Allele Dmel\eya^Scer\UAS.cBa

General Information
Symbol	Dmel\eya^Scer\UAS.cBa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Bonini	FlyBase ID	FBal0083045
Feature type	allele	Associated gene	Dmel\eya

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Bonini et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: A full-length eya type I cDNA is expressed under the control of Scer\UAS regulatory sequences. (Bonini et al., 1997)
Carried in construct	P{UAS-eya.B.I} (Bonini et al., 1997, Zimmerman et al., 2000, Medioni and Noselli, 2005, Warrick et al., 1999, Pauli et al., 2005, Katsuyama et al., 2005, Anderson et al., 2006, Bai and Montell, 2002, Curtiss and Mlodzik, 2000, Bui et al., 2000, Bui et al., 2000, Seimiya and Gehring, 2000, Czerny et al., 1999, Clark et al., 2006, Blanco et al., 2010, Salzer et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| pupal stage, with Scer\GAL4^dpp.blk1 (Bonini et al., 1997) visible, with Scer\GAL4^dpp.blk1 (Seimiya and Gehring, 2000, Curtiss and Mlodzik, 2000, Bonini et al., 1997) visible, with Scer\GAL4^ey.PH (Curtiss and Mlodzik, 2000)
Phenotype Manifest In
	antennal segment, with Scer\GAL4^dpp.blk1 (Bonini et al., 1997) antennal segment & eye \| ectopic, with Scer\GAL4^dpp.blk1 (Bonini et al., 1997) egg chamber & basement membrane \| apical, with Scer\GAL4^upd (Medioni and Noselli, 2005) eye, with Scer\GAL4^ey.PH (Curtiss and Mlodzik, 2000) eye \| ectopic, with Scer\GAL4^dpp.blk1 (Bui et al., 2000, Seimiya and Gehring, 2000, Curtiss and Mlodzik, 2000, Bonini et al., 1997) interfollicle cell, with Scer\GAL4^hs.PB (Bai and Montell, 2002) leg, with Scer\GAL4^dpp.blk1 (Bonini et al., 1997) leg & ommatidium \| ectopic, with Scer\GAL4^dpp.blk1 (Bonini et al., 1997) ommatidium, with Scer\GAL4^ey.PH (Curtiss and Mlodzik, 2000) ommatidium \| ectopic, with Scer\GAL4^dpp.blk1 (Bonini et al., 1997) polar follicle cell, with Scer\GAL4^upd (Bai and Montell, 2002, Medioni and Noselli, 2005) wing, with Scer\GAL4^dpp.blk1 (Bonini et al., 1997) wing & ommatidium \| ectopic, with Scer\GAL4^dpp.blk1 (Bonini et al., 1997)
Detailed Description
	Statement Reference Overexpression of eya^Scer\UAS.cBa using Scer\GAL4^upd blocks anterior polar cell differentiation in egg chambers. As a consequence, the apical cap, which is formed from basement membrane components, that covers anterior polar cells in wild-type egg chambers is not present. (Medioni and Noselli, 2005) Expression of eya^Scer\UAS.cBa under the control of Scer\GAL4^hs.PB results in compound egg chambers which are not separated by interfollicular stalks and which are not always completely enveloped by the somatic epithelial layer. No germ cells are connected to each other by more than four ring canals indicating that there has not been an extra round of cell division. Expression of eya^Scer\UAS.cBa under the control of Scer\GAL4^upd results in one-third of egg chambers lacking polar cells at one end. (Bai and Montell, 2002) Expression of eya^Scer\UAS.cBa driven by Scer\GAL4^dpp.blk1 causes ectopic retinal development, leading to the eventual formation of ectopic compound eyes in the adult. (Bui et al., 2000) Expression of eya^Scer\UAS.cBa under the control of Scer\GAL4^ey.PH results in flies with smaller eyes than normal (which contain fewer facets than wild-type eyes). The regular arrangement of facets is lost in these eyes. Expression of eya^Scer\UAS.cBa under the control of Scer\GAL4^dpp.blk1 results in the induction of ectopic eyes. (Curtiss and Mlodzik, 2000) Ectopic eyes are produced at a frequency of 10% if eya^Scer\UAS.cBa is expressed under the control of Scer\GAL4^dpp.blk1. (Seimiya and Gehring, 2000) 10% of flies carrying a single copy of eya^Scer\UAS.cBa expressed under the control of a single copy of Scer\GAL4^dpp.blk1 in a wild-type background have ectopic eyes on the antennal segment which resemble normal eyes. These ectopic eyes contain photoreceptor cells in a pattern similar to that of the normal compound eye. 96% of flies carrying two copies of eya^Scer\UAS.cBa expressed under the control of two copies of Scer\GAL4^dpp.blk1 in a wild-type background have ectopic eyes on the antennae. 80% of these flies also show ommatidial formation on the legs, and occasionally on the wings. (Bonini et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa is an enhancer of visible phenotype of Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa (Bonini et al., 1997)
Suppressor of
Statement Reference Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa is a suppressor \| partially of visible \| recessive phenotype of dpp^d-blk (Curtiss and Mlodzik, 2000)
NOT Suppressor of
Statement Reference eya^Scer\UAS.cBa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Warrick et al., 1999)
Other
Statement Reference Scer\GAL4^dpp.blk1, eya^Scer\UAS.cBa, so^Scer\UAS.cPa has visible phenotype (Seimiya and Gehring, 2000, Bui et al., 2000, Bui et al., 2000, Seimiya and Gehring, 2000)
Phenotype Manifest In
Enhancer of
Statement Reference Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa is an enhancer of antenna phenotype of Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa (Bonini et al., 1997) Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa is an enhancer of haltere phenotype of Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa (Bonini et al., 1997) Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa is an enhancer of leg phenotype of Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa (Bonini et al., 1997) Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa is an enhancer of proboscis phenotype of Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa (Bonini et al., 1997) Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa is an enhancer of wing phenotype of Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa (Bonini et al., 1997)
Suppressor of
Statement Reference Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa is a suppressor \| partially of eye disc phenotype of dpp^d12 (Curtiss and Mlodzik, 2000) Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa is a suppressor \| partially of eye disc phenotype of dpp^d-blk (Curtiss and Mlodzik, 2000) Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa is a suppressor \| partially of eye phenotype of dpp^d-blk (Curtiss and Mlodzik, 2000) Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa is a suppressor \| partially of morphogenetic furrow phenotype of dpp^d-blk (Curtiss and Mlodzik, 2000) Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa is a suppressor \| partially of ommatidium phenotype of dpp^d12 (Curtiss and Mlodzik, 2000) Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa is a suppressor \| partially of ommatidium phenotype of dpp^d-blk (Curtiss and Mlodzik, 2000) Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa is a suppressor \| somatic clone of eye disc \| posterior \| somatic clone phenotype of Mad^1-2 (Curtiss and Mlodzik, 2000) Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa is a suppressor \| somatic clone of photoreceptor cell \| somatic clone phenotype of Mad^1-2 (Curtiss and Mlodzik, 2000)
NOT Suppressor of
Statement Reference eya^Scer\UAS.cBa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Warrick et al., 1999)
Other
Statement Reference Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa, eya^Scer\UAS.cBa has genitalia phenotype (Bonini et al., 1997, Bonini et al., 1997) Scer\GAL4^dpp.blk1, eya^Scer\UAS.cBa, so^Scer\UAS.cPa has antenna phenotype (Bui et al., 2000, Bui et al., 2000) Scer\GAL4^dpp.blk1, eya^Scer\UAS.cBa, so^Scer\UAS.cPa has eye \| ectopic phenotype (Seimiya and Gehring, 2000, Bui et al., 2000, Bui et al., 2000, Bui et al., 2000, Bui et al., 2000, Seimiya and Gehring, 2000) Scer\GAL4^dpp.blk1, eya^Scer\UAS.cBa, so^Scer\UAS.cPa has leg phenotype (Bui et al., 2000, Bui et al., 2000) Scer\GAL4^dpp.blk1, eya^Scer\UAS.cBa, so^Scer\UAS.cPa has wing phenotype (Bui et al., 2000, Bui et al., 2000) Scer\GAL4^twi.PG, Six4^Scer\UAS.cCa, eya^Scer\UAS.cBa has embryonic somatic muscle phenotype (Clark et al., 2006) Scer\GAL4^twi.PG, Six4^Scer\UAS.cCa, eya^Scer\UAS.cBa has fat body/gonad primordium phenotype (Clark et al., 2006) Scer\GAL4^twi.PG, Six4^Scer\UAS.cCa, eya^Scer\UAS.cBa has mesoderm phenotype (Clark et al., 2006) Scer\GAL4^twi.PG, Six4^Scer\UAS.cCa, eya^Scer\UAS.cBa has trunk mesoderm phenotype (Clark et al., 2006) Scer\GAL4^twi.PG, Six4^Scer\UAS.cCa, eya^Scer\UAS.cBa has ventral oblique muscle phenotype (Clark et al., 2006) Scer\GAL4^twi.PG, Six4^Scer\UAS.cCa, eya^Scer\UAS.cBa has visceral mesoderm phenotype (Clark et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of eya^Scer\UAS.cBa and Six4^Scer\UAS.cCa in the twi domain, under the control of Scer\GAL4^twi.PG strongly affects the mesoderm. Several cell types derived from the ventral and dorsolateral mesoderm expand. Greatly increased numbers of somatic gonadal precursors results in an enlarged gonad. There are also more fat body precursors, and these now extend into the dorsal region of the mesoderm, suggesting that dorsal cells have taken on more ventral fates. In contrast, derivatives of the dorsal mesoderm are disrupted. In stage 17 embryos no myosin-expressing cardioblasts are visible, while there are gaps in the pattern of dorsal muscles. Similarly, there are gaps in the visceral mesoderm at stage 12. In the ventral region of the somatic musculature, there are duplications of the ventral acute muscle (VA3), while the pattern of ventral oblique muscles is disrupted. The lateral somatic musculature also appears larger. (Clark et al., 2006) Expression of eya^Scer\UAS.cBa and so^Scer\UAS.cPa driven by Scer\GAL4^dpp.blk1 causes ectopic retinal development, leading to the eventual formation of ectopic compound eyes in the adult. (Bui et al., 2000) Co-expression of eya^Scer\UAS.cBa and so^Scer\UAS.cPa under the control of Scer\GAL4^dpp.blk1 results in ectopic eye tissue at many sites, including the antenna, legs and wings. (Bui et al., 2000) The dpp^d-blk phenotype is partially rescued by eya^Scer\UAS.cBa expressed under the control of Scer\GAL4^dpp.blk1. The small size of dpp^d-blk/dpp^d12 eye discs is partially rescued by eya^Scer\UAS.cBa expressed under the control of Scer\GAL4^dpp.blk1 and greater numbers of developing ommatidia are seen. Eye differentiation is rescued in Mad^1-2 clones that touch the posterior margin of the eye disc when eya^Scer\UAS.cBa is expressed under the control of Scer\GAL4^dpp.blk1. (Curtiss and Mlodzik, 2000) Ectopic eyes are produced at a frequency of 60% if eya^Scer\UAS.cBa and so^Scer\UAS.cPa are coexpressed under the control of Scer\GAL4^dpp.blk1. (Seimiya and Gehring, 2000) Ectopic eye formation in flies expressing ey^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 is greatly enhanced if the flies also carry eya^Scer\UAS.cBa; the ectopic eyes formed in flies expressing both ey^Scer\UAS.cHa and eya^Scer\UAS.cBa are larger and form with higher penetrance than in flies expressing ey^Scer\UAS.cHa alone, and eye formation occurs in the genitalia in flies expressing both ey^Scer\UAS.cHa and eya^Scer\UAS.cBa. (Bonini et al., 1997)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^ey.PB/eya^Scer\UAS.cBa rescues eya² (Bui et al., 2000) Scer\GAL4^unspecified/eya^Scer\UAS.cBa rescues eya¹ (Zimmerman et al., 2000) Scer\GAL4^unspecified/eya^Scer\UAS.cBa rescues eya² (Zimmerman et al., 2000) Scer\GAL4^unspecified/eya^Scer\UAS.cBa rescues eya⁴ (Zimmerman et al., 2000)
Partially rescues	Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa partially rescues eya² (Bonini et al., 1997) Scer\GAL4^ey.PB/eya^Scer\UAS.cBa partially rescues eya² (Bonini et al., 1997) Scer\GAL4^eya.PB/eya^Scer\UAS.cBa partially rescues eya² (Bui et al., 2000) Scer\GAL4^T59/eya^Scer\UAS.cBa partially rescues eya² (Bonini et al., 1997) Scer\GAL4^T155/eya^Scer\UAS.cBa partially rescues eya² (Bonini et al., 1997)
Comments	In eya², eya^Scer\UAS.cBa, Scer\GAL4^eya.PB flies the eye is restored. (Bui et al., 2000)
Stocks ( 2 )
Bloomington	5676 w¹¹¹⁸; P{UAS-eya.B.I}55.2
Kyoto	108356 w¹¹¹⁸; P{UAS-eya.B.I}55.2
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	cli^Scer\UAS.cBa cli^UAS.cBa eya^Scer\UAS.cBa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Bonini (Bonini et al., 1997)
Secondary FlyBase IDs

References ( 16 )
Research paper	Blanco et al., 2010, Dev. Biol. 344(2): 1088--1099 Genetic interactions of eyes absent, twin of eyeless and orthodenticle regulate sine oculis expression during ocellar development in Drosophila. [FBrf0211396] Salzer et al., 2010, Genetics 184(1): 185--197 The retinal determination gene eyes absent is regulated by the EGF receptor pathway throughout development in Drosophila. [FBrf0209690] Anderson et al., 2006, Dev. Biol. 297(2): 536--549 Regulation of the retinal determination gene dachshund in the embryonic head and developing eye of Drosophila. [FBrf0193889] Clark et al., 2006, Dev. Biol. 294(1): 220--231 D-six4 plays a key role in patterning cell identities deriving from the Drosophila mesoderm. [FBrf0190104] Katsuyama et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(44): 15918--15923 Involvement of winged eye encoding a chromatin-associated bromo-adjacent homology domain protein in disc specification. [FBrf0191445] Medioni and Noselli, 2005, Development 132(13): 3069--3077 Dynamics of the basement membrane in invasive epithelial clusters in Drosophila. [FBrf0187473] Pauli et al., 2005, Development 132(12): 2771--2782 Identification of functional sine oculis motifs in the autoregulatory element of its own gene, in the eyeless enhancer and in the signalling gene hedgehog. [FBrf0187477] Bai and Montell, 2002, Development 129(23): 5377--5388 Eyes Absent, a key repressor of polar cell fate during Drosophila oogenesis. [FBrf0151937] Bui et al., 2000, Dev. Biol. 221(2): 355--364 Functional analysis of an eye enhancer of the Drosophila eyes absent gene: differential regulation by eye specification genes. [FBrf0127029] Bui et al., 2000, Genetics 155(2): 709--720 Molecular analysis of Drosophila eyes absent mutants reveals features of the conserved eya domain. [FBrf0128419] Curtiss and Mlodzik, 2000, Development 127(6): 1325--1336 Morphogenetic furrow initiation and progression during eye development in Drosophila: the roles of decapentaplegic, hedgehog and eyes absent. [FBrf0125176] Seimiya and Gehring, 2000, Development 127(9): 1879--1886 The Drosophila homeobox gene optix is capable of inducing ectopic eyes by an eyeless-independent mechanism. [FBrf0127314] Zimmerman et al., 2000, Genetics 154(1): 237--246 Molecular genetic analysis of Drosophila eyes absent mutants reveals an eye enhancer element. [FBrf0123262] Czerny et al., 1999, Mol. Cell 3(3): 297--307 twin of eyeless, a second Pax-6 gene of Drosophila, acts upstream of eyeless in the control of eye development. [FBrf0107668] Warrick et al., 1999, Nat. Genet. 23(4): 425--428 Suppression of polyglutamine-mediated neurodegeneration in Drosophila by the molecular chaperone HSP70. [FBrf0123236] Bonini et al., 1997, Development 124(23): 4819--4826 The Drosophila eyes absent gene directs ectopic eye formation in a pathway conserved between flies and vertebrates. [FBrf0099887]

Allele Dmel\eyaScer\UAS.cBa

Allele Dmel\eya^Scer\UAS.cBa