FB2021_01, released Feb 18, 2021

Allele: Dmel\bonS024108

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General Information
Symbol
Dmel\bonS024108
Species
D. melanogaster
Name
FlyBase ID
FBal0083060
Feature type
allele
Associated gene
Dmel\bon
Associated Insertion(s)
P{lacW}bonS024108
Carried in Construct
Also Known As
bon241
Genomic Maps
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Delta2-3 transposase
(Deak et al., 1997)
P-element activity
(Szeged Stock Center, 1998-, Salzberg et al., 1997)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
transposable element insertion site
3R:20,593,166..20,593,166 [-]
(Beckstead et al., 2001, Prokopenko et al., 2000, Szeged Stock Center, 1998-, Deak et al., 1997, Salzberg et al., 1997)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
AF174658
AF174659
AF174660
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Cytology
      Nature of the lesion
      Statement
      Reference

      P{lacW} insertion in the 5' end of exon 1.

      (Beckstead et al., 2001)

      P{lacW} insertion in the 5' UTR, 686bp upstream of the bon AUG codon.

      (Prokopenko et al., 2000)
      Insertion components
      P{lacW}bonS024108
      Product class / Tool use(s)
      Encoded product / tool
      lacZ
      Tagged with
      Tag:NLS(P)
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous males die as first instar larvae, while homozygous females survive at least to the second larval instar stage. bonS024108/+ suppresses the variegation of the y gene in the P{SUPor-P}25-4-3 insertion.

      (Beckstead et al., 2005)

      34% of homozygotes survive to the pharate adult stage. bonS024108/bonS048706 pharate adults show defects in cuticle and bristle development; the abdominal cuticle is immature and the tergite and sternal bristles are severely reduced or absent. The anterior wing margin bristles are almost completely missing. There is a dramatic reduction in pigmentation of the wing cuticle.

      (Beckstead et al., 2001)

      Embryos exhibit two extra neurons located anteriorly to each dorsal cluster and one or two very large and round cells darkly stained with the MAb 22C10 are found posterior to the v' cluster.

      (Salzberg et al., 1997)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Statement
      Reference

      bonS024108 has lethal | recessive | male | first instar larval stage phenotype, non-suppressible by Sxlf1

      (Beckstead et al., 2005)
      Suppressor of
      Statement
      Reference

      bonS024108/bon[+] is a suppressor | partially of lethal phenotype of ftz-f1ex17/Df(3L)Cat

      (Beckstead et al., 2001)

      bonS024108/bon[+] is a suppressor | partially of partially lethal | recessive phenotype of ftz-f1ex17

      (Beckstead et al., 2001)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The lethality of bonS024108 homozygous males is not rescued if they are also carrying Sxlf1; no Sxlf1/Y ; bonS024108/bonS024108 male animals survive beyond the first larval instar.

      (Beckstead et al., 2005)

      70% of ftz-f1ex17/Df(3L)Cat animals are rescued to the pharate adult stage by one copy bonS024108. The number of homozygous ftz-f1ex17 adult escapers is increased by one copy of bonS024108 (60%).

      (Beckstead et al., 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      bon21B

      (Beckstead et al., 2001)
      Partially rescued by

      bonS024108/bonS048706 is partially rescued by bonHsp83.Tag:FLAG

      (Beckstead et al., 2001)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Allelic series: Df(3R)H-B79 > bon21B > bonS048706 > bonS024108 = bonS024912.

      (Beckstead et al., 2001)

      Δ2-3 induced reversion demonstrates the insertion is responsible for the lethal phenotype.

      (Salzberg et al., 1997)
      External Crossreferences and Linkouts ( 3 )
      Crossreferences
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      0241/08
      (Deak et al., 1997)
      241/8
      (Salzberg et al., 1997)
      bon241
      (Beckstead et al., 2005, Beckstead et al., 2001)
      bonS024108
      l(3)S024108
      (Prokopenko et al., 2000)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)