pnr^Scer\UAS.cHa, Scer\GAL4^how-24B, Scer\GAL4^how-24B partially rescues pnr^VX6 (Qian and Bodmer, 2009) pnr^Scer\UAS.cHa, Scer\GAL4^twi.PG, Scer\GAL4^how-24B, Scer\GAL4^how-24B partially rescues pnr¹ (Qian and Bodmer, 2009) pnr^Scer\UAS.cHa, Scer\GAL4^twi.PG, Scer\GAL4^how-24B, Scer\GAL4^how-24B partially rescues pnr^VX6 (Klinedinst and Bodmer, 2003, Qian and Bodmer, 2009) pnr^Scer\UAS.cHa/Scer\GAL4^da.G32 partially rescues pnr^VX6 (Klinedinst and Bodmer, 2003) pnr^Scer\UAS.cHa/Scer\GAL4^Mef2.PR partially rescues pnr¹ (Qian and Bodmer, 2009) pnr^Scer\UAS.cHa/Scer\GAL4^Mef2.PR partially rescues pnr^VX6 (Qian and Bodmer, 2009) pnr^Scer\UAS.cHa/Scer\GAL4^tin.CΔ4 partially rescues pnr¹ (Qian and Bodmer, 2009) pnr^Scer\UAS.cHa/Scer\GAL4^tin.CΔ4 partially rescues pnr^VX6 (Qian and Bodmer, 2009) Scer\GAL4^zen.Kr.PF/pnr^Scer\UAS.cHa partially rescues pnr^VX6 (Klinedinst and Bodmer, 2003)
Comments	The increased arrest/fibrillation rate of pnr[1]/+ and pnr[VX6]/+ flies that is seen upon electrical pacing of the heart is partially rescued by expression of pnr[Scer\UAS.cHa] under the control of Scer\GAL4[Mef2.PR]. The increased arrest/fibrillation rate of pnr[1]/+ and pnr[VX6]/+ flies that is seen upon electrical pacing of the heart is partially rescued by expression of pnr[Scer\UAS.cHa] under the control of Scer\GAL4[tin.CΔ4] (rescue of the phenotype is seen in 1 and 3 week old flies, but not in 5 week old flies). The increased arrest/fibrillation rate of pnr[1]/+ and pnr[VX6]/+ flies that is seen upon electrical pacing of the heart is partially rescued by expression of pnr[Scer\UAS.cHa] under the simultaneous control of both Scer\GAL4[twi.PG] and Scer\GAL4[how-24B] (rescue of the phenotype is seen in 1 and 3 week old flies, but not in 5 week old flies). The decreased lifespan of pnr[VX6]/+ flies can be partially rescued by expression of pnr[Scer\UAS.cHa] under the simultaneous control of both Scer\GAL4[twi.PG] and Scer\GAL4[how-24B]. The decreased lifespan of pnr[VX6]/+ flies can be partially rescued by expression of pnr[Scer\UAS.cHa] under the control of either Scer\GAL4[Mef2.PR] or Scer\GAL4[how-24B]. The arrhythmias and slow heart beats of pnr[VX6]/+ flies can be rescued by expression of pnr[Scer\UAS.cHa] under the control of either Scer\GAL4[Mef2.PR] or Scer\GAL4[tin.CΔ4]. The arrhythmias and slow heart beats of pnr[VX6]/+ flies can be rescued by expression of pnr[Scer\UAS.cHa] under the simultaneous control of both Scer\GAL4[twi.PG] and Scer\GAL4[how-24B]. (Qian and Bodmer, 2009) When pnr^Scer\UAS.cHa is driven by Scer\GAL4^how-24B and Scer\GAL4^twi.PG in pnr^VX6 embryos, 57% of embryos show a cardiac-specific tin expression that is restored close to wild-type levels. A similar level of rescue is seen when pnr^Scer\UAS.cHa is driven by Scer\GAL4^da.G32. A small amount of rescue is also seen if pnr^Scer\UAS.cHa is driven by Scer\GAL4^zen.Kr.PF in the mesoderm. (Klinedinst and Bodmer, 2003)
Stocks ( 1 )
Bloomington	7223 w^*; P{UAS-pnr.H}13/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	pnr^Scer\UAS.cHa pnr^UAS.cHa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Haenlin (Haenlin et al., 1997)
Secondary FlyBase IDs

References ( 28 )

Generate a list of	All references relating to this allele ( 28 )

List References by type	Research paper ( 27 ) Supplementary material ( 1 )
Recent research papers ( 1 )
	Ryu et al., 2011, Dev. Dyn. 240(1): 86--96 Tinman is a direct activator of midline in the drosophila dorsal vessel. [FBrf0212547] Show all research papers ...

Allele Dmel\pnrScer\UAS.cHa

Allele Dmel\pnr^Scer\UAS.cHa