|Name||Saccharomyces cerevisiae UAS construct a of Jackson||FlyBase ID||FBal0083022|
|Feature type||allele||Associated gene||Dmel\dally|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
(Crickmore and Mann, 2007, Tsuda et al., 1999, Fox and Zinn, 2005, Fujise et al., 2003, Takeo et al., 2005, de Navas et al., 2006, Kirkpatrick et al., 2006, Giraldez et al., 2002, Shulman and Feany, 2003, Strigini and Cohen, 2000, Jackson et al., 1997, Avanesov et al., 2012, Makhijani et al., 2007, Guo and Wang, 2009, Kleinschmit et al., 2010, Xia et al., 2012)
|Phenotype Manifest In|
Expression of dally[Scer\UAS.cJa] under the control of Scer\GAL4[nub-AC-62] does not induce wing margin scalloping but has weak effects on margin bristle number.
Expression of dally[Scer\UAS.cJa] in the inner germarium sheath (IGS) cells under the control of Scer\GAL4[C587] results in tumorous germaria that are filled with bam-negative GSC-like cells.
Expression of dally[Scer\UAS.cJa] under the control of Scer\GAL4[C587], Scer\GAL4[ptc-559.1] or Scer\GAL4[nos.UTR.T:Hsim\VP16] results in the progressive accumulation of extra germline stem cell-like cells (the cells have spherical fusomes) in the ovarioles. The extra germline stem cell-like cells hardly differentiate in females expressing dally[Scer\UAS.cJa] under the control of Scer\GAL4[C587], leaving large stage gaps between the germarium and the adjacent egg chamber. BrdU incorporation is seen in multiple two-cell clusters posterior and distant from the niche in germaria expressing dally[Scer\UAS.cJa] under the control of Scer\GAL4[C587] (this is never seen in wild type). Expression of dally[Scer\UAS.cJa] under the control of Scer\GAL4[bab1-Pgal4-2] results in the progressive accumulation of some extra germline stem cell-like cells in the ovarioles. Expression of dally[Scer\UAS.cJa] under the control of Scer\GAL4[en-e16E] has no effect on germline stem cell number in the female germaria.
Expression of dallyScer\UAS.cJa under the control of Scer\GAL4en-e16E increases the posterior:anterior size ratio of the appendage-forming region of the haltere disc from the wild-type ratio of 0.48 to 0.59. The overall size of the adult haltere is 5% larger in animals of this genotype than in controls.
The posterior compartment/anterior compartment width ratio is increased in the haltere pouches of larvae expressing dallyScer\UAS.cJa under the control of Scer\GAL4en-e16E compared to control discs.
Expression of dallyScer\UAS.cJa in the wing, under the control of Scer\GAL4en-e16E, results in enlargement of the posterior portion by 46%, and enlargement of the anterior portion by a more modest 13%. In addition, the formation of the posterior and anterior crossveins are disrupted in these wings. Expression of dallyScer\UAS.cJa in the eye, driven by Scer\GAL4ey.PH, causes an eye overgrowth phenotype. Ommatidia in these eyes are misshapen, with a reduced number of photoreceptors per ommatidial unit. Expression of dallyScer\UAS.cJa under the control of Scer\GAL4A9 in a dallygem/dallyΔP-527 or dallygem mutant background results in the formation of excess dorsocentral bristles (the excess is around 30% compared to wild-type wings).
Expression of dallyScer\UAS.cJa, under the control of both Scer\GAL4how-24B and Scer\GAL4G14, produces SNa bifurcation phenotypes at a low penetrance (~11%).
|Phenotype Manifest In|
dallyScer\UAS.cJa, Scer\GAL4nub-AC-62 is an enhancer of wing margin bristle phenotype of Drer\wif1Scer\UAS.cAa, Scer\GAL4nub-AC-62
The reduction in the number of photoreceptors per ommatidia seen in eyes that express dallyScer\UAS.cJa under the control of Scer\GAL4ey.PH is suppressed in a Madunspecified/+ mutant background.
Expression of dally[Scer\UAS.cJa] synergistically enhances the wing defects seen when Drer\wif1[Scer\UAS.cAa] is expressed under the control of Scer\GAL4[nub-AC-62]. Wing margin bristles and the first longitudinal vein are almost completely eliminated. Wing size is further reduced.
|Complementation & Rescue Data|
Expression of dally[Scer\UAS.cJa] under the control of Scer\GAL4[bab1-Pgal4-2], Scer\GAL4[C587] or Scer\GAL4[en-e16E] partially rescues the loss of germline stem cells seen in homozygous dally[gem] ovarioles, with expression driven by Scer\GAL4[bab1-Pgal4-2] providing greater rescue.
|Stocks ( 1 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Saccharomyces cerevisiae UAS construct a of Jackson
|Secondary FlyBase IDs|
|References ( 17 )|