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General Information
Symbol
Dmel\dallyUAS.cJa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Jackson
FlyBase ID
FBal0083022
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dally, UAS-dally+
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
dally cDNA is expressed under the control of UASt regulatory sequences.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Expression of dallyScer\UAS.cJa under the control of Scer\GAL4nub-AC-62 does not induce wing margin scalloping but has weak effects on margin bristle number.
Embryos expressing dallyScer\UAS.cJa under the control of Scer\GAL4elav.PU show ISNb motor axon guidance defects (53.8% of hemisegments) and SNa motor axon guidance defects (25.5% of hemisegments).
Expression of dallyScer\UAS.cJa in the inner germarium sheath (IGS) cells under the control of Scer\GAL4C587 results in tumorous germaria that are filled with bam-negative GSC-like cells.
Expression of dallyScer\UAS.cJa under the control of Scer\GAL4C587, Scer\GAL4ptc-559.1 or Scer\GAL4nos.UTR.T:Hsim\VP16 results in the progressive accumulation of extra germline stem cell-like cells (the cells have spherical fusomes) in the ovarioles. The extra germline stem cell-like cells hardly differentiate in females expressing dallyScer\UAS.cJa under the control of Scer\GAL4C587, leaving large stage gaps between the germarium and the adjacent egg chamber. BrdU incorporation is seen in multiple two-cell clusters posterior and distant from the niche in germaria expressing dallyScer\UAS.cJa under the control of Scer\GAL4C587 (this is never seen in wild type). Expression of dallyScer\UAS.cJa under the control of Scer\GAL4bab1-Pgal4-2 results in the progressive accumulation of some extra germline stem cell-like cells in the ovarioles. Expression of dallyScer\UAS.cJa under the control of Scer\GAL4en-e16E has no effect on germline stem cell number in the female germaria.
Expression of dallyScer\UAS.cJa under the control of Scer\GAL4en-e16E increases the posterior:anterior size ratio of the appendage-forming region of the haltere disc from the wild-type ratio of 0.48 to 0.59. The overall size of the adult haltere is 5% larger in animals of this genotype than in controls.
Expression of dallyScer\UAS.cJa in the wing, under the control of Scer\GAL4en-e16E, results in enlargement of the posterior portion by 46%, and enlargement of the anterior portion by a more modest 13%. In addition, the formation of the posterior and anterior crossveins are disrupted in these wings. Expression of dallyScer\UAS.cJa in the eye, driven by Scer\GAL4ey.PH, causes an eye overgrowth phenotype. Ommatidia in these eyes are misshapen, with a reduced number of photoreceptors per ommatidial unit. Expression of dallyScer\UAS.cJa under the control of Scer\GAL4A9 in a dallygem/dallyΔP-527 or dallygem mutant background results in the formation of excess dorsocentral bristles (the excess is around 30% compared to wild-type wings).
The posterior compartment/anterior compartment width ratio is increased in the haltere pouches of larvae expressing dallyScer\UAS.cJa under the control of Scer\GAL4en-e16E compared to control discs.
Expression of dallyScer\UAS.cJa, under the control of both Scer\GAL4how-24B and Scer\GAL4G14, produces SNa bifurcation phenotypes at a low penetrance (~11%).
Expression of dallyScer\UAS.cJa driven by Scer\GAL4en-e16E or Scer\GAL4prd.RG1 produces cuticle defects consistent with an increase in the level of wg activity. High levels of expression are lethal and few embryos survive to produce cuticle.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement
Reference
Scer\GAL4[-], dallyUAS.cJa, hhMrt has visible phenotype
Scer\GAL4[-], dallyUAS.cJa, hhMrt has visible | dominant phenotype
Phenotype Manifest In
Suppressed by
Statement
Reference
Enhancer of
NOT Suppressor of
Other
Statement
Reference
Scer\GAL4[-], dallyUAS.cJa, hhMrt has wing | anterior phenotype
Additional Comments
Genetic Interactions
Statement
Reference
Co-expression of dallyScer\UAS.cJa with enokGD4037 and enokHM05195 under the control of Scer\GAL4bab1-Gal4 and Scer\GAL80ts.αTub84B is unable to attenuate the decline in germline stem cell numbers.
The reduction in the number of photoreceptors per ommatidia seen in eyes that express dallyScer\UAS.cJa under the control of Scer\GAL4ey.PH is suppressed in a Madunspecified/+ mutant background.
Potentiates the hhMrt/+ phenotype, converting the wing overgrowth phenotype to mirror image wing duplications in hhMrt/+ ; dallyScer\UAS.cJa flies. This phenotype is seen in the absence of a Scer\GAL4 activator in a number of different P{UAS-dally.J} insertion lines.
Xenogenetic Interactions
Statement
Reference
Expression of dallyScer\UAS.cJa synergistically enhances the wing defects seen when Drer\wif1Scer\UAS.cAa is expressed under the control of Scer\GAL4nub-AC-62. Wing margin bristles and the first longitudinal vein are almost completely eliminated. Wing size is further reduced.
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
dallyScer\UAS.cJa
dallyUAS.cJa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Jackson
Secondary FlyBase IDs
    References (23)