|Name||Saccharomyces cerevisiae UAS construct a of Bonini||FlyBase ID||FBal0083045|
|Feature type||allele||Associated gene||Dmel\eya|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
(Bonini et al., 1997, Zimmerman et al., 2000, Medioni and Noselli, 2005, Warrick et al., 1999, Pauli et al., 2005, Katsuyama et al., 2005, Anderson et al., 2006, Bai and Montell, 2002, Curtiss and Mlodzik, 2000, Bui et al., 2000, Bui et al., 2000, Seimiya and Gehring, 2000, Czerny et al., 1999, Clark et al., 2006, Blanco et al., 2010, Salzer et al., 2010)
|Phenotype Manifest In|
Overexpression of eyaScer\UAS.cBa using Scer\GAL4upd blocks anterior polar cell differentiation in egg chambers. As a consequence, the apical cap, which is formed from basement membrane components, that covers anterior polar cells in wild-type egg chambers is not present.
Expression of eyaScer\UAS.cBa under the control of Scer\GAL4hs.PB results in compound egg chambers which are not separated by interfollicular stalks and which are not always completely enveloped by the somatic epithelial layer. No germ cells are connected to each other by more than four ring canals indicating that there has not been an extra round of cell division. Expression of eyaScer\UAS.cBa under the control of Scer\GAL4upd results in one-third of egg chambers lacking polar cells at one end.
Expression of eyaScer\UAS.cBa driven by Scer\GAL4dpp.blk1 causes ectopic retinal development, leading to the eventual formation of ectopic compound eyes in the adult.
Expression of eyaScer\UAS.cBa under the control of Scer\GAL4ey.PH results in flies with smaller eyes than normal (which contain fewer facets than wild-type eyes). The regular arrangement of facets is lost in these eyes. Expression of eyaScer\UAS.cBa under the control of Scer\GAL4dpp.blk1 results in the induction of ectopic eyes.
Ectopic eyes are produced at a frequency of 10% if eyaScer\UAS.cBa is expressed under the control of Scer\GAL4dpp.blk1.
10% of flies carrying a single copy of eyaScer\UAS.cBa expressed under the control of a single copy of Scer\GAL4dpp.blk1 in a wild-type background have ectopic eyes on the antennal segment which resemble normal eyes. These ectopic eyes contain photoreceptor cells in a pattern similar to that of the normal compound eye. 96% of flies carrying two copies of eyaScer\UAS.cBa expressed under the control of two copies of Scer\GAL4dpp.blk1 in a wild-type background have ectopic eyes on the antennae. 80% of these flies also show ommatidial formation on the legs, and occasionally on the wings.
|NOT Suppressor of|
|Phenotype Manifest In|
Scer\GAL4dpp.blk1/eyaScer\UAS.cBa is an enhancer of antenna phenotype of Scer\GAL4dpp.blk1, eyScer\UAS.cHa
Scer\GAL4dpp.blk1/eyaScer\UAS.cBa is an enhancer of haltere phenotype of Scer\GAL4dpp.blk1, eyScer\UAS.cHa
Scer\GAL4dpp.blk1/eyaScer\UAS.cBa is an enhancer of leg phenotype of Scer\GAL4dpp.blk1, eyScer\UAS.cHa
Scer\GAL4dpp.blk1/eyaScer\UAS.cBa is an enhancer of proboscis phenotype of Scer\GAL4dpp.blk1, eyScer\UAS.cHa
Scer\GAL4dpp.blk1/eyaScer\UAS.cBa is a suppressor | partially of morphogenetic furrow phenotype of dppd-blk
Scer\GAL4dpp.blk1/eyaScer\UAS.cBa is a suppressor | somatic clone of eye disc | posterior | somatic clone phenotype of Mad1-2
|NOT Suppressor of|
Co-expression of eyaScer\UAS.cBa and Six4Scer\UAS.cCa in the twi domain, under the control of Scer\GAL4twi.PG strongly affects the mesoderm. Several cell types derived from the ventral and dorsolateral mesoderm expand. Greatly increased numbers of somatic gonadal precursors results in an enlarged gonad. There are also more fat body precursors, and these now extend into the dorsal region of the mesoderm, suggesting that dorsal cells have taken on more ventral fates. In contrast, derivatives of the dorsal mesoderm are disrupted. In stage 17 embryos no myosin-expressing cardioblasts are visible, while there are gaps in the pattern of dorsal muscles. Similarly, there are gaps in the visceral mesoderm at stage 12. In the ventral region of the somatic musculature, there are duplications of the ventral acute muscle (VA3), while the pattern of ventral oblique muscles is disrupted. The lateral somatic musculature also appears larger.
Expression of eyaScer\UAS.cBa and soScer\UAS.cPa driven by Scer\GAL4dpp.blk1 causes ectopic retinal development, leading to the eventual formation of ectopic compound eyes in the adult.
Co-expression of eyaScer\UAS.cBa and soScer\UAS.cPa under the control of Scer\GAL4dpp.blk1 results in ectopic eye tissue at many sites, including the antenna, legs and wings.
The dppd-blk phenotype is partially rescued by eyaScer\UAS.cBa expressed under the control of Scer\GAL4dpp.blk1. The small size of dppd-blk/dppd12 eye discs is partially rescued by eyaScer\UAS.cBa expressed under the control of Scer\GAL4dpp.blk1 and greater numbers of developing ommatidia are seen. Eye differentiation is rescued in Mad1-2 clones that touch the posterior margin of the eye disc when eyaScer\UAS.cBa is expressed under the control of Scer\GAL4dpp.blk1.
Ectopic eyes are produced at a frequency of 60% if eyaScer\UAS.cBa and soScer\UAS.cPa are coexpressed under the control of Scer\GAL4dpp.blk1.
Ectopic eye formation in flies expressing eyScer\UAS.cHa under the control of Scer\GAL4dpp.blk1 is greatly enhanced if the flies also carry eyaScer\UAS.cBa; the ectopic eyes formed in flies expressing both eyScer\UAS.cHa and eyaScer\UAS.cBa are larger and form with higher penetrance than in flies expressing eyScer\UAS.cHa alone, and eye formation occurs in the genitalia in flies expressing both eyScer\UAS.cHa and eyaScer\UAS.cBa.
|Complementation & Rescue Data|
|Stocks ( 2 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 4 )|
Saccharomyces cerevisiae UAS construct a of Bonini
|Secondary FlyBase IDs|
|References ( 16 )|