Allele Dmel\dally^Scer\UAS.cJa

General Information
Symbol	Dmel\dally^Scer\UAS.cJa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Jackson	FlyBase ID	FBal0083022
Feature type	allele	Associated gene	Dmel\dally

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Jackson et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: dally cDNA is expressed under the control of Scer\UAS regulatory sequences. (Jackson et al., 1997)
Carried in construct	P{UAS-dally.J} (Crickmore and Mann, 2007, Tsuda et al., 1999, Fox and Zinn, 2005, Fujise et al., 2003, Takeo et al., 2005, de Navas et al., 2006, Kirkpatrick et al., 2006, Giraldez et al., 2002, Shulman and Feany, 2003, Strigini and Cohen, 2000, Jackson et al., 1997, Avanesov et al., 2012, Makhijani et al., 2007, Guo and Wang, 2009, Kleinschmit et al., 2010, Xia et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	hyperplasia \| adult stage, with Scer\GAL4^C587 (Xia et al., 2012) lethal, with Scer\GAL4^unspecified (Tsuda et al., 1999) neuroanatomy defective, with Scer\GAL4^G14, Scer\GAL4^how-24B (Fox and Zinn, 2005)
Phenotype Manifest In
	abdominal segmental nerve, with Scer\GAL4^G14, Scer\GAL4^how-24B (Fox and Zinn, 2005) anterior crossvein, with Scer\GAL4^en-e16E (Kirkpatrick et al., 2006) dorsocentral bristle \| supernumerary, with Scer\GAL4^A9, dally^gem (Kirkpatrick et al., 2006) dorsocentral bristle \| supernumerary, with Scer\GAL4^A9, dally^ΔP-527/dally^gem (Kirkpatrick et al., 2006) embryonic epidermis, with Scer\GAL4^en-e16E (Tsuda et al., 1999) embryonic epidermis, with Scer\GAL4^prd.RG1 (Tsuda et al., 1999) eye, with Scer\GAL4^ey.PH (Kirkpatrick et al., 2006) eye photoreceptor cell, with Scer\GAL4^ey.PH (Kirkpatrick et al., 2006) female germline cell \| supernumerary, with Scer\GAL4^C587 (Xia et al., 2012) female germline stem cell \| supernumerary, with Scer\GAL4^bab1-Pgal4-2 (Guo and Wang, 2009) female germline stem cell \| supernumerary, with Scer\GAL4^C587 (Guo and Wang, 2009) female germline stem cell \| supernumerary, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Guo and Wang, 2009) female germline stem cell \| supernumerary, with Scer\GAL4^ptc-559.1 (Guo and Wang, 2009) germarium, with Scer\GAL4^C587 (Xia et al., 2012) haltere, with Scer\GAL4^en-e16E (Crickmore and Mann, 2007) haltere disc, with Scer\GAL4^en-e16E (Crickmore and Mann, 2007) haltere disc \| posterior compartment, with Scer\GAL4^en-e16E (de Navas et al., 2006) ommatidium, with Scer\GAL4^ey.PH (Kirkpatrick et al., 2006) ovary, with Scer\GAL4^C587 (Xia et al., 2012) posterior crossvein, with Scer\GAL4^en-e16E (Kirkpatrick et al., 2006) wing, with Scer\GAL4^nub-AC-62 (Avanesov et al., 2012) wing \| anterior compartment, with Scer\GAL4^en-e16E (Kirkpatrick et al., 2006) wing \| posterior compartment, with Scer\GAL4^en-e16E (Kirkpatrick et al., 2006) wing margin bristle, with Scer\GAL4^nub-AC-62 (Avanesov et al., 2012)
Detailed Description
	Statement Reference Expression of dally[Scer\UAS.cJa] under the control of Scer\GAL4[nub-AC-62] does not induce wing margin scalloping but has weak effects on margin bristle number. (Avanesov et al., 2012) Expression of dally[Scer\UAS.cJa] in the inner germarium sheath (IGS) cells under the control of Scer\GAL4[C587] results in tumorous germaria that are filled with bam-negative GSC-like cells. (Xia et al., 2012) Expression of dally[Scer\UAS.cJa] under the control of Scer\GAL4[C587], Scer\GAL4[ptc-559.1] or Scer\GAL4[nos.UTR.T:Hsim\VP16] results in the progressive accumulation of extra germline stem cell-like cells (the cells have spherical fusomes) in the ovarioles. The extra germline stem cell-like cells hardly differentiate in females expressing dally[Scer\UAS.cJa] under the control of Scer\GAL4[C587], leaving large stage gaps between the germarium and the adjacent egg chamber. BrdU incorporation is seen in multiple two-cell clusters posterior and distant from the niche in germaria expressing dally[Scer\UAS.cJa] under the control of Scer\GAL4[C587] (this is never seen in wild type). Expression of dally[Scer\UAS.cJa] under the control of Scer\GAL4[bab1-Pgal4-2] results in the progressive accumulation of some extra germline stem cell-like cells in the ovarioles. Expression of dally[Scer\UAS.cJa] under the control of Scer\GAL4[en-e16E] has no effect on germline stem cell number in the female germaria. (Guo and Wang, 2009) Expression of dally^Scer\UAS.cJa under the control of Scer\GAL4^en-e16E increases the posterior:anterior size ratio of the appendage-forming region of the haltere disc from the wild-type ratio of 0.48 to 0.59. The overall size of the adult haltere is 5% larger in animals of this genotype than in controls. (Crickmore and Mann, 2007) The posterior compartment/anterior compartment width ratio is increased in the haltere pouches of larvae expressing dally^Scer\UAS.cJa under the control of Scer\GAL4^en-e16E compared to control discs. (de Navas et al., 2006) Expression of dally^Scer\UAS.cJa in the wing, under the control of Scer\GAL4^en-e16E, results in enlargement of the posterior portion by 46%, and enlargement of the anterior portion by a more modest 13%. In addition, the formation of the posterior and anterior crossveins are disrupted in these wings. Expression of dally^Scer\UAS.cJa in the eye, driven by Scer\GAL4^ey.PH, causes an eye overgrowth phenotype. Ommatidia in these eyes are misshapen, with a reduced number of photoreceptors per ommatidial unit. Expression of dally^Scer\UAS.cJa under the control of Scer\GAL4^A9 in a dally^gem/dally^ΔP-527 or dally^gem mutant background results in the formation of excess dorsocentral bristles (the excess is around 30% compared to wild-type wings). (Kirkpatrick et al., 2006) Expression of dally^Scer\UAS.cJa, under the control of both Scer\GAL4^how-24B and Scer\GAL4^G14, produces SNa bifurcation phenotypes at a low penetrance (~11%). (Fox and Zinn, 2005) Expression of dally^Scer\UAS.cJa driven by Scer\GAL4^en-e16E or Scer\GAL4^prd.RG1 produces cuticle defects consistent with an increase in the level of wg activity. High levels of expression are lethal and few embryos survive to produce cuticle. (Tsuda et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Scer\GAL4[-], dally^Scer\UAS.cJa, hh^Mrt has visible \| dominant phenotype (Jackson et al., 1997) Scer\GAL4[-], dally^Scer\UAS.cJa, hh^Mrt has visible phenotype (Jackson et al., 1997)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^ey.PH, dally^Scer\UAS.cJa has eye photoreceptor cell phenotype, suppressible by Mad[+]/Mad^unspecified (Kirkpatrick et al., 2006)
Enhancer of
Statement Reference dally^Scer\UAS.cJa, Scer\GAL4^nub-AC-62 is an enhancer of wing margin bristle phenotype of Drer\wif1^Scer\UAS.cAa, Scer\GAL4^nub-AC-62 (Avanesov et al., 2012) dally^Scer\UAS.cJa, Scer\GAL4^nub-AC-62 is an enhancer of wing phenotype of Drer\wif1^Scer\UAS.cAa, Scer\GAL4^nub-AC-62 (Avanesov et al., 2012)
Other
Statement Reference Scer\GAL4[-], dally^Scer\UAS.cJa, hh^Mrt has wing \| anterior phenotype (Jackson et al., 1997, Jackson et al., 1997)
Additional Comments
Genetic Interactions
Statement Reference The reduction in the number of photoreceptors per ommatidia seen in eyes that express dally^Scer\UAS.cJa under the control of Scer\GAL4^ey.PH is suppressed in a Mad^unspecified/+ mutant background. (Kirkpatrick et al., 2006) Potentiates the hh^Mrt/+ phenotype, converting the wing overgrowth phenotype to mirror image wing duplications in hh^Mrt/+ ; dally^Scer\UAS.cJa flies. This phenotype is seen in the absence of a Scer\GAL4 activator in a number of different P{UAS-dally.J} insertion lines. (Jackson et al., 1997)
Xenogenetic Interactions
Statement Reference Expression of dally[Scer\UAS.cJa] synergistically enhances the wing defects seen when Drer\wif1[Scer\UAS.cAa] is expressed under the control of Scer\GAL4[nub-AC-62]. Wing margin bristles and the first longitudinal vein are almost completely eliminated. Wing size is further reduced. (Avanesov et al., 2012)
Complementation & Rescue Data
Rescues	dally^Scer\UAS.cJa/Scer\GAL4^A9 rescues dally^gem (Kirkpatrick et al., 2006) dally^Scer\UAS.cJa/Scer\GAL4^A9 rescues Df(3L)Scf-R6/dally^gem (Kirkpatrick et al., 2006)
Partially rescues	dally^Scer\UAS.cJa/Scer\GAL4^bab1-Pgal4-2 partially rescues dally^gem (Guo and Wang, 2009) dally^Scer\UAS.cJa/Scer\GAL4^C587 partially rescues dally^gem (Guo and Wang, 2009) dally^Scer\UAS.cJa/Scer\GAL4^en-e16E partially rescues dally^gem (Guo and Wang, 2009)
Comments	Expression of dally[Scer\UAS.cJa] under the control of Scer\GAL4[bab1-Pgal4-2], Scer\GAL4[C587] or Scer\GAL4[en-e16E] partially rescues the loss of germline stem cells seen in homozygous dally[gem] ovarioles, with expression driven by Scer\GAL4[bab1-Pgal4-2] providing greater rescue. (Guo and Wang, 2009)
Stocks ( 1 )
Bloomington	5397 w^* P{UAS-dally.J}SJ1
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	dally^Scer\UAS.cJa dally^UAS.cJa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Jackson (Jackson et al., 1997)
Secondary FlyBase IDs

References ( 17 )
Research paper	Avanesov et al., 2012, PLoS Genet. 8(2): e1002503 The Role of Glypicans in Wnt Inhibitory Factor-1 Activity and the Structural Basis of Wif1's Effects on Wnt and Hedgehog Signaling. [FBrf0217590] Xia et al., 2012, Curr. Biol. 22(6): 515--521 The Niche-Dependent Feedback Loop Generates a BMP Activity Gradient to Determine the Germline Stem Cell Fate. [FBrf0217877] Kleinschmit et al., 2010, Dev. Biol. 345(2): 204--214 Drosophila heparan sulfate 6-O endosulfatase regulates Wingless morphogen gradient formation. [FBrf0211640] Guo and Wang, 2009, Development 136(21): 3627--3635 The glypican Dally is required in the niche for the maintenance of germline stem cells and short-range BMP signaling in the Drosophila ovary. [FBrf0208956] Crickmore and Mann, 2007, Development 134(2): 327--334 Hox control of morphogen mobility and organ development through regulation of glypican expression. [FBrf0194329] Makhijani et al., 2007, Dev. Biol. 302(1): 243--255 Modulation of Decapentaplegic gradient during haltere specification in Drosophila. [FBrf0193834] de Navas et al., 2006, Development 133(22): 4495--4506 The Ultrabithorax Hox gene of Drosophila controls haltere size by regulating the Dpp pathway. [FBrf0194425] Kirkpatrick et al., 2006, Dev. Biol. 300(2): 570--582 The function of a Drosophila glypican does not depend entirely on heparan sulfate modification. [FBrf0194660] Han et al., 2005, Development 132(4): 667--679 Drosophila glypicans Dally and Dally-like shape the extracellular Wingless morphogen gradient in the wing disc. [FBrf0183936] Takeo et al., 2005, Dev. Biol. 284(1): 204--218 Expression of a secreted form of Dally, a Drosophila glypican, induces overgrowth phenotype by affecting action range of Hedgehog. [FBrf0187400] Fujise et al., 2003, Development 130(8): 1515--1522 Dally regulates Dpp morphogen gradient formation in the Drosophila wing. [FBrf0158874] Shulman and Feany, 2003, Genetics 165(3): 1233--1242 Genetic modifiers of tauopathy in Drosophila. [FBrf0167629] Giraldez et al., 2002, Dev. Cell 2(5): 667--676 HSPG modification by the secreted enzyme Notum shapes the Wingless morphogen gradient. [FBrf0148946] Strigini and Cohen, 2000, Curr. Biol. 10(6): 293--300 Wingless gradient formation in the Drosophila wing. [FBrf0127338] Tsuda et al., 1999, Nature 400(6741): 276--280 The cell-surface proteoglycan Dally regulates Wingless signalling in Drosophila. [FBrf0110960] Jackson et al., 1997, Development 124(20): 4113--4120 dally, a Drosophila glypican, controls cellular responses to the TGF-beta-related morphogen, Dpp. [FBrf0099288]
Supplementary material	Fox and Zinn, 2005, Curr. Biol. 15(19): Supplemental data. [FBrf0191745]

Allele Dmel\dallyScer\UAS.cJa

Allele Dmel\dally^Scer\UAS.cJa