Allele Dmel\R^CD3

General Information
Symbol	Dmel\R^CD3	Species	D. melanogaster
Name		FlyBase ID	FBal0083270
Feature type	allele	Associated gene	Dmel\R
Also Known As	Rap1^CD3

Allele class
Mutagen
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference 4.5kb deletion that removes R coding region. (Asha et al., 1999)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| germline clone \| non-rescuable maternal effect (Asha et al., 1999)
Phenotype Manifest In
	dorsal closure embryo \| germline clone \| maternal effect (Asha et al., 1999) embryonic/first instar larval cuticle (Sawyer et al., 2009) embryonic/larval dorsal vessel \| germline clone \| maternal effect (Asha et al., 1999) embryonic head \| germline clone \| maternal effect (Asha et al., 1999) eye (Asha et al., 1999) gastrula embryo \| germline clone \| maternal effect (Asha et al., 1999) mesoderm \| germline clone \| maternal effect (Asha et al., 1999) pole cell \| germline clone \| maternal effect (Asha et al., 1999) ventral furrow \| germline clone \| maternal effect (Asha et al., 1999) ventral nerve cord \| germline clone \| maternal effect (Asha et al., 1999)
Detailed Description
	Statement Reference Loss of maternal and zygotic R in R[CD3] germ-line clones disrupts ventral cuticle resulting in twi-positive mesoderm remaining on the surface after gastrulation. In some R[CD3] mutants the germband becomes twisted during gastrulation. Coupling between actomyosin constriction and cell shape change is disrupted in R[CD3] mutants. Balls of actin and myosin appear at the apical surface of mesodermal cells, and cell constriction halts prematurely, with myosin balls not contiguous with adherens junctions. (Sawyer et al., 2009) Mutant clones in the eye show no defect at early pupal stages. But in the resulting adult eyes scars indicate that mutant tissue does not survive to the adult stage. Remnants of rhabdomere are present in the degenerated tissue. Mutants derived from germline clones show (non-paternally-rescuable) embryonic lethality. Affected embryos show normal axis specification, including for the terminal system. Gastrulation is affected, for 50% of embryos the ventral furrow never closes, although the mesodermal precursors display the usual apical construction and membrane blebbing. At the time of ventral furrow formation nuclei of invaginating cells are found at a variety of levels, instead of in the more usual basal location. Dorsal migration of internalised mesoderm is less extensive than in wild type. Dorsal structures such as the dorsal vessel do not form. Tracheal development seems relatively unaffected. Pole cell migration is severely impaired. Only a small number of cells reach the gonad. Head involution frequently fails and dorsal closure fails 5-10% of the time. These defects are decreased by paternally rescuing R⁺. The cytoskeleton in gastrulating embryos seems relatively normal. (Asha et al., 1999)
External Data
Linkouts
Interactions
Phenotypic Class
NOT Enhancer of
Statement Reference R[+]/R^CD3 is a non-enhancer of visible phenotype of CycE^JP (Brumby et al., 2004)
NOT Suppressor of
Statement Reference R[+]/R^CD3 is a non-suppressor of visible phenotype of CycE^JP (Brumby et al., 2004)
Phenotype Manifest In
NOT Enhancer of
Statement Reference R[+]/R^CD3 is a non-enhancer of eye phenotype of CycE^JP (Brumby et al., 2004)
NOT Suppressor of
Statement Reference R[+]/R^CD3 is a non-suppressor of eye phenotype of CycE^JP (Brumby et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	R^CD3 Rap1^1CD3 (Mishra et al., 2005) Rap1^CD3 (Brumby et al., 2004, Asha et al., 1999, Li et al., 1997, Sawyer et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Sawyer et al., 2009, J. Cell Biol. 186(1): 57--73 The Drosophila afadin homologue Canoe regulates linkage of the actin cytoskeleton to adherens junctions during apical constriction. [FBrf0208505] Mishra et al., 2005, Curr. Biol. 15(4): 366--370 Ras-independent activation of ERK signaling via the torso receptor tyrosine kinase is mediated by Rap1. [FBrf0183834] Brumby et al., 2004, Genetics 168(1): 227--251 A genetic screen for dominant modifiers of a cyclin E hypomorphic mutation identifies novel regulators of S-phase entry in Drosophila. [FBrf0180291] Asha et al., 1999, EMBO J. 18(3): 605--615 The Rap1 GTPase functions as a regulator of morphogenesis in vivo. [FBrf0106166] Li et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94(23): 12515--12520 Genetic interactions with rap1 and ras1 reveal a second function for the fat facets deubiquitinating enzyme in Drosophila eye development. [FBrf0099363]

Allele Dmel\RCD3

Allele Dmel\R^CD3